Thursday posters

58s

49ti ASMS Conference THURSDAY

POSTERS

Thursday posters will be set up 7:30 - 8:00 am on Thursday morning. Presenters of odd numbered posters (001,003,005, etc) will attend their posters 8:45 - lo:15 am on Thursday. Presenters of even numbered posters (002, 004, 006, etc) will attend their posters 1:30 - 3:00 pm. Posters should be removed 7:30 - 8:OOpm on Thursday evening. INSTRUMENTATION,

001-023

ThPA 001 Improved Mass Accuracy in MALDI-TOF-MS Analysis

by Correction

for Systematic

Mass

Peptide Errors;

Johan Gobom; Volker Egelhofer; Martin Mueller; Hans Lehrach; E&hard Nordhoff; Max Planck Inst. for Molecular ThPA 002

ThPA 003

ThPA 004

ThPA005

ThPA 006

ThPA 007

ThPA 008

ThPA 009

Genetics, Berlin, Germany Vacuum Deposition Interface for Coupling Microbore Liquid Chromatography to MALDUTOF and TOF-TOF MS; Ping Hu; Tomas Rejtar; Jan Preisler; Franta Foret; Barry L. Karger; Northeastern University, Boston, M4 Performance of Vacuum Deposition Interface for Coupling of Capillary Electrophoresis to MALDI TOF/MS; Tomas Reitar; Ping Hu; Jan Preisler; Franta Foret; Barry L. Karger; Northeastern University, Boston, 44.4 Conversion Dynode for Enhanced High Mass Sensitivity in MALDI-TOF; Matthew S. Thompson; James P. Reilly; Indiana University, Bloomington, IN Optimizing the Extraction Voltage Waveform to Improve the TLF Focussing of Ions in Delayed Extraction MALDI; Robert S. Brown; Craig R. LaPerriere; Utah State University, Logan, UT TLC/MALDI/TOF/MS Using Particle Suspension Matrices; Anna Crecelius’; Malcolm R. Clench’; Don S. Richards*; Vie Par?; ‘Shejield Hallam University, Shefield, U.K; 2Pfizer Global R&D, Sana’wich, U.K; ‘SAZ Ltd, Manchester, U.K Pulsed Ion Extraction for MALDI-TOF with Constant Peak-Width over the Complete Mass Range; Armin E. m; Jochen Franzen; Bruker Daltonik GmbH, Bremen, Germany Transmission Geometry for Atmospheric Pressure Matrix-Assisted Laser Desorption Ionization; John H. Callahan’; Akos Verkes2; Marsha Galicia2; ‘Naval Research Laboratory, Washington, DC; 2George Washington University, Washington, DC A Novel Method for Interfacing Capillary Electrachromatography to Mass Spectrometry; David C

Simnson; Alexander J Yates; Patrick R R Langridge-Smith; University of Edinburgh, Edinburgh, UK Approach of TLC Coupling with Laser Desorption/ Ionization Time-of-Flight Mass Spectrometry; Yu-Chie Chen; Jia-Yi Wu; Inst. of Toxicology, Tzu Chi University, Hualien, Taiwan

ThPA 010 A Novel

ThPA 011 Atmospheric Pressure MALDI/Iou Trap Mass Spectrometry of Peptide-Peptide Interactions; Susanne C. Moyer’; Robert J. Cotter’; Amina S. Woods*; ‘Johns Hopkins University, Baltimore, MD; ‘NZDA, NZH, Baltimore, hrlD

ThPA 012 Collision Induced Dissociation (CID) of Singly Charged Tryptic Peptide Ions Produced by Electrospray and Atmospheric Pressure Matrix Assisted Laser Desorption Ionization (AP-MALDI) in an Ion Trap Mass Spectrometer (ITMS); D. 0. Konn’; P. Watts’; D. Despeyroux’; R. .J Cottef; S Moye?; ‘CBD Porton Down, Salisbury

UK; 2.fohns Hokins Universi@, Baltimore, MD MALDI-TOF MS with Thermal Field-Flow Fractionation (ThFFF) for the Analysis of Synthetic Polymers; Galina E. Kassalainen; S. Kim R. Williams; Colorado School of Mines, Golden, CO

ThPA 013 Coupling

on Mass Spectrometry

ThPA 014 How Much Data are Enough for Bacterial Identification?; Sharon T. Cebula; Kristin H. Jarman; Nancy B. Valentine; Catherine E. Petersen; Karen L. Wahl; Pa@

Northwest National Laboratory, Richland, WA Mass Accuracy for PSD Fragments in MALDI-ToF MS; Andrew R Bowdler’; Ian Brookhouse’; Emmanuel Raptakis’; Martin Resch*; ‘Kratos Analytical Ltd, Manchester, United Kingdom; 2Shimadzu Biotech, Duisburg, Germany ThPA 0 16 Prestructured MALDI Sample Supports: Sample Washing and Recrystallization; Martin Schuerenberq’;

ThPA 015 Improved

Peter Hutiagel’; Barbara Ehrhard’; Detlev Suckau’; E&hard NordhofPz; ‘Bruker Daltonik GmbH, Bremen, Germany; 2Ma-Planck-Znstitute for Mol. Genetics, Berlin, Germany of Injection Method for ROACHEMALDIIFTMS; Michele Stone; Jun Liu; Scott Collins; Rosemary Smith; Carlito Lebrilla; University of California, Davis, CA ThPA 018 Sulfur-Specific Detection of Impurities in Cimetidine Drug Substance Using Liquid Chromatography Coupled to High Resolution Inductively Coupled Plasma Mass Spectrometry and Electrospray Mass Spectrometry; E.

ThPA 017 Optimization

Hywel Evans’;

ThPA 019

ThPA 020

ThPA 021

ThPA 022

Jean-Claude

Wolf?;

Christine

Eckers’;

‘Plymouth Environmental Research Centre, Plymouth, UK; 2GlaxoSmithKline, Harlow, UK Measurement of Phosphorylation in Proteins by ICPMS; R. S. Houk; Yongjin Hou; Maria Pantelidou; Parag Chitnis; Ames Laboratory USDOE, Iowa State Univ., Ames, IA Flow Injection Analysis as a Means of Detecting and Overcoming Interferences in Inductively Coupled Plasma Time-of-Flight Mass Spectrometry; Denise M. McClenathan; Gary M. Hieftje; Dept. of Chemistry, Indiana University, Bloomington, IN Effects of an ICP-MS Sampling Interface and CeutralGas-Flow Rate on Analyte Number Densities in the ICP; Scott A. Lehn’; Mao Huang’; Kelly Wame?; Gerard0 Gamez’; Gary M. Hieftje’; ‘Dept. of Chemistry, Indiana University Bloomington, ZN; 2Dept. of Education, Harvard University, Boston, MA Figures of Merit for an ICP Mass Spectrograph; Ole A.

m; James H. Barnes IV; David A. Solyom; Gary M. Hieftje; Dept. of Chemistry Indiana University, Bloomington, IN

ThPA 023 Characterization

of the Fundamental Parameters of an ICP Source Exposed to a Mass Spectrometer Interface;

Gerard0 Gamez; Scott A. Lehn; Mao Huang; Gary M. Hiefije; Dept. of Chemistry, Zndiana University. Bloominpton. ZN ION MOLECULE

REACTIONS,

024 - 047

ThPB 024 Periodic Trends in the Reactivity of Atomic ions/Atomic ion-Benzene Adducts with 02; coina Caraiman; Gregory K. Koyanagi; Diethard K. Bohme; York University, Toronto, ON, Canada

ThPB 025 Studies of Spin-selective

Ring Cleavage of Aromatics by Transition Metal Cations Using an ICP/SIFT/CID Tandem Mass-Spectrometer; Voislav Blaeoievic; Gregory K. Koyanagi; Diethard K. Bohme; York University, Toronto, ON, Canada ThPB 026 Studies of the Gas-Phase Formation of Oxides, Nitrides and Sulfides of Atomic Ions Using an ICPlSIFTlCID Mass-Spectrometer; Vitali V. Lavrov; Gregory K. Koyanagi; Diethard K. Bohme; York University, Toronto, ON, Canada ThPB 027 Gas Phase Reactions of Transition Metal Ions with Disulfides: A Mechanistic and Structural Study by FT-

59s

49’ ASMS Conference on Mass Spectrometry ICR, Joseph T. Marini; David H. Russell; Texas A&M University, College Station, TX

ThPB 028 Analysis of Photochemlcal Reactions of Bis(l,lOphenanthroline)diamineruthenium(II) Complexes by Electrospray Ionization Mass Spectrometry; Rvuichi Arakawa; Kazuyuki Abe; Masayoshi Iwai; Tsuyoshi Fukuo; Yasuo Nakabayashi; Kansai University, Suita, Japan ThPB 029 Metal-Assisted Esterlficatlon of Dicarboxylic Acids with Transition Metal Ions in Electrospray Ionization Mass Spectrometry; Yang-111 Lee; Sung-Chart Jo; W. Andy Tao; R. Graham Cooks; Purdue University, West Lafayette, IN ThPB 030 Gas Phase Reactions of Binary Ag(I)/Substituted and Isotope Labeled Phenylalanine Complex Ions; Bupani A. Perera; Jessica M. Barr; Andrea L. Gallardo; Erach R. Talaty; Michael J. Van Stindonk, Wichita State University, Wichita, KS

ThPB 03 1 Using Ion-Molecule Reactions in a Quadrupole Ion Trap to Study the Coordination Structure of Transition Metal Complexes; Mariannv Y Combariza; Richard W Vachet; Universify of Massachusetts,

Amherst, MA

ThPB 032 Ligand Exchange and Bond Insertion in Reactions of (Di)Manganese Carbonyl Ions with Alcohols; Eric S. E. van Beelen; Steen Ingemann; University of Amsterdam, Amsterdam,

The Netherlands

ThPB 033 Methane C-H Bond Activation by Pt+; Peter B. Armentrout; Xiao-Guang Zhang; University of Utah, Salt Lake City, UT

ThPB 034 Reactions of Carbon Chain Anions with Interstellar Species; Veronica M. Bierbaum; Theodore P. Snow; Cynthia Barckholtz; Brian Eichelberger; University of Colorado, Boulder, CO

ThPB 035 Properties and Reactivity of Molecular Ions; Dominic Kearnev; Steve Price; University College London, London, UK

ThPB 036 Reaction Studies of Thermally labile, Nonvolatile Molecules Evaporated via Laser-induced Acoustic Desorption; Christopher J. Petzold; Luis E. RamirezArizmendi; Jenny L. Heidbrinlq Hilkka I. Kenttlimaa; Purdue University, W. Lafayette, IN

ThPB 037 Isotope Effect on Hydrogen Abstraction Reactions with Charged Phenyl Radical; Leonard P. Guler; Linhong Jing; Hilkka Kenttamaa; Purdue University, West Lafqette, IN ThPB 038 Intrinsic Chemical Properties of Nucleobases Evaporated Via Laser-Induced Acoustic Desorption; Ji-ang Liu; Ken Crawford; Christopher J. Petzold; Hilkka I. Kenttiimaa; Purdue University, W. Lafayette, IN

ThPB 039 Characterization of Drug-like Radical Intermediates in a FT/ICR Mass Spectrometer; F. Sedinam Ameaavibor; Chris J. Petucci; Hilkka I. Kenttiimaa; Purdue University, West Lafayette, IN

ThPB 040 Synthesis and Characterization of a Distonic Nitrene Ion: Gas-Phase Reactivity of Singlet and Triplet N-PhenyMNitrenopyridinium Ion; Shane E. Tichy’; Brian T. Hill*; JLarrv Camnbell’; Hilkka I. Kenttamaa’; ‘Dept. of Chemistry, Ohio State U., Columbus, Ohio; ‘Dept. of Chemistry, Purdue University, West Lafayette, IN

ThPB 041 Gas Phase Ion Chemistry of Alkenes; Michael E Lassman; Douglas P Ridge; Burnaby Munson; University of Delaware, Newark, DE

ThPB 042 Cyclizatlon of Acylium Ions with Nitrlles: Gas-Phase Synthesis and Characterization of 1,3,5-Oxadiazinium Ions; Marcos N. Eberlin; Luiz A. B. Moraes; Eduardo C. Meurer; State University of Campinas - UNICAMP, Campinas, SP, Brazil

ThPB 043 Reactions of Ketones with Gaseous Lewis Superacids; Philiu S. Maver’; Thomas H. Morton’; Danielle Leblanc’;

‘University of California, Riverside, Polytechnique, Palaiseau, France

Riverside,

CA; ‘Ecole

ThPB 044 Probing Reactive Intermediates by a Pulsed Nozzle and FTMS; A. L. Russell; D. C. Barbacci; D. Zhou; D. Read; P. P. Gaspar; M. L. Gross; Washington University, St. Louis, MO

ThPB 045 The Reactions of Oxide and Hydroxide with Diesters of Aliphatic Dicarboxylic Acids Using NICI and FT-ICR MS; Elizabeth A. Stemmler’; Colin Segovis’; Touradj Solouki2; ‘Bowdoin College, Brunswick, ME; *University of Maine, Orano, ME

ThPB 046 Selective Self - Ion / Molecule Reactions in Ion Trap Mass Spectrometer; Hui-Fen Wu; Ming-Yi Ho; ChienHung Chen; Chemistry Department, Tamkang University, Taipei Hsien, Taiwan

of Trimethyl Borate with ThPB 047 Gas-Phase Reactions Neurotransmitters and their Metabolites; Luis E. Ramos; Ana M. Femandes; Antonio J. Ferrer-Correia; Chemistry Department-University

Aveiro, Aveiro, Portugal

ION STRUCTURE & ENERGETICS, 048 - 064 ThPC 048 Determination of Alkali Metal Selectlvities of Dibenzo16-Crown-5 Lariat Ethers with Ether Pendant Groups Using ESI-MS; Sheldon M. Williams; Sheryl M. Blair; Jennifer S. Brodbelt; University of Texas at Austin, Austin, TX

ThPC 049 Metal Complexation of Novel Thio-Crown Ether Macrocycles by ESI-MS; Sheldon M. Williams; Wendi M. David; Jennifer S. Brodbelt; University of Texas at Austin, Austin, TX

