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Serum apolipoprotein b-48 concentration are correlated with plaque score of carotid arteries
Abstracts / Atherosclerosis 235 (2014) e84–e191
Methods: Two examinations were carried out in six healthy male volunteers – they received an experimental breakfast containing 75 g of fat (cream) or 75 g of fat + 25 g of carbohydrate. The concentrations of insulin, glucose, triglyceride and nonesterified fatty acids (NEFA) in plasma were measured within 2 hours after breakfast. The order of both examinations was randomized. Results: The addition of 25 g of carbohydrate to 75 g of fat induced a pronounced insulin response after 30 minutes (increase from 7 to 39 mIU/ l). The fat load alone affected neither insulinemia nor glycemia. There were no significant differences in the changes of concentration of triglyceride and NEFA in early postprandial phase. Conclusion: The addition of small amount of carbohydrate (25 g) to fat load as recommended recently (Kolovou et al, Curr Vasc Pharmacol 2011; 9: 258-70) induces physiological response of insulin and should be therefore preferred during postprandial lipemia testing. Supported by grant No. NT 14027-3/2013 from IGA MH CR. 28 - TG rich and post prandial lipoproteins EAS-0588. OXIDATIVE STRESS AND MRNA EXPRESSION AFTER AN ORAL UNSATURATED FAT LOAD TEST IN SUBJECTS WITH ABDOMINAL OBESITY S. Martinez-Hervasa, I. Navarroa, A.B. Garcia-Garciab, E. Nuñez Savalla, E. Benitoa, F.J. Chavesb, J.T. Reala, J.F. Ascasoa a Service of Endocrinology and Nutrition, Hospital Clinico Universitario de Valencia. University of Valencia. INCLIVA, Valencia, Spain; b Unidad de Genotipado y Diagnóstico Genétcio, INCLIVA, Valencia, Spain
Objectives: Insulin resistance and oxidative stress are related to increased atherosclerosis. Our aim was to analyze levels of oxidative stress and lympho-monocytes mRNA expression profile in subjects with abdominal obesity and healthy controls as well as the influence of an oral unsaturated fat load test in both, oxidative stress status and mRNA profile. Methods: We included 20 subjects with abdominal obesity (waist circumference > 102 cm for men and > 88 cm for women; fasting plasma glucose < 126 mg/dl) and 20 healthy controls (waist circumference < 102 cm for men and < 88 cm for women; fasting plasma glucose < 100 mg/dl: total cholesterol < 200 mg/dl, and triglycerides < 150 mg/dl). After 12 hours of fasting we performed a standardized fat load test (0-8 hours) with supracalÒ (50 g/m2). mRNA was analyzed by Illumina system iScan. Results: the subjects with obesity showed significant higher levels of basal HOMA (1.10.6 vs 4.63.9, respectively for controls and obese subjects; p<0.01) and oxidative stress levels (GSSG/GSH ratio 0.0250.005 vs 0.0680.019, respectively for controls and obese subjects; p<0.0001). Triglycerides rose after the administration of the unsaturated fat, achieving the maximum at 4 hours. However, HOMA and GSSG/GSH ratio showed significant decrease.
Ratio GSSG/GSH
Control group
Abdominal obesity group
p
Basal
0,0250,005
0,0680,019
0,0001
SOGr 4h
0,0130,003
0,0270,006
0,0001
SOGr 8h
0,0090,002
0,0240,041
0.1257
Subjects with abdominal obesity showed a significant decrease in the mRNA expression of FKBP5 (p¼0.0027) and DDIT4 (p¼0.0038) genes.
