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W07-P-009 Decreased size of the HDL-C particles characterizes HDL subspecies in low HDL-C family members
Workshops W7 Genetic determinants of HDL metabolism
28
normal. The analysis of serum cholesteryl esters composition revealed oleic acid as the largest fraction. This is the first LCAT mutation described in the Slavic population.
I W07-P-007 I P O L Y M O R P H I S M S O F THE C H O L E S T E R Y L ESTER TRANSFER PROTEIN GENE AND SEVERITY OF CORONARY STENOSIS ESTIMATED BY GENSINI AND JEOPARDY SCORES D. Kolovou 1, K. Anagnosto~oulou 2, P. Klyofyllis I , K. Salpe a2, N. Pilatis 1, N. Yiannakouris~, D. Zarkalis 4, D. Cokkinos 1. ~lst Cardiology
Departmen~ Onassis Cardiac Surgery Center, Athens, Greece; 2Molecular Immunology laboratory, Onassis Cardiac Surgery Center, Athens, Greece; 3Harokopio University, Athens, Greece; 43rd Cardiac Surgery Departmen~ Onassis Cardiac Surgery Center, Athens, Greece Objective: Cholesteryl ester transfer protein (CETP) gene is a candidate for studying the susceptibility to coronary artery disease (CHD), as it facilitates the exchange of triglycerides and cholesteryl esters between lipoprotein particles. The aim of the study was to determine any association between two CETP polymorphisms (TaqIB, I405V) and severity of coronary stenosis. Methods: The severity of coronary steuosis was estimeted by both Gensini (GS) and Jeopardy (JS) scores in 124 individuals (mean age 62+ 10) (8% postmenopausal women) who underwent coronary angiography. The CETP polymorphisms were detected by using polymerase chain reaction and restricted fragment length polymorphism analysis. Remits: The correlation between GS and I405V polymorphism was found, p=0.046. The GS was lower in II compared to IV subjects [81(95% CI 57-105) vs 122(95% CI 87-155) p=0.032]. However, the highest score was found in VV subjects [147 (95% CI 172-222)] but they consisted a small group of patients. No correlation was found between GS and TaqIB polymorphism, as well as between JS and any of the CETP polymorphisms. Conclusions: It seems that homozygotes of CETP I405V have less sever coronary stenosis compared to heterozygotes. However, further prospective investigations in a large population are required to confirm these findings.
I WO7-P-O08 I A R E I i
SERUM HDL-C LEVELS OF T U R K I S H P E O P L E REALLY LOW?
A. Tunfkale ] , S. Aran 2, S. Tavsanoglu 3, A. Dirican 4 .]Department of
Internal Medicine; 2Department of Family Medicine; 3Department of Cardiology; 4Department of Biostatistics, Cerrahpasa Medical School Istanbul University, Istanbul, Turkey Objective: Recent studies indicate that low HDL-C (high density lipoprotein-cholesterol) level is an important risk factor for atherosclerosis. Previous studies disclosed that HDL-C levels of the Turkish population are low. The aim of this study to confirm the validity of this information. Methods: The study was done on patients who applied to the Cerrahpasa medical School with various complaints. The data was collected by analyzing retrospectively the electronic patient records. Those patients who were on lipid lowering medications and those who had a chronic systemic disease were excluded. A total of 14,230 patients (5370 men and 8860 women) were included in the study. Mean age was 52 4- 15 years (Range 20-95 years). Remits: The plasma lipid levels are shown in Table 1. Serum levels of HDL-C in men and women were higher than the results of the former studies. Table 1. Plasma lipid levels in males and females Total (n=14230) Male (n=5370) Female (n=8860) Total cholesterol (mg/dL) 197 ± 35 193 ± 35 199 ± 35 Triglycerides (mg/dL) 124 4- 52 131 ± 53 120 ± 51 LDL- C (mg/dL) 129 4- 31 129 ± 31 130 ± 31 HDL-C (mg/dL) 44 ± 10 40 ± 9 47 4- 10 mg/dL=milligrams per deciliter, LDL-C=Iow density lipeprotein-cholesteml The distribution of HDL-C levels according to sex is shown in Table 2. HDL-C levels were higher than 40 mg/dL in 50% of men and in 70% of woIn~u.
