- Email: [email protected]
4889818 Purified thermostable enzyme
632
PATENT ABSTRACTS glass, aminated zeolite, or water insoluble crosslinked chitosen,
area by at least 20~o. The analytical element exhibits high sensitivity and high reproducibility.
4888288 VESICLES RESISTANT TO ENZYME LYSIS AND USE THEREOF IN AN ENZYME ASSAY Daniel Wagner assigned to Becton Dickinson and Company Liposomes are formed from compounds ineluding a phosphonate or phosphinate group instead of a phosphate group. The liposomes bind to enzymes, such as a phospholipase, and such liposomes containing a detectable marker may be used as a substrate in an enzyme immunoassay.
4888289 REAGENT FOR DETERMINING CREATINE KINASE Tetuji Takami, Hiroyk Tsubota, Hisashi Ochi, Chiba, Japan assigned to Iatron Laboratories Inc; Unitaka L A reagent for the determination of creatine kinase is comprised of a reagent group containing N-acetylcysteine and ethylenediaminetetraacetic acid and a reagent group containing magnesium, whereby the stability thereof is acheived. 4889797 DRY ANALYTICAL ELEMENT HAVING A SPREAD CONTROL AREA FOR ASSAYING ENZYME ACTIVITY Yoshikazu Amano, Shigeki Kageyama, Harumi Katsuyama, Saitama, Japan assigned to Fuji Photo Film Co Ltd A dry analytical element for assaying an enzyme in a liquid is disclosed, comprising a support having provided thereon at least a porous liquidspreading layer composed of water nonabsorbent fibers, wherein said porous liquidspreading layer contains a substrate for an enzyme to be assayed and a hydrophilic polymer in an amount effective to decrease a spreading
4889818 PURIFIED
THERMOSTABLE ENZYME
David H Gelfand, Susanne Stoffel, Frances C Lawyer, Randall K Saiki assigned to Cetus Corporation A purified thermostable enzyme is obtained that has unique characteristics. Preferably the enzyme is isolated from the Thermus aquaticus species and has a molecular weight of about 86,000-90,000 daltons. The thermostable enzyme may be native or recombinant and may be used in a temperature-cycling chain reaction wherein at least one nucleic acid sequence is amplified in quantity from an existing sequence with the aid of selected primers and nucleotide triphosphates. The enzyme is preferably stored in a buffer of non-ionic detergents that lends stability to the enzyme.
4891104 ENZYMATIC ELECTRODE AND ELECTRODE MODULE AND METHOD OF USE Max D Liston, Paul K Hsei, Christopher C Feistel assigned to SmithKline Diagnostics Inc An improved enzymatic electrode and electrode module specifically adapted for use in a medical analyzer device is disclosed which permits rapid analysis of substances of interest contained within undiluted body fluids such as whole blood, serum and/or plasma. The enzymatic electrode is in fluid communication with an axially reciprocating probe which selectively transports either a quantity of a buffer aqueous solution or a calibrant aqueous solution presented at a wash cell or a body fluid specimen disposed within a sample cup to an active enzyme bearing membrane positioned adjacent the enzymatic electrode. The membrane comprises a composite membrane structure having a protective membrane layer adapted to prevent the passage of blood cells and other particulate or cellular substances therethrough as well as adjust the diffusion rate of the substance of interest desired to be measured into the membrane, an active enzyme layer adapted to convert the
PATENT ABSTRACTS glass, aminated zeolite, or water insoluble crosslinked chitosen,
area by at least 20~o. The analytical element exhibits high sensitivity and high reproducibility.
4888288 VESICLES RESISTANT TO ENZYME LYSIS AND USE THEREOF IN AN ENZYME ASSAY Daniel Wagner assigned to Becton Dickinson and Company Liposomes are formed from compounds ineluding a phosphonate or phosphinate group instead of a phosphate group. The liposomes bind to enzymes, such as a phospholipase, and such liposomes containing a detectable marker may be used as a substrate in an enzyme immunoassay.
4888289 REAGENT FOR DETERMINING CREATINE KINASE Tetuji Takami, Hiroyk Tsubota, Hisashi Ochi, Chiba, Japan assigned to Iatron Laboratories Inc; Unitaka L A reagent for the determination of creatine kinase is comprised of a reagent group containing N-acetylcysteine and ethylenediaminetetraacetic acid and a reagent group containing magnesium, whereby the stability thereof is acheived. 4889797 DRY ANALYTICAL ELEMENT HAVING A SPREAD CONTROL AREA FOR ASSAYING ENZYME ACTIVITY Yoshikazu Amano, Shigeki Kageyama, Harumi Katsuyama, Saitama, Japan assigned to Fuji Photo Film Co Ltd A dry analytical element for assaying an enzyme in a liquid is disclosed, comprising a support having provided thereon at least a porous liquidspreading layer composed of water nonabsorbent fibers, wherein said porous liquidspreading layer contains a substrate for an enzyme to be assayed and a hydrophilic polymer in an amount effective to decrease a spreading
4889818 PURIFIED
THERMOSTABLE ENZYME
David H Gelfand, Susanne Stoffel, Frances C Lawyer, Randall K Saiki assigned to Cetus Corporation A purified thermostable enzyme is obtained that has unique characteristics. Preferably the enzyme is isolated from the Thermus aquaticus species and has a molecular weight of about 86,000-90,000 daltons. The thermostable enzyme may be native or recombinant and may be used in a temperature-cycling chain reaction wherein at least one nucleic acid sequence is amplified in quantity from an existing sequence with the aid of selected primers and nucleotide triphosphates. The enzyme is preferably stored in a buffer of non-ionic detergents that lends stability to the enzyme.
4891104 ENZYMATIC ELECTRODE AND ELECTRODE MODULE AND METHOD OF USE Max D Liston, Paul K Hsei, Christopher C Feistel assigned to SmithKline Diagnostics Inc An improved enzymatic electrode and electrode module specifically adapted for use in a medical analyzer device is disclosed which permits rapid analysis of substances of interest contained within undiluted body fluids such as whole blood, serum and/or plasma. The enzymatic electrode is in fluid communication with an axially reciprocating probe which selectively transports either a quantity of a buffer aqueous solution or a calibrant aqueous solution presented at a wash cell or a body fluid specimen disposed within a sample cup to an active enzyme bearing membrane positioned adjacent the enzymatic electrode. The membrane comprises a composite membrane structure having a protective membrane layer adapted to prevent the passage of blood cells and other particulate or cellular substances therethrough as well as adjust the diffusion rate of the substance of interest desired to be measured into the membrane, an active enzyme layer adapted to convert the