ThPC 050 Opening Molecular Containers in the Gas Phase: Reactions of Cucurbituril Inclusion Complexes with Crown Ethers; David V. Dearden; Katherine A. Kellersberger; Brigham Young University, Provo, UT ThPC 051 Splitting of FTICR Peaks for Cyclodextrin Complexes: Evidence for Multiple Conformers?; Katherine A. Kellersberaer; David V. Dearden; Brigham Young University, Provo, UT

ThPC 052 Theoretical Calculations Applied to Problems in Negative Ion Thermochemistry; John E. Bartmess; Dept. of Chemistry, Univ. of Tennessee, Knoxville, TN

ThPC 053 The Salvation of I-HO+ by Argon, an Extension of an MP2 Study Using Density Functional Theory; Kellv 0. Sullivan; Creighton Universi& Omaha, NE ThPC 054 Determination of Gas-Phase Basic@ of Species Carrying Permanent Charge; Zoltan Takats; Laura M. Conner; R. Graham Cooks: Purdue Universitv. West Lafavette. IN ThPC 055 High-Resolution Pulsed Field ‘Ionization ‘Photoelectron Photoion Coincidence Study of NH, (NDs): Accurate Thermochemistry of NH,+ (ND,+) Systems; Yang Song’; Ximei Qian’; Weuwu Chen’; Jianbo Liu’; Cheuk-Yiu Ng’; ‘Iowa State University & Ames Laboratory, Ames, *Lawrence Berkeley National Laboratory, Berkeley, CA

IA;

ThPC 056 Radiatively Induced Dissociation of Water-Centered Cluster Cations Studied by FT-ICR; Eugene M. Markin; Terrance B. McMahon; University of Waterloo, Waterloo, ON

ThPC 057 On the Competitive Channels of Methyl Radical Loss with Methanol Loss from the Molecular Ion of Methyl (2Methoxymethyl-6-methyl) Benzoate in Metastable and Collision-Induced Dissociation; Hiroshi Yamaoka’; Roe1 H. Fokken?; Nice M. M. Nibbern& ‘Osaka Women‘s University, Faculty of Sci, Sakai, Osaka, Japan; *Universiiy of Amsterdam, Amsterdam, The Netherlands

ThPC 058 Triradical Thermochemistry from Collision-Induced Dissociation Threshold Energy Measurments; Loubna Harnmad; Paul Wenthold; Purdue University, West Lafqette,

IN

60s

49’h ASMS Conference

ThPC 059 The Gas-Phase Heat of Formation of Chloro-Substituted metalenzyne; Harvey A. Lardin; Paul G. Wenthold; Purdue University, West Lafayette, IN

ThPC 060 ICR Spectrometric Determination of Proton Affinities of Liquid Crystal Model Compounds; Daniel Ktihne; Karl Peter Wanczek; University Inst. Inorg. & Phys. Chem., Bremen, Germany

ThPC 061 The Proton Affinities of Two Weakly Bidentate Molecules:1,2-Dimethoxyethane and Methoxyacetone; Natalia Morlender-Vais; John L. Holmes; University of Ottawa, Ottawa, ON, Canada

ThPC 062 Laser Ion Kinetics : Direct Measurements of the Rearrangement Dynamics of Intramolecular Ionic Processes in Substituted Benzalacetones; Carsten Gnm; Tassilo Muskat; Juergen Grotemeyer; Inst. Phys. Chem., Universitat Kiel, Kiel, Germany

ThPC 063 Gas Phase Reactions of some Ionized Carbenes with Cyclopropane; Henri E. Audier; Philinne Mourgues; DCMR, CNRS UMR 7651 Ecole Polytechnique, France

Palaiseau,

ThPC 064 Silicon Versus Carbon Containing Ions; Henri E. Audier; Danielle Leblanc; Jacques Fossey; Jean-Pierre Denhez; DCMR CNRS UMR 7651, Ecole Polytechnique, France

Palaiseau,

GLYCOPROTEINS, 065 - OS5 ThPD 065 Peptide Mass Fingerprint of the Chicory I-FEH II Enzymes; Structural Elucidation of Glycosylations; Stefan Clerens’; Wim Van den Ende’; Peter D Verhaert3; Frans Vandesande’; ‘Lab. Neuro-endocrinology, K. U.Leuven, Leuven, Belgium; 2Lab. Developmental Biology, K. U.Leuven, Leuven, Belgium; ‘Organon NV, Oss, Netherlands

ThPD 066 Glycosylation Site ‘Analysis of Whole Protein Ions by Ion Trap MS/MS and Ion/Ion Reactions; Gavin E. Reid’; Min He’; Dawn Watson’; James L. Stephenson, J?; Scott A. McLuckey’; ‘Purdue University. Dept. of Chemistry West Lafayette, Indiana; 20ak Ridge National Laboratory Ridge, TN

Oak

ThPD 067 Determination of Protein Glycosylation in Proteomics; David Oxley; Niclas Karlsson; David Bishop; Nicole Wilson; Kate Cooper; Judith Webster; Nicki Packer; Proteome Systems Ltd, Sydney, Australia

ThPD 068 MALDUTOF MS Characterization of the N-Linked Oligosaccharides Derived from a Mouse Vomeronasal Protein; Yunnine. Huang; Yehia Mechref; Weidong Ma; Milos V. Novotny; Indiana University, Bloomington, IN ThPD 069 Characterization of Recombinant Proteins of Pharmaceutical Interest by Automated Peptide Mapping and On-line LC-MS; Zining Wei; David Dodsworth; Corey Knupp; Bristol-Myers Squibb, Hopewell, NJ ThPD 070 Comparative Analysis of Influenza Vaccines; Terrv D. Qr; Jean -C. Ethier; Michel Girard; Claude Dubuc; Laszlo Palkonyay; Sophie D’Aoust; Mary A. Hefford; Mario Dupuis; Biologics and Genetic Therapies Dir., Ottawa, Canada

ThPD 071 Characterization of the Isoform Heterogeneity of a Monoclonal Antibody by ES&MS; Peter Haunersberger; Ettore Ohage; Ute Stangner; Alexandra Stich; Olga Rovira; Ulrich Kohnert; SCIL Biomedicals GmbH, Martinsried, Germany

of ThPD 072 Characterization Glycosylation of Two Immunoglobulin M Monoclonal Antibodies by Mass Spectrometry; Fang Wang; Ruth H Angeletti; Antonio Nakouzi; Arturo Casadevall; Albert Einstein College of Medicine, Bronx, NY

on Mass Spectrometry

ThPD 073 Characterization of 0-Glycosylation Pattern of Glycoprotein X; Ken A. Lawson; Bemice Yeung; Jennifer Liu; Amgen, Inc., Thousand Oaks; CA l-hPD 074 Detection of Aberrant N-Glycosylation in CDG Syndrome by In-Gel Deglycosylation and MALDI-TOF MS Mapping; Diiana Sagi’; Thor&en Marquardt’; Jasna Peter-Katalinic’; ‘Inst. for Medical Physics and Biophysics, Miinster, Germany; ‘Clinic for Children Diseases, Miinster, Germany

ThPD 075 Characterization of N-linked Glycans of the Recombinant Endopolygalacturonase A from Aspergillus niger; Young Hwan Kim; Ron Orlando; Carl Bergmann; CCRC, The University of Georgia, Athens, GA

ThPD 076 Characterisation of Blood Coagulation Glycoproteins by Means of Gel Electrophoresis, High Resolution UV MALDI Mass Spectrometry and Seamless PSD Fragment Ion Analysis; Omar Beluacem’; Andrea Buchacher? Katharina Pock? Helen Montzomer?: Diuro Josic’; Andreas Rizzi’; Guenter Allmaier’y ‘Ociapha;ma Pharmazeutika, Vienna, Austria; ‘Kratos Analytical, Manchester, UK; 31nstitute for Analytical, Vienna, Austria

ThPD 077 Identification of Phosphorylation and Glycosylaiion Sites in Post-Translationally Modified Proteins; Alexandra M Furtos-Matei’; Jan Kihlberg2; Pierre Thibault’; ‘National Research Council of Canada, Ottawa, ON, Canada: 2Umea University, Umea, Sweden

ThPD 078 Glycosylation Analysis of Factor IX, Mava Belghazi’; Jean-Marie Schmitter’; Emmanuel Non?; Sami Chtourou2; Nicolas Bihoreau2; ‘Institut Europeen de Chimie Biologie, Pessac, France; 2LFB, Les Ulis, France

ThPD 079 Combining Reversed-Phase HPLC, High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection and MALDI-TOF MS for the Systematic Screening of Proteins for Glycosylation; Sophie Haebel’; Nora Eckermamr’; Regine Baeke?; Martin Steup’; ‘University of Potsdam, IFZB, Germany: ‘University of Potsdam, Biochemistry Germany; Veterinary Diagnostics, Germany

3,?‘fate Lab.

of

ThPD 080 Characterization of a Proline-Rich Glycoprotein Purified from Human Saliva; Eric A. Berg; James Grogan; Joseph Keen; Catherine E. Costello; Boston University School of Medicine, Boston, MA

ThPD 081 Profiling Intact Recombinant Glycoproteins with a Hybrid Quadrupole Time-of-Flight (Q-TOP) Mass Spectrometer for Batch-to-Batch Mass Comparability and Potential Low-Mass Modifications; Jason C. Rouse; Susan E. Abbatiello; Daniel E. Haq; Lisa A. Marzilli; Jennifer F. Nemeth; Suman Rathore; Thomas J. Porter; Hubert A. Scoble; Genetics Institute, Andover, MA ThPD 082 Analysis of Basal Lamina Proteins by MALDI MS and Capillary LC ES1 MS/MS; Manimalha Balasubramani’; Sucai Dong’; Willi Halfter’; Mark E. Bie?; ‘University of Pittsburgh, Pittsburgh, Pittsburgh, PA

PA; ‘Carnegie

Mellon

University,

ThPD 083 Glycoproteomics-Elucidation of the Structural Basis for Glycoprotein Heterogeneity Separated by 2-D Gel Electrophoresis; Manfred Nimtz; Eckart Grabenhorst; Harald S. Conradt; Gesellschaft Biotechn. Forschung, Braunschweig,

Germany

ThPD 084 Identification of GlcNAcylation Sites of Peptides and Proteins Using Q-TOF Mass Spectrometry; Robert J Chalkley’; A L Burlingame2; ‘Ludwig Institute for Cancer Research, London, Francisco, CA

UK; ‘Universify

of California,

San

ThPD 085 Characterization of Glycosylation Sites in the EGF Receptor; Yueiun Zhen; Richard M Caprioli; James V Staros; Vanderbilt University, Nashville, TN

49’ ASMS Conference on Mass Spectrometry PROTEINS: PHOSPHOPROTEINS. 086 - 112 ThPE 086 The Application of a Novel Precursor Ion Scanning Method for the Characterisation of Pbosphoproteins; James I. Langridge’; John B. Hoyes’; Alan Millar ; Ole N. Jensen2; Chris Jones’; Robert H. Bateman’; ‘Micromass UK Ltd. Manchester, England, UK; ‘University of Southern Denmark, Odense, Denmark Mapping of Native Net1 from ThPE 087 Phosphorylation Saccharomyces Cerevisiae; Susan L. Chen’; Michael J. Huddleston’; Wenying Shou’; Raymond Deshaies2; Roland S. Annan’; Steven A. Can’; ‘GlaxoSmithKline, King of Prussia, PA: ‘California Institute of Technology, Pasadena, CA ThPE 088 Repid Detection of Phosphopeptide Candidates from Protein Digests by Positive and Negative Ion MALDITOF MS; Yuiiauz Ma; Yun Lu; Wenjun MO; Thomas A. Neubert; New York University Medical Center, New York, NY of Multisite Phosphorylation in ThPE 089 Characterization Translation Initiator Factor 5 (eIF-5); Haitena Denp; Ruth H. Angeletti; Amitabha Bandyopadhyay; Romit Majumdar; Umadas Mama; Albert Einstein College of Medicine, Bronx, NY ThPE 090 Protein Phosphorylation Studied by Combination of IMAC and Data-dependent Acquisition of nanoES1 QTOF Product Ion Spectra; Andreas Schlosser; Dirk Bossemeyer; Wolf D. Lehmann; German Cancer Research Center (DKFZ), Heidelberg, Germany ThPE 09 1 Analysis of Phosphoproteins Captured by Antibodies to Phosphoamino Acids Using New Ion Trap MS/MS Methods; Rang-Fang Shen; Yvonne Lu; Hua Lin; Paul Shieh; Chris Becker; ThermoFinnigan Corp., San Jose, CA ThPE 092 On-Line Alkaline cRP-HPLC-Skimmer-CID-ESI-MassSpectrometric (A&ID-ESI-MS) Screening for Phosphopeptides; Alexander Beck’; Klaus Moeschel’; Martin Dee2; E&hart K. Schmidt’; Erwin D. Schleicher’; Rainer Lehmann'; ‘Clin. -them. Central Laboratory, Tuebingen, Germany; 21nterdisciplinarv Clin. Research Center, Tuebingen, Germany ThPE 093 On-probe AfBnity Extraction of Phosphopeptides Using Plasma Polymer Immobilized Metal Ions; Jim Zhang; Jason Whittle; Haibo Qiu; Richard B. Timmons; Gary R. Kinsel; University of Texas at Arlington, Arlington, TX of ThPE 094 Identification Physiologically Relevant Phosphorylation Sites in Myocyte Enhancer Factor 2 Using MALDI-TOF; D. M. Cox’; C. C. Yang’; K. W. M. Siu’; J. C. McDermott’; ‘Department of Biology, York University Toronto, ON, Canada; ‘Department of Chemistry, York University Toronto, ON, Canada ThPE 095 Negative Ion Dissociation of Phosphopeptides under MALDVTOF and EWFTICR Conditions; Suma Kaveti; Carolyn J. Cassady; Michael Peterson; Universitv of Alabama, Tuscaloosa, AL ThPE 096 Differential Protein Phosphorylation Profiling Using a Multidimensional Mass Spectrometry Method; Michael J. Huddleston; Francesca Zappacosta; Susan L. Chen; Steven A. Carr; Roland S. Annan; GlaxoSmithKline, King of Prussia, PA ThPE 097 High-confidence Phosphorylation Detection by MALDI Coupled with Site-specific stable Isotope Mass Tagging; Haininz Zhu; Li Yang; Thomas C. Hunter; Xian Chen; Los Alamos National Laboratory, Los Alamos, NM ThPE 098 Phosphopeptide Analysis by Positive- and Negative-Ion Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry; Katharina Janek ; Holger Wenschuh3; Michael Bienert’; Eberhard Krause’; ‘Institut fuer Molekulare Pharmakologie, Berlin, Germany; 21nstitutfuer

