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The evaluation of hormone in patient with atopic dermatitis
VI. Japanese Speaking Session Abstract
Symposium
(51-512)
Jl THE EVALUATION
OF
HORMONE
IN PATIENT
NITH ATOPIC
DERJ1ATITJ.S KIYOSHI Department
KAGAMI of Dermatolosy,
Kyoto First Red Cross
Hospital At birth, sebaceous deveroped as a result ulation
and
glands of
are moderately
maternal
hormonal
well stim-
much reduced in childin general, the sebaceous aland
sequentially
hood. I" puberty, deverop strlkinqly and 1s activated by the hormonal stimulation. In patient with atopic dermatitis the deficiency-of skin surface-lipids produced by sebaceous glands are noticed. The lower activity of sebaceous glands are presumably caused by the deficiency of hormones,such as androqe", estrogen or proqesterone. Jn this paper the value of hormone was estimated in patlent with atopic dermatitis compared with healthy controls.
54
5.3 RELEASE OF MACROPHAGE ANAPHYLACTIC REACTIONS
CHEMOTACTIC ACTIVITY IN HUMAN SKIN.
LA'TE PHASE REACTION
IN
OF THE SKIN
TAMOTSU KANZAKI AND HARUMI HORI DPpartment of Oermatoloqy, Nagoya Medlcal School, Nagoya
OSAMU KORO, SATORU YAMADA, ISAMU KIMURA, YASUSHI SUGITA AND SHOSO YAMAMOTO Dept. of Dermatology, Hiroshima Univ. School of Med. Hiroshima
City
un>.vers1ty
(LPR) IS an lmmedlate type Late phase reaction reactlo" but apprup of
It is known that mononuclear leukocytes accumulate in inflammatory lesion of atopic dermatitis. Tn the present study to investigate the mechanism of the accumulation, macrophage chemotactic activity (MCA) was determined in cutaneous anaphylactic reaction in patients with atopic MCA was dermatitis using skin chamber technique. remarkably increased in the chamber fluid obtained from the site with antigen at the interval of 1 hr up to 4 hr while at the control site there was no remarkable increase of MCA. The result shows that cutaneous anaphylactic reaction may evoke macrophage accumulation at the inflammatory lesion in the patients with atopic dermatitis.
dtoplc
dermatltls.
56
J5 RN IMMUNOHISTOLOGICAL
ANALYSLS OF WHEAT ALLERGEN IN ATOPLC UEKUTITIS ANU PKEPARATION OF HYPOALLEKGENICWHEAT
STUDY OF PATCH TEST LESIONS PROVOKED BY ACETONE. SOLUBLE HUMAN DANDER IN PA'PlENTS WITH ATOPIC DERMATITIS
Z. IKEZAWA, M. AHANO, M. KAGEYAMA,M. OKATU, K. TAKAHXHI, C. SUGA, A. SUGIYAMA, H. KOHATU, H. N&&JIMA AND K. TUBAKI* Departmentof Dermatology,Yokohama City Uni.Schoolof Medicine, Y,lkohama.*AsahidenkaInstitute.Tokyo We have already succeeded in preparationof hypaallrrgenit rice for elimination diet of rice in the patirncs of severe atopic dermatitis(AD)with rice allergy. We also revealedthat there are partial cross-reactionsbetween rice and wheat .antigens,and that wheae allergy may play an impurtant role in developemeneof severe AD in some cases. In this paper. we fractionatedthe wheat antigen proteins to water-, 1M N&l-, 70% ethanol-, and 4M urea-solublefracrions, and assayed IgE antibody tifer against rnch fraction. i-heresult:;showed thae 1M N&l-suluble fractionmay be major allergenic component of wheat. Then ,.,e prepared hypoallrrgenic wheat by decompositionof the fraction,and examined its clinical effect.
HISASHI SUGIL,m, KAZUO SASAKI, MITSUYOSAI OMOTO, Shiga XASAMI IlEHARA Department of Dermatology, Unrv. Med. Science. Seta. Otsu. Japan. we ~revlously reported that acetone soluble human uander often produced positive patch test in patients with atoplc dermatitiscAD). In the present study, we ~unohistolcgically examined the positive patch test site. [XetidislTwelve patients with AD were examined.Biopsy spc-cimens were take" fran the positive patch test sites at 2 tc 7 days. The specimenswere stainedwith a panel uf monoclonal jntibodies(Leu-1,Leu-2a, Leu-3a, OKT-6, HLA-DR, axI Leu-14j. [Results]In all specimensexamined,HIA-DR positive epidermal cells were observed in patchy patter". De-1 inflltratfmostly consistedof T cells. The ratio of La-A3 positive cells to La-2a positive cells varied frcm Li.i to 7.7krlea" 6.0). LCcmurentl Positive patch test sites tu acftonc soluble human dander irmwnohistolugically resembles to A0 lesions.
