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Two new agarics from South India
III
Short Communications In Europe, according to Gaumann (19S9), B. benekenii and B. ramosus carry telia of a race (P. symphyti-bromorum F. Muller) alternating with Pulmonaria and Symphytum, hosts widely cultivated in Britain. No rust sori were detected on British sheets of B. carinatus Hook. & Am., B. inermis Leyss, B. rigidus Roth, B. tectorum L. or B. unioloides H.B.K., alien species which bear the fungus elsewhere. REFERENCES
DE LANGHE, J. E. et al. (1983). Nouvelle Flore de la Belgique, edn 3. Meise. GAUMANN, E. (1959). Die Rostpilze Mitteleuropas. Bei/rage zur Kryptogamenflora der Schweiz XII. HUBBARD, C. E. (1968) Grasses. Harmondsworth, UK: Pelican Books. WHITE, J. W. (1912). The Flora of Bristol. Bristol, U.K.: Chatford House (Reprint, 1972). WILSON, M, & HENDERSON, D. M. (1966). British Rust Fungi. Cambridge, U.K.: Cambridge University Press.
CLAPHAM, A. R., TUTIN, T. G. & WARBURG, E. F. (1952). Flora of the British Isles. Cambridge, UK: Cambridge University Press.
Two new agarics from south India K.NATARA]AN AND K.B.PURUSHOTHAMA c.A.S. in Botany, University of Madras, Guindy Campus, Madras 600 025, India
Two new agarics from south India. Mycological Research 92 (1): 111-113 (1989). Agrocybe musicola sp. nov. and fccilia excentrica sp. nov. are described from South India.
Key words: Agrocybe musicola, fccilia excentrica, Musa, Indian agarics. Two new species, Agrocybe musicola sp. nov. and Eccilia excentrica sp. nov. from southern India are described and illustrated. The specimens are deposited in the herbarium of the Botany Laboratory, University of Madras. The colour terminology used is that of Kornerup & Wanscher (1967).
Agrocybe musicola Natarajan & Purushothama, sp. nov. (Fig. lA-E) Pileus 1-1'5 em latus. e convexo planus, pallido-aurantiacus vel griseo-luteus, squamulis minutis, interdum ad marginem siccus. Lamellae adnatae, albo-aurantiacae et griseo-aurantiacae. Stipes centralis, 1'5-3'5 X 0·2....{ )-3 em, cylindricus ad basim subbulbosus et strigosus, albidus, glaber; annulus fragilis, evanescens. Sporae 13-16 x 7-9 ~m, Q = 1'7-2'1, ellipsoideae, crassitunicatae, subfuscae; poro germinativo absentio. Basidia 25-34 x 10--12 ~m, clavata, 4-sporigera. Cheilocystidia 30-40 x 12-30 ~m, clavata vel lageniformia, tenuitunicata. Pleurocystidia nulla. Trama hymenophoralis regularis. Cuticula pilei cellularis.
In pseudocaule putrescenti Musae, Guthigar (Sullia, SK), Kamataka, India, 13 Sept. 1984, K. B. Purushothama, Herb. MUBL 3042, holotypus.
Pileus l-l'S cm in diam, convex becoming applanate, surface light orange (SA4) to greyish yellow (4B3), minutely squamulose to almost glabrous; margin thin, often splitting. Lamellae adnate, orange white (SA2) to greyish orange (SB3), crowded, with lamellulae. Stipe central, l'5-3'S x 0'2-0'3 cm, cylindrical with slightly bulbous base, hollow; surface white, glabrous, minutely striate, initially with partial velar remnants over middle of the stipe. Spore print orange grey (5B2).
Basidiospores ellipsoid, 13-16 x 7-9 ~m, Q = 1'7-2'1, light brown, darkening in alkali, inamyloid, non-cyanophilous, thick-walled (-l'S ~m), 2-layered, germ-pore absent, with few small refractive guttules. Basidia clavate, 25-34 x 10-12 ~m, tetrasporic; sterigmata slender, 2-3 J..lm long. Lamella-edge heteromorphous. Cheilocystidia present, clavate to lageniform, 30-40 x 12-30 ~m, hyaline, thin-walled. Pleurocystidia absent. Hymenophoral trama regular, hyphae 2-S ~m diam, with slightly thick wall (up to 0'75 ~m). Context thin, 1-2 mm thick, concolorous with pileus surface; hyphae 3-9 ~m diam, with darker contents. Pileus surface epithelial, elements pyriform to subglobose, rarely clavate, pedicellate, 21-36 x 13-28 ~m, wall often collapsed; without pileocystidia. Caulocystidia absent. Clamp-connexions absent. Additional specimen examined: on pseudostem of Musa, solitary, in Ajjavara (Sullia, S.K.), Kamataka, India, 19 Sept. 1984, K. B. Purushothama, Herb. MUBL No. 3043.