61s

ThPE 099

ThPE 100

ThPE 101

ThPE 102

ThPE 103

ThPE 104

ThPE 105

ThPE 106

ThPE 107

ThPE 108

ThPE 109

ThPE 110

ThPE 111

Biochemie, Charite, Berlin, Germany; 3Jerini Biotools GmbH, Berlin, Germany Investigation of Phosphoproteins Involved in Systemic Acquired Resistance Pathway in Arabidopsis. A Functional Proteomics Analysis; Helene Rogniaux’; Stefan Irmle?; Christian Pillone12; Daniel Hess’; Jan Hofsteenge’; ‘Friedrich Miescher Institute, Basel, Switzerland; 2Syngenta AG, Basel, Switzerland Affbtity Isolation and Tandem Mass Spectrometric Approaches for the Characterization of Phosphorylation within Mitogen Activated Protein Rinases; Michael J. Chalmers’; Walter Kolch’; Harald Mischak3; Simon J. Gaskell’; ‘UMIST, Manchester, UK; 2CRC Beatson Laboratories, Glasgow, UK; 3Department of Nephrologv, Hannover, Germany Affiity Purification of Phosphoproteins Using SiteSpecific Thiol Incorporation; Derek T. McLachlin; Brian T. Chait; The Rockefeller University, New York, NY Analysis of Phosphorylation by Automatic Multidimensional Liquid Chromatography-Electrospray Ionization Mass Spectrometry; Christer Ericson; Eric C. Peters; Ansgar Brock; David Horn; Jonathan Fitchett; Art Salomon; GNF (Novartis), San Diego, CA Selective Capture and Digestion of Phosphoproteins on Metal AfBnity Chromatography Beads; Alan A. Doucette; David R. Craft, Liang Li; University of Alberta, Edmonton, AB, Canada Modified Multidimensional Protein Identification Technology for Mapping Protein Phosphorylation in Complex Mixtures; Michael J. MacCoss; Dayin Lin; John R. Yates; The Scripps Research Institute, La Jolla, CA Phosphoprotein Analysis from Protein Mixtures by Nanoflow LCIMSIMS; Allan Stensballe; Ole N. Jensen; University of Southern DenmarWOdense, Odense, Denmark Rapid, Selective, and Sensitive Detection, Mapping and Sequencing of Phosphorylated Peptldes Using ESIFTICR-MS and IRMPD; Jason W. Flora; David C. Muddiman; Virginia Commonwealth University, Richmond VA Rapid Identification and Characterization of Posttranslational Modifications from y-Irradiated Human Skin Fibroblasts (HSF) Using Stable-Isotope Assisted Mass Spectrometry (SIAMS); Thomas C. Hunter’; Mary Jo Waltman’; Thomas W. Schattuck3; Peter M. Yau2; E. Morton Bradbury’; Xian Chen’; ‘Los Alamos National Lab, Los Alamos, NM; 2University of Cahfornia, Davis, CA; ‘Colby College, Waterville, ME Identification of Novel Phosphorylation Sites of the Hexon Protein in Adenovirus Type 5; Suzanne M. Ramirez’; Gary W. Ketner?; Robert J. Cotter’; ‘Johns Hopkins School of Medicine, Baltimore, MD; 2Johns Hopkins School of Public Health, Baltimore, MD Phosphorylation Degree of Phosphoproteins Analyzed by Combination of Capillary Liquid Chromatography and Element Mass Spectrometry; Mathias Wind; Horst Wesch; Wolf D. Lehmann; German Cancer Research Center, Heidelberg, Germany Analysis of Post-Translational Modifications Using a MALDI TOF-TOF Mass Spectrometer; Jon P. DeGnore; Jennifer Campbell; Peter Juhasz; Xiangping Zhu; Martin Hornshaw; Marvin Vestal; John Peltier; Applied Biosystems, Framingham, MA Quantitative Monitoring of Peptide Phosphorylation In Vitro and In viva Using Capillary Column LC-Tandem Mass Spectrometry; Cristian I. Ruse’; Belinda Willard’; Thomas Haas’; J.-P. Jin2; Meredith Bond’; Michael Kinter’;

62s ‘Cleveland Clinic Foundation, Cleveland, OH; ‘Case Western Reserve University, Cleveland, OH ThPE 112 Isotope Coded Reducible Affinity Phosphate Detection in Peptides Involving SerlThr (ICRAPDINPIST); Min Shen; Richard S Johnson; Alison Wallace; Immunex Corporation, Seattle, WA PROTEOMICS: NEW AND IMPROVED METHODS, 113 - 138 ThPF 113 High Throughput LC/MS and Data Processing for Library Characterization; Janshon Zhu; Kimpor Ng;-Triad Therapeutics, Inc., San Diego, CA ThPF 114 Identification of Proteins Using Nanoscale Electrospray from Basic pH Solutions; Paul C. Goodlev’; Hongfing Yin’; ‘Agilent Technologies, Palo Alto, CA; 2Agilent Technologies, Palo Alto, CA ThPF 115 Mass Spectrometry Driven BLAST Homology Searching (MS BLAST): Mass Spectrometry Meets Bioinformatics; Andrei Shevchenko’; Alexander Loboda’; Shamil Sunyaev4; Werner Ens3; Peer Bork4; Kenneth G. Standing3; ‘MPI Molecular Cell Biology and Genetics, Dresden, Germany; 2MDS Sciex, Toronto, ON, Canada; ‘University of Manitoba, Winnipeg, MB, Canada; 4EMBL, Heidelberg, Germany ThPF 116 Methods to Improve Peptide Recovery from In-Gel Digestions; Kathy L. O’Connell; David Arnott; John T. Stults: Genentech. Inc., S. San Francisc. CA ThPF 117 Solving Integral Membrane Protein Structures Using Chemical Cross-linking and High-resolution Mass Spectrometry; Malin M. Young; Camille B. White; Saul Datwyler; Joseph S. Schoeniger; Sandia National Laboratories, Livermore, CA ThPF 118 MALDI MS and Gel Electrophoresis Analysis of Low and High Mass Proteins from Rapid Solvent Extraction of Bacterial Cells; Jinx Zheng; Zhengping Wang; Liang Li; Universitry of Alberta, Edmonton, AL, Canada ThPF 119 N-terminal Peptide Sequencing by Q-TOF Tandem Mass Spectrometry and Accurate Mass Determination of LowMass b-Ions; Wolf D. Lehmarm; Andreas Schlosser; German Cancer Research Center (DKFZ), Heidelberg, Germany ThPF 120 Enzyme Activity Assay Using LC-MS: A pH Profile Study of Methionine Aminopeptidase; Stone D.-H. Shi’; Mark E. Hemling’, Mark F. Bean’, Steven A. Cad; Guang Yang’; Thaddeus A. Tomaszek’. IDept. CASS, GlaxoSmithKline, King of Prussia, PA; bept. Mech. Enzy., GlaxoSmithKline, King of Prussia, PA ThPF 121 Evaluation of MS/MS Methods for Determining Maximal Coverage of H. influenza Proteome; Eugene C. Yi’; Serge Stolyar’; Samuel Purvine’; Jimmy K. Eng ; Eugene Kolker’; Ruedi Aebersold’; Arnold L. Smith’; David R. Goodlett’; ‘The Institute for Systems Biology, Seattle, WA; 2University of Missouri, Columbia, MO ThPF 122 HPLCl ES1 I Mass Spectrometry Methods for Probing of Acetylated Lysine in Protein by Modification-Specif Marker Ions; Jin Youncr Kim’; Kyoung Wook Kim ; Kun Cho’; Ho Jeong Kwon’; Dai Woon Lee3; Jong Shin Yoo’; ‘Korea Basic Science Institute, Daejeon, Korea; 2Sejong University, Seoul, Korea; ‘Yonsei University, Seoul, Korea ThPF 123 A New Powerful Stain for the Detection of Protein in 2-D Gels with Minimal Interference with Mass Spectrometry Analysis; Jean-Marc Strub’; Thierry Rabilloud’; Nukhet Cavusoglu’; Sylvie Luche*; J. Lunardi*; Alain van Dorsselaer’; ‘ULP-LSMBO UMR CNRS 7509, Strasbourg, France; 2CEA-EA 2943 DBMS/BECPCEA, Grenoble, France ThPF 124 An Investigation of the Residue from the Pyrolysis of Proteins Using MALDI-TOF-MS; Mohammed A. Meetani; Kent J. Voorhees; Colorado School of Mines, Golden, CO

49” ASMS Conference on Mass Spectrometry ThPF 125 Rapid On-Probe Digestion of Bacteria for MALDI; William A. Harris; James P. Reilly; Dept. of Chemistry, Indiana University, Bloomington, IN ThPF 126 Protein Characterization and Identification by Capillary HPLC - Tandem Mass Spectrometry of Intact Proteins; Ping Du; Rachel R. Ogorzalek Loo; Joseph A. Loo; Pfizer Global Research and Development, Ann Arbor, MI ThPF 127 TransAtlas - A Novel Approach to Proteomics; Andy J. Tomlinson; Silvia S. Kwak, Wade M. Hines; Alexandru cl. Lazar; George Morris; Robert G. Urban; Roman M. Chicz; ZYCOS Inc., Lexington, MA ThPF 128 Proteomics Applications of Information-Dependent Data Acquisition on a Triple Quadrupole Mass Spectrometer; Chris L. Stumnf; Ying-Qing Yu; George M. Hilliard; U.C. College of Medicine, Cincinnati, OH ThPF 129 An integrated Approach to Identifying Proteome Samples; Peter Huthagel; Ulrike Schweiger-Hufhagel; Detlev Suckau; Andrea Schneider; Ania Resemann; Bruker Daltonik GmbH, Bremen, Germany ThPF 130 Free-Flow Electrophoresis and Mass Spectrometry Strategy for Proteomic Studies of Subcellular Compartments: A Tool for Functional Genomic; Muriel Boussac’; Clotilde The$; Sebastian Amigorena*; Jerome Garin’; ‘CEA, Grenoble, France; ‘INSERM US20 - Institut Curie, Paris, France ThPF 131 Post Source Decay Analysis of Large Peptides (1 2,500 Da) and its Value in Protein Identification; Chris W. Sutton’. Tom Hassell*; William K. Kappel*;‘Kratos w&al, Manchester, UK; ‘Sigma-Aldrich, St. Louis, MO ThPF 132 Sensitivity Enhancement for Proteomics Applications with an Improved Online NanoESI Source for an Ion Trap; Michael Schubert; Uhike Schweiger-Hufhagel; Carsten Baessmamr; Arnd Ingendoh; Catherine Stacev; Bruker Daltonik GmbH, Bremen, Germany ThPF 133 Use of an Acid-Labile Surfactant as SDS Substitute for Gel Electrophoresis and Digest Analysis; Martin Zeller’; Jasna Peter-Katalinic’; Edouard S. P. Bouvie3; Simone Koenig’; ‘University of Muenster, Muenster, Germany; ‘Waters Corp., Milford, MA ThPF 134 Mass Spectrometric Characterization of CysteineContaining Proteins; Fabio Talamo; Tiziana Anelli; Massimo Alessio; Roberto Sitia; Angela Bachi; Dibit-San Raffaele Scienttfic Institute, Milan, Italy ThPF 135 Recovery of Intact Proteins from Gel Separations for Characterisation by Mass Spectrometry; Andreas P. Jonsson; Carina Palmberg; Tomas Bergman; Karolinska Institutet, Stockholm, Sweden ThPF 136 Improved Mass Spectral Accuracy for Peptide Fingerprinting; Jan Axelsson; Amersham Pharmacia Biotech, Uppsala, Sweden ThPF 137 Silver Stain Removal Using H202 for Enhanced Peptide Mass Mapping by MALDI-TOF-MS; Llovd W. Sumner’; Steve White2; Barbara Wolf-Sumner’; Victor S. Asirvatham’; ‘The Samuel Roberts Noble Foundation, Ardmore, OK; 20klahoma State University Stillwater, OK ThPF 138 On-Chip Limited Proteolysis and Protein Identification Using Non-Specific Proteases and ProteinChip@ Array LDI Qq-TOF MS technology; Ninu Tang’; Pete Tomatore’; Alan J. Smith2; Scot R. Weinberger’; ‘Ciphergen Biosystems, Fremont, CA; 2Stanford University, Palo Alto, CA PROTEOMICS:

LABELING, QUANTITATION & AFFINITY TECHNIQUES, 139 - 170 ThPG 139 Global Internal Standard Technology for Quantifying Protein in Proteomics; Fred E. Reznier; Larry D. Riggs; Cathy S. Sioma; Junyan Ji; Shihong Wang; Asish

63s

49’ ASMS Conference on Mass Spectrometry Chakraborty; Xiang Zhang; Purdue University, West Lafayette, IN ThPG 140 The Use of Isotope-Coded Affhdty Tags to Measure in Deinoccocus Abundances Relative Protein radiodurans; Thomas P. Conrads; Timothy D. Veenstra; Nicolas H. Anaell; Ellen A. Panisko; David J. Anderson; Gordon A. Anderson; Oleg V. Borisov; Yufeng Shen; Ljiljana Pasa-Tolic; Nicola Tolic; Riu Zhao; Richard D. Smith; Pacific Northwest National Laboratories, Richland, WA

ThPG 141 Analysis Liquid

of ICAT Labelled Proteins via Automated Chromatography/Orthogonal MALDI Qq-TOF;

Chris M Lock’; Tim Griffin*; Ruedi Aebersold*; ‘MDSSCIEX, Concord, Canada; 21nstitute for Systems Biology Seattle, WA ThPG 142 ICAT MS Analysis of Arabidopsis Thaliana; L,J&

ThPG 152 An Isotope Encoded Method for Accurate Quantitation of Protein Phosphorylation; Xiaolong Zhang; Qian K. Jin; Steven A. Carr; Roland S. Amian; GlaxoSmithKline, King of Prussia, PA

ThPG 153 An Isotopic Label for the Differential

Quantitation of A New Use of an Old Friend; Eric C. Peters; Christer Ericson; Ansgar Brock; GNF (Novartis), San Diego, CA ThPG 154 A New Chemical Method for Simplifying Complex Mixtures of Peptides for Proteomic Analysis by LCNWMS; Thomas A. Shaler; Christopher H. Becker; ThermoFinnigan Corp., San Jose, CA ThPG 155 Differential Isotopic Labeling of Peptides for Global Quantification of Proteins and De Novo Sequence Derivation; David R. Goodlett; Andrew Keller; Samuel Protein Expression:

Purvine; Eugene C. Yi; Julian Watts; Ruedi Aebersold; Priska von Haller; Richard Newitt; Eugene Kolker; The

Nuwavsir; Tina A. Settineri; Christie Hunter; Alpesh Patek Daniel Schaeffer; Applied Biosystems, Foster City, CA ThPG 143 Quantitation

and Identification of Proteins Using Isotope Coded Affinity Tags: A Comparison of MALDI-TOF and ESI-TOF Mass Spectrometry; John M. Peltier; Sofia G.

