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TOPOGRAPHIC DISTRIBUTION OF ORAL MUCOSITIS IN PATIENTS WTIH ADVANCED ORAL CANCER TREATED WITH PROPHYLACTIC PHOTOBIOMODULATION
OOOO Volume 129, Number 1 DOMINGUES VARGAS
MARTINS
ABSTRACTS
e149
and,
PABLO
AGUSTIN
Objective: To determine the proteome profile of adenoid cystic carcinoma (AdCC) and polymorphous adenocarcinoma (PAc) and to identify a protein signature useful to differentiate both neoplasms. Study Design: Ten cases of AdCC and 10 cases of PAc were retrospectively retrieved and submitted to laser microdissection for enrichment of neoplastic tissue. The samples were submitted to liquid chromatography-tandem mass spectrometry (LC-MS/MS) and the proteomics data were analyzed using the MaxQuant software. MS/MS spectra were searched against the Human UniProt database, whereas statistical analyses were performed with Perseus software. Results: The LC-MS/MS analysis identified 1957 proteins. Among these, 261 proteins were exclusively identified in AdCC and 106 proteins only in PAc. Clustering analysis of the 394 statistically significant proteins identified in both tumors showed a clear separation of the tumors. A total of 16 proteins were upregulated in each tumor, 6 in AdCC and 10 in PAc, and their cluster analysis also revealed clear differences between both neoplasms. Conclusions: Global proteome can effectively discriminate AdCC and PAc, and the use of a protein signature may represent a useful diagnostic auxiliary to differentiate both tumors in difficult cases. Support: FAPESP 2015/16056-0 and FAPEMIG.
EFFECT OF MANDIBLE ALTERATIONS ON THE PROGNOSIS OF PATIENTS WITH ORAL SQUAMOUS CELL CARCINOMA. ERICA FERNANDA PATRICIO DA SILVA, GUSTAVO DAVI RABELO, CLOVIS ANTONIO LOPES PINTO, VINICIUS FERNANDO CALSAVARA, LUIZ PAULO KOWALSKI and, FABIO ABREU ALVES Objective: This study aimed to evaluate the effect of mandible changes on the prognosis of oral squamous cell carcinoma (OSCC). Study Design: Bone samples were collected from the mandible of 18 patients and submitted for surgical resection for OSCC treatment. Two bone fragments were removed 5 mm from the bone/tumor interface: One was submitted to microcomputedtomography scan (19 mm) and the other to gene expression analysis by quantitative polymerase chain reaction (RANK, RANKL, OPG, and Sclerostin). A total of 8 patients presented with local, regional, or distant recurrence (unfavorable prognostic, n = 8) and 10 patients did not present with recurrence (favorable prognostic, n = 10). Kaplan-Meier estimator, log-rank test, and Cox proportional hazard regression model were performed (after determination of 2 groups of a single cut-point). Results: Intracortical bone surface presented an increased risk (P = .03) of 4.6 for an unfavorable prognostic in the group with higher surface values. Also, lower bone mineraldensity presented increased risk (P = .008) of 10 times and OPG lower expression presented 7.7 fold-risk (P = .07) for unfavorable prognosis. Posterior mandible tumors have lower survival rate (P = .04) compared with anterior ones. Conclusions: Mandible alterations have an effect on the prognosis of patients with OSCC. Increased bone surface, lower mineral density, and lower OPG levels may have an influence for unfavorable prognosis.
TOPOGRAPHIC DISTRIBUTION OF ORAL MUCOSITIS IN PATIENTS WTIH ADVANCED ORAL CANCER TREATED WITH PROPHYLACTIC PHOTOBIOMODULATION. NATALIA RANGEL PALMIER, MARIANA DE PAULIA PAGLIONI, KARINA MORAIS-FARIA, ANA CAROLINA PRADO RIBEIRO, CESAR AUGUSTO MIGLIORATI, ALAN e ROGER SANTOS-SILVA and, THAIS BIANCA BRANDAO Aim: To characterize oral topographies affected by radiation-induced oral mucositis (OM) in patients with oral cancer subjected to prophylactic photobiomodulation (PBM), aiming to improve PBM therapy protocols. Methods: A retrospective clinical study of patients with advanced oral squamous cell carcinoma (OSCC) treated with prophylactic PBM therapy for OM was conducted. The topographic distribution of OM and OM-related pain scores were recorded weekly throughout OSCC treatment. Results: A total of 145 patients with OSCC treated with PBM therapy for the prevention of OM were included in this study. In the last week of cancer treatment, 36 (23%) patients developed severe (grade 3 or 4) OM. In the last day of the radiotherapy (RT), the most affected site of OM was the lateral border of the tongue (64 patients (44.1%). The highest mean value of pain related to OM was 2.69 found in the end of the sixth week of treatment. Conclusions: Although the lateral border of tongue, the buccal mucosa, and the lip mucosa were “topographic hotspots” for radiation-induced OM, the dorsal surface of tongue and the hard palate were also often affected by OM and should be considered relevant topographies for laser probe activation during intraoral PBM sessions in patients with advanced OSCC.