Conelnsions: In our study the HDL-C levels in both sexes have been found to be 2 mg/dL higher than the previous studies. These findings suggest that the HDL-C levels may not be low in the Turkish population.
Table 2. The distribution of HDL-C levels according to sex HDL-C
Male (n=5370)
Female (n=8860)
13.6 17.3 22.6 18.0 28.4
4.9 9.0 15.1 16.3 54.8
< 30 mg/dL (%) 31-35 mg/dL (%) 36-40 mg/dL (%) 41-45 mg/dL (%) > 46 mg/dL (%)
Therefore the data indicating low HDL-C levels has to be reevaluated and large scale population screening studies have to be done. I
W07-P-009 I DECREASED SIZE OF THE HDL-C PARTICLES C H A R A C T E R I Z E S HDL SUBSPECIES IN L O W HDL-C FAMILY M E M B E R S I
H. Watanabe 1, A. Soro-Paavonen 1, S. Sodedund 1, M. Jauhiainen 2, M.-R. Taskinen 1. 1Department of Medicine, Division of Cardiology,
University of Helsinki, Helsinki, Finland; 2Department of Molecular Medicine, National Public Health Institute, Helsinki, Finland Objective: To examine HDL subclass distribution in Finnish low HDL families by separating the HDL subclasses by density, size and apoprotein composition in affected and unaffected low HDL family members. Methods: The integrated HDL diameter and percentage of HDL2b, 2a, 3a, 3b, and 3c were measured in 73 subjects with familial low HDL family using gradient gel electrophoresis. The family members were categorized as affected (n=39) or unaffected (n=34) according to the 10th age-gender specific HDL-C percentile. Results: In affected members, age, BMI, sBP, waist circumference, TG, LDL-C, apoB, fasting glucose were significantly higher and HDL-C, apoA1 and apoA2 were significantly lower than in unaffected members. The integrated HDL diameter was markedly smaller in affected members than in unaffected members (90.1 + 2.98/~ vs. 94.8 4- 3.92/~, p<0.0001). In affected members the greatest difference was in HDL2b that was reduced by about 50% compared with unaffected members. Univariate regression analysis demonstrated that HDL-C, apoA1, waist circumference, BMI, TG, apoB, gender, LDL-C, and age correlated with the integrated HDL diameter (r=-0.80, 0.66, -0.64, -0.60, -0.53, -0.49, 0.48, -0.34, -0.30). TG (mmol/l) Affected 1.54 ± 0.67 Unaffected 0.95±0.39 (mean -1-S.D.)
HDL-C (mmol/l) 0.96 ± 0.23 1.59±0.47
LDL-C (mmoFl) 3.02 ± 0.86 2.81±0.90
ApoA1 ApoB (mg/dl) (mg/dl) 112 ± 13.9 103.8± 25.5 148±23.5 86.6±27.0
Conclusions: Smaller and potentially less protective HDL particles predominate in the fasting serum of the affected low HDL family members.
l WO7-P-010 I C H A R A C T E R I S T I C S O F THE HDL-C PARTICLE SIZE IN L O W HDL-C FAMILY M E M B E R S I
H. Watanabe, A. Soro-Paavonen, M.-R. Taskinen. Department of Medicine,
Division of Cardiology, University of Helsinki, Finland Objective: To examine the factors contributing to HDL subclass distribution in Finish low HDL families by separating the HDL subclasses by density, size and apoprotein composition in affected and unaffected low HDL family members. Methods: HDL particle size was measured 73 subjects with familial low HDL family using gradient gel electrophoresis. The family members were categorized as affected or unaffected according to the 10th age-gender specific HDL-C percentile. Results: There were 39 subjects in affected family members and 34 subjects in unaffected family members. In affected family members, age, body mass index, sBP, waist circumference, TG, LDL-C, apoB, fasting glucose were significant higher and HDL-C, apoA1 and apoA2 were significant lower than that of unaffected family members. HDL particle size was smaller in affected family members than in unaffected family members (90.1 4- 2.98/~ vs. 94.8 4- 3.92/~, p<0.0001). In affected family members the HDL-C2b was almost 50% reduced compared with unaffected family members. Multivariate regression analysis demonstrated that age, gender, body mass index, waist circumference, TG, HDL-C, LDL-C, apoA1 and apoB have independent contribution to HDL-C particle size.