Institute for Systems Biology, Seattle, WA Mass Spectrometry Fragmentation and Identification of Cysteine-Modified Peptides; Oleu V.

ThPG 156 Tandem

Borisov Michael B. Goshe; Thomas P. Conrads; Ellen A. -2 Panisko; Timothy D. Veenstra; Richard D. Smith; Pacific

Hohnholt; Xiangping Zhu; Jon DeGnore; Edward J. Takach; Applied Biosystems, Framingham, MA Protein Expression Changes in the Liver Of Phenobarbitol Treated Rat with Isotope-Coded Affinity Tags (ICATTM) and Nanoscale Capillary LCMSIMS;

ThPG 144 Mining

Roderick G. Davis’; Arthur Moseley’; David D. Taylor’; Jim ‘GlaxoSmithKline, RTP, NC; ‘Applied Langridge’; Biosystems, Cambridge, MA ThPG 145 Quantitative Mitochondrial Proteome Analysis Using Isotope Coded Affhrity Tags; Swathi Arm’; Uche E. Uche’; Abiola Oladipo’; Jimmy Eng’; David Han’; ‘University of Connecticut Health Center, Farmington, CT; 21nstitute for Systems Biology, Seattle, WA ThPG 146 The Mammalian Mitochondrial Ribosome: Towards Identification of the Full Complement of Rlbosomal Proteins Present; William A. Burkhart’; Robert K.

Blackbum’; Emine C. Koc’; M. Arthur Moseley’; Mary B. Moyer’; Linda L. Spremulli2; ‘Glaxo SmithKline, RTP, NC; 2University of North Carolina, Chapel Hill, NC ThPG 147 Quantitative Membrane Proteome Profdes of Apoptotic Cell Using Isotope-Coded Affinity Tags (ICAT); Swathi

Arm’; Abiola Oladipo’; Uche E. Uche’; Jimmy Eng’; m Han’. I University of Connecticut Health Center, Farmington, Connecticut; 21nstitute for Systems Biology, Seattle, WA ThPG 148 Targeted Delta-Mass Data Dependent Tandem MS of Protein ICAT-Labeled Peptides to Determine Expression; Tina A. Hemenway’; Eugene C. Yi’; David R. Goodlett’; ‘ThermoFinnigan, San Jose, CA; 2The Institute for Systems Biology, Seattle, WA ThPG 149 Isotope Coded Reducible Affinity Proteomics (ICRAP);

Richard S Johnson; Alison Wallace; Lin Guo; Min Shen; Immunex Corporation, Seattle, WA Labeling of Phosphoserine/Threonine on Proteins as a Tool for Probing the Phospho roteome; Yoshiva Oda’; Takeshi Nagasu’; Brian T Chait’; P.Elsal . Co., Ltd,, Tsukuba, Japan; 2The Rockefeller University, New York, NY ThPG 151 Phosphoprotein Isotope-Coded Affinity Tag (PhIAT): Application to the Enrichment, Quantitation, and Identification of Phosphoproteins Using LC-MS and LCMS/MS; Michael B. Goshe; Thomas P. Comads; Ellen A.

ThPG 150 Selective

Panisko; Nicolas H. Angell; Timothy D. Veenstra; Richard D. Smith; Pacific Northwest National Laboratory Richland, WA

Northwest National Laboratory, Richland, WA of Protein Components in Open and Closed Eye Tears; Kim Funq’; Carol Morris’; Robert Sack3; Mark Duncan’; ‘UCHSC, Denver, CO; 2Ciba Vision, Duluth, GA; ‘SUNY; Manhattan, NY Comparative Proteomics: Proteolytic Labeling and Online Capillary LC-MS/MS Characterization; Kristy J. Revnolds; Xudong Yao; Catherine Fenselau; University of Maryland, College Park, MD Affinity Linked Amino Acid Reactive Mass Tags for Multiplexed Quantitative Analysis of Complex Protein Mixtures; Haihonp Zhou; Rajendra Singh; SurroMed Inc., Mountain View, CA Post-digestion Labeling with 180/160 for Rapid Sequence Analysis of Novel Proteins; Rong Li; Robert Z. Qi; Institute of Molecular and Cell Biology, Singapore Inverse 180 Labeling - Mass Spectrometry for the Rapid Identification of Marker / Target Proteins; Y. Karen

ThPG 157 Quantification

ThPG 158

ThPG 159

ThPG 160

ThPG 161

m;

Zhixiang Ma; Douglas F. Quinn; Emil W. Fu;

Novartis Pharmaceuticals Coqoration, Summit, NJ ThPG 162 Refit&$ High Throughput Peptide Analysis (Proteomics) Using 0 Labeling; Ian I. Stewart; Ty Thomson; Daniel Figeys; MDS Proteomics Inc, Toronto, ON, Canada ThPG 163 Relative Quantification of Trypic Digests of Proteins by MALDI MS Using Stable Isotope Labels; Stephanie

Bourin; Bengt Bjelqvist; Maria Liminga; Jean Luc Maloisel; Ronnie Palmgren; Amersham Pharmacia Biotech, Uppsala, Sweden

ThPG 164 Withdrawn ThPG 165 New Protein Using

Detection and Identification Strategies ProteinChip Array Technology; Ning Tang’;

Shanhua Lin’; Scot R. Weinberger’; Ron Orlando*; Dan King*; Al Smith3; I Czphergen Biosystems, Inc., Fremont, CA; 2Complex Carbonydrate Research Center, Athens, GA, ‘Beckman Center, Stanford University Palo Alto, CA ThPG 166 Specifity of Trypsin Cleavage after Lysine Modification Investigated by LC-MS/MS; Sonja Hess; Lewis K. Pannell; SMSF, LBC, NIDDK, NIH, Bethesda, MD ThPG 167 Development of ESI-MS and MALDI-QqTOF-MS Methods for the Analysis of PMP-Labelled N-Linked Oligosaccharides from Polyclonal IgG Standards: Comparison with HPAEC-PAD Analysis; Julian A. Saba’;

Jeremy P. Kunkel’; Maciej P. Bromirski’; Werner Ens*; Kenneth G. Standi&; James C. Jamieson’; Helene Perreault’; ‘Dept. of Chemistry, University of Manitoba,

64s

49” ASMS Conference on Mass Spectrometry Winnipeg, MB, Canada: 2Dept. of Physics, Manitoba, Winnipeg, MB, Canada

University

of

ThPG 168 Isotopically Labeled Reagents Allow Easy Recognition of Crosslinked Peptides; Kara M. Pearson*; Lewis K. Pannell’; Hemy M. Fales’; ‘NHLBIITVIH, Bethesda, MD: 2NIDDK7NIH, Bethesda, MD

ThPG 169 2D Kinetics of Biotin-Streptavidin Interactions by Bead Injection Electrospray Mass Spectrometry; Yuko Ozata; Frantisek Turecek; Michael H Gelb; Jaromir Ruzicka; University of Washigton, Seattle, WA

ThPH 182 On-Site MS Analysis of WW2 Cylinders and Barrels Filled with Unknown Liquid; Enrico Davoli’; Luigi Cappellini’; Massimo Bonfa”; ‘Mario Negri, Milano, Italy, ‘Ambiente S.p.A., Milano, Italy

ThPH 183 Indentification of Tank Waste Components Using Infrared MALDI; Michael R. Panantonakis’; David R. Ermers; Michelle L. Baltz-Knorr’; Wayne P. Hess3; Richard F. Haglund, Jr. ‘; I Vanderbilt University, Nashville, TN, 2Mississippi State University, Mississippi St., MS; 3Pact$c Northwest National Laborato y, Richland, WA

ThPG 170 Metabolite Profile of SU6668 in Human Urine by ESIHybrid Quadrupole Time of Flight Mass Spectrometry; Ari Gritsas’; Themis Flarakos’; Mark L. J. Reimer’; Lome M. Fell’; Eva Duchoslav’; Gillian Cropp3; ‘MDS Pharma

ThPH 184 Application of MALDUTOFMS for the Analysis of Regulatory DQO Organic Compounds; Eric W. Honne; Jeremy R. Lohman; James A. Campbell; Pact& Northwest

Services, Montreal, QC, Canada; ‘MDS Sciex, Toronto, ON, Canada; ‘Sugen Inc., San Francisco, CA

ThPH 185 Development of an Assay for the Quantitation of Trlphenylphosphine Oxide Using an Ion Trap Mass Spectrometer; Narasimhan Kasthurikrishnan’; Joshua Tilghman2; Vasu Sekhar’; ‘Pfter, Groton, CT; 2Lehigh

ENVIRONMENTAL, 171- 196 ThPH 17 1 Trace Analysis of PAHs in Fish Oil; Joseuh C. Pan; Sairam Abburu; Jason D. Herrera; Jean C. Pritchett; Kevin R. Villalobos; Southwest Research Institute, San Antonio, TX ThPH 172 SFE-plus-Cl8 Lipid Cleanup Method for GC-MS Quantitation of PAHs in Smoked Meat; Md. Yeakub Ali; Richard B. Cole; University of New Orleans, New Orleans, LA

ThPH 173 PAHs and Heavy Metals in New Orleans Soils and Sediments; Oiang Zhanq; Howard W. Mielke; Chris Gonzalez; Van Quach; Guangdi Wang; Xavier University of Louisiana, New Orleans, LA

ThPH 174 Rapid Trace Analysis by Coupling GC/TOF-MS to Narrow-Bore Columns Using Non-Splitting Injection Techniques; Nick P Bukowski; Lu Lin; Scott J Harrison; Therm0 Finnigan, Manchester,

UK

ThPH 175 Mass Spectral Imaging of Three Dimensional Particles: Ultraviolet Two-Step Laser Mass Spectroscopy (UVL*MS) Imaging of Polyaromatic Hydrocarbons (PAH) Distributions on Creosote Contaminated Soil Pareticles; James L. Fve; John H. Callahan; Herbert H. Nelson; Andrew P. Baronavski; Parmely H. Pritchard, Naval Research Laboratory

Washington, DC

ThPH 176 Analysis of Polyaromatic Hydrocarbons by Atmospheric Pressure Photoionixation LC/MS; Patricia H. Cormia; Steven M. Fischer; Christine A. Miller; Agilent

University, Bethlehem, PA

ThPH 186 GClMS Characterization of Natural Hydrocarbon Release from Oil Sands Deposits in Tributaries of the Athabasca River Basin, Canada; John V. Headley’; Christine Akre’; Kerry M. Peru’; Malcolm Conl$; Leslie C. Dickson3; ‘National Water Research Institute, SK, Canada; 2Canadian Wildlge Services, SK, Canada: 3Canadian Food Inspection Agency, SK, Canada

ThPH 187 Daughter Ion Spectra of Hydrolyzed and Oxidized Sulfur Mustards; Paula S. Vanninen; Heikki Bjisrk; VERIFIN, Helsinki, Finland

ThPH 188 Characterization of Chemical Warfare Exposure Using Static SIMS; Garv S. Groenewold’; Anthony D. Appelhans’; John E. Olson’; Gary L. Gresham’; B. Rowland’; M. Jeffreg; ‘Dugway Proving Ground, Dugway, UT; ‘INEEL, Idaho Falls, ID

ThPH 189 MS/MS of Sarin-de Methylphosphonate; Michael

Canada

ThPH 179 Automated Ultra-Trace Analysis of Toxic Pollutants in Environmental Samples by Large Volume Injection (LVI) Technique GCIMS; Albino Sironi’; Faust0 Munari’; Christian Soulie?; Marc Thermonia3; ‘Therm0 Finnigan, Paris, France;

‘DCMS,

ThPH 180 Potential Interference of PCBs in Determination of PBDEs in Environmental Samples Using GC/MS and GCIHRMS; Mehran Alaee; Sean Backus; Christina Cannon; National Water Research Institute, Burlington, ON, Canada

ThPH 181 Methane CI and Electron Impact LCMS Analysis of Environmental Monitoring Site Discovery; Scott Scott’; Bill Said’; ‘CSS Analytical Co. Inc., Shawnee, KS; ‘Analytical Management Laboratories Inc., Olathe, KS

and Diisopropyl-drr D. Crenshaw; Battelle

Memorial Institute, Columbus, OH

ThPH 190 Development of a Packed Capillary LC-ES&MS Method for the Analysis of Organophosphorus Chemical Warfare Agents, their Hydrolysis Products and Related Compounds in Aqueous Samples; Paul A. D’APostino; James R. Hancock; Claude L. Chenier; Defence Research Establishment

Technologies, Inc., Palo Alto, CA

ThPH 177 Petroleum/Water Drilling Mud and Large IR Gas Cells in ID of Hydrolysis Products by GC/IMS; Ronnv C. Robbins; Peter A. Snyder; Ashish Tripathi; Augustin S. Ifarraguerri; US Army, APG, MD ThPH 178 The Use of SFC Combined with Mass Spectrometry in the Investigation of Nitro-PAHs Containing Samples; Genevieve Mercier; Louis Ramaley; Robert D. Guy; Michael A. Potvin; Dalhousie University, Halifax, NS,