Conclusion: we have found that unsaturated fat improves insulin resistance and oxidative stress status together with modification of the gene expression of systems related to oxidative stress and immunomodulation. Thus, the consumption of unsaturated fat could be beneficial even in subjects with obesity. Funding: Instituto de Salud Carlos III (ISCIII) FIS PI10/00511
e169
28 - TG rich and post prandial lipoproteins EAS-0468. SERUM APOLIPOPROTEIN B-48 CONCENTRATION ARE CORRELATED WITH PLAQUE SCORE OF CAROTID ARTERIES M. Okuboa, H. Hanadaa, M. Matsuia, Y. Hidakab, D. Masudac, Y. Sakatac, S. Yamashitad a Department of Medical Technology, Osaka University Hospital, Suita Osaka, Japan; b Department of Laboratory Medicine, Osaka University Hospital, Suita Osaka, Japan; c Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine, Suita Osaka, Japan; d Department of Community Medicine, Osaka University Graduate School of Medicine, Suita Osaka, Japan
Objectives: Postprandial hypertriglyceridemia (PHTG) refers to a state of postprandial accumulation of intestine-derived lipoproteins, such as chylomicrons (CM) and chylomicron remnants (CM-R). PHTG is one of the independent risk factors for coronary heart disease (CHD) since CM-R have an atherogenic nature. We established a chemiluminescence enzyme immunoassay for measuring serum concentration of apolipoprotein B-48 (apoB-48) which is a constituent of CM and CM-R, and reported that high apoB-48 concentration correlated with CHD prevalence. In the present study, whether serum apoB-48 concentration correlated with subclinical atherosclerosis has not been examined. Methods: A total of 163 subjects were enrolled. Blood pressure (BP) was measured and serum concentrations of apoB-48, plasma glucose, HbA1c, TC, TG HDL-C and LDL-C were measured. Intima-media thickness (IMT) of carotid arteries was measured and plaque score (PS) of carotid arteries were calculated in all subjects. Results: All subjects were classified into 4 groups by apoB-48 concentration quartiles: 1st quartile (0-2.2 mg/mL, n¼41), 2nd quartile (2.3-3.5 mg/mL, n¼39), 3rd quartile (3.6-6.7 mg/mL, n¼42) and 4th quartile (?6.8 mg/mL, n¼41), respectively. TC, TG, LDL-C concentrations were increased and HDL-C was decreased as the increase of apoB-48 concentrations. Max-IMT and PS were significantly higher in subjects of 4th quartile than those of 1st, 2nd and 3rd quartile and had a significant correlation with age, eGFR, systolic BP, logTG and log-apoB-48 levels. A multiple logistic regression analysis revealed that Max-IMT and PS significantly correlated with age, eGFR, and log-apoB48 concentrations (p<0.05). In subjects whose TG concentrations were within normal range (<150 mg/dL), max-IMT and PS were significantly higher in subjects of 4th quartile than those of 1st, 2nd and 3rd quartile. Conclusion: Serum apoB-48 concentrations strongly correlated with maxIMT and PS, indicating that increased CM-R might contribute to an increased risk of subclinical carotid atherosclerosis. 28 - TG rich and post prandial lipoproteins EAS-0269. A NOVEL POTENT AND SELECTIVE PPARALPHA AGONIST, K-877, AMERIOLATES BOTH FASTING AND POSTPRANDIAL HYPERTRIGLYCERIDEMIA D. Masudaa, T. Kobayashia, T. Okadaa, H. Nakaokaa, R. Kawasea, K. Nakatania, M. Kosekia, T. Ohamab, M. Nishidab, Y. Sakataa, S. Yamashitac a Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine, Suita Osaka, Japan; b Health Care Center, Osaka University, Toyonaka Osaka, Japan; c Department of Community Medicine, Osaka University Graduate School of Medicine, Suita Osaka, Japan
Objectives: Fasting and postprandial hypertriglyceridemia (PHTG) is caused by the impaired metabolism of TG-rich lipoproteins and their remnant lipoproteins. Since remnant lipoprotein is highly atherogenic, the improvement of impaired metabolism of remnant lipoproteins is important for preventing atherosclerotic cardiovascular events. In the current study, we investigated the effect of a potent and selective peroxisome proliferator-activated receptor alpha (PPARa) agonist, K-877, on TG-rich lipoprotein metabolism.