75th EAS Congress, 23-26 April 2005, Prague, Czech Republic
28
normal. The analysis of serum cholesteryl esters composition revealed oleic acid as the largest fraction. This is the first LCAT mutation described in the Slavic population.
I W07-P-007 I P O L Y M O R P H I S M S O F THE C H O L E S T E R Y L ESTER TRANSFER PROTEIN GENE AND SEVERITY OF CORONARY STENOSIS ESTIMATED BY GENSINI AND JEOPARDY SCORES D. Kolovou 1, K. Anagnosto~oulou 2, P. Klyofyllis I , K. Salpe a2, N. Pilatis 1, N. Yiannakouris~, D. Zarkalis 4, D. Cokkinos 1. ~lst Cardiology
Departmen~ Onassis Cardiac Surgery Center, Athens, Greece; 2Molecular Immunology laboratory, Onassis Cardiac Surgery Center, Athens, Greece; 3Harokopio University, Athens, Greece; 43rd Cardiac Surgery Departmen~ Onassis Cardiac Surgery Center, Athens, Greece Objective: Cholesteryl ester transfer protein (CETP) gene is a candidate for studying the susceptibility to coronary artery disease (CHD), as it facilitates the exchange of triglycerides and cholesteryl esters between lipoprotein particles. The aim of the study was to determine any association between two CETP polymorphisms (TaqIB, I405V) and severity of coronary stenosis. Methods: The severity of coronary steuosis was estimeted by both Gensini (GS) and Jeopardy (JS) scores in 124 individuals (mean age 62+ 10) (8% postmenopausal women) who underwent coronary angiography. The CETP polymorphisms were detected by using polymerase chain reaction and restricted fragment length polymorphism analysis. Remits: The correlation between GS and I405V polymorphism was found, p=0.046. The GS was lower in II compared to IV subjects [81(95% CI 57-105) vs 122(95% CI 87-155) p=0.032]. However, the highest score was found in VV subjects [147 (95% CI 172-222)] but they consisted a small group of patients. No correlation was found between GS and TaqIB polymorphism, as well as between JS and any of the CETP polymorphisms. Conclusions: It seems that homozygotes of CETP I405V have less sever coronary stenosis compared to heterozygotes. However, further prospective investigations in a large population are required to confirm these findings.
I WO7-P-O08 I A R E I i
SERUM HDL-C LEVELS OF T U R K I S H P E O P L E REALLY LOW?
A. Tunfkale ] , S. Aran 2, S. Tavsanoglu 3, A. Dirican 4 .]Department of
Internal Medicine; 2Department of Family Medicine; 3Department of Cardiology; 4Department of Biostatistics, Cerrahpasa Medical School Istanbul University, Istanbul, Turkey Objective: Recent studies indicate that low HDL-C (high density lipoprotein-cholesterol) level is an important risk factor for atherosclerosis. Previous studies disclosed that HDL-C levels of the Turkish population are low. The aim of this study to confirm the validity of this information. Methods: The study was done on patients who applied to the Cerrahpasa medical School with various complaints. The data was collected by analyzing retrospectively the electronic patient records. Those patients who were on lipid lowering medications and those who had a chronic systemic disease were excluded. A total of 14,230 patients (5370 men and 8860 women) were included in the study. Mean age was 52 4- 15 years (Range 20-95 years). Remits: The plasma lipid levels are shown in Table 1. Serum levels of HDL-C in men and women were higher than the results of the former studies. Table 1. Plasma lipid levels in males and females Total (n=14230) Male (n=5370) Female (n=8860) Total cholesterol (mg/dL) 197 ± 35 193 ± 35 199 ± 35 Triglycerides (mg/dL) 124 4- 52 131 ± 53 120 ± 51 LDL- C (mg/dL) 129 4- 31 129 ± 31 130 ± 31 HDL-C (mg/dL) 44 ± 10 40 ± 9 47 4- 10 mg/dL=milligrams per deciliter, LDL-C=Iow density lipeprotein-cholesteml The distribution of HDL-C levels according to sex is shown in Table 2. HDL-C levels were higher than 40 mg/dL in 50% of men and in 70% of woIn~u.
Conelnsions: In our study the HDL-C levels in both sexes have been found to be 2 mg/dL higher than the previous studies. These findings suggest that the HDL-C levels may not be low in the Turkish population.