Milan, Italy; 2Thermo Finnigan, Bierges, Belgium

National Laborato y, Richland, WA

Sufield,

Medicine Hat, AL, Canada

ThPH 191 Electrospray Ionization FT-ICR MS Analysis of Explosives; Zhizane. Wu’; Christopher L. Hendrickson’; Ryan P. Rodgers’; Alan G. Marshall’; ‘Florida State University, Tallahassee, FL; ‘National High Magnetic Field Laborato y, Tallahassee, FL

ThPH 192 Trace Detection and Quantitation of Explosives Using LC/APCI-MS; Richard F. Reich; Richard A. Yost; University of Florida, Gainesville, FL

ThPH 193 ICP MS: Analysis for Total Mercury in Fish Samples; Marc E Enzel; Stacie Hammack; FL Dept of Agric. and Consumer Services, TaIlahassee, FL

ThPH 194 Surface Characterization of Soil Exposed to Cs+; Brittanv D.M. Hodges’; Robert V. Fox’; Dorinda M. Funk’; Mamie M. Cortez’; Recep Avc?; Jani C. Ingram’; Gary S. Groenewold’; ’ INEEL, Idaho Falls, ID; ‘Montana State Universiv,

Bozeman, MT

ThPH 195 Quantitative Analysis of Environmental Pollutants with the Metastable Atom Bombardment (MAB) Source; Serge Moore’; Louis Vromet’; Anik Forest’; Denis Faubert2; Michel J. Bertrand3; ‘CEAEQ, Laval, QC, Canada; ‘DEPHY Technologies, Montreal, QC, Canada; Montreal, Montreal, QC, Canada

3University

of

ThPH 196 Comparison of a Magnetic Sector Mass Spectrometer with the MAB-TOfMS for Quantitative Analysis of

49’hASMS Conference on Mass Spectrometry

65s

Environmental Pollutants; Anik Forest’; Serge Moore*; Pascal Martin’; Louis Vromet*; Denis Faubert’; Michel J. Bertrand3; ‘DEPHY Technologies, Montreal, QC, Canada; 2CEAEQ, Laval, QC, Canada; Montreal, QC, Canada

‘University

Grenier; Michel Simard; Orthodoxia Fragiskatos; MDS ThPI 209

of Montreal,

DRUG METABOLISM, 197 - 235 High-Throughput LC-MS/MS Method for - _ Quantitative Analysis of Discovery Drug in Brain Homogenate; Xiao Ding; Jim Meyer; Krys Miller; Amgen, Inc., Thousand Oaks, CA High Throughput Optimization and ThPI 198 Automated Quantitation for LCNS and LCMSiMS; Shamim

ThPI 197 Automated

ThPI 210

ThP1211

Haider; Charles C Liu; Byron Kieser; Lyle Burton; Iryna Chervetsova; Yves LeBlanc; Applied Biosystems / MDS Sciex, Concord, ON, Canada of Parallel Columns and Extended Standard Calibration Curve in a Highly Efficient LC/MS/MS Method for the Quantification of V102862 and Its Acid Metabolite in Dog Plasma; Xinoinz Fang; Kevin Cook, Richard Badger; Ming Wang; Danlin Wu; Purdue Pharma L.P., Ardsley, NY High Throughput Sample Analysis in the GLP Environment; Thomas C. Alexander; Brian T. Hoffman;

ThPI 199 Application

ThPI 200

Kimberley ThPI 201

ThPI 202

ThPI 203

ThPI 204

ThPI 206

ThPI 213

L. Wheeler; John R. Perkins; Jack Henion;

Advion Biosciences, Inc., Ithaca, NY Multiple-Analyte LCIMSIMS Assay Development Strategies Applied to High Throughput Drug Discovery Pharmacokinetic Studies; Lei Zhanq; John D. Laycock; Hesham Ghobarah; Krys J. Miller; Amgen, Inc., Thousand Oaks, CA A Rapid Columnless On-Line SPE Method for LC/MS/MS Analysis in Drug Discovery; Kevarz Xu; Krys J. Miller; Amgen, Inc., Thousand Oaks, CA An Automatic 96-Well Solid Phase Extraction and Liquid Chromatography - Tandem Mass Spectrometry Method for the Analysis of Morphine, Morphine-3Glucuronide and Morphine-6-Glucuronide in Human Plasma; Wilson Shou; Man, Pelzer; Tom Addison; Xiangyu Jiang; Naidong Weng; Covance Laboratories, Inc., Madison, WI Direct Online Determination of Ipratropium Bromide by Turbulent Flow Chromatography/Mass Spectrometry;

Erick Tessier; Adlai E. Niggebrugge; Rudolf Guilbaud; MDS ThPI 205

ThPI 212

Pharma Services, Montreal, QC, Canada High-Throughput MALDI-TOF Profiling of the Mouse Brain Mitochondrial Proteome; Marv F. Looez’; Alla Bogdanova’; Annie Wang2; Simon Melov3; ‘Proteome Systems, Woburn, MA; 2Kratos Analytica, Chestnut Ridge, NY 3Buck Institute, Novato, CA Development and Validation of a High Throughput 96Well Plate Method for the Determination of NE-10235 and its Metabolite (NE-10171) in Human Whole Blood by LC-MS/MS; Larry Davey’; Terry Beard’; Laurayne

ThPI 214

Rettig’; Xiangyu Jiang’; Brian D. Beato*; Naidony Weng’;

ThPI 215

ThPI 216

ThPI 217

ThPI 207

Research Institute, Kenilworth, NJ

ThPI 218 Direct Analysis of Basic and Acidic Drugs in Rat Plasma Using On-Line Extraction LC/MS/MS; Claude Mallet; Jeff Mazzeo; Uwe Neue; Waters Corporation, Milfrd, MA ThPI 219 Hepatic Microsomal Screening of a Diverse Set of Compounds Using a 96-Well Plate Format and LClMS Analysis to Predict Oral Bioavailability; W. Perrv

Gordon’; Tom P. Hollenbeck’; Bach T. Phan’; Julie ZhangO’Brien’; Julie Doerr-Stevens*; Ron J. Christopher’; ‘Trega

ThPI 220

Eric E Niederkofler’; Urban A Kiernan’; Kemmons A Tubbs’; Karl F Gruber’; Dobrin Nedelkov’; Peter Williams’; Randall W Nelson’; ‘Intrinsic Bioprobes, Inc. Tempe, AZ; 2Arizona State Universi& Tempe, AZ Sample Throughput for the Quantitative Analysis of Amoxicillin in Human Plasma with Detection by LCMSMS: An Approach; Garnet G McRae; Ginette

‘Covance Laboratories, Inc, Madison, WI; Covance Laboratores, Indianapolis, IN A Novel In Vitro High-Throughput Approach For Assessing Metabolic Stability in Hepatic Microsomes Using Sequential Oxidation-Glucuronidation and LC/MS/MS Detection; Liann Liu; Jui-Hsiang Fan; Chris Havel; Walter Yu; Joyce Mordenti; Axys Pharmaceuticals, Inc., S San Francisco, CA A Semi-Automated Dual-Column Chiral LCOISIMS Method for the Analysis of Hydroxylated Metabolites of GW275X in Human Serum; Daniel Y. Lee; Lisa St. John Williams; John A. Dunn; GlaxoSmithKline, RTP, NC Simultaneous Evaluation of Cytochrome CYP2D6 and Cytochrome CYP3A4 Enzyme Inhibitions in Human Liver Microsomes by LCIMSIMS; Inhou Chu; Leonard

Favreau; Tony Soares; Eliza Fung; Yaoqinf Chen; Shynaz Zaman; Ronald White; Amin A. Nomeir; Schering-Plough

McMullen’; Xuan Nguven2; Yves G. Leblanc2; Charles Grandmaison*; ‘Procter & Gamble Pharmaceuticals, Norwich, NY; ‘MDS Pharma Services Inc., Saint-Laurent, QC, Canada Quantification of Human Plasma Proteins via Integration of High Throughput Mass Spectrometric Immunoassay System and Functional MALDI Targets;

Phanna Services, Montreal, QC, Canada A Fully Automated Protein Precipitation LC/MS/MS Assay Using 96-Well Technology and Track Robotic System; Scott P. Denee; Brent Butler; GlaxoSmithKline, RTP, NC High Throughput Analysis of Amino Acids in Biological Matrixes; Shaoxia Yu’; Arthur Rugg’; Lily Li’; Sharon Lu2; Frank Hsieh*; ’ Cereon Genomics, LLC, Cambridge, MA, USA; ‘Millennium Pharmaceutical Inc., Cambridge, MA, USA High Throughput Chemical Characterization of Small Molecules by DIOS-MS; Zhouxin Shen’; John E. Crowell*; M. G. Finn3; Gary Siuzdak3; ‘Mass Consortium Corporation, San Diego, CA; 2University of California, San Diego, La Jolla, CA; ‘The Scripps Research Institute, La Jolla, CA On-line Temperature-Assisted SPE-LC-MS for Fast Determination of Sirolimus in Blood; Bert Ooms; Emile Koster; Spark Holland, Emmen, Netherlands Utilization of TOF-MS Analysis with Parallel MultiSampling for High Throughput Analysis of Compounds Undergoing in vitro Metabolic Stability Testing in Human Liver Microsomes; Colleen A McNanev; James Belcastro; Kathleen W. Mosure; Kenneth Santone; Bristolk&ers Squibb Company, Inc, Walling,hord CT A Highly Automated 96-Well SPE and LCNSIMS Method for the Determination of Fentanyl in Human Plasma; Wilson Z. Shou’; David G. Busch’; Joan M.

Biosciences, Inc., San Diego, CA: ‘Neurocrine Biosciences, Inc., San Diego, CA Rapid and Sensitive Isocratic LCIMSNS Methods Used in Quantitating In Vitro Assays for a Large Diverse Set of Drugs; Thomas P E Hollenbeck, W Perry Gordon; Jesus

Castelo; Melanie Harm; Nikole Johnson; Kyoung-jin Lee; Yong Hee Lee; Deirdre Moylan; Julie O’Brien; Carleton Sage; Deirdre Moylan; Julie O’Brien; Melanie Hann; Carleton Sage; Trega Biosciences, La Jolla, CA

ThPI 208 Doubling

ThPI 221

Development of a Faster Brain/Plasma Screen vs the Standard Rat Brain Penetration Study in Drug Discovery

49” ASMS Conference on Mass Spectrotnetry

66s

ThPI 222

ThPI 223

ThPI 224

ThPI 225

ThPI 226

ThPI 227

ThPI 228

ThPI 229

ThPI 230

ThPI 23 1

ThPI 232

ThPI 233

with LC-API-MS/MS; Kwokei J. Ng; Hong Mei; Shiyong Wang; Cymbelene Nardo; Walter Korfmacher; Schering Plough Research Institute, Kenilworth,, NJ An Automated 96-Well Plate Liquid-Liquid ExtractionLC/MS/MS Procedure Using a Dual-Column Set-Up for the Determination of Double-Bond Isomers in Human Plasma; Emilv D. Fiese; Jamie J. Zhao; John D. Rogers; Nanyan Rena Zhang; Merck & Co., West Point, PA Analysis of Caco-2 Samples Using Solid-Phase Extraction and HPLC/Ion-Trap Mass Spectrometry; Hongbing Zhang; Jie Pang; Cheng Y. Yang; Li Sun; James Wei; Cho Tang; Sugen, Inc, S San Francisco, CA Multiple-Sprayer Tandem Mass Spectrometry with Parallel Turbulent Flow Extraction and Parallel Separation for High-Throughput Quantitation in Biological Fluids; Yuzhong Deng; Hang Zeng; Jing-Tao Wu; Steve E. Unger; DuPont Pharmaceuticals Company, Newark, DE Semi-Automated Screening Approach for the Estimation of Plasma Protein Binding Using LCIMSIMS; William Speed’; Silvia Portolan’; Daniele Pezzetta’; Giuseppe Cocchiara’; Mike Verburg’; Paul Bombard+; ‘Pharmacia, Nerviano, Italy; ‘Pharmacia, Kalamazoo, Ml Automated In Vilro Metabollsm Studies: Application to Screening Cathepsin K Inhibitors; Tanva Gamble’; Kevin P. Bateman’; Renata Oballa’; Jean Francois Levesque’; Osamu Okamoto’; ‘Merck Frosst Canada & Co., Kirkland, QC, Canada; 2MDS-Sciex, Concord, ON, Canada Toxicoklnetic Studies of Methylphenidate (Ritalin) in Rat, Rabbit, and Dog Using an Enantioselective LiquidSpectrometry Mass Tandem Chromatography/ Technique; Luis Ramos; Sally Aram; Ray Bakhtiar; Francis Tse; Novartis Inst. for Biomedical Res., East Hanover, NJ Development and Validation of a Rapid Screening LClMSNS Method for Simultaneous Measurement of Six Calcium Channel Antagonists in Rat Plasma by Protein Precipitation Using a Semi-Automated 96 Well Plate Format; Sadavavvan V Rahavendran; Philip Teitelbaum; Covance Laboratories, Madison, WI Metabolic Stability Screen Methos Using Stream Lined Automation in Human Liver Microsome Incubation and Generic LC/MS Analysis; Karen Wang; Kurt Hollfelder; Bonnie Fayer; Marilyn Dar; Louise Renzetti; Homann-La Roche Inc., Nutley, NJ Speeding Metabolic Stability Assays Using Automated High Throughput LC/MS Techniques; Kellv Johnson’; John Erve’; Andre Dandeneau’; Beverly Kenney’; ‘Waters Corporation, Milford, u4; 2GENTEST Corporation, Mi&x-d, MA Quantification of Nimesulide in Human Plasma by HPLC/Tandem Mass Spectrometry: Application for Bioequivalence Studies; Rafael Barrientos; Mauro Sucupira; Marcel0 Muscati, Gilbert0 De Nucci; Institute of Biomedical Sciences - USP, Sao Paul0 - SP, Brazil A Specialized High-Throughput Sample Dellvery System Utilizing a Dual-Spray LC/MS Interface for the Bioanalysis of SO0 Samples/Hour; Kevin M. Whalen; J. Carter Courtney; Ruth M. Olech; John S. Janiszewski; Katrina J. Rogers; John J. Schelhorn; Donald .J Smith; Bruce A. Jaxheimer; Mark W. Tschopp; Mark J. Cole; Pfizer Global Research and Development, Groton, CT Evaluation of Matrix Effects in High Flow ColumnSwitching On-Line Extraction LCYMSIMS for Direct Injection of Biological Fluids; Hang Zeng; Jing-Tao Wu; Alan Deng; Steve E Unger; DuPont Pharmaceutical Company, Newark, DE