P16 AND MGMT GENE METHYLATION IN CHRONIC MECHANICAL IRRITATION OF THE ORAL MUCOSA. JERONIMO LAZOS, EDUARDO PIEMONTE, RENE PANICO, MARCELA HERNANDEZ RIOS, JESSICA ASTORGA, MABEL BRUNOTTO and, ANA ZARATE Objective: Chronic mechanical irritation (CMI) has been proposed as a risk factor for oral cancer. Epigenetic alterations, particularly methylation, have been mentioned as early events in carcinogenesis, and it has been proposed that CMI could induce them. Thus, the aim of this study is to describe p16 and MGMT gene methylation in a specific CMI lesion: chronic traumatic ulcer (CTU). Study Design: A split-mouth design was used, and 2 samples per individual were taken using cytobrush: the CTU lesion and clinically normal mucosa of a contralateral site. After extracting DNA, p16 protein and MGMT gene methylation status were assessed using quantitative polymerase chain reaction (PCR). The statistical analysis was performed by McNemar test. Results: Twenty-seven patients were studied with a mean age of 59.1 years. CTU evolution time showed an average of 18.4 months. On CTU, methylation of both p16 and MGMT presented a significant difference (P < .0001 and P < .0005, respectively) compared with control site. Conclusions: CMI-affected sites showed consistently more methylation of p16 and MGMT than mucosa devoid of CMI. Since in each case both control and study samples were from the same individual, effects of confounders were reduced.
MARTINS
ABSTRACTS
e149
and,
PABLO
AGUSTIN
Objective: To determine the proteome profile of adenoid cystic carcinoma (AdCC) and polymorphous adenocarcinoma (PAc) and to identify a protein signature useful to differentiate both neoplasms. Study Design: Ten cases of AdCC and 10 cases of PAc were retrospectively retrieved and submitted to laser microdissection for enrichment of neoplastic tissue. The samples were submitted to liquid chromatography-tandem mass spectrometry (LC-MS/MS) and the proteomics data were analyzed using the MaxQuant software. MS/MS spectra were searched against the Human UniProt database, whereas statistical analyses were performed with Perseus software. Results: The LC-MS/MS analysis identified 1957 proteins. Among these, 261 proteins were exclusively identified in AdCC and 106 proteins only in PAc. Clustering analysis of the 394 statistically significant proteins identified in both tumors showed a clear separation of the tumors. A total of 16 proteins were upregulated in each tumor, 6 in AdCC and 10 in PAc, and their cluster analysis also revealed clear differences between both neoplasms. Conclusions: Global proteome can effectively discriminate AdCC and PAc, and the use of a protein signature may represent a useful diagnostic auxiliary to differentiate both tumors in difficult cases. Support: FAPESP 2015/16056-0 and FAPEMIG.