Table 2. The distribution of HDL-C levels according to sex HDL-C
Male (n=5370)
Female (n=8860)
13.6 17.3 22.6 18.0 28.4
4.9 9.0 15.1 16.3 54.8
< 30 mg/dL (%) 31-35 mg/dL (%) 36-40 mg/dL (%) 41-45 mg/dL (%) > 46 mg/dL (%)
Therefore the data indicating low HDL-C levels has to be reevaluated and large scale population screening studies have to be done. I
W07-P-009 I DECREASED SIZE OF THE HDL-C PARTICLES C H A R A C T E R I Z E S HDL SUBSPECIES IN L O W HDL-C FAMILY M E M B E R S I
H. Watanabe 1, A. Soro-Paavonen 1, S. Sodedund 1, M. Jauhiainen 2, M.-R. Taskinen 1. 1Department of Medicine, Division of Cardiology,
University of Helsinki, Helsinki, Finland; 2Department of Molecular Medicine, National Public Health Institute, Helsinki, Finland Objective: To examine HDL subclass distribution in Finnish low HDL families by separating the HDL subclasses by density, size and apoprotein composition in affected and unaffected low HDL family members. Methods: The integrated HDL diameter and percentage of HDL2b, 2a, 3a, 3b, and 3c were measured in 73 subjects with familial low HDL family using gradient gel electrophoresis. The family members were categorized as affected (n=39) or unaffected (n=34) according to the 10th age-gender specific HDL-C percentile. Results: In affected members, age, BMI, sBP, waist circumference, TG, LDL-C, apoB, fasting glucose were significantly higher and HDL-C, apoA1 and apoA2 were significantly lower than in unaffected members. The integrated HDL diameter was markedly smaller in affected members than in unaffected members (90.1 + 2.98/~ vs. 94.8 4- 3.92/~, p<0.0001). In affected members the greatest difference was in HDL2b that was reduced by about 50% compared with unaffected members. Univariate regression analysis demonstrated that HDL-C, apoA1, waist circumference, BMI, TG, apoB, gender, LDL-C, and age correlated with the integrated HDL diameter (r=-0.80, 0.66, -0.64, -0.60, -0.53, -0.49, 0.48, -0.34, -0.30). TG (mmol/l) Affected 1.54 ± 0.67 Unaffected 0.95±0.39 (mean -1-S.D.)
HDL-C (mmol/l) 0.96 ± 0.23 1.59±0.47
LDL-C (mmoFl) 3.02 ± 0.86 2.81±0.90
ApoA1 ApoB (mg/dl) (mg/dl) 112 ± 13.9 103.8± 25.5 148±23.5 86.6±27.0
Conclusions: Smaller and potentially less protective HDL particles predominate in the fasting serum of the affected low HDL family members.
l WO7-P-010 I C H A R A C T E R I S T I C S O F THE HDL-C PARTICLE SIZE IN L O W HDL-C FAMILY M E M B E R S I
H. Watanabe, A. Soro-Paavonen, M.-R. Taskinen. Department of Medicine,
Division of Cardiology, University of Helsinki, Finland Objective: To examine the factors contributing to HDL subclass distribution in Finish low HDL families by separating the HDL subclasses by density, size and apoprotein composition in affected and unaffected low HDL family members. Methods: HDL particle size was measured 73 subjects with familial low HDL family using gradient gel electrophoresis. The family members were categorized as affected or unaffected according to the 10th age-gender specific HDL-C percentile. Results: There were 39 subjects in affected family members and 34 subjects in unaffected family members. In affected family members, age, body mass index, sBP, waist circumference, TG, LDL-C, apoB, fasting glucose were significant higher and HDL-C, apoA1 and apoA2 were significant lower than that of unaffected family members. HDL particle size was smaller in affected family members than in unaffected family members (90.1 4- 2.98/~ vs. 94.8 4- 3.92/~, p<0.0001). In affected family members the HDL-C2b was almost 50% reduced compared with unaffected family members. Multivariate regression analysis demonstrated that age, gender, body mass index, waist circumference, TG, HDL-C, LDL-C, apoA1 and apoB have independent contribution to HDL-C particle size.
75th EAS Congress, 23-26 April 2005, Prague, Czech Republic