ThP1234

for High-Throughput A Novel SPE System Quantification by ESI-LCIMSIMS Utilizing 96 Discrete Zones in a Disposable Card Format; Ruth M Olech’; Robert A Pranis’; Jason R Jacobson2; Craig A Perman’; Beth A Bornan*; Joshua Soldo2; Robert Speziale3; Thomas W Astle3; Mark J Cole’; John S Janiszewski’; Kevin M Whalen’; ‘Pfizer Global Research and Development, Groton, CT; 23M Filtration Products, Minneapolis, MN; ‘TomTec, Hamden, CT

ThPI 235 PHARMACEUTICALS, 236 - 251 ThPJ 236 A Strategy for Structural Elucidataion Using LClMS and LClNMI& Evaluation of a Light-Stressed Topotecan Sample; Mark A. Olsen; Paul G. Cummings; Sonya A. Kennedy-Gabb; Priscilla H. Offen; GlaxoSmithKline, King of Prussia, PA ThPJ 237 Characterization of a Pharmaceutical Intermediate of Aminotetralin Analogs by GCYMS and LCIMS; Xiaovi m; Jing Yuan; Patrick Jones; 0. David Sparkman; David S. Fries; University of the Pa@, Stockton, CA ThPJ 238 HPLC-MALDIMSIMS for Drug Impurity Profiling Using Non-Volatile HPLC Buffers; Jens Osterodt’; Dietrich A. Volmer’; Michael Schirm’; Tammy LeRiche’; KGaA, Darmstadt, Germany; Oliver Keil’; ‘Merck 2Fachhochschule Muenster, Stein@, Germany ThPJ 239 Distinguishing between Morphine and Hydromorphone: Statistical Analysis of CID Data and Deuterium Exchange Studies; John P. Caesar, Jr, John Tiani; Sigmund M. Waraszkiewicz; AstraZeneca, Westborough, MA ThPJ 240 Investigation of the Affinity Binding of Estrogen Analogues to Human Estrogen Receptors Using Ultrafdtration and LC-MS; Yongkai Sun; Chunpanp Gu; Xuemei Liu; Wenzhong Liang; Judy L. Bolton; Richard B. van Breemen; Universiq of Illinois at Chicago, I1 ThPJ 241 Use of Basic Additives with Supercritical Fluid Chromatography/Mass Spectrometry; Daniel G. Morgan; Manon Villeneuve; Lloyd W. Frick, GlaxoSmithKline, Res. Tri. Park, NC ThPJ 242 High Throughput Screening of Inhibitors of Nod Sulfotransferase Using FT-ICR MS; Xue S. Ge; Na Pi; Joshua I. Armstrong; Carolyn R. Bertozzi; Julie A. Leary; University of California, Berkeley, CA ThPJ 243 Characterization of Sorbic Acid and Its Oxidation Degradants Using HPLC-ESI-MS, and HPLC-MALDITOF MS, X. Michael Liu; E. Peter Marziarz; Matt Jonasse; John Brunette; Steve Maier; Daniel Ammon; David Heiler; Bausch & Lomb, Rochester, NY ThPJ 244 Dereplication of a Natural Product Library Utilizing OnLine and Off-Line Ion Trap Mass Spectrometry Coupled with High-Throughput Purltication; Oi Jia’; Mei-Feng Hong’; Scott Waite’; Eric Roden’; Susan Vance’; Chad Ostrande?; Peter Grosshans 2; ’ Univera Pharmaceuticals, Inc., Broomfield, CO; 2Hitachi Instruments, Inc., San Jose, CA ThPJ 245 The Application of High-Speed Chromatography for High Throughput LClMS Analysis; Byron N Kieser; Tanya Gamble; Garv lmvey; Aplpied Biosystems / MDS Sciex, Toronto, ON, Canada ThP J 246 Direct Detection of Drugs of Abuse in Human Urine Using ESI-FAIMS-MS; Roger Guevremont; David A. Barnett; Barbara Ells; Randy W. Purves; Margaret A. McCooeye; National Research Council of Canada, Ottawa, ON, Canada ThPJ 247 Structure Elucidation of some Unexpected Process Impurities in a Pharmaceutical Drug Substance by inSource CAD, Deuterium Exchange and Accurate Mass

49’ ASMS Conference on Mass Spectrometry with

an ESI-oa-TOFMS;

Pharmaceuticals

67s

John A. Castoro; DuPont

Company, Deepwater, NJ

ThPJ 248 Structure Elucidation of Chlorhexidine Related Substances by Liquid Chromatography Tandem Mass Spectrometry Using Trlshydroxymethylaminomethane and Trifluoroacetic Acid as Mobile Phase Modifiers; Evelyn E McLaughlin; Craip S Newbv; GlaxoSmithKline, Weybridge, UK

ThPJ 249 Comparison between LC/UV and LC/MS for the Characterization of Impurities Present in Trimethoprlm Tablets; Nicolas Barbarin’; Jack Henion’; Yunhui Wu*; ‘Cornell University, Ithaca, NY; 2Merck & Co., West Point, PA

ThPJ 250 Study of the Inhibition Reaction of SHV-1 BetaLactamase with Tazobactam Using Liquid Chromatography/Electrospray Ionization Mass Spectrometry; Xianz Zhou’; Reiko Simmons’; Zhaoyan Jin’; Andrea M. Huje?; Marion Helfand*; Lily M. Ng’; Robert A. Bonomo*; ‘Cleveland State University, Cleveland, OH: 2Louis Stokes Veterans Medical Center, Cleveland

OH

ThPK 260 Rapid Identification of PCR-Products by ES&Ion Tra Mass Spectrometry; Stephanie Hahner’; Uhike Schmidt 4; Dirk Wunderlich’; Carsten B%l3mann’; Arnd Ingendoh’; ‘Bruker Daltonik GmbH, Bremen, Germany; 21nstitute for Legal Medicine, Freiburg, Germany

ThPK 261 High-Resolution High Mass Accuracy Analysis of Long Oligonucleotides Using ESI-TOF Mass Spectrometer; Yelena Lvubarskava’; Brian Elliott!; Arthur Sims’; ‘Applied Biosystems, Framingham, MA; Technologies, Inc., Coralville, IA

2Zntegrated

DNA

ThPK 262 Effects of Ring Modifications on Oligonucleotlde Sequencing by Mass Spectrometry; Jef Rozenski; Arthur Van Aerschot; Piet Herdewijn; Rega Institute for Medical Research, Leuven, Belgium

of ThPK 263 Validation a High-Throughput Electrospray Ionization Mass Spectrometry Method for the Detection of Single Nucleotide Polymorphisms; Sheng Zhang; Colleen K. Van Pelt; Xian Huang; Gary A. Schultz; Advion Biosciences, Inc., Ithaca, NY

ThPJ 25 1 Residual Solvents Determination in Pharmaceutical Products by SPME-GC-MS; Costin C. Camarasu; Gedeon

ThPK 264 Direct Measurement and Positional Mapping of Damaged Residues in Ollgonucleotides; Hai Luo; Christophe D. Masselon; Mary S. Lipton; Richard D. Smith;

NUCLEIC ACIDS, 252 - 277 Develop and Validate MALDI MS and ES1 LC/MS/MS Methods for Determination of Estrogen Quinone-induced Apurinic Sites and Adducts; Li-Kang Zhang’; Kimberly A. Chapman*; Jiang Zhao’; Michael L. Gross’; Ercole L. Cavalieri*; Eleanor G. Rogan*; ‘Dept. of Chemistry,

ThPK 265 ESI-FTICR Mass Spectrometric Analysis of QC-PCR Products to Simultaneously Detect and Quantify Two Protozoan Parasites (Haplosporldium Nelsoni And Perkinsus Marinus) in the Eastern Oyster (Crassostrea Virginica); Sevau Koleiian; David C. Muddiman; James C. Harris; William T. Brooks; Bonnie L. Brown; Virginia

__ _.. _.1. Ktcnter Ltd., Budapest, Hungary

ThPK252

Pactjic Northwest National Laboratory, Richland, WA

Washington University, St. Louis, MO: ‘Univ. of Nebraska Medical Center, Omaha, NE

ThPK 253 Rapid Validation of DNA Sequence Using Mass Spectrometr Coupled With Nucleotide Mass Tagging; Fadi A. Abdi ; Tom Shattuck*; Mark Mundt’; Vahid Majidi’; Xian Chen’; ‘Los Alamos National Laboratory, Los Alamos, NM; 2Colby College, Waterville, ME

ThPK 254 RNA Neomycin Complexes Analyzed by Electrospray and UV-MALDI-MS; Julia Gross’; K. Asish Xavie?; Michael L. Gross’; ‘Washington University Dept. of Chemistry, St. Louis, MO; 2Message Pharmaceuticals, Malvern, PA

Inc.,

ThPK 255 MALDI and ES1 Analysis of DNA with Rhodium, Clsplatin, and Carboplatln Adducts; John M. Koomen; Mijeong Kang; Kim R. Dunbar; David H. Russell; Texas .^.___ A&M University, College Station, TX

Morgantown, WV; 2Marshall University, Huntington, 3Yale University New Haven, CT

WV;

ThPK 257 Accurate Genotyping of Simple and Compound Short Tandem Repeat Loci Using Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry; Allison P. Null; James C. Hannis; David C. Muddiman; Virginia Commonwealth University Richmond VA

ThPK 258 Kinetics of Deamination of Cytosine and 5Methylcytosine Photoproducts Measured on an ES1 IonTrap Mass Spectrometer; Yinshenz Wang; John-Stephen Taylor; Michael L. Gross; Washington University, St. Louis, MO

ThPK 259 Ollgonucleotlde Binding Affkities of Naturally Occuring Polyamlnes by Ion Trap Mass Spectrometry; Girish S. m’; William D. Price*; Patrick S. Gallery’; I West Virginia Morgantown, WV

WV;

‘Marshall

University, Richmond

VA

Virginia Commonwealth

University, Richmond,

University,

VA

ThPK 267 Evaluation of Complexatlon of Metal Mediated DNA Binding Drugs to Oligonucleotides vla Electrospra Ionization Mass Spectrometry; Michelle L. Revzer P; Wendi David*; Jennifer S. Brodbelt*; ‘Vanderbilt University, Nashville, TN; 2The Universify of Texas, Austin, TX

ThPK 268 A Simple Purification Procedure of PCR Products for Electrospray Ionization Mass Spectrometry; Yuu Jiang; Kristin A. Sannes-Lowery; Len L. Cummins; Sherilynn M. Manalili; Jared J. Drader; Steven A. Hofstadler; Ibis Therapeutics - A Div. of Isis Pharm, Carlsbad

ThPK 256 Noncovalent Interactions of an Azetidine Analouue of a Polyamine with Oligonucleotides by Ion Trap Mass Spectrometry; Girish S Gudi’; William D Price*; Faye A Rogers3; Patrick S Callerv’; ‘West Virginia University,

University, Huntington,

Commonwealth

ThPK 266 Extensive Evaluation of Novel Sample Preparation Techniques for High-Sensitivity Mass Measurements of Nucleic Acids Using ESI-FT-ICR Mass Spectrometry; Laura T. GeorPe; Allison P. Null; David C. Muddiman;

CA

ThPK 269 A Method for Determining Binding Constants of NonCovalent Complexes by Electrospray Ionization Mass Spectrometry with No Approximation on the Response Factors; Valerie Gabelica ; Frederic Rosu’; Nives Galic’; Frederic Rosu’; Claude Houssie+; Edwin De Pauw’; ‘Biospectroscopy Laboratory - ULg, Liege, Belgium; ‘Mass Spectrometry Laborato y - ULg, Liege, Belgium

ThPK 270 Approaches for High-Throughput Analysis of Posttranscriptionally Modified RNA; Beniam T. Berhane; Patrick A. Limbach; Lousiana State University* Baton Rouge, LA

ThPK 271 MS/MS Study of Deoxydinucleotides Bound with AlhaliMetal Ions Using ESI-MS; Zeoer Abliz’; Mitsuo Takayama’; Yun Xiang’; Li-jun Li’; ‘Chinese Academy of Medical Sciences, Beijing, China; 2Toho University Japan

Chiba,

ThPK 272 Preparation of Single-Stranded PCR Amplkons by Physical and Enzymatic Methods for Determination by ESI-FTICR-MS; John B. Mangrum; Allison P. Null; Jason W. Flora; David C. Muddiman; Virginia Commonwealth University, Richmond,

VA

68s

49” ASMS Conference on Mass Spectromehy

ThPK 273 Introduction of Thymine Glycol Stereoisomers into Oligomeric DNA; Robert A. Rieaer; Charles R. Iden; State University of New York, Stony Brook, NY

ThPK 274 Identification of Compounds Non-Covalently RNA Using GPC Spin Columns with ES1 MS; Keiko Tabei; Anthony Amin; Matthew Olson; Bebemitz; Marshall M. Siegel; Wyeth-Ayerst

Bound to Hui Tong; Geraldine Research,

Pearl River, NY

ThPK 275 Characterization of Two Novel Uridine Derivatives in Mammalian Mitochondrial tRNAs; toward Elucidation of the Molecular Mechanism for Human Mitochondrial Diseases; Takeo Suzuki; Tsutom Suzuki; Takeshi Wada; Kazuhiko Saigo; Kimitsuna Watanabe; University of Tokyo, Tokyo, Japan

of Polymer ThPK 276 Rapid Methods for the Removal Contaminants, Detergents, and Salts from Oligonucleotides for ESI-MS Analysis; Sherilvnn M. Manalili; Jared J. Drader; Steven A. Hofstadler; Ibis Therapeutics - A Div. of Isis Pharm, Carlsbad, CA

ThPK 277 The Effect of DMSO Complexes Analyzed Spectrometry; Jessica Cathy D. Moore; Pfizer

on Non-Covalent Oligonucleotide by Electrospray Ionization Mass M. Robinson; Michael J. Greig; Global R@

- La Jolla, San Diego,

CA

University, Louis, MO

Piscataway,

NJ; 2Washington

University,

St.

