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Study on the distribution of placental protein 14 in human body
Abstracts:
JPA 97, Free
Communications
A.13
STUDY ON THE DISTRIBUTION OF PLACENTAL PROTEIN 14 IN HUMAN BODY. Y.Suzuki. R.Sugiyama, H.Ito, T.Iwaki, T.Yudate, KIsakaand M.Takayama, Department of Obstetrics and Gynecology, Tokyo Medical College Hospital, Tokyo, Japan Objective : Placental Protein 14 (PP14) was originally isolated from human term placenta by Bohn et al. in 1982. PPl4 has been suggested to have an important immunosuppressive activity during reproductive life. In this studv we observed expression of PP14 mRNA and PP14 localization in human endometrium. Method : Exoression of PP14 mRNA in eoithehal and stromal cells wh&h were extracted separately from endometrium was observed by the method of RT-PCR. PP14 localization in endometrium of proliferative phase and secretory phase, post menopausal endometrium and decidua was observed immunohistochemicalty. In this immunohistochemical studv we used anti PP14 oolvclonal antibodv which was kindly donated by B. Teis’ne; Results : PP14 mRNA was expressed in epithelial cells’ofmetrium but not in stromal cells: PP14 staining was found in 40% of grandular epithelium in the proliferative phase, and the staining was more prominent(90%) in the secretory phase. However PP14 stainimg was not seen in stroma throughout the menstrual cycle. In decidualized endometrium PP14 stained strongly in both epithelium and stroma. However in the endometrium of post menopausal women, PP14 staining was negative. Conclusion : We determined that PP14 mRNA was expressed in epithetial cells of endometrium, and PP14 production and secretion from endometrium is related to the menstrual cycle. It is suggested that PP14 is an important factor associated with the early stage of human reproduction. I
EXPRESSION OF POLO LIKE KINASE (PLK) IN THE MURINE PLACENTA, ENDOMETRIUM AND OVARY. N. Takai, J. Yoshimatsu, Y. Nishida, R. Hamanaka* and I. Miyakawa, Department of Obstetrics and Gynecology, 1st department of Biochemistry*, Oita medical university, Hasama-machi, Oita, Japan. [Objective] Human Polo like kinase(PLK) gene was reported by us as a new placenta-specific kinase gene ( Hamanaka et al. Cell Growth & Differ. 5:249-257, 1994. ). To further analyze in vivo expression, we have cloned a murine PLK gene, and examined the features of murine PLK expression in placenta, endometrium and ovary. [Methods and results] A new kinase gene with 2.1 kb nucleotide sequences was cloned from a murine thymus cDNA library. SDS-PAGE analysis detected the 65kDa PLK protein. Immunohistochemical study of the placenta, endometrium and ovary showed that PLK was strongly expressed in the trophobtast invading the decidua, the endometrium with implantation and the stroma of the ovary. [Conclusion] It is suggested that PLK, a newly cloned placenta-specific kinase, may play an important role in the active cytokinesis of the placenta, endometrium and ovary.
THE CORRELATION BETWEEN EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR AND ENDOTHELIAL CELL PROLIFERATION IN THE UTERUS OF EARLY GESTATION RATS. N. Tadokoro”‘, P. Rogers, D. Healy, N. Inaba”‘, Dokkyo University School of Medicine”‘, Tochigi, Japan, and Monash University School of Medicine, Sidney, Australia Endometrium in the early stage of pregnancy requires angiogenesis for the process of implantation and placental development, and vascular endothetial growth factor (VEGF) has been reported as one of the important angiogenesis factors. In the present report, the correlation between expression of VEGF and endothelial cell proliferation in the uterus of early gestation rats was studied. Rat uteri at day 1, 3, 5 and 7 of pregnacy were sampled (n=4), for immunohistochemicat study using anti-VEGF antibody, and northern analysis (NA) and RNase protection assay (RPA) using mouse VEGF cRNA probe. Positive staining of VEGF was observed in uterine cavity epithelium, gland epithelium, and myometrium, with no significant difference in the staining index, while in endometrial stroma, the index increased as the pregnancy proceeded, and reached a maximum in the decidual cells at day 7 of pregnancy. In NA study, an endometrial VEGF mRNA significant difference in tissue mRNA content in relation to the day of pregnancy. From the results obtained, we determine that VEGF, especially that produced in endometrial stroma, might be important among angiogenesis factors.
