5595877 Methods of producing nucleic acid ligands

504

PATENT ABSTRACTS

and/or unlike species are present.

5595877 METHODS NUCLEIC

OF PRODUCING ACID LIGANDS

Gold Larry; Tuerk Craig Boulder, CO, UNITED STATES Assigned to NeXstar Pharmaceuticals lnc The present invention includes methods for the identification and production of improved nucleic acid ligands based on the SELEX process. Also included are nucleic acid ligands to the HIV-RT protein identified according to the methods described therein.

5595878 DETECTION

OF

BIOPOLYMERS AND BIOOLIGOMERS WITH BORON HYDRIDE

LABELS

Sood Anup; Spielvogel Bernard F Durham, NC, UNITED STATES Assigned to Boron Biologicals Inc A method is disclosed for detecting boron labeled biopolymers, biooligomers, and other species that bind to the biopolymers, and biooligomers with specificity. The biopolymers include RNA, DNA and proteins. The biooligomers include RNA oligomers, DNA oligomers, and peptides. The biopolymers and biooligomers are labeled with a borane, or a boron-hydride moiety. The borane and boron-hydride moiety are employed to reduce a metal ion, e.g. silver, to metal or metal oxide, metal boride or other metal species, or alternatively to reduce a dye, or another organic or inorganic compound. The product of the reduction reaction can be visualized with or without signal augmentation, by one or more means, including the naked eye or a microscope, or can be detected through the use of analytical equipment that can detect conductivity, voltage, density, ultraviolet or visible light, fluorescence, luminescence, phosphorescence, paramagnetism, magnetic susceptibility, or any other detectable physical property. The invention provides methods for selectively detecting biopolymers and biooligomers that contain such a boron-label, thereby permitting their selective identification within an environment where unlabeled like

5595879 MULTI-DOMAIN DNA LIGANDS FOR PROTEIN AND NUCLEIC ACID AFFINITY CHROMATOGRAPHY AND PROCESSING OF SOLID-PHASE DNA Utermohlen Joseph G Tucson, AZ, UNITED STATES Assigned to Arizona Board of Regents for and on behalf of the University of Arizona The present invention is directed to a multi-domain oligonucleotide ligand having multiple functions conferred by each of the domains. The ligand is bound to a solid phase matrix for use in both mRNA-affinity chromatography and as a priming matrix for generating matrix-bound cDNA from the mRNA bound to the matrix. The resultant cDNA was tested and found to function as a solid-phase template for PCR. This solid-phase template approach to PCR is demonstrated with oligo-d(T) paper. The functional domains are defined by polynucleotide sequences on the ligand to confer the different functions.

5595880 DNA ENCODING ADRENERGIC

AN ALPHA2B

RECEPTOR

AND

USES THEREOF Weinshank Richard L; Hartig Paul R New York, NY, UNITED STATES Assigned to Synaptic Pharmaceutical Corporation The present invention provides an isolated nucleic acid molecule encoding an alpha2B-adrenergic receptor, and an isolated protein which is a human alpha2B-adrenergic receptor. The invention also provides vectors comprising DNA molecules encoding a human alpha2B-adrenergic receptor, and vectors adapted for expression of the alpha2b2B-adranexgic receptor in bacterial, yeast, or mammalian cells. In addition, the invention provides a DNA probe useful for detecting nucleic acid encoding the alpha2b2B-adrenergic