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List of contents, subject index and author index volume 3, 1972
JOURNAL
OF STEROID
AND MOLECULAR ~
--In__..
BIOCHEMISTRY
BIOLOGY,
._..
THE _
editors-in-chief
J. R. PASQUALINI and R. SCHOLLER
Volume 3 1972 ‘ACC@tDING TO: PROJECT MANAGEM~T E-MAIL DATE: JULY 29,2002 SUBJECT: DPS TITLES OLD TITLE: THE JOURNAL OF STEROID BIOCHEMISTRY NEW TITLE: JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, THE OLD ISSN: NEW ISSN: REMARKS: ARCHIVING DEPARTMENT
*
@
Pergamon
Pl’eSS
Oxford
New York
Brumrchweig
J. R. PASQUALINI and R. SCHOLLER, Foundation for Hormone Research, 26 Boulevard Brune, Paris 14e, France
ASSOCIATE H. H. A. A.
EDITORS
ADLERCREUTZ, Helsinki, Finland H. BREUER,Bonn, Germany J. K. GRANT, Glasgow, Scotland CARSTENSEN, Umea, Sweden H. J. VANDERMOLEN,Rotterdam, Netherlands E, KELLIE, London, England J. K. NORYMBERSKI, Sheffield, England MUNCK, Hanover, N.H., U.S.A. G. W. OERTEL,Mainz, Germany
CORRESPONDING
EDITORS
A. AAKVAAG,Oslo, Norway * A. E. ARG~~ELLES, Buenos Aires, Argentina - M. K. BIRMINGHAM, Montreal, Canada - A. 0. BOSCH,Madrid, Spain * J. P. COGHLAN, Victoria, Australia * C. CONTI,Rome, Italy * E. COUTINHO,Bahia, Brazil + J. CRABBY?, Leuven, BeIgium * 0. DOMINGUEZ,Mexico City, Mexico - K. B. EIK-NES, Los Angeles, Calif., U.S.A. * A. ERCOLI,Milan, Italy - V. A. FRANDSEN,Copenhagen, Denmark - E. GURPIDE,Minneapolis, U.S.A. * R. IGLESIAS,Santiago, Chile - E. V. JENSEN, Chicago, Ill., U.S.A. - M. G. KOLPAKOV,Novosibirsk, U.S.S.R. . S. KORJZNMAN, Iowa. U.S.A. . C. P. LANTOS,Buenos Aires, Argentina - L. MARTINI, Milan, Italy - K. MC~RNS, Gainesville, Fforida, U.S.A. - P. DE MOOR, Leuven, Zurich, Switzerland - M. SA~RAN, Toledo, Ohio, U.S.A. Belgium + J. MUELLER, B. W. O’MALLEY,Nashville, Tennessee, U.S.A. * T. SANDOR,Montreal, Canada * A. SPAT, K. SCHUBERT,Jena, G. D. R. - L. A. SOBREVILLA,Lima, Peru - K. F. ST~A, Bergen, Budapest, Hungary + L. STARKA,Prague, Czechoslovakia Ghent, Belgium - R. Norway - B. TAMAOKI,Chiba-shi, Japan * A. VERMEULEN, VIHKO, Helsinki, Finland
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coNTlxNTs
Number 1
Editors’ Addresses . . . . . . . . . . . Steroid Nomenclature . . . . . . . . . . . Announcement . . . . . . . . . . . . G. S. RAO, MARIE L. RAO and H. BREUER: Studies on a solubilized oestriol 16a-glucuronyltransferase from human liver microsomes . , . . . A. M. PORTO, F. E. BARALLE and E. G. GROS: Biosynthesis of bufadienolides in toads-III. Experiments with [2-14C]mevaIonic acid, [20-14C]3~-hydroxy-5pregnen-20-one and [20-‘*C]cholesterol . . . . . . . A. DESSYPRIS and H. ADLERCREUTZ: Routine clinical determination of testosterone in plasma by competitive-protein-binding . . . . . J. AHMED and A. E. KELLIE: The excretion of oestrogen conjugates in late pregnancy urine . . . . . . . . . . . L. STARKA, R. HAMPL and J. OBENBERGER: Corticosterone in the aqueous humour of the rabbit eye . . . . . . . . . . Short Communication H. WATANABE, P. TOFT and J. A. MENZIES: Conjugation of 2-hydroxyoestrone in rat bife . . . . . . . . . . . General Review D. B. GOWER: 16unsaturated Cl9 steroids; a review of their chemistry, biochemistry and possible physiological role . . . . . . .
i . ..
111 ... VI11
1
11 I9 31 39
43
45
Nun&r 2 Symposium on Aldosterone and Active Sodium Transport Across Epithelia Basel, 23-24 July 1971 Introduction . . . . . Opening remarks: Professors H. TH~LEN, A. WETTSTEIN . . . . Physiological and Morphological
.
.
.
.
.
F. ROLLER, T. ~IC~STEIN .
,
.
.
.
.
V
and .
.
105
Aspects of Aldosterone Action
E. HVXID LARSEN: Characteristics of aldosterone stimulated transport in isolated skin of the toad, Bufo bafo (L-1 . . Discussion by: CrabbB, Hviid Larsen, Morel, Ussing and VoQte . R. NIELSEN: The effect of polyene antibiotics on the aldosterone induced changes in sodium transport across isolated frog skin . Discussion by : Edelman, Leaf, Nielsen, Nutbourne, Ussing and Wiederholt ... 111
111
121
R. S. SNART: The two stage nature of the aldosterone response Discussion by: Edelman, Fanestil, Handler, Snart and Wiederholt J. S. HANDLER, sodium content bladder Discussion by: and Snart
.
.
.
129
A. S. PRESTON and J. ORLOFF: Effect of aldosterone on the and energy metabolism of epithelial cells of the toad urinary Crabbe, De Weer, Edelman, Fanestii, Handler, Jorgensen, Leaf _ . . . . . . . . . . .
C. J. EDMONDS: Effect of aldosterone on mammalian intestine Discussion by: Crabbe, Edelman, Edmonds, Handler, Leaf and Porter
137
.
143
M. WIEDERHOLT, C. BEHN, W. SCHOO~ANS and L. HANSEN: EfIect of aldosterone on sodium and potassium transport in the kidney . . . . Discussion by: Edelman, Fanestil Snart and Wiederholt.
151
C. L. VOUTE, S. HANNI and E. AMMANN: Aldosterone induced mo~hologica~ changes in amphibian epithelia in viva . _ _ Discussion by: Crabbe, Edelman, Leaf, Porter and Vofite .
161
I
Biochemical Asvects of Aldosterone Action I. S. EDELMAN: The initiation mechanism in the action of aldosterone on sodium transport Discussion by: Edelman, Handler and Pas~ualiui . . . . . .
167
E. KIRSTEN, R. KIRSTEN and A. SALIBIAN: A study on the effizct of aldosterone on the extramitochondrial adenine nucleotide system in rat kidney . . Discussion by : CrabbB, De Weer, Edelman, Kirsten, Leaf and Snart .
173
P. L. JMRGENSEN: The role of aldosterone in the regulation of (Na* + KS)ATPase in rat kidney . . . Discussion by: De Weer, Jsrgensen, Kirsten and Wiederholt
181
J. H. LUDENS, J, R. DEVRIES and D. D. FANESTIL: chromatography of aldostreon~binding macromolecules Discussion by: FanestiI, Funder and Snart . . .
.
193
G. A. PORTER and J. KIMSEY: The effect of a new anti-a~dosterone agent SC19886 on aldosterone-stimulated transepithelial sodium transport Discussion by: Edelman, Eigler and Porter . . . . . . _
201
J. W. FUNDER, D. FELDMAN and I. S. EDELMAN: Specific aldosterone binding in rat kidney and parotid . . . . Discussion by: Alberti, Funder, Handler and Rousseau
209
Studies on affinity .
.
.
G. ROUSSEAU, J. D. BAXTER, J. W. FUNDER, TOMKINS : G~ucoco~icoid and miner~oco~~oid
I. S. EDELMAN and G. M. receptors for aldosterone . . . . Discussion by: Funder, Jorgensen, Leaf and Rousseau .
219
J. CRABBE: Hormonal influences on transepithehal sodium transport: aldosterone vs. insulin _ . . Discussion by: Crabbe, Edelman, Hviid Larsen and Ussing .
229
A. LEAF and A. D. C. MACKNIGHT: The site of the aldosterone induced stimulation of sodium transport Discussion by: Alberti, Cereijido, Crabbe, Edelman, Edmonds, Leaf and Porter
237
A. LEAF: Concluding remarks
.
.
.
iv
.
.
.
.
.
,
247
Number 3
First International
Symposium of the Journal of Steroid Biochemistry
THE INTERACTION OF STEROIDS WITH MACROMOLECULES Opening remarks by J. R. PASQUALINI Radioimmunassay
.
.
.
.
.
.
.
251
and competitive binding assay of steroids
D. RODBARD and K. J. CATT: Mathematical theory of radioligand assays: the kinetics of separation of bound from free Discussion by: Exley, Kellie, Rodbard and Vermeulen . . . . . A. E. KELLIE, V. K. SAMUEL, W. J. RILEY and D. M. ROBERTSON: Steroid glucuronoside-BSA complexes as antigens : the radioimmunoassay of steroid conjugates Discussion by: Adlercreutz, Grant, Kellie, Munck and Siiteri . . . . R. P. EKINS, G. B. NEWMAN, R. PIYASENA, P. BANKS and J. D. H. SLATER: The radioimmunoassay of aldosterone in serum and urine : theoretical and practical aspects Discussion by: Ekins, Pasqualini, Rodbard and Slater . . . . . M. CASTANIER, J. GRENIER and R. SCHOLLER: A direct radioimmunoassay for an estrone and estradiol-l7fl fraction in human urine Discussion by: De Moor and Grenier . . . . . . . H. VAN BAELEN and P. DE MOOR: Steroid-protein interaction as studied by isoelectric focusing Discussion by: Rodbard, Rosner and Van Baelen . . . . . . TN. J. BENRAAD, F. H. DE JONG, H. J. VAN DER MOLEN and A. G. H. SMALS : Comparison and evaluation of a competitive protein binding and a gaschromatographic method for the assay of testosterone in peripheral human plasma Discussion by: CrabbC, Munck and van der Molen . . . . . W. P. COLLINS, M. D. MANSFIELD, N. S. ALLADINA and I. F. SOMMERVILLE : Radioimmunoassay of plasma testosterone Discussion by: Collins, Exley, Fazekas (A. G.), Munck and Rosner . . M. A. PIZARRO and J. KOLANOWSKI: Problems arising from blanks in protein-binding analysis Discussion by: Exley, Haukkamaa, Munck, Piyasena and Pizarro . . W. J. RILEY, E. R. SMITH, D. M. ROBERTSON and A. E. KELLIE: The formation of steroid-BSA antigens via a C-6p-carboxymethyl substituent Discussion by : Exley, Munck and Smith . . . . . . General discussion by: Collins, Cooke, De Moor, Ekins, Exley, Fazekas (A.T.A.; Grant, Kellie, Munck, Rodbard and van der Molen . . . . .