ThPL 288 Interaction of Metal Ions with Oxidized Insulin Chain A Probed by Electrospray Ionization Mass Spectrometry; Yen-Peng, Ho; Hsing-Piao Li; National Dong Hwa University Chem. Dept., Hualien, Taiwan, ROC

ThPL 289 Kinetic Distinction of the Leucine-Isoleucine Isomers by ESI-MS with Exopeptidases; Jennifer L. Rutherford; William D. Price; Marshall University, Huntington, WV ThPL 290 Irreversible Caspase Inhibitors as Molecular Mass Tags for Apoptosis Investigation; John T. Mehl; Ji-Eun Kim; George N. Nikov; Michael H. Cardone; John S. Wishnok; Steven R. Tannenbaum; Massachusetts Institute of Technology, Cambridge, MA

ThPL 291 Pre-Steady State Kinetics of Chymotrypsin Monitored by Stopped-Flow ES1 MS; Jaaan M. Pavidioartv; Andrew S. Deonarine; Beata M. Kolakowski; Lam Konermann; The University of Western Ontario, London, ON, Canada

ThPL 292 Miniaturized Enzyme Reactors Protein for Identification; Wendv D. Dominick; Patrick A. Limbach; Louisiana State University, Baton Rouge, LA

PEPTIDES: SPECIAL TOPICS, 278 - 314 ThPL 278 Identification of Peutides Involved in T Cell Development; Kenneth-L. Johnson’; W. Chad Kiepe?; Stephen C. Jameson*; Stephen Naylor’; ‘Mayo Clinic, Rochester, MN; 2University of Minnesota Minneapolis, MN

Medical School,

ThPL 279 Optimized Conditions for Isotopic Labeling HLAPresented Peptides; Julie A. Corbo; Alexandru C. Lazar; Andy J. Tomlinson; ZYCOS Inc., Lexington, MA ThPL 280 Improved Protocol for Stable Isotope Labeling and Orthogonal 2-Dimensional Nunoflow LC-ESI/MS in MHC Class I Associated Peptide Identification; Hugo D. Meirinq; Carla A. Herberts; Ed van der Heeti, Jan ten Hove; Cecile A.C.M. van Els; Ad P.J.M. de Jong; Natl. Inst. Public Health & Environment,

Bilthoven, The Netherlands

ThPL 281 Identification of Modified Antigenic HEL Peptides Bound to MHC Class II Molecules; Koen van der Drift’; Ilan Vidavsky’; Carlos Velazquez’; Emil R. Unanue*; Michael L. Gross’; ’ Washington University, St. Louis, MO; 2School of Medicine, Washington Univ., St. Louis, MO

ThPL 282 Peptide Selection by the Diabetogenic Class II MHC Molecule, I-Ag7 - Progress toward Finding the Juvenile Diabetes Antigen; Ilan Vidavskv; Koen Van Der Drift; Michael L. Gross; Anish Suri; Osami Kanagawa; Emil R. Unanue; Washington University, St. Louis, MO ThPL 283 Mass Spectrometry and Metal-Catalyzed Oxidation Reactions: Determining the Coordination Structure of Metalloproteins; Jihveon Lim; Richard W. Vachet; University of Massachusetts, Amherst, MA

ThPL 284 MALDI-TOFMS Study of the Conjugates of Hen Egg White Lysozyme with “Fischer” Metallocarbene Complexes; Michele Salmain’; Gerard Jaouen’; Jean-Claude Blais ; ‘CNRS - Ecole Nationale Superieure Chimie, Paris, France; ‘CNRS - Universite Pierre et Marie Curie, Paris, France

ThPL 285 Direct Screening Methods for Fast Identification of Heavy Metal Derivatives in Protein Crystals and Subsequent Peptide Mapping; Klaus Hoevel; Lejon Martens; Guido Sonsmann; Dietmar Schomburg; Institute of Biochemistry,

ThPL 287 ES1 MS Titration of Peptides by Sodium Ions Gives the Number of Carboxylate Groups; Olea Timofeev’; Mei Zhu*; Michael L. Gross’; ‘College of Pharmacy, Rutgers

Cologne, Germany

Ionization Mass ThPL 286 Electrospray Spectrometry of Protonated and Metalated Peptides; Michelle M Kish; Chrys Wesdemiotis; The University ofdkron, Akron, OH

ThPL 293 Assay of Proteolytic Activity in a Protein Purification Process Using MALDI-TOF MS; Zhirui Lian; John V Amari; David W Andrews; BioChem Pharma Inc., Northborough,

MA

ThPL 294 Investigation of Enzyme Kinetics Using ES&MS; Xue S. Gel; Martin K. Beyerz; Tammv L. Sirich’; Julie A. Leary’; ‘University of California, Berkeley, CA; Universitaet Muenchen, Garching, Germany

2Technische

ThPL 295 A Comparison of Epitope Mapping Methods as Applied to V Antigen from Yersiniu pestis; Kerrv Anderson’; Jim Hill*; Sophie Smithe?; Richard W Titball’; Dominique Despeyroux’; ’CBD Porton Down, Detection Department, Salisbury, UK; 2CBD Porton Department, Salisbury, UK

Down,

Microbiology

ThPL 296 Optimisation of the Automated Proteolysis of Proteins via Pepsin and Trypsin Immobilised Enzyme Cartridges Prior to Electrospray Mass Spectrometry tESI-MS): Sarah K West; Meg E Green; Dominique Despeyroux; Kerry Anderson: CBD Porton Down, Salisburv, UK ThPL 297 Screening Protein Fractions for Enzymatic Activities with Mass Spectrometry; Hartmut Schluter; Joachim Jankowski; Free University of Berlin, Berlin, Germany ThPL 298 Kinetic Analysis of PKA Catalyzed Reactions Using ESIMS; Darrvl Davis; Catherine Bentzley; University of Sciences in Philadelphia, Philadelphia,

PA

ThPL 299 Identification of the N-terminal Lipid Modification of G Protein as by Mass Spectrometry; Eberhard Krause’; Christiane Kleuss*; ‘FI firer Molekulare Pharmakologie, FMP, Berlin, Germany; ‘Institut fuer Pharmakologie, Berlin, Germany

FU

ThPL 300 Detection of Arginine Dimethylated Peptides by Precursor Ion Scanning Quadrupole TOF Mass Spectrometry in Positive Ion Mode; Juri Raoosilber’; Westley J. Friesen’; Gideon Dreyfuss*; Matthias Mann’; ‘University of Southern Denmark, Odense, 2University of Pennsylvania, Philadelphia, PA

Denmark;

ThPL 301 Rapid Characterization of Disultkle Bonds Using Automated On-Column Micro-Chemistry for Sample Preparation and Mass Mapping; Brett S. Phinney; JoseLuis Gallegos-Perez; J. Throck Watson; Michigan State Universi@ East Lansing, MI

ThPL 302 Fragmentation of Disulfide-Linked Peptides and their Flxed-Charge Derivatives by ESI-CAD-MS/MS; Wei Wu;

49” ASMS Conference

ThPL 303

ThPL 304

ThPL 305

ThPL 306

69s

on Mass Spectrometry

Jason Manley; J. Throck Watson; Michigan State University East Lansing, MI Characterizing Closely Spaced, Complex Disultlde Bond Patterns in Peptides and Proteins by Liquid ChromatographylElectrospray Ionization-Tandem Mass Spectrometry; Ten-Yana Yen; Hui Yan; Bruce A Macher; San Francisco State University, San Francisco, CA of Posttranslational Analysis Modifications by Trap Atmospheric Pressure MALDI/Ion Mass Spectrometry; Lisa A. Marzilli; Susanne C. Moyer; Robert J. Cotter; Johns Hopkins University, Baltimore, MD Protein Analysis Using N-terminal Labeling and Mass Spectrometry; Toshivuki Mikami; Kazunori Yanagi; Hiroshi Nakazawa; Sumitomo Chemical Co., Ltd., Takarazuka, Japan Fragmentation Analysis of Peptide Nucleic Acids (PNAs) by MALDI and ES1 Mass Spectrometry; Auette Jacob; Achim

Stephan;

Joerg

Hoheisel;

Martina

ThPM316 Rapid Quantitative

Determination of Lysophosphatidic Acids (LPA) by Liquid Chromatography Taqdem Mass Spectrometry (LC-MS/MS); Jean M. Lacey ; Mark J.

Magera’; Bruno N. Casetta’; Piero Rinaldo’; Dietrich Matem’; ‘Mayo Clinic, Rochester, MN; ‘Applied Biosystems, Monza, Italy

ThPM 317 Identification Carbapenem Formulations;

Laboratories,

West Point, PA of a Simple MS/MS Screening Assay for Phenylketonuria: Clinical Trial Evaluation and Cutoff Determination; Blas A. Cerda’; Michael E. Jackson’;

Wendy Barnhart’; Susan Leonard’; Chet Knopsnider’; Jamie Klusty’ ; Evelyn Than’; Lori Vance’; Marlinda Iye?; William B. Becke?; ‘PerkinElmer Life Sciences, Akron, OH; 20hio Department of Health, Columbus, OH Quantification of Soy Isoflavones and Testosterone in Plasma from a Prostate Cancer Clinical Trial; Daniel R. Doerge’; Nathan C. Twaddle’; Mona I.

ThPM 319 LCAMS

Churchwell’; Michael McCullagh’; Steve Baiic2; Fazul H. Sarka?; Maha Hussain3; Omer Kucuk3; ‘National Ctr fir

ThPM 320

ThPL 308 Optimizing Data Collection for Bacterial Spores Analysis Using MALDI-MS; James R. Stutler’; Joany Jackman’; Karen L. Wahl’; Kristin H. Jarman’; Sharon T. Cebula2; ‘The Johns Hopkins University, Laurel, MD; 2Pactjic Northwest National Laboratory, Richland, WA ThPL 309 Direct Gas-Phase Peptide Fingerprinting of E. coli 70s Ribosomal Proteins Using Ion Mobility - Time-of-Flight and Fourier Transform Ion Cyclotron Resonance Mass Spectrometry; Gina A. Zientara; Claire J. Ross; David E. Clemmer; Indiana University, Bloomington, IN ThPL 310 Characterization of the P13 Membrane Protein of Borrelia Burgdorferi by MALDITOF- ESI-qTOF- and ESI-FTICR-MS; Carol L. Nilsson’; Helen .J Coope?;

ThPM 321

ThPM 322

Kristina Hakanssor?; Alan G. Marshal12; Yngve Ostberg3; Sven Bergstrom3; ‘CIMAR, NHMFL, Tallahassee, FL; 2Umea University, Umea, Sweden; 3Goteborg Universiv, Goteborg, Sweden ThPL311 Structural Characterization of Peptidoglycan Muropeptides from Helicobacter pylon’ by Nanoeiectrospray Tandem Mass Spectrometry; Geiina Denq; Boudewijn L. M. de Jonge; April Blodgett; AstraZeneca R&D Boston, Waltham, MA ThPL 312 Mass Spectrometric Identifications of the Proteins Present in Caulobacter Crescentus Stalks; Jonathan A.

ThPM 323

ThPM 324

ThPM 325

w; Marcia M. E. Ireland; Richard L. Beardsley; Ellen M. Quardokus; Yves V. Brun; James P. Reilly; Indiana University, Bloomington, IN of In Vitro Conversion of Selenocysteine to Dehydroalanine in Selenoprotein P and Determination of DisuliIde Linkage in its Short Form;

ThPL 313 Characterization

Shuauang Ma; Kristina E. Hill; Raymond F. Burk; Richard M. Caprioli; Vanderbilt University, Nashville, TN ThPL 314 Copper Ion Induces Conformational

Changes of Titin PEVK Peptldes Monitored by ES&MS, CD and NMR, Kan Ma; Kuan Wang; LPB/NIAMSAYH, Bethesda, MD

CLINICAL CHEMISTRY, 315 - 340 and Validation of Serum 17 a-Hydroxy Progesterone LClMSfMS Assay for Screening and Monitoring of Congenital Adrenal Hyperplasia; Dwaine Machacek; Ravinder J. Sin& Mwo Clinic, Rochester, MN

ThPM 315 Development

of a Novel Assessment

ThPM 318 Development

Szekelv-Klenser’; Anshu Gambhi P ; Lewis P. Woodson’; ‘3M Corporate Analytical Technology Ctr., St. Paul, MN; 23M Medical Surgical Division, St. Paul, MN

of Degradates in Safety

Zhonaxi (Zackl Zhao; Om Ahnarsson; XueZhi Qin; Robert A. Reed; Dominic P. Ip; Merck Research

Schnoelzer;

German Cancer Research Center, Heidelberg, Germany ThPL 307 A Comparative Study of Proteins Expressed by Bacillus Stearothermophilus Spores, ATCC 7953 from Two Different Starter Cultures Using MALDI-TOF and Gabriella Nano-ES1 LC/MS/MS Mass S ectrometry;

by LC/MS Antibiotic

ThPM 326

Toxicological Research, Jefferson, AR; 2Finnigan MassLab, Manchester, UK; 3Karmanos Cancer Institute, Detroit, MI Analysis of Urinary Nicotine Metabolite Profdes by LC Atmospheric Pressure Ionization Tandem Mass Spectrometry; Yang Xia; James E. McGuffey; Lanqing Wang; Connie S. Sosnoff, John T. Bemert; CDC/NCEH, Atlanta, GA Carcinogenic Estrogen Metabolites and Breast Cancer: Liquid Chromatography / Electron Capture Atmospheric Pressure Chemical Ionization / Tandem Mass Spectrometry - Based Determination of Phenotypes of Estrogen Metabolism; Aleiandro Gutierrez; Santosh Tilve; Peter O’Dwyer; Ray Boston; Ian A. Blair; University of Pennsylvania, Philadelphia, PA On the Development and Implementation of MS Reference Methods for Quantitation of ClinicallyRelevant Compounds; Maria Ospina; Adrian R Woolfitt; John R Barr; Anne E Boyer; Centers for Disease Control, Atlanta, GA Electron Capture GC/MS Determination of GammaAminobutyric Acid in Plasma; Ann Abraham; Dirk Dhossche; Loma Bland; F. Aladar Bencsath; University of South Alabama, Mobile, AL Lipid Aldehyde Characterisation by I&PC-MS-MS; h C O’Brien-Coker; Anthony I. Mallet; University of Greenwich, London, UK Electrospray Tandem Mass Spectrometry of Beta Chain Haemoglobin Variants; Dilin K. Rai; Britta Landin; Gunvor Alvelius; William J Grifflths; Karolinska Znstitutet, Stockholm, Sweden Quantitation of Cyclosporin A in Whole Blood by Stable Isotope Dilution-Electrospray-LC/MS; Fulvio Maam’;