The characteristic features of this species are the small, pale basidiocarp, with large spores lacking a germ-pore. Smaller, paler basidiocarps, a larger spore size, and the absence of pleurocystidia distinguish this ~pecies from other species lacking germ-pores such as A. erebia (Fr.) Kuhner, A. lazoi Singer and A. aporata Watl. & Abraham. Eccilia excentrica Natarajan & Purushothama, sp. nov. (Fig. 2 A-D) Pileus 3-3'8 em latus, plano-convexus, postremo depressus, subaurantiacus vel subalbus, glaber. Lamellae decurrentes, subaurantiacae. Stipes excentricus, 1-1'5 x O' 2-D' 3 em, infra aHenuatus, cavus, albidus, glaber. Sporae 5-6 x 4-5 \lm, Q = 1'2-1'5, isodiametrico-obtusae, MYC 92
Short Communications
112
Fig. 1. Agrocybe musicola. A, Habit, x 3; B , basidia; C basidiospores; D, cheilocystidia; E, epithelial structures.
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• Fig. 2. Eccilia ercentrica. A, Habit, x 2; B, basidia; C basidiospores; D, caulocystidia.
B
20 11m
2
113
Short Communications roseae, crassitunicatae. Basidia 15-23 x 5-7 Ilm, davata, 4-sporigera. Acies lamellarum fertilis; cystidia nulla. Trama hymenophoralis regularis. Cuticula pilei ex hyphis repentibus, 2-4 Ilm latae. Caulocystidia hyphoidea, 22-35 x 4-5 Ilm, tenuitunicata. In lignis defossis, Guthigar (Sullia, SK), Kamataka, India, 14 Sept. 1984, K. B. Purushothama, Herb. MUBL 3060, holotypus.
Pileus 3-3'8 cm in diam, plano-convex becoming depressed where the excentric stipe originates; surface orange white (6A2), becoming dull white, glabrous; margin entire, inrolled. Lamellae decurrent, thin, orange white (5A2), crowded; with lamellulae. Stipe excentric, 1-1'5 X O'2-D'3 cm, attenuate towards base, hollow; surface white, glabrous. Spore print pinkish white (8A2). Basidiospores isodiametric, 5-6 x 4'5 ~m, Q = 1'2-1'5, regularly faceted, pinkish, inamyloid, with 2layered wall and prominent refractive guttules. Basidia clavate, 15-23 x 5-7 ~m, tetrasporic; sterigmata slender, 2-3 ~m long. Lamella-edge fertile. Cheilocystidia and pleurocystidia absent. Hymenophoral trama regular; hyphae 3-11 ~m diam, thin-walled: subhymenium cellular. Context thin, up to 2 mm thick, white; hyphae 2-12 ~m diam. Pileus surface a cutis, hyphae 2-4 ~m diam, interwoven, not incrusted, Stipe surface hyphae project to form hyphoid caulocystidia, 22-35 x 4-5 ~m, thin-walled. Clamp-connexions absent.
Additional specimen examined: on buried wood, in group, in Jodpala, Coorg Distr. Kamataka, India, 24 Sept. 1984, K. B. Purushothama, Herb. MUBL No. 3061.
This fungus is placed in the genus Eccilia (Fr.) Kummer owing to the presence of decurrent lamellae and relatively large basidiocarps. The basidiocarp and stipe dimensions preclude placement in Claudopus Gillet, which comprises a few minute species with pileus generally less than 1 cm in diam and either sessile or with a rudimentary stipe. The excentric stipe and smaller spores differentiate this species from other species of Eccilia. One of us (K. B. P) is grateful to the University Grants Commission, New Delhi for the award of a Junior Research Fellowship. Thanks are due to Dr D. N. Pegler, Royal Botanic Gardens, Kew for his valuable comments.