EFFECT OF MANDIBLE ALTERATIONS ON THE PROGNOSIS OF PATIENTS WITH ORAL SQUAMOUS CELL CARCINOMA. ERICA FERNANDA PATRICIO DA SILVA, GUSTAVO DAVI RABELO, CLOVIS ANTONIO LOPES PINTO, VINICIUS FERNANDO CALSAVARA, LUIZ PAULO KOWALSKI and, FABIO ABREU ALVES Objective: This study aimed to evaluate the effect of mandible changes on the prognosis of oral squamous cell carcinoma (OSCC). Study Design: Bone samples were collected from the mandible of 18 patients and submitted for surgical resection for OSCC treatment. Two bone fragments were removed 5 mm from the bone/tumor interface: One was submitted to microcomputedtomography scan (19 mm) and the other to gene expression analysis by quantitative polymerase chain reaction (RANK, RANKL, OPG, and Sclerostin). A total of 8 patients presented with local, regional, or distant recurrence (unfavorable prognostic, n = 8) and 10 patients did not present with recurrence (favorable prognostic, n = 10). Kaplan-Meier estimator, log-rank test, and Cox proportional hazard regression model were performed (after determination of 2 groups of a single cut-point). Results: Intracortical bone surface presented an increased risk (P = .03) of 4.6 for an unfavorable prognostic in the group with higher surface values. Also, lower bone mineraldensity presented increased risk (P = .008) of 10 times and OPG lower expression presented 7.7 fold-risk (P = .07) for unfavorable prognosis. Posterior mandible tumors have lower survival rate (P = .04) compared with anterior ones. Conclusions: Mandible alterations have an effect on the prognosis of patients with OSCC. Increased bone surface, lower mineral density, and lower OPG levels may have an influence for unfavorable prognosis.
TOPOGRAPHIC DISTRIBUTION OF ORAL MUCOSITIS IN PATIENTS WTIH ADVANCED ORAL CANCER TREATED WITH PROPHYLACTIC PHOTOBIOMODULATION. NATALIA RANGEL PALMIER, MARIANA DE PAULIA PAGLIONI, KARINA MORAIS-FARIA, ANA CAROLINA PRADO RIBEIRO, CESAR AUGUSTO MIGLIORATI, ALAN e ROGER SANTOS-SILVA and, THAIS BIANCA BRANDAO Aim: To characterize oral topographies affected by radiation-induced oral mucositis (OM) in patients with oral cancer subjected to prophylactic photobiomodulation (PBM), aiming to improve PBM therapy protocols. Methods: A retrospective clinical study of patients with advanced oral squamous cell carcinoma (OSCC) treated with prophylactic PBM therapy for OM was conducted. The topographic distribution of OM and OM-related pain scores were recorded weekly throughout OSCC treatment. Results: A total of 145 patients with OSCC treated with PBM therapy for the prevention of OM were included in this study. In the last week of cancer treatment, 36 (23%) patients developed severe (grade 3 or 4) OM. In the last day of the radiotherapy (RT), the most affected site of OM was the lateral border of the tongue (64 patients (44.1%). The highest mean value of pain related to OM was 2.69 found in the end of the sixth week of treatment. Conclusions: Although the lateral border of tongue, the buccal mucosa, and the lip mucosa were “topographic hotspots” for radiation-induced OM, the dorsal surface of tongue and the hard palate were also often affected by OM and should be considered relevant topographies for laser probe activation during intraoral PBM sessions in patients with advanced OSCC.
P16 AND MGMT GENE METHYLATION IN CHRONIC MECHANICAL IRRITATION OF THE ORAL MUCOSA. JERONIMO LAZOS, EDUARDO PIEMONTE, RENE PANICO, MARCELA HERNANDEZ RIOS, JESSICA ASTORGA, MABEL BRUNOTTO and, ANA ZARATE Objective: Chronic mechanical irritation (CMI) has been proposed as a risk factor for oral cancer. Epigenetic alterations, particularly methylation, have been mentioned as early events in carcinogenesis, and it has been proposed that CMI could induce them. Thus, the aim of this study is to describe p16 and MGMT gene methylation in a specific CMI lesion: chronic traumatic ulcer (CTU). Study Design: A split-mouth design was used, and 2 samples per individual were taken using cytobrush: the CTU lesion and clinically normal mucosa of a contralateral site. After extracting DNA, p16 protein and MGMT gene methylation status were assessed using quantitative polymerase chain reaction (PCR). The statistical analysis was performed by McNemar test. Results: Twenty-seven patients were studied with a mean age of 59.1 years. CTU evolution time showed an average of 18.4 months. On CTU, methylation of both p16 and MGMT presented a significant difference (P < .0001 and P < .0005, respectively) compared with control site. Conclusions: CMI-affected sites showed consistently more methylation of p16 and MGMT than mucosa devoid of CMI. Since in each case both control and study samples were from the same individual, effects of confounders were reduced.