255
275
289
305
321
325
333
349
357 369
Receptors and mechanism of action of androgens-I A. AAKVAAG, K. J. TVETER, 0. UNHJEM and A. ATTRAMADAL: Receptors and binding of androgens in the prostrate Discussion by: Aakvaag, Exley, Grant, Kellie, Liao, Morfin, Pasqualini, Siiteri, Tuohimaa and Vermeulen . . . . . . . . . . V
375
R. MASSA, E. STUPNICKA, Z. KNIEWALD and L. MARTINI : The transformation of testosterone into dihydrotestosterone by the brain and the anterior pituitary Discussion by: CrabbC, De Moor, Exley, Fazekas (A. G.), Fazekas (A. T. A.), Grant, Martini, Munck, O’Malley, Siiteri and van der Molen . . . . S. LIAO, T. LIANG and J. L. TYMOCZKO: Structural recognitions in the interactions of androgens and receptor proteins and in their association with nuclear acceptor components Discussion by: Jensen, Kellie, Liao, Munck and Wira . . . . . 0. A. LEA and K. F. ST0A: The binding of testosterone to different serum proteins : a comparative study Discussion by: Lea, Munck and Rosner . . . . . . . A. VERMEULEN and L. VERDONCK: Some studies on the biological significance of free testosterone Discussion by: CrabbC, De Moor, Martini, Munck, Slater and Vermeulen . V. HANSSON, K. J. TVETER, 0. UNHJEM and 0. DJOSELAND: Studies on the interaction between androgen and macromolecules in male accessory sex organs of rat and man Discussion by: Hansson and Liao . . . . . . . . General discussion by: Aakvaag, Concolino, CrabbC, De Moor, Liao, Martini, Massa, Morfin, Munck, Pasqualini and Tuohimaa . . . . .
Receptors
and mechanism
of action
401
409
421
421 441
of oestrogens-II
E. V. JENSEN, S. MOHLA, T. GORELL, S. TANAKA and E. R. DESOMBRE Estrophile to nucleophile in two easy steps Discussion by: Adlercreutz, Jensen, Munck, Rosner and Wira. . . . P. K. SIITERI, R. ASHBY, B. SCHWARZ and P. C. MACDONALD : Mechanism of estrogen action studies in the human Discussion by: CrabbC, Exley, Grant, Jensen, Munck, O’Malley, Siiteri, Sommerville, Tuohimaa and Wira . , . . . . . . . . J. K. GRANT and ELEONORA P. GIORGI: A dynamic study of androgen binding to macromolecules in the human prostrate Discussion by: Collins, Grant, Jensen, Morfin and Munck . . . . A. G. FAZEKAS and T. SANDOR: Metabolism of androgens by isolated human hair follicles Discussion by: Fazekas (A. G.), Liao, Marlin and O’Malley . . . . G. W. OERTEL and P. BENES: The effects of steroids on glucose-6-phosphate dehydrogenase Discussion by: Fazekas (A. G.), Liao and Oertel . . . . . . D. EXLEY: Specificities of antibodies to oestrogens Discussion by: Adlercruetz, Exley, Grant and Kellie . . . . . P. J. TUOHIMAA, S. J. SEGAL and S. S. KOIDE: Study on uterine ribonucleic acid with estrogenic activity Discussion by: Liao, Munck, O’Malley, Siiteri and Tuohimaa . . . D. TOFT: The interaction of uterine estrogen receptors with DNA . . . General discussion by: Adlercreutz, Exley, Grant, Kellie, Jensen, Liao, Morfin, Munck, O’Malley, Rodbard and Siiteri . . . . . . . vi
385
445
459
471
485
493 497
503 515 523
Receptors and mechanism of action of corticosteroids W. ROSNER: Recent studies on the binding of cortisol in serum Discussion by: Crabbe, De Moor, Holzbauer, Munck, Rosner, Siiteri, Slater and Vermeulen . . . . . . . . . . . .
531
J. R. PASQUALINI, C. SUMIDA and C. GELLY: Mineralocorticosteroid receptors in the foetal compartment Discussion by: Crabbe, Fazekas (A. G.), Martini, Munck, O’Malley, Pasqualini, Slater and Spat . . . . . . . . . . .
543
J. CRABBE: Aldosterone: mechanism of action on isolated sodium-transporting epithelia Discussion by: Adlercreutz, Crabbe and Kellie . . . . . .
557
A. MUNCK, C. WIRA, D. A. YOUNG, K. M. MOSHER, C. HALLAHAN and P. A. BELL: Glucocorticoid-receptor complexes and the earliest steps in the action of glucocorticoids on thymus cells Discussion by: Jensen, Martini, Munck, O’Malley, Pasqualini and Rosner .
567
MARGARETHE
HOLZBAUER:
The association of steroids with blood cells in
vivo
Discussion by: Brinkmann, Carstensen, Holzbauer, Kellie, Martini and Slater .
579
P. DE MOOR, W. HEYNS and R. BOUILLON: Growth hormone and the steroid binding j3-globulin of human plasma Discussion by: De Moor, Martini, Morfin and Vermeulen . . . .
593
Receptors and mechanism of action of progesterone A. 0. BRINKMAN, E. MULDER and H. J. VAN DER MOLEN: Model studies with erythrocytes on the initial steps of cellular uptake and binding of steroids Discussion by: Brinkmann, Holzbauer, Jensen, Oertel, van der Molen and Wira
601
B. W. O’MALLEY and W. T. SCHRADER: Progesterone receptor components: identification of subunits binding to the target-cell genome Discussion by: Crabbe, Jensen, Munck, O’Malley, Siiteri, van der Molen and Wira . . . . . . . . . . . . .
617
M. HAUKKAMAA, 0. KARJALAINEN and T. LUUKKAINEN: In vitro binding of progesterone by the human endometrium during the mestrual cycle . . . and by hyperplastic, atrophic and carcinomatous endometrium
631
M. HAUKKAMAA, K. WICHMANN and T. LUUKKAINEN: Effect of phospholipids on the subcellular distribution of progesterone in pregnant rat myometrium and on protein binding of progesterone Discussion by: Haukkamaa, Jensen, Rosner, Siiteri, Tuohimaa and Wichmann .
633
D. A. STANSFIELD, D. J. FRANKS, G. H. WILKINSON and JANET R. HORNE: Studies in the formation and degradation of adenosine 3’S_cylic monophosphate in corpus luteum Discussion by : Adlercreutz, Cooke, Crabbe, Liao, Munck, Siiteri, Spat, Stansfield and van der Molen . . . . . . . . . . .
643
General discussion by: Crabbe, De Moor, Grant, Kellie, Liao, Martini, Massa, . Morfln, Munck, O’Malley, Pasqualini, Siiteri, van der Molen and Wira .
655
Vii
Number 4 T. SANDOR, A. G. FAZEKAS, J.-G. LEHOUX, HELENE LEBLANC and A. LANTHIER: Studies on the biosynthesis of I&oxygenated steroids from exogenous corticosterone by domestic duck (Anus pZ~#yrhy~cho~) adrenal gland mitochondria . . . . . . . . . . . . H. LEBLANC, J.-G. LEHOUX et T. SANDOR: Etude de la 21-hydroxyiase des . . . glandes surrenale 4 du canard domsetique (Anas platyrhynchos) . G. TELEGDY, M. ROBIN and E. DICZFALUSY: A study of sterol and steroid . synthesis from sodium acetate by human foetal liver preparations W. G. STILLWELL, E. C. HORNING, M. G. HORNING, R. N. STILLWELL and A. ZLATKIS: Characterization of metabolites of steroid contraceptives by gas chromatography and mass spectrometry . . . . . . M. E. GIEBENHAIN, G. E. TAGATZ and E. GURPIDE: Serum levels of unconjugated estetrol-(1,3,5(1 O)-estratrien-3, I%, 16a,I7&tetrol) during human pregnancy . . , . . . . . . . . . T. L~TIK~NEN, J. PERHEENTUPA, R. VIHKO, I. MAKINO and J. SJijVALL: Bile acids and hormonal steroids in biie of a boy with 3/3-hydroxysteroid dehydrogenase deficency . . . . . . . . . M. LUISI and C. FASSORRA: The effect of mesterolone upon the concentration of testosterone in rat testis. . . . . , . . . . . G. CONCOLINO and A. MAROCCHI: A simple procedure for the combined determination of plasma estrogen and androgen concentrations by competitive protein binding analysis . . . . . . . . . . J. R. MUELLER, J. A. ROBINETTE and M. E. YANNONE: An evaluation of “OV” liquid phases for the gas-liquid ~hromato~aphic separation of biologically active steroids . . . . . . . . _ . . . E. M. CHAMBAZ, C. MADANI and A. ROS: TMS-enol-TMS: a new type of derivative for the gas phase study of dihydroxyacetone side chain saturated corticosteroid metabolites . . . . . . . . . . J.-P. WENIGER et A. ZEIS : Mttabolisme de la [4-‘4C]-testostCrone par le testicule embryonnaire de rat cultive in vitro . . . . . . . . Short Communication A. SPAT and SARA J6ZAN: Competitive protein binding assay of corticosterone Errata . . . . . . . . . . . . . Announcements . . . . . . . . . . . .