REFERENCES KORNERUP, A. & WANSCHER, J. H. (1967). Methuen Handbook of Colour, 2nd edn. London: Methuen.
Coprophilous fungi from Spain: Klasterskya coronata sp. nov. M. VALLDOSERA AND J. GUARRO Unitat de Biolog{ i Microbiologla, Universitat de Barcelona, FacuItat de Medicina, 43201 Reus, Tarragona, Spain
Coprophilous fungi from Spain: Klasterskya coranata sp. nov. Mycological Research 92 (1): 113-116 (1989). Klasterskya coronata sp. nov., collected on horse dung from Girona, Catalonia, Spain, is described and illustrated. Pyxidiophora crenata is formally placed in Klasterskya.
Key words: Klasterskya coronata, Klasterskya crenata, Pyxidiophora crenata, Coprophilous fungi, Spanish ascomycetes. In our current work on coprophilous fungi from Spain we have recently observed flask-shaped dark ascomata on horse dung. On cursory examination they could be ascribed to Pyxidiophora Bref. & TaveL on the basis of neck length and presence of 1septate hyaline ascospores. However, after a more detailed study and in the light of Minter's contribution of 1983, we are now inclined to believe that Klasterskya Petr. is a more correct assignation, especially because of the dark peridium and the characteristic ascospore morphology. The aim of this paper is to present a description of this fungus based on ascomata growing on the natural substrate, as we have failed in its pure culture, and to discuss its taxonomy.
Fig. 1. Klasterskya coronata. A, Ascoma; B, Upper part of the neck; C, Cells of the peridium; D, Ascus and ascospores.
"
:.; :
Klasterskya coronata Valldosera & Guarro, sp. nov. (Figs 1-8)
Ascomata partim immersa et partim superficialia, sine stromate, dispersa .vel aggregata, subglobosa vel obpyriformia, ostiolata,
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B
C
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IOj.lm
Short Communications In Europe, according to Gaumann (19S9), B. benekenii and B. ramosus carry telia of a race (P. symphyti-bromorum F. Muller) alternating with Pulmonaria and Symphytum, hosts widely cultivated in Britain. No rust sori were detected on British sheets of B. carinatus Hook. & Am., B. inermis Leyss, B. rigidus Roth, B. tectorum L. or B. unioloides H.B.K., alien species which bear the fungus elsewhere. REFERENCES
DE LANGHE, J. E. et al. (1983). Nouvelle Flore de la Belgique, edn 3. Meise. GAUMANN, E. (1959). Die Rostpilze Mitteleuropas. Bei/rage zur Kryptogamenflora der Schweiz XII. HUBBARD, C. E. (1968) Grasses. Harmondsworth, UK: Pelican Books. WHITE, J. W. (1912). The Flora of Bristol. Bristol, U.K.: Chatford House (Reprint, 1972). WILSON, M, & HENDERSON, D. M. (1966). British Rust Fungi. Cambridge, U.K.: Cambridge University Press.
CLAPHAM, A. R., TUTIN, T. G. & WARBURG, E. F. (1952). Flora of the British Isles. Cambridge, UK: Cambridge University Press.
Two new agarics from south India K.NATARA]AN AND K.B.PURUSHOTHAMA c.A.S. in Botany, University of Madras, Guindy Campus, Madras 600 025, India
Two new agarics from south India. Mycological Research 92 (1): 111-113 (1989). Agrocybe musicola sp. nov. and fccilia excentrica sp. nov. are described from South India.
Key words: Agrocybe musicola, fccilia excentrica, Musa, Indian agarics. Two new species, Agrocybe musicola sp. nov. and Eccilia excentrica sp. nov. from southern India are described and illustrated. The specimens are deposited in the herbarium of the Botany Laboratory, University of Madras. The colour terminology used is that of Kornerup & Wanscher (1967).