Methods: Two examinations were carried out in six healthy male volunteers – they received an experimental breakfast containing 75 g of fat (cream) or 75 g of fat + 25 g of carbohydrate. The concentrations of insulin, glucose, triglyceride and nonesterified fatty acids (NEFA) in plasma were measured within 2 hours after breakfast. The order of both examinations was randomized. Results: The addition of 25 g of carbohydrate to 75 g of fat induced a pronounced insulin response after 30 minutes (increase from 7 to 39 mIU/ l). The fat load alone affected neither insulinemia nor glycemia. There were no significant differences in the changes of concentration of triglyceride and NEFA in early postprandial phase. Conclusion: The addition of small amount of carbohydrate (25 g) to fat load as recommended recently (Kolovou et al, Curr Vasc Pharmacol 2011; 9: 258-70) induces physiological response of insulin and should be therefore preferred during postprandial lipemia testing. Supported by grant No. NT 14027-3/2013 from IGA MH CR. 28 - TG rich and post prandial lipoproteins EAS-0588. OXIDATIVE STRESS AND MRNA EXPRESSION AFTER AN ORAL UNSATURATED FAT LOAD TEST IN SUBJECTS WITH ABDOMINAL OBESITY S. Martinez-Hervasa, I. Navarroa, A.B. Garcia-Garciab, E. Nuñez Savalla, E. Benitoa, F.J. Chavesb, J.T. Reala, J.F. Ascasoa a Service of Endocrinology and Nutrition, Hospital Clinico Universitario de Valencia. University of Valencia. INCLIVA, Valencia, Spain; b Unidad de Genotipado y Diagnóstico Genétcio, INCLIVA, Valencia, Spain
Objectives: Insulin resistance and oxidative stress are related to increased atherosclerosis. Our aim was to analyze levels of oxidative stress and lympho-monocytes mRNA expression profile in subjects with abdominal obesity and healthy controls as well as the influence of an oral unsaturated fat load test in both, oxidative stress status and mRNA profile. Methods: We included 20 subjects with abdominal obesity (waist circumference > 102 cm for men and > 88 cm for women; fasting plasma glucose < 126 mg/dl) and 20 healthy controls (waist circumference < 102 cm for men and < 88 cm for women; fasting plasma glucose < 100 mg/dl: total cholesterol < 200 mg/dl, and triglycerides < 150 mg/dl). After 12 hours of fasting we performed a standardized fat load test (0-8 hours) with supracalÒ (50 g/m2). mRNA was analyzed by Illumina system iScan. Results: the subjects with obesity showed significant higher levels of basal HOMA (1.10.6 vs 4.63.9, respectively for controls and obese subjects; p<0.01) and oxidative stress levels (GSSG/GSH ratio 0.0250.005 vs 0.0680.019, respectively for controls and obese subjects; p<0.0001). Triglycerides rose after the administration of the unsaturated fat, achieving the maximum at 4 hours. However, HOMA and GSSG/GSH ratio showed significant decrease.
Ratio GSSG/GSH
Control group
Abdominal obesity group
p
Basal
0,0250,005
0,0680,019
0,0001
SOGr 4h
0,0130,003
0,0270,006
0,0001
SOGr 8h
0,0090,002
0,0240,041
0.1257
Subjects with abdominal obesity showed a significant decrease in the mRNA expression of FKBP5 (p¼0.0027) and DDIT4 (p¼0.0038) genes.