IMMUNORADIOMETRICAL MEASUREMENT OF CYTOKERATIN 19 FRAGMENT IN NORMAL AND COMPLICATED PREGNANT WOMEN. K. Yamanaka, T. Kihana, H. Ochi, K. Matsubara, H. Kitagawa, M. Ito, *Department of Obstetrics and Gynecology, Ehime University school of Medicine, Shigenobu, Japan. Ojective: The present study was conducted to investigate cytokeratin 19 fragment in normal and complicated pregnant women. Methods: We measured serum cytokeratin 19 fragment (CYFRA) values in serum samples obtained from the peripheral vein (n=202), uterine vein (n=5), and umbilical vein (n=15) in normal and complicated pregnant women (pregnancy induced hypertension and intrauterine growth retardation). The measurement was performed by radioimmunoassay using murine monoctonal antibodies, KS 19.1 and BM 19.21. Subsequently, the placentas were fixed with formaldehyde and immunostained with KS 19.1. Rusutts: The mean peripheral serum value of CYFRA in normal pregnant women increased significantly during pregnancy and decreased rapidly after delivery. The mean value of CYFRA in uterine vein was extremely higher than the peripheral vein. On the other hand the mean value in umbilical vein was lower than the oerioheral vein. The peripheral serum levels in complica’ted’pregnant women were higher than normal pregnant women. The immunocytochemical studies showed the trophoblast had CKI 9 staining and that infarct lesions had extremely dense CK19 staining. Conclusions: The origin of serum CYFRA during pregnancy could be the trophoblast of placenta and the placental infarction in complicated pregnancy might cause the elevation in serum CYFRA level.
JPA 97, Free
Communications
A.13
STUDY ON THE DISTRIBUTION OF PLACENTAL PROTEIN 14 IN HUMAN BODY. Y.Suzuki. R.Sugiyama, H.Ito, T.Iwaki, T.Yudate, KIsakaand M.Takayama, Department of Obstetrics and Gynecology, Tokyo Medical College Hospital, Tokyo, Japan Objective : Placental Protein 14 (PP14) was originally isolated from human term placenta by Bohn et al. in 1982. PPl4 has been suggested to have an important immunosuppressive activity during reproductive life. In this studv we observed expression of PP14 mRNA and PP14 localization in human endometrium. Method : Exoression of PP14 mRNA in eoithehal and stromal cells wh&h were extracted separately from endometrium was observed by the method of RT-PCR. PP14 localization in endometrium of proliferative phase and secretory phase, post menopausal endometrium and decidua was observed immunohistochemicalty. In this immunohistochemical studv we used anti PP14 oolvclonal antibodv which was kindly donated by B. Teis’ne; Results : PP14 mRNA was expressed in epithelial cells’ofmetrium but not in stromal cells: PP14 staining was found in 40% of grandular epithelium in the proliferative phase, and the staining was more prominent(90%) in the secretory phase. However PP14 stainimg was not seen in stroma throughout the menstrual cycle. In decidualized endometrium PP14 stained strongly in both epithelium and stroma. However in the endometrium of post menopausal women, PP14 staining was negative. Conclusion : We determined that PP14 mRNA was expressed in epithetial cells of endometrium, and PP14 production and secretion from endometrium is related to the menstrual cycle. It is suggested that PP14 is an important factor associated with the early stage of human reproduction. I
EXPRESSION OF POLO LIKE KINASE (PLK) IN THE MURINE PLACENTA, ENDOMETRIUM AND OVARY. N. Takai, J. Yoshimatsu, Y. Nishida, R. Hamanaka* and I. Miyakawa, Department of Obstetrics and Gynecology, 1st department of Biochemistry*, Oita medical university, Hasama-machi, Oita, Japan. [Objective] Human Polo like kinase(PLK) gene was reported by us as a new placenta-specific kinase gene ( Hamanaka et al. Cell Growth & Differ. 5:249-257, 1994. ). To further analyze in vivo expression, we have cloned a murine PLK gene, and examined the features of murine PLK expression in placenta, endometrium and ovary. [Methods and results] A new kinase gene with 2.1 kb nucleotide sequences was cloned from a murine thymus cDNA library. SDS-PAGE analysis detected the 65kDa PLK protein. Immunohistochemical study of the placenta, endometrium and ovary showed that PLK was strongly expressed in the trophobtast invading the decidua, the endometrium with implantation and the stroma of the ovary. [Conclusion] It is suggested that PLK, a newly cloned placenta-specific kinase, may play an important role in the active cytokinesis of the placenta, endometrium and ovary.