661 683 693
699
707
715 721
725
735
741 749 755 761 765
Number 5 MARGARETHE HOLZBAUER, MARION K. BIRMINGHAM, A. DE NICOLA, UWA GODDEN and HELL1 TRAIKOV: Steroid secretion by rat . . adrenal glands during the first week following adrenal enucleation . J.-G. LEHOUX, A. G. FAZEKAS, H. LEBLANC, A. CHAPDELAINE and T. SANDOR: Pteridins and steroid hydroxylation. I-The effect of folic acid and aminopterin on the steroid 11/3- and 21-hydroxylation by domestic duck (Anas platyrhymhos) adrenocortical tissue preparations . . . . . , S. SULIMOVICI and B. LUNENFELD: The effect of adenosine 3’,5’-cyclicmonophosphoric acid on the 17~-hydroxysteroid dehydrogenase of rat testis v 1.. Vlll
767
773 781
R. I. SALGANIK, N. P. MERTVETSOV and S. V. ARGUTINSKAYA: The patterns of RNA synthesis and induction of glycogenic enzymes in rat liver under continuous cortisol administration . . . . . . . . P. BENES and G. W. OERTEL: Inhibition of glucosed-phosphate dehydrogenase by steroids. IV-Effects of C,,-steroids upon human placental glucose-6 phosphate dehydrogenase . . . . . . . . . . J. MINGUELL and J. K. GRANT: Rat bone marrow cells: androgen metabolism and actions at the molecular level . . . . . . . . . M. J. TIKKANEN and H. ADLERCREUTZ: A method for the quantitative determination of urinary estriol conjugates in human pregnancy . . . F. BERTHOU, L. BARDOU and H. H. FLOCH: Identification of Sa-androstane3,9,17&diol in human urine . . . . . . . . . F. GALLETTI and R. GARDI: Metabolism of 1-dehydroandrostanes in man. IIMetabolism of 5fl-androst-1-enes. . . . . . . . . A. F. CLARK, R. S. CALANDRA and C. E. BIRD: Kinetics of [3H]-testosterone acetate metabolism in normal young men: effects of medroxyprogesterone (provera) administration . . . . . . . . . . I. BELIC and H. SONIC: Microbial dehydrogenation of tomatidine . . .
791
799
803 807 819 831
837 843
Number 6 A. HAKSAR, F. G. PERON and W. F. ROBIDOUX, JR: The possible function of pyruvate in the steroidogenic response of rat adrenal cell suspensions to ACTH, cyclic-3’,5’-AMP and dibutyryl cyclic-3’,5’-AMP . . . . . . A. M. BONGIOVANNI and J. MARINO: Steroid dehydrogenases in rabbit liver of various developmental stages . . . . . . . . . A. Z. MEHDI and T. SANDOR : Product-precursor relationship during incubation of duck adrenal slices . . . . . . . . . . V. LAUMAS, A. FAROOQ and K. R. LAUMAS : Priming effect of norethynodrel on the uptake of (6,7-3H)-estradiol in the mouse uterus . P. ENEROTH, J.-A. GUSTAFSSON, H. FERNGREN and B: HELLSTROM : Excretion and anticonvulsant activity of steroid hormones in an infant with infantile spasm and hypsarrhytmia treated with excessive doses of ACTH . . G. FALCONI, G. L. ROSSI, A. ERCOLI and R. GARDI: Cyclopentyl ethers of estrogenic steroids. Parallelism between prolonged oral activity and storage in . . . . . . body fat of rats E. YOUSSEFNEJADGN, E. FLORENSA, W. P. COLLINS and I. F. SOMMER: VILLE: Radioimmunoassay of plasma progesterone . . . . . G. W. OERTEL and P. BENES: Inhibition of glucose&phosphate dehydrogenase by steroids. VI-Effects of new synthetic dehydroepiandrosterone conjugates upon the activity of human placental glucosed-phosphate dehydrogenase . . M. INABA, K. KAMATA, S. IMAI and T. NAKAO: Studies on secretion mechanism of adrenal corticosterone: adrenal subcellular distribution of corticosterone and its changes on hypophysectomy and ACTH administration . E. V. YOUNGLAI and C. PELLETIER: A competitive protein-binding method for the measurement of progesterone in human amniotic fluid . . . . P. BENES and G. W. OERTEL: On steroid conjugates in plasma. XXIII-Biosynthesis of steroid sulfatide from a cytidine phosphosulfate steroid complex . ix
847 859 865 871
877
889 893
903
907 919 925
F. GALLETTI and R. GARDI: Metabolism of I-dehydroandrostanes in man. III-Metabolism of 17/3-hydroxy-Sa-androst-1-en-3-one, 17/3-(l’-methoxycyclohexyloxy)-5a-androst-I-en-3-one (mesabolone) and Sa-androst-1-ene-3,17-dione R. E. SHEPHERD and R. S. SNART: Oestradiol induced respiratory and enzymic changes in rat uterus . . . . . . . . . . Short Communications A. DESSYPRIS, A. REPONEN, S.-L. KARONEN and H. ADLERCREUTZ: . Technical improvements to a method for determination of testosterone . J. CHAMBERLAIN and A. W. ROGERS : The appearance of dihydrotestosterone3H in the diencephalon of neonatal rats after subcutaneous injection of testosterone-3H . . . . . . . . . . . .
933 937
943
945
ACTH (adrenocorticotrophin) effect on corticosterone secretion in hypophysectomized rats, 907 effect on steroid hormone excretion, on an infant with h~sa~hytmia and infantiie spasm, 877 effect on steroidogenesis, in rat adrenal cell suspensions, 847 Adenine nucleotide effect of aldosterone on, in extramitochondrial fraction of rat kidney, 173 Adenosine 3’,5’-cyclic monophosphate effect on 17jLhydroxysteroid dehydrogenase, in rat testis, 781 formation and degradation of, in rat corpus luteum, 643 Adrenal(s) biosynthesis of l&oxygenated steroids, in duck, 661 effect of folic acid and aminopterin on steroid ll/% and 21-hydroxylations by duck, 773 effect of pyruvate in steroidogenesis of rat, 847 steroid secretions in rat, following enucleation, 767 subcellular distribution of corticosterone in hypophysectomized rat, 907 transformation of [4-‘4C]progesterone in duck, 865 Afhnity chromatography isolation in human serum of CBG (corticosteroid binding globulin) by, 53 1 studies on aldosterone-binding macromolecules, 193 Aldosterone and morphological changes in amphibian epithelia in vim, 161 and polyene antibiotics, effect of, on sodium transport in frog skin, 121 and two specific binding sites in isolated toad bladder, 129 binding macromo~~ules and studies by affinity chromatography, 193 biosynthesis by adrenais of ducks, 661 effect of a new anti-aldosterone agent: SC 19886 on sodium transport, 201 effect of, in adenine nucleotide system in rat kidney, 173 effect of, on sodium and potassium transport in the rat kidney, 151 effect of, on sodium transport in isolated skin of toad, 111 effect of, on sodium content in epithelial cells of the toad bladder, 137 effect of, on sodium content on mammalian intestine, 143 formation of receptors and mechanism of action in the kidney of adrenalectomized rats, 167 glucocorticoid and mineralocorticoid receptors for, 219 mechanism of action of, on target cells in isolated epitheha, 557 radioimmunoassay of, in serum and urine, 289 receptors, formation in the foetal kidney of guinea pig, 543 role of, in the regulation of (Na+ + K+)-ATPase in the rat kidney, 181 site of action and stimulation on sodium transport, 237 specific bindings in rat kidney and parotid, 209 vs. insulin influences on transepithelial sodium transport, 229 xi
Amniotic fluid measurement
of progesterone by competitive protein-binding method in humans, 919 Androgens binding to macromolecules in human prostate, 471 determination of plasma by competitive protein binding, 725 macromolecule interaction in male accessory sex organs of rat and man, 427 metabolism and actions at the molecular level of, in rat bone marrow cells, 803 metabolism of, by isolated human hair follicles, 485 receptors and binding of, in the prostate, 375 receptor proteins and nuclear acceptors, 401 5fi-Androst-1-enes metabolism in humans, 831 Sa-Androst-1-ene-3,17-dione metabolisms in humans, 933 Sa-Androstane-3j3,17@diol identification of, in human urine, 819 Androstene-3,17-dione metabolism in human male hair follicles, 485 Antibodies specificities to estrogens, 497 Anti-aldosterone effect of SC 19889 compound (a 12a, 13&etiojervane derivative) on aldosteronestimulated transepithelial sodium transport, 201 Aqueous humour corticosterone identification in, of the rabbit eye, 39
Bile 2-hydroxyoestrone conjugation in, 43 Bile acids in the bile of a boy with 3fl-hydroxysteroid dehydrogenase deficiency, 715 Binding aldosterone, in rat kidney and parotid, 209 determination of plasma estrogens and androgens by protein, method, 725 determination of plasma testosterone by competitive protein, method, 19 of androgens to macromolecules in the human prostate, 375, 471 of cortisol in serum, 531 of progesterone, in chick oviduct, 617 of progesterone, in human endometrium, 631 of progesterone in the myometrium of pregnant rats, 633 of steroids to erythrocytes, 601 of testosterone to different serum proteins, 409 protein, and assay for corticosterone, 755 Blood cells “in vivo” association of steroids with, 579 Bone marrow cells metabolism of androgens at the molecular level, in rats, 803 Brain transformation of testosterone into dihydrotestosterone by, 385 xii
Bufadienolides biosynthesis of, in toads, 11 CIa steroids effect of, on human placental glucose-6-phosphate dehydrogenase, 799 C19 steroids conjugates, in the bile of a boy with 3/3-hydroxysteroid dehydrogenase deficiency, 715 16unsaturated, chemistry, biochemistry and physiological role of, 45 CzI steroids conjugates, in the bile of a boy with 3,Shydroxysteroid dehydrogenase deficiency, 715 Cdj3-carboxymethyl steroids formation of bovine serum albumin antigens, via, 357 Chick oviduct receptors of progesterone, 617 Cholesterol and biosynthesis of bufadienolides, in toads, 11 Competitive protein binding determination of estradiol, estrone, testosterone and androstenedione in human plasma, 725 determination of progesterone by, in human amniotic fluid, 919 determination of testosterone by, in peripheral human plasma, 707 Contraceptives characterization of metabolites by gas chromatography and mass spectrometry, 699 Corticosteroids metabolism determination by gas chromatography, 741 Corticosterone association to blood cells, 579 biosynthesis of 1&oxygenated steroids from, in domestic duck, 661 determination by competitive protein binding in rat plasma, 755 in the aqueous humour of the rabbit eye, 39 studies on mechanism of secretion in rats, 907 transformation into l&oxygenated steroids by the adrenals of domestic duck, 661 Cortisol association to blood cells, 579 binding of, in human serum, 531 binding to transcortin, 321. effect of, on RNA synthesis in rat liver, 791 receptors in thymus cells, 567 Cyclic-3’,5’-AMP and corticosterone biosynthesis, in rat adrenals, 847 Cyclopentyl ethers studies on biological activities of estrogenic steroid derivatives, 899 Dehydroepiandrosterone sulphate metabolism in human male hair follicles, 485 transformation of, by mitochondrial preparation ... xl11
of guinea pig liver, 925