Sonia Pereira2; Massimo Leoni3; Paride Grisenti3; Marzia Galli Kienle’; ‘ZRCCS San Rajixele, Milan, Italy; 2University of Milano-Bicocca, Milan, Italy; ‘Poli Industria Chimica SPA, Milan, Italy ThPM 327 Simultaneous Determination of Phytoestrogens, Mycoestrogens, and Synthetic Estrogens with GC-MS in Biological Specimens; Bona Chul Chung’; Man Ho Choi’; Kyoung-Rae Kim2; Jong Ki Hong3; ‘Korea Institute of Science & Technology Seoul, Korea; ‘Korea Basic Science Institute, Taejeon, Korea ThPM 328 Analysis of a Glucose Tetrasaccharide Biomarker in Urine and Plasma by Electrospray Ionization Tandem

49’h ASMS Conference

70s Sarah P. Young; Robert D. Stevens; Chen; David S. Millington; Duke University Medical Center, Durham, NC A Stable Isotope Dilution Method for the Determination of Endogenous Levels of 5-methyltetrahydrofolic Acid in Human Plasma by LC-ES1 Ion Trap MS; Vincent P. Flanagan; Robert J. Pawlosky; Food Composition Lab, BHNRC, ARS, USDA, Beltsville, MD Adaptation and Modification of Neonatal Screening by Tandem Mass Spectrometry Techniques Using the Finnigan TSQ Instrument; Harry C. Hawkins; Jacqueline S. Scott; Richard H. Scheel; Michigan Department of Community Health, Lansing, MI Routine Application of High Speed GCITOF-MS and AMDIS for Metabolic Profiling; John M Halket’; Anna M Przyborowska’; Lu Lin*; Nick P Bukowski*; ‘Royal Holloway, Universiiy of London, Egham, UK; 2Thermo Finnigan, Manchester, UK The Development and Validation of a Rapid LC-MS/MS Method for the Monitoring of Cyclosporin A in Whole Blood at Peak and Trough Concentrations; Donald P.

by LCMS; Bin Fan; James Stewart; University of Georgia, Athens, GA AGRICULTURAL MS, 341- 360

Mass Spectrometry;

Yan An; Yuan-Tsong

ThPM 329

ThPM 330

ThPM 331

ThPM 332

Cooper’; Brian G. Keevil’; Michael R. Morris’; Norman B. Roberts3; David P. Tierneg; ‘Micromass UK Limited, Dept., Wythenshawe Manchester, UK; 2Biochemistry Hospital, Manchester, UK; ‘Royal Liverpool University Hospital, Liverpool, UK ThPM 333 Comparison of Electrospray Ionization and MatrixAssisted Laser Desorption Ionization Mass Spectrometry for the Characterization of Small Drug Molecules; Tammv LeRiche’; Dietrich A. Volme?; ‘Merck Frosst, Kirkland, QC, Canada; 2Merck, Darmstadt, Germany ThPM 334 Direct Nanospray Mass Spectrometry of Lipids at the Thin-Layer Excised Level in Sub-Microgram Chromatographic Spots; David C. Young’; D. Branch Mood?; Catherine E. Costello’; ‘Harvard Medical School, Cambridge, MA; 2Boston University School of Medicine, Boston, MA ThPM 335 LCIMSIMS Detection of Endogenous Anti-Retroviral Nucleoside Triphosphates on a New Orthogonal Geometry ES1 Source; Sanjay, P Kawle’; Cynthia, V

Fletcher’; Leo Bonilla’; Julie Home?; Rohan Thaku?; Minneapolis, MN; Minnesota, I University of 2ThermoFinnigan, San Jose, CA Desorption Mass Spectrometric Screening for Biomarkers in Diagnosis of Inherited Metabolic Disorders; Michael W. Belford; Richard A. Yost; University of Florida, Gainesville, FL ThPM 337 Development of a Rapid Method for the Measurement of Total Homocysteine in Plasma Using a 96-Well Plate; Scott D. Gillingwater’; Steven McCann*; Charles Tume?; Michael M. Morris’; Donald P. Cooper’; ‘Micromass UK Dept., ‘Biochemistry UK; Manchester, Limited, Wythenshawe Hospital, Manchester, UK; ‘Paediatric Dept., Guy’s Hospital, London, UK ThPM 338 A Confirmatory Method to Detect Exogenous by Gas Epitestosterone Administration in Urine Chromatography / Combustion I Isotope Ratio Mass Spectrometry; Rodrigo Aauilera; Don H. Catlin; University of California, Los Angeles, CA ThPM 339 Detection of Bacteria in Urine Samples by Py-MAB-MS;

ThPM 336 Laser

Simon Letarte’; Daniel Morencd;

Michel J. Bertrand’;

‘Univ. of Montreal, Dept. Chemistry Montreal, QC, Canada; ‘Univ. of Montreal, School of Medecine, Montreal, QC, Canada ThPM 340 Determination of Didanosine(ddI)/ Stavudine(d4T)i Ritonivir HIV Triple-Drug Cocktail in Human Plasma

on Mass Spectrometry

ThPN 341 Sampling Strategies for SPME GClMS Investigations of Host Emanations in the Role of Mosquito Attraction; M_

Todd Huml’; Uhich R. Bernie?; Donald R. Barnard*; Richard A. Yost’; ‘University of Florida Dept. of Chemistry,

ThPN 342

ThPN 343

ThPN 344

ThPN 345

Gainesville, FL; 2U.S. Department of Agricultural Research, Gainesville, FL LC-APCIIMSIMS Analysis of Ractopamine, a GrowthPromoting Agent, in Livestock Tissues; Daniel R. Doerge’; C. Lee Holder’; Mona I. Churchwell’; Steve Preece3; David Little3; David J. Smith’; ‘Nat Ctr for Toxicological Research, Jefferson, AR; 2Micromass, Manchester, UK; ‘UDSA-ARS, Fargo, ND Strategy for Confirming Multiple Classes of Animal Drug Residues in Eggs: Generic Solid Phase Extractions and Gradient Liquid Chromatography with DataDependent Electrospray-MS/MS on an Ion Trap Mass Spectrometer; David N. Heller; Wei Cui; FDA Center for Veterinary Medicine, Laurel, MD LCIMSIMS Analysis of Carbadox and Desoxycarbadox Residue; Benjamin P.-Y. Lau; David Lewis; Health Canada, Ottawa, ON, Canada Determination of Antibiotics in Biological Matrices Liquid and Membrane Liquid Supported Spectrometry; Chromatography-Electrospray-Mass

Mathew Muzi Nindi; Patrick Ramotlopi; Titus M. Msagati; University of Botswana, Gaborone, Botswana of Potential Allelopathic Compounds Extracted from Olyza sativa; Walter J. Gwathney’; John D. Mattice’; Chuihua Kong3; ‘Statewide Mass Spectrometry Facility, Fayetteville, AR; ‘University of Arkansas, Fayetteville, AR; ‘South China Agricultural University, Guangzhou, P. R. China ThPN 347 Purge and Trap -Thermal Desorption GClMS Analysis of Flavor and Aroma in Cocoa Varieties; Eric D. Butrvm’; Kasmani Pardan*; ‘Scientific Instrument Services, Inc., Ringoes, NJ; 2Fikrah Technology, Bsdn., Kuala Lumpur, Malaysia ThPN 348 Rapid Determination of Catechin, Quercetin and Resveratrol in Wine by FIA-SRM-APCI Tandem Mass Spectrometry; Andrea Raffaelli’; Angela Cuzzola*; Piero Salvadori’; ‘CNR - Centro Studi Macromolecole, Piss, Italy: 2Dipartimento di Chimica e Chim. Ind., Pisa, Italy ThPN 349 Comparison of Electronic Nose, SPME-GCIMS and LCMS/MS Techniques to Study the Oxidation of Milk Powders during an Accelerated Storage Test; Francois

ThPN 346 Characterization

Fenaille’; Pascal Mottier’; Piero Visani’; Christiaa Rene Fumeauxi; Robert J. Turesky; Richard Stadler’; Philippe A. Guy’; ‘Nestle Research Center, Nestec Ltd., Lausanne, Switzerland; 2Division of Chemistry NCTR, Jefferson, AR ThPN 350 Human Sensory Assessment and Instrumental Analysis of Petrochemical Taint in Seafood Exposed to Crude Oil and Diesel Oil Contaminated Water; F. Aladar Bencsath’;

Terriann I. Reillg; Michael DiLiberti*; Hufnagle3; James D. Bamett4; ‘National

Lawrence

C.

Oceanic and Atmospheric Adm., Gloucester, MA; 2U.S. Food and Drug Administration, Dauphin Island AL; ‘National Oceanic and Atmospheric Adm., Seattle, WA; 4Food and Drug Administration, Bothell, WA ThPN351 Improved Detection of Flavonoids in Kale and Grapefruit Using Post-Column Metal Complexation Electrospray Ionization Mass Spectrometry; Mary B.

Sattertield’;

Junmei

Zhanq’;

Jennifer

S.

Brodbelt’;

‘University of Texas, Austin, TX; ‘NIST, Gaithersburg, MD

49’ ASMS Conference

on Mass Spectrometry

71s

ThPN 352 Localization

of Glutamine Residues in a HMW Gluten Domain Modified In-Vitro by Transglutaminase; Sander

Piersma’; Boumat&

Gerrit

Wijngaards’;

Harry

Gruppen3;

Hans

‘Centre for Protein Technology TNO-WU Wageningen, The Netherlands; ‘TN0 Nutrition and Food Research, Zeist, The Netherlands; 3Wageningen University, Wageningen, The Netherlands ThPN 353 MALDI MS Peptlde Mapping of High MW Glutenin Subunits lBx7, Ax2* and lDyl0 in Cheyenne Cultivar;

Sabrina Di Giorai2; Salvatore Rosaria Cozzolino’; Fisichella2; Domenico Garozzo’; Domenico Lafiandra3; ‘CNR-ICTPM Angela Palermo2; Catania, Italy;

ThPN 354

ThPN 355

ThPN 356

ThPN 357

2Department of Chemical Sciences, Catania, Italy; ‘Department of Agrobiology and Agrochemis, Viterbo, Italy Fast GC/MS Determination of Volatile Constituents in Matrices Containing Heavy Components; Christian Soulier’; Albino Sironi’; Faust0 Munat?; ‘Therm0 Finnigan, Paris, France; 2Thermo Finnigan, Milan, Italy High-Throughput Analysis of Agrochemicals by Photoionization MS; Matthew D. Evans’; Brian J. Nies’; Daniel B. Kassel’; ‘Syagen Technology, Inc., Tustin, CA; 2Dupont Pharmaceutical Research Lab, San Diego, CA A Highly Sensitive and Specific LC/MS/MS Method for the Simultaneous Quantitation of S stemic Chemicals in Ornamental Plants; Minghong Jia v; Wan Yong Feng’; Claudio C. Pasian’; James D. Metzger’; ‘Horti. and Crop Sci, The Ohio State Univ, Columbus, OH; 2Pharmacy College, The Ohio State &iv, Columbus, OH Differentiating 3788 Da Isoforms of Pea Albumin Using Peptidase Digestion, MALDI-TOF MS and PSD Sequencing; Wesley G. Taylor’; Douglas J. H. Olson2;

Daniel H. Sutherland’; Andrew R.S. Ross’; Paul G. Fields’; ‘Agriculture and Agri-Food Canada, Saskatoon, SK, Canada; 2National Research Council Canada, Saskatoon, SK, Canada ThPN 358 Characterisation of the Thylakoid Proteome of Pea and Arabidopsis; Jean-Benoit Pehier’; Olof Emanuelsson2;

Andrea Rudella’; Giulia Friso’; Jimmy Ytterberg’; Dario Kaluma3; Peter Roepstorfp; Gunnar van Heijne2; Klaas J van Wijk’; ‘Stockholm University, Stockholm, Sweden; ‘Odense University, Odense, Denmark; 3Cornell University, Ithaca, NY Reversed Phase Liquid Chromatography ThPN 359 Ion-Pair Electrospray Tandem Mass Spectrometry for the Simultaneous Analysis of 68 Underivatized Amino Acids;

Konstantinos

Pet&is;

Claire

Elfakir;

Michel

Dreux;

University of Orleans, Orleans, France ThPN 360 Analysis of Folate Metabolites in Green Plant Tissue; Zhenzhan Chang; Douglas A. Gaze; Michigan State University, East Lansing, MI MS IN ART, 361- 362 of the Mastic Resin Polymeric Fraction in Art Objects by Electrospray LC/MS; Pieranaela Palma;

ThPO 361 Characterization Achille

Cappiello;

Michela

Maione;

Filippo

Mangani;

Universita di Urbino, Urbino, Italy ThPO 362 Influence of Sunlight on Oxidation and Yellowing of Natural Resins Used as Varnishes on Artwork; Patrick

Dietemann; Christian Sudano; Moritz Kalin; Richard Knochenmuss; Renato Zenobi; Swiss Federal Institute of Technology, Zurich, Switzerland Level Analysis of Watercolor Paints Using Laser Desorption/Ionization Mass Spectrometry; Donna M. Grim; John Allison; Jay Siegel; Michigan State University, East Lansing, Ml

ThPO 363 Molecular