Agrocybe musicola Natarajan & Purushothama, sp. nov. (Fig. lA-E) Pileus 1-1'5 em latus. e convexo planus, pallido-aurantiacus vel griseo-luteus, squamulis minutis, interdum ad marginem siccus. Lamellae adnatae, albo-aurantiacae et griseo-aurantiacae. Stipes centralis, 1'5-3'5 X 0·2....{ )-3 em, cylindricus ad basim subbulbosus et strigosus, albidus, glaber; annulus fragilis, evanescens. Sporae 13-16 x 7-9 ~m, Q = 1'7-2'1, ellipsoideae, crassitunicatae, subfuscae; poro germinativo absentio. Basidia 25-34 x 10--12 ~m, clavata, 4-sporigera. Cheilocystidia 30-40 x 12-30 ~m, clavata vel lageniformia, tenuitunicata. Pleurocystidia nulla. Trama hymenophoralis regularis. Cuticula pilei cellularis.
In pseudocaule putrescenti Musae, Guthigar (Sullia, SK), Kamataka, India, 13 Sept. 1984, K. B. Purushothama, Herb. MUBL 3042, holotypus.
Pileus l-l'S cm in diam, convex becoming applanate, surface light orange (SA4) to greyish yellow (4B3), minutely squamulose to almost glabrous; margin thin, often splitting. Lamellae adnate, orange white (SA2) to greyish orange (SB3), crowded, with lamellulae. Stipe central, l'5-3'S x 0'2-0'3 cm, cylindrical with slightly bulbous base, hollow; surface white, glabrous, minutely striate, initially with partial velar remnants over middle of the stipe. Spore print orange grey (5B2).
Basidiospores ellipsoid, 13-16 x 7-9 ~m, Q = 1'7-2'1, light brown, darkening in alkali, inamyloid, non-cyanophilous, thick-walled (-l'S ~m), 2-layered, germ-pore absent, with few small refractive guttules. Basidia clavate, 25-34 x 10-12 ~m, tetrasporic; sterigmata slender, 2-3 J..lm long. Lamella-edge heteromorphous. Cheilocystidia present, clavate to lageniform, 30-40 x 12-30 ~m, hyaline, thin-walled. Pleurocystidia absent. Hymenophoral trama regular, hyphae 2-S ~m diam, with slightly thick wall (up to 0'75 ~m). Context thin, 1-2 mm thick, concolorous with pileus surface; hyphae 3-9 ~m diam, with darker contents. Pileus surface epithelial, elements pyriform to subglobose, rarely clavate, pedicellate, 21-36 x 13-28 ~m, wall often collapsed; without pileocystidia. Caulocystidia absent. Clamp-connexions absent. Additional specimen examined: on pseudostem of Musa, solitary, in Ajjavara (Sullia, S.K.), Kamataka, India, 19 Sept. 1984, K. B. Purushothama, Herb. MUBL No. 3043.
The characteristic features of this species are the small, pale basidiocarp, with large spores lacking a germ-pore. Smaller, paler basidiocarps, a larger spore size, and the absence of pleurocystidia distinguish this ~pecies from other species lacking germ-pores such as A. erebia (Fr.) Kuhner, A. lazoi Singer and A. aporata Watl. & Abraham. Eccilia excentrica Natarajan & Purushothama, sp. nov. (Fig. 2 A-D) Pileus 3-3'8 em latus, plano-convexus, postremo depressus, subaurantiacus vel subalbus, glaber. Lamellae decurrentes, subaurantiacae. Stipes excentricus, 1-1'5 x O' 2-D' 3 em, infra aHenuatus, cavus, albidus, glaber. Sporae 5-6 x 4-5 \lm, Q = 1'2-1'5, isodiametrico-obtusae, MYC 92
Short Communications
112
Fig. 1. Agrocybe musicola. A, Habit, x 3; B , basidia; C basidiospores; D, cheilocystidia; E, epithelial structures.
.:.. (ffIj)
.~. . Q .:.,;::. "
"
: ~ .......
. . . ::.. . :' .
Pi
f£jI
.
":.,
,
:"",
. .:.
······.t<··· .
:.:.:. <:~
C.'
:\
.
'
• Fig. 2. Eccilia ercentrica. A, Habit, x 2; B, basidia; C basidiospores; D, caulocystidia.
B
20 11m
2
113
Short Communications roseae, crassitunicatae. Basidia 15-23 x 5-7 Ilm, davata, 4-sporigera. Acies lamellarum fertilis; cystidia nulla. Trama hymenophoralis regularis. Cuticula pilei ex hyphis repentibus, 2-4 Ilm latae. Caulocystidia hyphoidea, 22-35 x 4-5 Ilm, tenuitunicata. In lignis defossis, Guthigar (Sullia, SK), Kamataka, India, 14 Sept. 1984, K. B. Purushothama, Herb. MUBL 3060, holotypus.