gIu~ose-6-~hospha~e, effect of C, *-steroids upon human placenta, 799 3~-hydroxys~eroid deficiency in a boy, 715 17jLhydroxysteroid, effect of adenosine 3’$‘-cychomonophosphoric acid on, of rat testis, 781 Dehydrogenation of toma~idine by the microorganism: ~ucffrd~~restrictz.t.s, 843 Deoxycorticosterone association to blood cehs, 579 secretion by the rat adrenal glands, 767 5u-~ihydrotes~os~~rone binding in epididymis of adult castrated rats, 427 birding to specific receptors in the mi~rosoma1 or cytosohc fractions of rat ventral prostate, 401 in the diencephalon of neonatal rats after injection of testosterone, 945 transformation of testosterone into, by the brain, 385 Dimethisterone (6a-methyl-l7u-(l-propynyl)-I7~-hydroxy-4-androsten-3-one) metabolism in humans, 699 DNA and progesterone binding in chick oviduct, 617 interaction of uterine estrogen receptors with3 515 Duck biosy~~esis of lo-oxygena~d steroids by the adrena& of, 661 effect of folic acid and am~iopte~n on I I@- and Al-bydroxyIa~ions of&e adrenal glands, 773 transformation of [4-r4Cj-progesterone, by the adrenals of, 865
Endometrium in V&O b~uding of progesterone by the human, 631 ~~idid~is binding to Sa-dihydrotestosterone, in adult castrated rats, 427 Epi-estriol 16~-gIuc~r~~oside urinary excretion in late pregnancy, 31 E~i~eIi~ o&Is ef%ectof dldosterone, on sodium content, in toad urinary bladder, 137 Erythrocytes studies on steroid binding, 601 Estetro1-(1,3,5(10)-estratrien-3,15a,16~,17~-tetra1) in human pregnancy plasma, 707 Estradid dete~natiou by competitive protein binding in human pfasma, 725 effect of, in enzymic changes in rat uterus, 937 effect of ~~re~ynodrel on the uptake of, in mouse uterus, 871 17&gIucuronoside, urinary excretion in late pregnancy, 31 radjoi~~~oassay for, in human urine, 305
and ~~-giucu~ony~~ansferase from liver microsomes, 1 dete~i~at~an of conjugates, in human prqyxmcy urine, 807 3-glucuro~~side, urinary excretion in late pregnancy, 31 lo-gluc~ro~oside, urinary excretion in late pregnancy, 31 3-s~~hate-l6u_gluc~onoside, urinary excretion in late ~r~g~an~~ 31 ~s~ogens and antibody s~c~~ci~es~ 497 and uterine ribonucleic acid in rats, 503 c~njugat~s~ urinary excretion in late pre~a~cy~ 3% determination by competitive protein binding, 72.5 specific receptors and mechanism of formative in the rat uterus, 445 studies of mecb~sm of action, in human e~dome~~~ $59 uterine receptor and interactions with DNA, 515 Estrone 3-g~ucuron~side, excretion in late pregnancy, 31 lfia-hydroxy, 16~-glucura~~side, urinary excretion in late pregnancy, 31 16u~hydroxy-3-sulFha~-l6~-glucuronoside~ urinary excretion in late pregnancy, 31 ra~o~~~u~s~y for, in human urine, 305 Foetal compliment ~neraloc~~costeroid receptors in the, 543 Foetal liver sterol and steroid synthesis from sodium a&ate by+ 693 Fohc acid effect of, on steroid Ilp- and 21-hydroxylations by domestic duck, 773 Gas chromatography characterization of contraceptive metabolites, by, 699 de~~~a~o~ of estrogen conjugates by* in urinary human ~~~cy~ 807 d~r~natio~ of ~e~~~~~erQ~~ by, in per~hera~ human plasma> 325 of corticosteroid metabohtes, 741 separation of biologically active steroids, 735 &Globulin steroid binding, in human plasma, 593 ~~ucoco~coid~s~ receptors in thymus ceh, 567 Glucose-~~hosphate d~ydr~ge~ase effects of CIs steroids, upon human placenta, 799 effects ofnew synthetic dehydroepiandrosterot conjugates upon human placenta, 903 effects of difierent steroids on, 493 G~~c~onos~de*~SA (bovine serum ~b~rn~n~ eomp~ex~ used as antigens for radi~i~unoassay of steroid conjugates, 275 Glycogenic enzymes induction of? by cortisol in rat liver, 791 Growth ~o~~~e effect on the binding of different steroids to &#obuhn, 493 xv
Human liver microsomes studies on I&-glucuronyltransferase, I Hydrocortisone (see Cortisol) 1$~~ydroxy-corticost~rone association to blood cells, 579 biosynthesis by the adrenal of duck, 661 1%Hydroxy-deoxycorticosterone secretion by the rat adrenal glands, 767 2-Hydroxyestrone 2-glucuronoside ~o~~~gat~o~ of, in rat bite, 43 16u-~ydro~y~strone 3-gfucuronoside, urinary excretion in late pregnancy, 31 3/3-Hydroxy-5-pregnen-20-one transformation to bufadienolides in toads, 1I I7/?-Hydroxy-Sa-androst-1-en-3-one metabolism of, in man, 933 21-~ydroxylase studies in the adrenals of ducks, 683 11,G-and 21-Hydroxylation by adrenals of domestic duck, effect of folic acid on, 773 3~-~ydroxysteroid dehydrogen~e deficiency of activity, in a pa~o~o~~~a~ case, 715 ~7~~H~dr~xysteroid dehydrogemtse effect of adenosine 3’5’-~ycli~~monophosphoric acid on, in rat testis, 781 Hyperpfastic e~dometr~~m binding of progesterone in, 631 Insulin vs. aldosterone, influences on transepithelial sodium transport, Isoelectric focusing application to steroid-protein interaction, 321
229
Kidney aldosterone binding in rat, and parotid, 209 effect of aldosterone on sodium and potassium transpo~ in rat, 151 effect of aldosterone on the extr~~to~hond~a~ adenine nucteotide in rat, 173 foe&i guinea pig, and mineraXocorticosteroid receptors, 543 role of aldosterone activity on @a* -I- K?J-ATPase, in rat, 181 Liver and steroid dehydrogenase activities in rabbit, 859 human foetal, bios~~esis of sterols and steroids, 693 oestriol, IGa-glucuronyltransferase in microsomes, in humans, 1 MaIe accessory sex organs
androgens and macromolecule interac~ons, of rat and man, 427 ~amma~ian intestine effect of atdosterone on, 143 xvi
Mass spectrometry
characterization of contraceptive metabolites by, 699 M~~~x~ro~sterone acetate elect of [3H]testosterone rnetab~~~~s in normal young men, 837 Menstrual cycle beading of progesterone in the human endometrium~ during, 631 Mesabolone (I?@-(1,’me~oxy-~yclohe~~loxy~“5~-androst-I-en-3-o~e~ metabolism of, in man, 933 Mesterolo~e effect of, upon ~on~ntmtion of testosterone in rat testis, 721 [2- i %I J-Mevalo~~ acid and biosynthesis of b~fadieoo~des in toads, I I M~ne~~I~co~ticosteroids receptors in the foetal compartment, 543 receptors for aldosterone, 219 Mouse effect of nu~~yn~rel on the uptake of [6,~-3H]-estradiol in the uterus of, 8% Myomet~ium effect of phosphol~ids on the su~ll~lar distribntio~ of progesterone in pregnant rat, 633 Neonatal rats ~ydrotestosteron~~3H] in the diencephalon of, 945 Norethisterone ft?u~thynyl-I7~-hyd~oxy-4-estren-3-one) metabolism in humans, 699 Norethynodrel effect of, on the uptake of [6,~-~H~~estradiol in the mouse uterus, 87X Oestriol see estriol Oestrogen see estrogen Oestradiol see estradiol I$-Uxygenated steroids biosynthesis of, by the Adonis
of domestic duck, 661
effect of, on progesterone subcellular distribution in pregnant rat myometrium, 633 Pituitary transformation of testosterone into dihydr~testosterone in anterior, 385 Placenta effects of CIS steroids, upon glu~ose~6-phosphate dehydroge~ase in humans, 799 elect of new synthetic deh~d~iandrosterone ~o~jngates upon glucose-6phosphate dehydrogen~~ in humans, 903 xvii
Plasma assay of testosterone by gas chromatography and competitive protein binding method in peripheral human, 325 determination of estrogens and androgens by competitive protein binding, 725 determination of testosterone in, by competitive protein binding method, 19 growth hormone and steroid binding /LB-globulinin human, 593 protein binding in peripheral human, 325 testosterone evaluation by radi~immu~oass~y, 333 effect of, on aldosterone and sodirrm transport, in frog skin, f2I effect of aldost~rone on transport in rat kidney, 151 effect of aldosterone, on the content of mammalian intestine, 143 Pregnancy and effect of phospholipids on the subceilular distribution of progesterone in rats, 633 and estetrol-(1,3,5(10)-estratrien-3, I&, 1tia, 17,%tetrol) evaluation in humans, 707 determjnation of urinary estriol conjugates in human, 807 urinary excretion of estrogen conjugates, 31 [20- *4~~-~r~gneno~one and biosynth~~s of bufad~e~o~ides, in toads, If association to bfood eelfs, 579 z&m binding of, in human e~d~metrjnrn during menstrual q&e, 631 measurement of, by competitive protein binding in human amniotic fluid, 919 r~~ioimmunoassay of, in plasma, 893 receptors, in target cell gename, 617 subcellular distribution of, in pregnant rat myometrium, 633 in
Prostate androgen binding to ma~romolec~Ies in the human, 471 receptors of androg~ns in the rat, 375 Proteins and androgen receptors in the prostate, 401 and steroids binding, studied by isoelectric fo~us~~~~ 321 binding to progesterone, in pregnant rat myometrium, 633 binding to corticosteroids and progesterone, and problems arising from blanks, 349 binding to testosterone, 19, 409 R&dins and steroid hydroxylations,
773
Rabbit(s) steroid dehydrogenase a~i~viti~s in the liver of, 859 ~orticosterune in the aqueous humour of, 39 _I. XWU
Radioimmunoassay evaluation of estriol and estetrol in pregnancy urine by, 707 of aldosterone in urine and plasma, 289 of plasma progesterone, 893 of testosterone in plasma, 333 studies between steroid and macromolecules of estrone and estradiol urine, 305 theoretical model and mathematical consideration for the radioligand,
in human 255
Rat(s) bile and conjugation of 2-hydroxyestrone, 43 body fat and effect of estrogenic steroids on, 889 bone marrow cells, androgen metabolism and actions at the molecular level, 803 ~3H]-dihydrotestosterone in the diencephalon of neonatal, 945 effect of mesterolone upon testosterone on, 721 effect of phospholipids in the myometrium of, during pregnancy, 633 interactions between androgens and macromolecules in male sex organs of, 427 kidney and effect of aldosterone in the regulation of the (Na+ + K+), 181 kidney and parotid aldosterone binding in, 209 kidney and effect of ddosterone on adenine nucleotide system in, 173 liver, effect of cortisol in glycogenic enzymes, 791 studies on mechanisms of corticosterone secretion, 907 testis and effect of adenosine 3’5’-cyclic-monophosphoric acid on 17,%hydroxysteroid dehydrogenase, 781 transformation of [4J4C]-testosterone in foetal testis, 749 Ribonucleic acid effect of estrogens on synthesis, on the rat uterus, 445, 503 synthesis and effect of testosterone in rat bone marrow cells, 803
SC 19886 an anti-aldosterone Serum determination
agent, effect of, on sodium
of aldosterone
transport,
by radioimmunoassay,
201
201
Skin aldosterone transport in, of the toad, 111 effect of polyene antibiotics on sodium transport Sodium effect of effect of 137 effect of effect of transport, transport transport transport
aldosterone aldosterone
and aldosterone,
in frog,
on, transport in epitheiia, 557 on content, in the epithelial cells of toad urinary
aldosterone on, and transport of, in rat kidney, 151 aldosterone, on the content of mammalian intestine, action of aldosterone on, 167 and the site of aldosterone effect on, 237 and effect of aldosterone vs. insuhn, 229 and effect of polyene antibiotics on, 121 xix
143
121
bladder,
m
urine determination of aldosterone by radioimmunoassay, 289 human ~F~~~~~ emx&tion of estrogen ~njug~tes in, 31 human pregnancy, identi~ca~on of 5~-androstan~3~,1,7~-diol in, 819 human pregnancy, determination of estrone and estradiol-17fl in, 305 human pregnancy, dete~ination of estriol conjugates in, 807 uterus mouse, e&et of no~th~~~d~~ in the, 871 Fat, enzymic changes in the, 937 Ventral prostate binding of r3~~-~~rot~ron~a~ta~ in cytosol fictions, formation of Sa-dibydrotestosterone receptors, 401
xxi
427
AUTHQR Aakvaag, A. Adferereutz, H. Ahrn~~~ J. A~~ad~~a,N. S. A~~rna~~~E. Ar~~ti~sk~ya~ S, V. As&y, R, Attramadal, A.