Conclusion: we have found that unsaturated fat improves insulin resistance and oxidative stress status together with modification of the gene expression of systems related to oxidative stress and immunomodulation. Thus, the consumption of unsaturated fat could be beneficial even in subjects with obesity. Funding: Instituto de Salud Carlos III (ISCIII) FIS PI10/00511
e169
28 - TG rich and post prandial lipoproteins EAS-0468. SERUM APOLIPOPROTEIN B-48 CONCENTRATION ARE CORRELATED WITH PLAQUE SCORE OF CAROTID ARTERIES M. Okuboa, H. Hanadaa, M. Matsuia, Y. Hidakab, D. Masudac, Y. Sakatac, S. Yamashitad a Department of Medical Technology, Osaka University Hospital, Suita Osaka, Japan; b Department of Laboratory Medicine, Osaka University Hospital, Suita Osaka, Japan; c Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine, Suita Osaka, Japan; d Department of Community Medicine, Osaka University Graduate School of Medicine, Suita Osaka, Japan
Objectives: Postprandial hypertriglyceridemia (PHTG) refers to a state of postprandial accumulation of intestine-derived lipoproteins, such as chylomicrons (CM) and chylomicron remnants (CM-R). PHTG is one of the independent risk factors for coronary heart disease (CHD) since CM-R have an atherogenic nature. We established a chemiluminescence enzyme immunoassay for measuring serum concentration of apolipoprotein B-48 (apoB-48) which is a constituent of CM and CM-R, and reported that high apoB-48 concentration correlated with CHD prevalence. In the present study, whether serum apoB-48 concentration correlated with subclinical atherosclerosis has not been examined. Methods: A total of 163 subjects were enrolled. Blood pressure (BP) was measured and serum concentrations of apoB-48, plasma glucose, HbA1c, TC, TG HDL-C and LDL-C were measured. Intima-media thickness (IMT) of carotid arteries was measured and plaque score (PS) of carotid arteries were calculated in all subjects. Results: All subjects were classified into 4 groups by apoB-48 concentration quartiles: 1st quartile (0-2.2 mg/mL, n¼41), 2nd quartile (2.3-3.5 mg/mL, n¼39), 3rd quartile (3.6-6.7 mg/mL, n¼42) and 4th quartile (?6.8 mg/mL, n¼41), respectively. TC, TG, LDL-C concentrations were increased and HDL-C was decreased as the increase of apoB-48 concentrations. Max-IMT and PS were significantly higher in subjects of 4th quartile than those of 1st, 2nd and 3rd quartile and had a significant correlation with age, eGFR, systolic BP, logTG and log-apoB-48 levels. A multiple logistic regression analysis revealed that Max-IMT and PS significantly correlated with age, eGFR, and log-apoB48 concentrations (p<0.05). In subjects whose TG concentrations were within normal range (<150 mg/dL), max-IMT and PS were significantly higher in subjects of 4th quartile than those of 1st, 2nd and 3rd quartile. Conclusion: Serum apoB-48 concentrations strongly correlated with maxIMT and PS, indicating that increased CM-R might contribute to an increased risk of subclinical carotid atherosclerosis. 28 - TG rich and post prandial lipoproteins EAS-0269. A NOVEL POTENT AND SELECTIVE PPARALPHA AGONIST, K-877, AMERIOLATES BOTH FASTING AND POSTPRANDIAL HYPERTRIGLYCERIDEMIA D. Masudaa, T. Kobayashia, T. Okadaa, H. Nakaokaa, R. Kawasea, K. Nakatania, M. Kosekia, T. Ohamab, M. Nishidab, Y. Sakataa, S. Yamashitac a Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine, Suita Osaka, Japan; b Health Care Center, Osaka University, Toyonaka Osaka, Japan; c Department of Community Medicine, Osaka University Graduate School of Medicine, Suita Osaka, Japan
Objectives: Fasting and postprandial hypertriglyceridemia (PHTG) is caused by the impaired metabolism of TG-rich lipoproteins and their remnant lipoproteins. Since remnant lipoprotein is highly atherogenic, the improvement of impaired metabolism of remnant lipoproteins is important for preventing atherosclerotic cardiovascular events. In the current study, we investigated the effect of a potent and selective peroxisome proliferator-activated receptor alpha (PPARa) agonist, K-877, on TG-rich lipoprotein metabolism.