THE CORRELATION BETWEEN EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR AND ENDOTHELIAL CELL PROLIFERATION IN THE UTERUS OF EARLY GESTATION RATS. N. Tadokoro”‘, P. Rogers, D. Healy, N. Inaba”‘, Dokkyo University School of Medicine”‘, Tochigi, Japan, and Monash University School of Medicine, Sidney, Australia Endometrium in the early stage of pregnancy requires angiogenesis for the process of implantation and placental development, and vascular endothetial growth factor (VEGF) has been reported as one of the important angiogenesis factors. In the present report, the correlation between expression of VEGF and endothelial cell proliferation in the uterus of early gestation rats was studied. Rat uteri at day 1, 3, 5 and 7 of pregnacy were sampled (n=4), for immunohistochemicat study using anti-VEGF antibody, and northern analysis (NA) and RNase protection assay (RPA) using mouse VEGF cRNA probe. Positive staining of VEGF was observed in uterine cavity epithelium, gland epithelium, and myometrium, with no significant difference in the staining index, while in endometrial stroma, the index increased as the pregnancy proceeded, and reached a maximum in the decidual cells at day 7 of pregnancy. In NA study, an endometrial VEGF mRNA significant difference in tissue mRNA content in relation to the day of pregnancy. From the results obtained, we determine that VEGF, especially that produced in endometrial stroma, might be important among angiogenesis factors.
IMMUNORADIOMETRICAL MEASUREMENT OF CYTOKERATIN 19 FRAGMENT IN NORMAL AND COMPLICATED PREGNANT WOMEN. K. Yamanaka, T. Kihana, H. Ochi, K. Matsubara, H. Kitagawa, M. Ito, *Department of Obstetrics and Gynecology, Ehime University school of Medicine, Shigenobu, Japan. Ojective: The present study was conducted to investigate cytokeratin 19 fragment in normal and complicated pregnant women. Methods: We measured serum cytokeratin 19 fragment (CYFRA) values in serum samples obtained from the peripheral vein (n=202), uterine vein (n=5), and umbilical vein (n=15) in normal and complicated pregnant women (pregnancy induced hypertension and intrauterine growth retardation). The measurement was performed by radioimmunoassay using murine monoctonal antibodies, KS 19.1 and BM 19.21. Subsequently, the placentas were fixed with formaldehyde and immunostained with KS 19.1. Rusutts: The mean peripheral serum value of CYFRA in normal pregnant women increased significantly during pregnancy and decreased rapidly after delivery. The mean value of CYFRA in uterine vein was extremely higher than the peripheral vein. On the other hand the mean value in umbilical vein was lower than the oerioheral vein. The peripheral serum levels in complica’ted’pregnant women were higher than normal pregnant women. The immunocytochemical studies showed the trophoblast had CKI 9 staining and that infarct lesions had extremely dense CK19 staining. Conclusions: The origin of serum CYFRA during pregnancy could be the trophoblast of placenta and the placental infarction in complicated pregnancy might cause the elevation in serum CYFRA level.