375 19,807,943 31 333 26X 791 459 375 289 If 813 219 151
XNDEX Falconi,
c,
Eanestil, D. D. Farooq, A. Fassorra, C. Fazekas, A. 6. Feldman, a. ~~r~~r~~, W. Floch, H, H. Floreasa, E. Franks, D, J. Futlder. s. w.
Caletti, F, Gardi, R. x43 Gel&, C. 567 G~~~~ha~~~ M. E” Ciargi, E. P. 493 ,199, 903,925 325 Godden, u. 819 Gore& T. 837 Gower, D* B. 767 Grant, 3. K. %S9 Grenier, J, 593 Gras, E. 6. Gsrrpide, B 681 G~sta~ss~~, f. A.
837 305 2s5 741 945 773 837 333, 893 725 229, 557
Haksar, A. Hallahan, c. Hampl, a. Handier, J. S. Hanni, S. Hansen, L* Hansson, V. Naukkamaa, M, ~~~~str~rn,B. Neyns, W. Ndzbauer, Me Hrrme, J. R. ~~~ni~~ E. C WorninE;, M. G. Hviid Larsen, E. Xmai, S. Inaba, M,
893
393 871 721 485,661,773 203 877 819 893 643 209,219 831, 933 831, 889,933 543 707 471 767 445 45 471,803 305 11 707 877 847 567 39 137 16i 151 427 631,633 877 593 579,767 643 699 699 111 907 907
907 631 943
Kelhe, A. E. Kimsey, J. Kirsten, E. K&en, R. Kniewafd, Z. Koide, S. S. Kolanowski, J.
31, 275, 357 201 173 173 385 583 349
Laatikainen, T. Lanthier, A. Laumas, v. Laumas, K. R. Lea, 0. A. Leaf, A. Leblanc, H. Lehoux, J.-G. Liang, T. Liao, S. Ludens, J. H. Luisi, M. Lunenfeld, B. Luukkaineo, T.
715 661 871 871 409 237,247 661,683,773 661, 683,773 401 401 193 721 781 631,633
MacDonald, P. C. Ma&night, A. D. C. Madani, C. Makino, I. Mansfield, M. D. Mar&, J. Marocchi, A. Martini, L. Massa, R. Mehdi, A. Z. Menzies, J. A. Mertvetsov, N. P. Mohia, S. Mosher, K. M. Min~eli, J. Mueller, J. R. Mulder, E. Munck, A.
459 237 741 715 333 859 725 385 385 865 43 791 445 567 803 735 601 567
Nakao, T. Newman, G. B. Nielsen, R.
903 289 121
Obenberger, J. Oertel, G. W. G’Malley, B. W. Orloff, J.
39 493, 799, 903, 925 617 137
Pasqualini, J. R. Pelletier, C. Perheentupa, J. Peron, F. G. Piyaaena, R. Pizarro, M. A. Porter, G. A,
Porte, A. M. Preston, A. S.
11 137
Rao, G. S. Tao, M. L. Reponen, A. Riley, W. J. Robertson, D. M. Robidoux, W. F. Robin, M. Robinette, J. A. Rodbard, D. Rogers, A. W. Ros, A, Roaner, W. Rossi, G. L. Rousseau, G.
1 1 943 275,357 275, 357 847 693 735 255 945 741 531 889 219
Salganik, R. I. Salibian, A. Samuel, V. K. Sandor, T. Segal, S. J. Scholler, R. Schoormans, W. Schrader, W. T. Schwarz, B. Shepherd, R. E. Siiteri, P. K. Sjovai, J. Slater, J. D. H. Smals, A. 6. H. Smith, E. R. Snart, R. S. So&!, H. Sommerville, I. F. Spat, A. Stansfield, D. A. Starka, L. Stillwell, R. N. S&a, K. F. Stupnicka, E. Sulimovici, S. Sumida, C.
791
Tagatz, G. E. Tanaka, S. Telegdy, G. Tikkanen, M. J. Toft, P. Taft, D. Tomkins, G. M. Traikov, H. Tuohimaa, P. J. Tveter, K. J. T~~xx&o, J. L.
543 919 715 847 289 349 201 Unhjem, 0. ..I xxm
173 275 485,661,683, 773, 865 503 305 151 617 459 937 459 715 289 325 357 129,937 843 333, 893 755 643 39 699 409 385 781 543 707 445 693 807 43 515 219 767 503 375,427 401 375,427
Van Baelen, H. Van der Molen, H. J. Verdonck, L. Ve~euIen, A. Vibko, R. Votite, 6. L. Watanabe, H. Weniger, J.-P. Wichmann, K. Wiederholt, M.
321 325, 601 421 421 715 161 43 749 633 151
Wilkinson, G. H. Wira, C.
643 567
Yannone, M. E. Young, D. A. Youngiai, E. V. Youssefnejad~an
735 567 919 893
Zeis, A. Zlatikis, A.
749 699
Printed in Great Britain by A. Wheaton & Co., Exeter
OF STEROID
AND MOLECULAR ~
--In__..
BIOCHEMISTRY
BIOLOGY,
._..
THE _
editors-in-chief
J. R. PASQUALINI and R. SCHOLLER
Volume 3 1972 ‘ACC@tDING TO: PROJECT MANAGEM~T E-MAIL DATE: JULY 29,2002 SUBJECT: DPS TITLES OLD TITLE: THE JOURNAL OF STEROID BIOCHEMISTRY NEW TITLE: JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, THE OLD ISSN: NEW ISSN: REMARKS: ARCHIVING DEPARTMENT
*
@
Pergamon
Pl’eSS
Oxford
New York
Brumrchweig
J. R. PASQUALINI and R. SCHOLLER, Foundation for Hormone Research, 26 Boulevard Brune, Paris 14e, France
ASSOCIATE H. H. A. A.