Pileus 3-3'8 cm in diam, plano-convex becoming depressed where the excentric stipe originates; surface orange white (6A2), becoming dull white, glabrous; margin entire, inrolled. Lamellae decurrent, thin, orange white (5A2), crowded; with lamellulae. Stipe excentric, 1-1'5 X O'2-D'3 cm, attenuate towards base, hollow; surface white, glabrous. Spore print pinkish white (8A2). Basidiospores isodiametric, 5-6 x 4'5 ~m, Q = 1'2-1'5, regularly faceted, pinkish, inamyloid, with 2layered wall and prominent refractive guttules. Basidia clavate, 15-23 x 5-7 ~m, tetrasporic; sterigmata slender, 2-3 ~m long. Lamella-edge fertile. Cheilocystidia and pleurocystidia absent. Hymenophoral trama regular; hyphae 3-11 ~m diam, thin-walled: subhymenium cellular. Context thin, up to 2 mm thick, white; hyphae 2-12 ~m diam. Pileus surface a cutis, hyphae 2-4 ~m diam, interwoven, not incrusted, Stipe surface hyphae project to form hyphoid caulocystidia, 22-35 x 4-5 ~m, thin-walled. Clamp-connexions absent.
Additional specimen examined: on buried wood, in group, in Jodpala, Coorg Distr. Kamataka, India, 24 Sept. 1984, K. B. Purushothama, Herb. MUBL No. 3061.
This fungus is placed in the genus Eccilia (Fr.) Kummer owing to the presence of decurrent lamellae and relatively large basidiocarps. The basidiocarp and stipe dimensions preclude placement in Claudopus Gillet, which comprises a few minute species with pileus generally less than 1 cm in diam and either sessile or with a rudimentary stipe. The excentric stipe and smaller spores differentiate this species from other species of Eccilia. One of us (K. B. P) is grateful to the University Grants Commission, New Delhi for the award of a Junior Research Fellowship. Thanks are due to Dr D. N. Pegler, Royal Botanic Gardens, Kew for his valuable comments.
REFERENCES KORNERUP, A. & WANSCHER, J. H. (1967). Methuen Handbook of Colour, 2nd edn. London: Methuen.
Coprophilous fungi from Spain: Klasterskya coronata sp. nov. M. VALLDOSERA AND J. GUARRO Unitat de Biolog{ i Microbiologla, Universitat de Barcelona, FacuItat de Medicina, 43201 Reus, Tarragona, Spain
Coprophilous fungi from Spain: Klasterskya coranata sp. nov. Mycological Research 92 (1): 113-116 (1989). Klasterskya coronata sp. nov., collected on horse dung from Girona, Catalonia, Spain, is described and illustrated. Pyxidiophora crenata is formally placed in Klasterskya.
Key words: Klasterskya coronata, Klasterskya crenata, Pyxidiophora crenata, Coprophilous fungi, Spanish ascomycetes. In our current work on coprophilous fungi from Spain we have recently observed flask-shaped dark ascomata on horse dung. On cursory examination they could be ascribed to Pyxidiophora Bref. & TaveL on the basis of neck length and presence of 1septate hyaline ascospores. However, after a more detailed study and in the light of Minter's contribution of 1983, we are now inclined to believe that Klasterskya Petr. is a more correct assignation, especially because of the dark peridium and the characteristic ascospore morphology. The aim of this paper is to present a description of this fungus based on ascomata growing on the natural substrate, as we have failed in its pure culture, and to discuss its taxonomy.
Fig. 1. Klasterskya coronata. A, Ascoma; B, Upper part of the neck; C, Cells of the peridium; D, Ascus and ascospores.
"
:.; :
Klasterskya coronata Valldosera & Guarro, sp. nov. (Figs 1-8)
Ascomata partim immersa et partim superficialia, sine stromate, dispersa .vel aggregata, subglobosa vel obpyriformia, ostiolata,
.,:
\
~ ~. ;
.:
t . ,;.:.
B
C
1----1
Etyro.: Referring the apex of the neck, similar to a crown, corona (L).
"
A
1----1
lOOj.lm
IOj.lm