231
Symposium
(51-512)
Jl THE EVALUATION
OF
HORMONE
IN PATIENT
NITH ATOPIC
DERJ1ATITJ.S KIYOSHI Department
KAGAMI of Dermatolosy,
Kyoto First Red Cross
Hospital At birth, sebaceous deveroped as a result ulation
and
glands of
are moderately
maternal
hormonal
well stim-
much reduced in childin general, the sebaceous aland
sequentially
hood. I" puberty, deverop strlkinqly and 1s activated by the hormonal stimulation. In patient with atopic dermatitis the deficiency-of skin surface-lipids produced by sebaceous glands are noticed. The lower activity of sebaceous glands are presumably caused by the deficiency of hormones,such as androqe", estrogen or proqesterone. Jn this paper the value of hormone was estimated in patlent with atopic dermatitis compared with healthy controls.
54
5.3 RELEASE OF MACROPHAGE ANAPHYLACTIC REACTIONS
CHEMOTACTIC ACTIVITY IN HUMAN SKIN.
LA'TE PHASE REACTION
IN
OF THE SKIN
TAMOTSU KANZAKI AND HARUMI HORI DPpartment of Oermatoloqy, Nagoya Medlcal School, Nagoya
OSAMU KORO, SATORU YAMADA, ISAMU KIMURA, YASUSHI SUGITA AND SHOSO YAMAMOTO Dept. of Dermatology, Hiroshima Univ. School of Med. Hiroshima
City
un>.vers1ty
(LPR) IS an lmmedlate type Late phase reaction reactlo" but appr
It is known that mononuclear leukocytes accumulate in inflammatory lesion of atopic dermatitis. Tn the present study to investigate the mechanism of the accumulation, macrophage chemotactic activity (MCA) was determined in cutaneous anaphylactic reaction in patients with atopic MCA was dermatitis using skin chamber technique. remarkably increased in the chamber fluid obtained from the site with antigen at the interval of 1 hr up to 4 hr while at the control site there was no remarkable increase of MCA. The result shows that cutaneous anaphylactic reaction may evoke macrophage accumulation at the inflammatory lesion in the patients with atopic dermatitis.
dtoplc
dermatltls.
56
J5 RN IMMUNOHISTOLOGICAL
ANALYSLS OF WHEAT ALLERGEN IN ATOPLC UEKUTITIS ANU PKEPARATION OF HYPOALLEKGENICWHEAT
STUDY OF PATCH TEST LESIONS PROVOKED BY ACETONE. SOLUBLE HUMAN DANDER IN PA'PlENTS WITH ATOPIC DERMATITIS
Z. IKEZAWA, M. AHANO, M. KAGEYAMA,M. OKATU, K. TAKAHXHI, C. SUGA, A. SUGIYAMA, H. KOHATU, H. N&&JIMA AND K. TUBAKI* Departmentof Dermatology,Yokohama City Uni.Schoolof Medicine, Y,lkohama.*AsahidenkaInstitute.Tokyo We have already succeeded in preparationof hypaallrrgenit rice for elimination diet of rice in the patirncs of severe atopic dermatitis(AD)with rice allergy. We also revealedthat there are partial cross-reactionsbetween rice and wheat .antigens,and that wheae allergy may play an impurtant role in developemeneof severe AD in some cases. In this paper. we fractionatedthe wheat antigen proteins to water-, 1M N&l-, 70% ethanol-, and 4M urea-solublefracrions, and assayed IgE antibody tifer against rnch fraction. i-heresult:;showed thae 1M N&l-suluble fractionmay be major allergenic component of wheat. Then ,.,e prepared hypoallrrgenic wheat by decompositionof the fraction,and examined its clinical effect.
HISASHI SUGIL,m, KAZUO SASAKI, MITSUYOSAI OMOTO, Shiga XASAMI IlEHARA Department of Dermatology, Unrv. Med. Science. Seta. Otsu. Japan. we ~revlously reported that acetone soluble human uander often produced positive patch test in patients with atoplc dermatitiscAD). In the present study, we ~unohistolcgically examined the positive patch test site. [XetidislTwelve patients with AD were examined.Biopsy spc-cimens were take" fran the positive patch test sites at 2 tc 7 days. The specimenswere stainedwith a panel uf monoclonal jntibodies(Leu-1,Leu-2a, Leu-3a, OKT-6, HLA-DR, axI Leu-14j. [Results]In all specimensexamined,HIA-DR positive epidermal cells were observed in patchy patter". De-1 inflltratfmostly consistedof T cells. The ratio of La-A3 positive cells to La-2a positive cells varied frcm Li.i to 7.7krlea" 6.0). LCcmurentl Positive patch test sites tu acftonc soluble human dander irmwnohistolugically resembles to A0 lesions.
231