EDITORS
ADLERCREUTZ, Helsinki, Finland H. BREUER,Bonn, Germany J. K. GRANT, Glasgow, Scotland CARSTENSEN, Umea, Sweden H. J. VANDERMOLEN,Rotterdam, Netherlands E, KELLIE, London, England J. K. NORYMBERSKI, Sheffield, England MUNCK, Hanover, N.H., U.S.A. G. W. OERTEL,Mainz, Germany
CORRESPONDING
EDITORS
A. AAKVAAG,Oslo, Norway * A. E. ARG~~ELLES, Buenos Aires, Argentina - M. K. BIRMINGHAM, Montreal, Canada - A. 0. BOSCH,Madrid, Spain * J. P. COGHLAN, Victoria, Australia * C. CONTI,Rome, Italy * E. COUTINHO,Bahia, Brazil + J. CRABBY?, Leuven, BeIgium * 0. DOMINGUEZ,Mexico City, Mexico - K. B. EIK-NES, Los Angeles, Calif., U.S.A. * A. ERCOLI,Milan, Italy - V. A. FRANDSEN,Copenhagen, Denmark - E. GURPIDE,Minneapolis, U.S.A. * R. IGLESIAS,Santiago, Chile - E. V. JENSEN, Chicago, Ill., U.S.A. - M. G. KOLPAKOV,Novosibirsk, U.S.S.R. . S. KORJZNMAN, Iowa. U.S.A. . C. P. LANTOS,Buenos Aires, Argentina - L. MARTINI, Milan, Italy - K. MC~RNS, Gainesville, Fforida, U.S.A. - P. DE MOOR, Leuven, Zurich, Switzerland - M. SA~RAN, Toledo, Ohio, U.S.A. Belgium + J. MUELLER, B. W. O’MALLEY,Nashville, Tennessee, U.S.A. * T. SANDOR,Montreal, Canada * A. SPAT, K. SCHUBERT,Jena, G. D. R. - L. A. SOBREVILLA,Lima, Peru - K. F. ST~A, Bergen, Budapest, Hungary + L. STARKA,Prague, Czechoslovakia Ghent, Belgium - R. Norway - B. TAMAOKI,Chiba-shi, Japan * A. VERMEULEN, VIHKO, Helsinki, Finland
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coNTlxNTs
Number 1
Editors’ Addresses . . . . . . . . . . . Steroid Nomenclature . . . . . . . . . . . Announcement . . . . . . . . . . . . G. S. RAO, MARIE L. RAO and H. BREUER: Studies on a solubilized oestriol 16a-glucuronyltransferase from human liver microsomes . , . . . A. M. PORTO, F. E. BARALLE and E. G. GROS: Biosynthesis of bufadienolides in toads-III. Experiments with [2-14C]mevaIonic acid, [20-14C]3~-hydroxy-5pregnen-20-one and [20-‘*C]cholesterol . . . . . . . A. DESSYPRIS and H. ADLERCREUTZ: Routine clinical determination of testosterone in plasma by competitive-protein-binding . . . . . J. AHMED and A. E. KELLIE: The excretion of oestrogen conjugates in late pregnancy urine . . . . . . . . . . . L. STARKA, R. HAMPL and J. OBENBERGER: Corticosterone in the aqueous humour of the rabbit eye . . . . . . . . . . Short Communication H. WATANABE, P. TOFT and J. A. MENZIES: Conjugation of 2-hydroxyoestrone in rat bife . . . . . . . . . . . General Review D. B. GOWER: 16unsaturated Cl9 steroids; a review of their chemistry, biochemistry and possible physiological role . . . . . . .
i . ..
111 ... VI11
1
11 I9 31 39
43
45
Nun&r 2 Symposium on Aldosterone and Active Sodium Transport Across Epithelia Basel, 23-24 July 1971 Introduction . . . . . Opening remarks: Professors H. TH~LEN, A. WETTSTEIN . . . . Physiological and Morphological
.
.
.
.
.
F. ROLLER, T. ~IC~STEIN .
,
.
.
.
.
V
and .
.
105
Aspects of Aldosterone Action
E. HVXID LARSEN: Characteristics of aldosterone stimulated transport in isolated skin of the toad, Bufo bafo (L-1 . . Discussion by: CrabbB, Hviid Larsen, Morel, Ussing and VoQte . R. NIELSEN: The effect of polyene antibiotics on the aldosterone induced changes in sodium transport across isolated frog skin . Discussion by : Edelman, Leaf, Nielsen, Nutbourne, Ussing and Wiederholt ... 111
111
121
R. S. SNART: The two stage nature of the aldosterone response Discussion by: Edelman, Fanestil, Handler, Snart and Wiederholt J. S. HANDLER, sodium content bladder Discussion by: and Snart
.
.
.
129
A. S. PRESTON and J. ORLOFF: Effect of aldosterone on the and energy metabolism of epithelial cells of the toad urinary Crabbe, De Weer, Edelman, Fanestii, Handler, Jorgensen, Leaf _ . . . . . . . . . . .
C. J. EDMONDS: Effect of aldosterone on mammalian intestine Discussion by: Crabbe, Edelman, Edmonds, Handler, Leaf and Porter
137
.
143
M. WIEDERHOLT, C. BEHN, W. SCHOO~ANS and L. HANSEN: EfIect of aldosterone on sodium and potassium transport in the kidney . . . . Discussion by: Edelman, Fanestil Snart and Wiederholt.
151
C. L. VOUTE, S. HANNI and E. AMMANN: Aldosterone induced mo~hologica~ changes in amphibian epithelia in viva . _ _ Discussion by: Crabbe, Edelman, Leaf, Porter and Vofite .
161
I
Biochemical Asvects of Aldosterone Action I. S. EDELMAN: The initiation mechanism in the action of aldosterone on sodium transport Discussion by: Edelman, Handler and Pas~ualiui . . . . . .
167
E. KIRSTEN, R. KIRSTEN and A. SALIBIAN: A study on the effizct of aldosterone on the extramitochondrial adenine nucleotide system in rat kidney . . Discussion by : CrabbB, De Weer, Edelman, Kirsten, Leaf and Snart .
173
P. L. JMRGENSEN: The role of aldosterone in the regulation of (Na* + KS)ATPase in rat kidney . . . Discussion by: De Weer, Jsrgensen, Kirsten and Wiederholt
181
J. H. LUDENS, J, R. DEVRIES and D. D. FANESTIL: chromatography of aldostreon~binding macromolecules Discussion by: FanestiI, Funder and Snart . . .
.
193
G. A. PORTER and J. KIMSEY: The effect of a new anti-a~dosterone agent SC19886 on aldosterone-stimulated transepithelial sodium transport Discussion by: Edelman, Eigler and Porter . . . . . . _
201
J. W. FUNDER, D. FELDMAN and I. S. EDELMAN: Specific aldosterone binding in rat kidney and parotid . . . . Discussion by: Alberti, Funder, Handler and Rousseau
209
Studies on affinity .
.
.
G. ROUSSEAU, J. D. BAXTER, J. W. FUNDER, TOMKINS : G~ucoco~icoid and miner~oco~~oid
I. S. EDELMAN and G. M. receptors for aldosterone . . . . Discussion by: Funder, Jorgensen, Leaf and Rousseau .
219
J. CRABBE: Hormonal influences on transepithehal sodium transport: aldosterone vs. insulin _ . . Discussion by: Crabbe, Edelman, Hviid Larsen and Ussing .
229
A. LEAF and A. D. C. MACKNIGHT: The site of the aldosterone induced stimulation of sodium transport Discussion by: Alberti, Cereijido, Crabbe, Edelman, Edmonds, Leaf and Porter
237
A. LEAF: Concluding remarks
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iv
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247
Number 3
First International
Symposium of the Journal of Steroid Biochemistry
THE INTERACTION OF STEROIDS WITH MACROMOLECULES Opening remarks by J. R. PASQUALINI Radioimmunassay
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251
and competitive binding assay of steroids
D. RODBARD and K. J. CATT: Mathematical theory of radioligand assays: the kinetics of separation of bound from free Discussion by: Exley, Kellie, Rodbard and Vermeulen . . . . . A. E. KELLIE, V. K. SAMUEL, W. J. RILEY and D. M. ROBERTSON: Steroid glucuronoside-BSA complexes as antigens : the radioimmunoassay of steroid conjugates Discussion by: Adlercreutz, Grant, Kellie, Munck and Siiteri . . . . R. P. EKINS, G. B. NEWMAN, R. PIYASENA, P. BANKS and J. D. H. SLATER: The radioimmunoassay of aldosterone in serum and urine : theoretical and practical aspects Discussion by: Ekins, Pasqualini, Rodbard and Slater . . . . . M. CASTANIER, J. GRENIER and R. SCHOLLER: A direct radioimmunoassay for an estrone and estradiol-l7fl fraction in human urine Discussion by: De Moor and Grenier . . . . . . . H. VAN BAELEN and P. DE MOOR: Steroid-protein interaction as studied by isoelectric focusing Discussion by: Rodbard, Rosner and Van Baelen . . . . . . TN. J. BENRAAD, F. H. DE JONG, H. J. VAN DER MOLEN and A. G. H. SMALS : Comparison and evaluation of a competitive protein binding and a gaschromatographic method for the assay of testosterone in peripheral human plasma Discussion by: CrabbC, Munck and van der Molen . . . . . W. P. COLLINS, M. D. MANSFIELD, N. S. ALLADINA and I. F. SOMMERVILLE : Radioimmunoassay of plasma testosterone Discussion by: Collins, Exley, Fazekas (A. G.), Munck and Rosner . . M. A. PIZARRO and J. KOLANOWSKI: Problems arising from blanks in protein-binding analysis Discussion by: Exley, Haukkamaa, Munck, Piyasena and Pizarro . . W. J. RILEY, E. R. SMITH, D. M. ROBERTSON and A. E. KELLIE: The formation of steroid-BSA antigens via a C-6p-carboxymethyl substituent Discussion by : Exley, Munck and Smith . . . . . . General discussion by: Collins, Cooke, De Moor, Ekins, Exley, Fazekas (A.T.A.; Grant, Kellie, Munck, Rodbard and van der Molen . . . . .
255
275
289
305
321
325
333
349
357 369
Receptors and mechanism of action of androgens-I A. AAKVAAG, K. J. TVETER, 0. UNHJEM and A. ATTRAMADAL: Receptors and binding of androgens in the prostrate Discussion by: Aakvaag, Exley, Grant, Kellie, Liao, Morfin, Pasqualini, Siiteri, Tuohimaa and Vermeulen . . . . . . . . . . V
375
R. MASSA, E. STUPNICKA, Z. KNIEWALD and L. MARTINI : The transformation of testosterone into dihydrotestosterone by the brain and the anterior pituitary Discussion by: CrabbC, De Moor, Exley, Fazekas (A. G.), Fazekas (A. T. A.), Grant, Martini, Munck, O’Malley, Siiteri and van der Molen . . . . S. LIAO, T. LIANG and J. L. TYMOCZKO: Structural recognitions in the interactions of androgens and receptor proteins and in their association with nuclear acceptor components Discussion by: Jensen, Kellie, Liao, Munck and Wira . . . . . 0. A. LEA and K. F. ST0A: The binding of testosterone to different serum proteins : a comparative study Discussion by: Lea, Munck and Rosner . . . . . . . A. VERMEULEN and L. VERDONCK: Some studies on the biological significance of free testosterone Discussion by: CrabbC, De Moor, Martini, Munck, Slater and Vermeulen . V. HANSSON, K. J. TVETER, 0. UNHJEM and 0. DJOSELAND: Studies on the interaction between androgen and macromolecules in male accessory sex organs of rat and man Discussion by: Hansson and Liao . . . . . . . . General discussion by: Aakvaag, Concolino, CrabbC, De Moor, Liao, Martini, Massa, Morfin, Munck, Pasqualini and Tuohimaa . . . . .
