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The Initial Experience with the Implementation of the Bethesda System for Reporting Thyroid Cytopathology (BSRTC) in Our Institution
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Abstracts unlike traditional microscopy requires no slide preparation. Further work is needed to determine the feasibility of using OCT devices (e.g. probes) to image body fluids (e.g. effusions, ascites) in vivo for diagnostic use.
Figure 1
Figure 3
Cell nuclear dry mass histogram.
Pelvic washing with serous carcinoma
THYROID 152 The Initial Experience with the Implementation of the Bethesda System for Reporting Thyroid Cytopathology (BSRTC) in Our Institution Lu Wang, MD, Güliz Barkan, MD, Eva Wojcik, MD, MIAC, Stefan Pambuccian, MD Loyola University Medical Center, Maywood, Illinois
Figure 4
Mean cell nuclear dry mass.
151 Feasibility of Optical Coherence Tomography (OCT) for Pelvic Washings Liron Pantanowitz, MD, Muhammad Syed, MD, Jeffrey Fine, MD University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania Introduction: Optical coherence tomography (OCT) is a novel imaging technique that can reveal cellular architecture with micron-level resolution. OCT has been used instead of traditional microscopy to image tissues. There has been very limited use of OCT in cytopathology. Our aim was to determine the feasibility of OCT for imaging cytology samples. Materials and Methods: Seven archival pelvic washing specimens fixed in Cytolyt were centrifuged. Fluid drops from these specimens placed on a glass coverslip were imaged using OCT (LightCT, LLTech SAS, Paris France). OCT images were compared to ThinPrep and cytospin glass slides. Results: Using OCT, cells suspended within fluid could be detected. Malignant clusters of tumor cells were easy to visualize (Figure 1 shows a pelvic washing with metastatic serous carcinoma comparing an OCT image on the left to a Pap-stained cytospin specimen on the right). Nuclear detail was lacking. OCT image acquisition and imaging artifacts hampered good quality images. Conclusions: This investigation shows that cells present in cytology samples can be imaged using OCT. OCT is quick, non-destructive, and
Introduction: The implementation of the BSRTC has resulted in variable increases in Atypia of Undetermined Significance/Follicular Lesion of Undetermined Significance (AUS/FLUS), frequently to the rates above those recommended by BSRTC (7%). The initial published experience from different institutions suggests that “atypia” may be diagnosed in cases previously diagnosed as “unsatisfatory/nondiagnostic”, “benign” or “suspicious”. The aim of our study was to review our experience with the implementation of the BSRTC and determine the changes in the rates of reporting thyroid cytology brought by this system. Material and Methods: All thyroid fine needle aspiration FNA cases collected from 01/01/2006 to 9/25/2014 were identified through a search of our institution’s electronic records. Diagnoses made before and after the implementation of BSRTC at our institution (1/1/2011) were compared using the two-tailed Fisher exact test. Results: A total of 7341 thyroid FNA diagnoses was reviewed, including 3589 pre-BRSTC cases and 3742 post-BSRTC cases. Among all the diagnostic categories, AUS/FLUS rate has increased from 0.08% (preBRSTC) to 4.40% (post-BRSTC) (p<0.0001). At the same time, “benign” category dropped from 89.58% to 86.39% (p<0.0001). The changes of other diagnostic categories were not statistically significant.(As seen in Table 1) Conclusion: In contrast to other institution, we experienced only a modest increase in the use of the “atypia” category of reporting thyroid cytology. The concomitant decrease in “benign” diagnoses suggests the increase in “atypia” rate is made at the expense of the “benign” category.
Table 1
Abstracts unlike traditional microscopy requires no slide preparation. Further work is needed to determine the feasibility of using OCT devices (e.g. probes) to image body fluids (e.g. effusions, ascites) in vivo for diagnostic use.
Figure 1
Figure 3
Cell nuclear dry mass histogram.
Pelvic washing with serous carcinoma
THYROID 152 The Initial Experience with the Implementation of the Bethesda System for Reporting Thyroid Cytopathology (BSRTC) in Our Institution Lu Wang, MD, Güliz Barkan, MD, Eva Wojcik, MD, MIAC, Stefan Pambuccian, MD Loyola University Medical Center, Maywood, Illinois
Figure 4
Mean cell nuclear dry mass.
151 Feasibility of Optical Coherence Tomography (OCT) for Pelvic Washings Liron Pantanowitz, MD, Muhammad Syed, MD, Jeffrey Fine, MD University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania Introduction: Optical coherence tomography (OCT) is a novel imaging technique that can reveal cellular architecture with micron-level resolution. OCT has been used instead of traditional microscopy to image tissues. There has been very limited use of OCT in cytopathology. Our aim was to determine the feasibility of OCT for imaging cytology samples. Materials and Methods: Seven archival pelvic washing specimens fixed in Cytolyt were centrifuged. Fluid drops from these specimens placed on a glass coverslip were imaged using OCT (LightCT, LLTech SAS, Paris France). OCT images were compared to ThinPrep and cytospin glass slides. Results: Using OCT, cells suspended within fluid could be detected. Malignant clusters of tumor cells were easy to visualize (Figure 1 shows a pelvic washing with metastatic serous carcinoma comparing an OCT image on the left to a Pap-stained cytospin specimen on the right). Nuclear detail was lacking. OCT image acquisition and imaging artifacts hampered good quality images. Conclusions: This investigation shows that cells present in cytology samples can be imaged using OCT. OCT is quick, non-destructive, and
Introduction: The implementation of the BSRTC has resulted in variable increases in Atypia of Undetermined Significance/Follicular Lesion of Undetermined Significance (AUS/FLUS), frequently to the rates above those recommended by BSRTC (7%). The initial published experience from different institutions suggests that “atypia” may be diagnosed in cases previously diagnosed as “unsatisfatory/nondiagnostic”, “benign” or “suspicious”. The aim of our study was to review our experience with the implementation of the BSRTC and determine the changes in the rates of reporting thyroid cytology brought by this system. Material and Methods: All thyroid fine needle aspiration FNA cases collected from 01/01/2006 to 9/25/2014 were identified through a search of our institution’s electronic records. Diagnoses made before and after the implementation of BSRTC at our institution (1/1/2011) were compared using the two-tailed Fisher exact test. Results: A total of 7341 thyroid FNA diagnoses was reviewed, including 3589 pre-BRSTC cases and 3742 post-BSRTC cases. Among all the diagnostic categories, AUS/FLUS rate has increased from 0.08% (preBRSTC) to 4.40% (post-BRSTC) (p<0.0001). At the same time, “benign” category dropped from 89.58% to 86.39% (p<0.0001). The changes of other diagnostic categories were not statistically significant.(As seen in Table 1) Conclusion: In contrast to other institution, we experienced only a modest increase in the use of the “atypia” category of reporting thyroid cytology. The concomitant decrease in “benign” diagnoses suggests the increase in “atypia” rate is made at the expense of the “benign” category.
Table 1