Receptors
and mechanism
of action
401
409
421
421 441
of oestrogens-II
E. V. JENSEN, S. MOHLA, T. GORELL, S. TANAKA and E. R. DESOMBRE Estrophile to nucleophile in two easy steps Discussion by: Adlercreutz, Jensen, Munck, Rosner and Wira. . . . P. K. SIITERI, R. ASHBY, B. SCHWARZ and P. C. MACDONALD : Mechanism of estrogen action studies in the human Discussion by: CrabbC, Exley, Grant, Jensen, Munck, O’Malley, Siiteri, Sommerville, Tuohimaa and Wira . , . . . . . . . . J. K. GRANT and ELEONORA P. GIORGI: A dynamic study of androgen binding to macromolecules in the human prostrate Discussion by: Collins, Grant, Jensen, Morfin and Munck . . . . A. G. FAZEKAS and T. SANDOR: Metabolism of androgens by isolated human hair follicles Discussion by: Fazekas (A. G.), Liao, Marlin and O’Malley . . . . G. W. OERTEL and P. BENES: The effects of steroids on glucose-6-phosphate dehydrogenase Discussion by: Fazekas (A. G.), Liao and Oertel . . . . . . D. EXLEY: Specificities of antibodies to oestrogens Discussion by: Adlercruetz, Exley, Grant and Kellie . . . . . P. J. TUOHIMAA, S. J. SEGAL and S. S. KOIDE: Study on uterine ribonucleic acid with estrogenic activity Discussion by: Liao, Munck, O’Malley, Siiteri and Tuohimaa . . . D. TOFT: The interaction of uterine estrogen receptors with DNA . . . General discussion by: Adlercreutz, Exley, Grant, Kellie, Jensen, Liao, Morfin, Munck, O’Malley, Rodbard and Siiteri . . . . . . . vi
385
445
459
471
485
493 497
503 515 523
Receptors and mechanism of action of corticosteroids W. ROSNER: Recent studies on the binding of cortisol in serum Discussion by: Crabbe, De Moor, Holzbauer, Munck, Rosner, Siiteri, Slater and Vermeulen . . . . . . . . . . . .
531
J. R. PASQUALINI, C. SUMIDA and C. GELLY: Mineralocorticosteroid receptors in the foetal compartment Discussion by: Crabbe, Fazekas (A. G.), Martini, Munck, O’Malley, Pasqualini, Slater and Spat . . . . . . . . . . .
543
J. CRABBE: Aldosterone: mechanism of action on isolated sodium-transporting epithelia Discussion by: Adlercreutz, Crabbe and Kellie . . . . . .
557
A. MUNCK, C. WIRA, D. A. YOUNG, K. M. MOSHER, C. HALLAHAN and P. A. BELL: Glucocorticoid-receptor complexes and the earliest steps in the action of glucocorticoids on thymus cells Discussion by: Jensen, Martini, Munck, O’Malley, Pasqualini and Rosner .
567
MARGARETHE
HOLZBAUER:
The association of steroids with blood cells in
vivo
Discussion by: Brinkmann, Carstensen, Holzbauer, Kellie, Martini and Slater .
579
P. DE MOOR, W. HEYNS and R. BOUILLON: Growth hormone and the steroid binding j3-globulin of human plasma Discussion by: De Moor, Martini, Morfin and Vermeulen . . . .
593
Receptors and mechanism of action of progesterone A. 0. BRINKMAN, E. MULDER and H. J. VAN DER MOLEN: Model studies with erythrocytes on the initial steps of cellular uptake and binding of steroids Discussion by: Brinkmann, Holzbauer, Jensen, Oertel, van der Molen and Wira
601
B. W. O’MALLEY and W. T. SCHRADER: Progesterone receptor components: identification of subunits binding to the target-cell genome Discussion by: Crabbe, Jensen, Munck, O’Malley, Siiteri, van der Molen and Wira . . . . . . . . . . . . .
617
M. HAUKKAMAA, 0. KARJALAINEN and T. LUUKKAINEN: In vitro binding of progesterone by the human endometrium during the mestrual cycle . . . and by hyperplastic, atrophic and carcinomatous endometrium
631
M. HAUKKAMAA, K. WICHMANN and T. LUUKKAINEN: Effect of phospholipids on the subcellular distribution of progesterone in pregnant rat myometrium and on protein binding of progesterone Discussion by: Haukkamaa, Jensen, Rosner, Siiteri, Tuohimaa and Wichmann .
633
D. A. STANSFIELD, D. J. FRANKS, G. H. WILKINSON and JANET R. HORNE: Studies in the formation and degradation of adenosine 3’S_cylic monophosphate in corpus luteum Discussion by : Adlercreutz, Cooke, Crabbe, Liao, Munck, Siiteri, Spat, Stansfield and van der Molen . . . . . . . . . . .
643
General discussion by: Crabbe, De Moor, Grant, Kellie, Liao, Martini, Massa, . Morfln, Munck, O’Malley, Pasqualini, Siiteri, van der Molen and Wira .
655
Vii
Number 4 T. SANDOR, A. G. FAZEKAS, J.-G. LEHOUX, HELENE LEBLANC and A. LANTHIER: Studies on the biosynthesis of I&oxygenated steroids from exogenous corticosterone by domestic duck (Anus pZ~#yrhy~cho~) adrenal gland mitochondria . . . . . . . . . . . . H. LEBLANC, J.-G. LEHOUX et T. SANDOR: Etude de la 21-hydroxyiase des . . . glandes surrenale 4 du canard domsetique (Anas platyrhynchos) . G. TELEGDY, M. ROBIN and E. DICZFALUSY: A study of sterol and steroid . synthesis from sodium acetate by human foetal liver preparations W. G. STILLWELL, E. C. HORNING, M. G. HORNING, R. N. STILLWELL and A. ZLATKIS: Characterization of metabolites of steroid contraceptives by gas chromatography and mass spectrometry . . . . . . M. E. GIEBENHAIN, G. E. TAGATZ and E. GURPIDE: Serum levels of unconjugated estetrol-(1,3,5(1 O)-estratrien-3, I%, 16a,I7&tetrol) during human pregnancy . . , . . . . . . . . . T. L~TIK~NEN, J. PERHEENTUPA, R. VIHKO, I. MAKINO and J. SJijVALL: Bile acids and hormonal steroids in biie of a boy with 3/3-hydroxysteroid dehydrogenase deficency . . . . . . . . . M. LUISI and C. FASSORRA: The effect of mesterolone upon the concentration of testosterone in rat testis. . . . . , . . . . . G. CONCOLINO and A. MAROCCHI: A simple procedure for the combined determination of plasma estrogen and androgen concentrations by competitive protein binding analysis . . . . . . . . . . J. R. MUELLER, J. A. ROBINETTE and M. E. YANNONE: An evaluation of “OV” liquid phases for the gas-liquid ~hromato~aphic separation of biologically active steroids . . . . . . . . _ . . . E. M. CHAMBAZ, C. MADANI and A. ROS: TMS-enol-TMS: a new type of derivative for the gas phase study of dihydroxyacetone side chain saturated corticosteroid metabolites . . . . . . . . . . J.-P. WENIGER et A. ZEIS : Mttabolisme de la [4-‘4C]-testostCrone par le testicule embryonnaire de rat cultive in vitro . . . . . . . . Short Communication A. SPAT and SARA J6ZAN: Competitive protein binding assay of corticosterone Errata . . . . . . . . . . . . . Announcements . . . . . . . . . . . .
661 683 693
699
707
715 721
725
735
741 749 755 761 765
Number 5 MARGARETHE HOLZBAUER, MARION K. BIRMINGHAM, A. DE NICOLA, UWA GODDEN and HELL1 TRAIKOV: Steroid secretion by rat . . adrenal glands during the first week following adrenal enucleation . J.-G. LEHOUX, A. G. FAZEKAS, H. LEBLANC, A. CHAPDELAINE and T. SANDOR: Pteridins and steroid hydroxylation. I-The effect of folic acid and aminopterin on the steroid 11/3- and 21-hydroxylation by domestic duck (Anas platyrhymhos) adrenocortical tissue preparations . . . . . , S. SULIMOVICI and B. LUNENFELD: The effect of adenosine 3’,5’-cyclicmonophosphoric acid on the 17~-hydroxysteroid dehydrogenase of rat testis v 1.. Vlll
767
773 781
R. I. SALGANIK, N. P. MERTVETSOV and S. V. ARGUTINSKAYA: The patterns of RNA synthesis and induction of glycogenic enzymes in rat liver under continuous cortisol administration . . . . . . . . P. BENES and G. W. OERTEL: Inhibition of glucosed-phosphate dehydrogenase by steroids. IV-Effects of C,,-steroids upon human placental glucose-6 phosphate dehydrogenase . . . . . . . . . . J. MINGUELL and J. K. GRANT: Rat bone marrow cells: androgen metabolism and actions at the molecular level . . . . . . . . . M. J. TIKKANEN and H. ADLERCREUTZ: A method for the quantitative determination of urinary estriol conjugates in human pregnancy . . . F. BERTHOU, L. BARDOU and H. H. FLOCH: Identification of Sa-androstane3,9,17&diol in human urine . . . . . . . . . F. GALLETTI and R. GARDI: Metabolism of 1-dehydroandrostanes in man. IIMetabolism of 5fl-androst-1-enes. . . . . . . . . A. F. CLARK, R. S. CALANDRA and C. E. BIRD: Kinetics of [3H]-testosterone acetate metabolism in normal young men: effects of medroxyprogesterone (provera) administration . . . . . . . . . . I. BELIC and H. SONIC: Microbial dehydrogenation of tomatidine . . .
791
799
803 807 819 831
837 843
Number 6 A. HAKSAR, F. G. PERON and W. F. ROBIDOUX, JR: The possible function of pyruvate in the steroidogenic response of rat adrenal cell suspensions to ACTH, cyclic-3’,5’-AMP and dibutyryl cyclic-3’,5’-AMP . . . . . . A. M. BONGIOVANNI and J. MARINO: Steroid dehydrogenases in rabbit liver of various developmental stages . . . . . . . . . A. Z. MEHDI and T. SANDOR : Product-precursor relationship during incubation of duck adrenal slices . . . . . . . . . . V. LAUMAS, A. FAROOQ and K. R. LAUMAS : Priming effect of norethynodrel on the uptake of (6,7-3H)-estradiol in the mouse uterus . P. ENEROTH, J.-A. GUSTAFSSON, H. FERNGREN and B: HELLSTROM : Excretion and anticonvulsant activity of steroid hormones in an infant with infantile spasm and hypsarrhytmia treated with excessive doses of ACTH . . G. FALCONI, G. L. ROSSI, A. ERCOLI and R. GARDI: Cyclopentyl ethers of estrogenic steroids. Parallelism between prolonged oral activity and storage in . . . . . . body fat of rats E. YOUSSEFNEJADGN, E. FLORENSA, W. P. COLLINS and I. F. SOMMER: VILLE: Radioimmunoassay of plasma progesterone . . . . . G. W. OERTEL and P. BENES: Inhibition of glucose&phosphate dehydrogenase by steroids. VI-Effects of new synthetic dehydroepiandrosterone conjugates upon the activity of human placental glucosed-phosphate dehydrogenase . . M. INABA, K. KAMATA, S. IMAI and T. NAKAO: Studies on secretion mechanism of adrenal corticosterone: adrenal subcellular distribution of corticosterone and its changes on hypophysectomy and ACTH administration . E. V. YOUNGLAI and C. PELLETIER: A competitive protein-binding method for the measurement of progesterone in human amniotic fluid . . . . P. BENES and G. W. OERTEL: On steroid conjugates in plasma. XXIII-Biosynthesis of steroid sulfatide from a cytidine phosphosulfate steroid complex . ix
847 859 865 871
877
889 893
903
907 919 925
F. GALLETTI and R. GARDI: Metabolism of I-dehydroandrostanes in man. III-Metabolism of 17/3-hydroxy-Sa-androst-1-en-3-one, 17/3-(l’-methoxycyclohexyloxy)-5a-androst-I-en-3-one (mesabolone) and Sa-androst-1-ene-3,17-dione R. E. SHEPHERD and R. S. SNART: Oestradiol induced respiratory and enzymic changes in rat uterus . . . . . . . . . . Short Communications A. DESSYPRIS, A. REPONEN, S.-L. KARONEN and H. ADLERCREUTZ: . Technical improvements to a method for determination of testosterone . J. CHAMBERLAIN and A. W. ROGERS : The appearance of dihydrotestosterone3H in the diencephalon of neonatal rats after subcutaneous injection of testosterone-3H . . . . . . . . . . . .
933 937
943
945
ACTH (adrenocorticotrophin) effect on corticosterone secretion in hypophysectomized rats, 907 effect on steroid hormone excretion, on an infant with h~sa~hytmia and infantiie spasm, 877 effect on steroidogenesis, in rat adrenal cell suspensions, 847 Adenine nucleotide effect of aldosterone on, in extramitochondrial fraction of rat kidney, 173 Adenosine 3’,5’-cyclic monophosphate effect on 17jLhydroxysteroid dehydrogenase, in rat testis, 781 formation and degradation of, in rat corpus luteum, 643 Adrenal(s) biosynthesis of l&oxygenated steroids, in duck, 661 effect of folic acid and aminopterin on steroid ll/% and 21-hydroxylations by duck, 773 effect of pyruvate in steroidogenesis of rat, 847 steroid secretions in rat, following enucleation, 767 subcellular distribution of corticosterone in hypophysectomized rat, 907 transformation of [4-‘4C]progesterone in duck, 865 Afhnity chromatography isolation in human serum of CBG (corticosteroid binding globulin) by, 53 1 studies on aldosterone-binding macromolecules, 193 Aldosterone and morphological changes in amphibian epithelia in vim, 161 and polyene antibiotics, effect of, on sodium transport in frog skin, 121 and two specific binding sites in isolated toad bladder, 129 binding macromo~~ules and studies by affinity chromatography, 193 biosynthesis by adrenais of ducks, 661 effect of a new anti-aldosterone agent: SC 19886 on sodium transport, 201 effect of, in adenine nucleotide system in rat kidney, 173 effect of, on sodium and potassium transport in the rat kidney, 151 effect of, on sodium transport in isolated skin of toad, 111 effect of, on sodium content in epithelial cells of the toad bladder, 137 effect of, on sodium content on mammalian intestine, 143 formation of receptors and mechanism of action in the kidney of adrenalectomized rats, 167 glucocorticoid and mineralocorticoid receptors for, 219 mechanism of action of, on target cells in isolated epitheha, 557 radioimmunoassay of, in serum and urine, 289 receptors, formation in the foetal kidney of guinea pig, 543 role of, in the regulation of (Na+ + K+)-ATPase in the rat kidney, 181 site of action and stimulation on sodium transport, 237 specific bindings in rat kidney and parotid, 209 vs. insulin influences on transepithelial sodium transport, 229 xi
Amniotic fluid measurement
of progesterone by competitive protein-binding method in humans, 919 Androgens binding to macromolecules in human prostate, 471 determination of plasma by competitive protein binding, 725 macromolecule interaction in male accessory sex organs of rat and man, 427 metabolism and actions at the molecular level of, in rat bone marrow cells, 803 metabolism of, by isolated human hair follicles, 485 receptors and binding of, in the prostate, 375 receptor proteins and nuclear acceptors, 401 5fi-Androst-1-enes metabolism in humans, 831 Sa-Androst-1-ene-3,17-dione metabolisms in humans, 933 Sa-Androstane-3j3,17@diol identification of, in human urine, 819 Androstene-3,17-dione metabolism in human male hair follicles, 485 Antibodies specificities to estrogens, 497 Anti-aldosterone effect of SC 19889 compound (a 12a, 13&etiojervane derivative) on aldosteronestimulated transepithelial sodium transport, 201 Aqueous humour corticosterone identification in, of the rabbit eye, 39
Bile 2-hydroxyoestrone conjugation in, 43 Bile acids in the bile of a boy with 3fl-hydroxysteroid dehydrogenase deficiency, 715 Binding aldosterone, in rat kidney and parotid, 209 determination of plasma estrogens and androgens by protein, method, 725 determination of plasma testosterone by competitive protein, method, 19 of androgens to macromolecules in the human prostate, 375, 471 of cortisol in serum, 531 of progesterone, in chick oviduct, 617 of progesterone, in human endometrium, 631 of progesterone in the myometrium of pregnant rats, 633 of steroids to erythrocytes, 601 of testosterone to different serum proteins, 409 protein, and assay for corticosterone, 755 Blood cells “in vivo” association of steroids with, 579 Bone marrow cells metabolism of androgens at the molecular level, in rats, 803 Brain transformation of testosterone into dihydrotestosterone by, 385 xii
Bufadienolides biosynthesis of, in toads, 11 CIa steroids effect of, on human placental glucose-6-phosphate dehydrogenase, 799 C19 steroids conjugates, in the bile of a boy with 3/3-hydroxysteroid dehydrogenase deficiency, 715 16unsaturated, chemistry, biochemistry and physiological role of, 45 CzI steroids conjugates, in the bile of a boy with 3,Shydroxysteroid dehydrogenase deficiency, 715 Cdj3-carboxymethyl steroids formation of bovine serum albumin antigens, via, 357 Chick oviduct receptors of progesterone, 617 Cholesterol and biosynthesis of bufadienolides, in toads, 11 Competitive protein binding determination of estradiol, estrone, testosterone and androstenedione in human plasma, 725 determination of progesterone by, in human amniotic fluid, 919 determination of testosterone by, in peripheral human plasma, 707 Contraceptives characterization of metabolites by gas chromatography and mass spectrometry, 699 Corticosteroids metabolism determination by gas chromatography, 741 Corticosterone association to blood cells, 579 biosynthesis of 1&oxygenated steroids from, in domestic duck, 661 determination by competitive protein binding in rat plasma, 755 in the aqueous humour of the rabbit eye, 39 studies on mechanism of secretion in rats, 907 transformation into l&oxygenated steroids by the adrenals of domestic duck, 661 Cortisol association to blood cells, 579 binding of, in human serum, 531 binding to transcortin, 321. effect of, on RNA synthesis in rat liver, 791 receptors in thymus cells, 567 Cyclic-3’,5’-AMP and corticosterone biosynthesis, in rat adrenals, 847 Cyclopentyl ethers studies on biological activities of estrogenic steroid derivatives, 899 Dehydroepiandrosterone sulphate metabolism in human male hair follicles, 485 transformation of, by mitochondrial preparation ... xl11
of guinea pig liver, 925
gIu~ose-6-~hospha~e, effect of C, *-steroids upon human placenta, 799 3~-hydroxys~eroid deficiency in a boy, 715 17jLhydroxysteroid, effect of adenosine 3’$‘-cychomonophosphoric acid on, of rat testis, 781 Dehydrogenation of toma~idine by the microorganism: ~ucffrd~~restrictz.t.s, 843 Deoxycorticosterone association to blood cehs, 579 secretion by the rat adrenal glands, 767 5u-~ihydrotes~os~~rone binding in epididymis of adult castrated rats, 427 birding to specific receptors in the mi~rosoma1 or cytosohc fractions of rat ventral prostate, 401 in the diencephalon of neonatal rats after injection of testosterone, 945 transformation of testosterone into, by the brain, 385 Dimethisterone (6a-methyl-l7u-(l-propynyl)-I7~-hydroxy-4-androsten-3-one) metabolism in humans, 699 DNA and progesterone binding in chick oviduct, 617 interaction of uterine estrogen receptors with3 515 Duck biosy~~esis of lo-oxygena~d steroids by the adrena& of, 661 effect of folic acid and am~iopte~n on I I@- and Al-bydroxyIa~ions of&e adrenal glands, 773 transformation of [4-r4Cj-progesterone, by the adrenals of, 865
Endometrium in V&O b~uding of progesterone by the human, 631 ~~idid~is binding to Sa-dihydrotestosterone, in adult castrated rats, 427 Epi-estriol 16~-gIuc~r~~oside urinary excretion in late pregnancy, 31 E~i~eIi~ o&Is ef%ectof dldosterone, on sodium content, in toad urinary bladder, 137 Erythrocytes studies on steroid binding, 601 Estetro1-(1,3,5(10)-estratrien-3,15a,16~,17~-tetra1) in human pregnancy plasma, 707 Estradid dete~natiou by competitive protein binding in human pfasma, 725 effect of, in enzymic changes in rat uterus, 937 effect of ~~re~ynodrel on the uptake of, in mouse uterus, 871 17&gIucuronoside, urinary excretion in late pregnancy, 31 radjoi~~~oassay for, in human urine, 305
and ~~-giucu~ony~~ansferase from liver microsomes, 1 dete~i~at~an of conjugates, in human prqyxmcy urine, 807 3-glucuro~~side, urinary excretion in late pregnancy, 31 lo-gluc~ro~oside, urinary excretion in late pregnancy, 31 3-s~~hate-l6u_gluc~onoside, urinary excretion in late ~r~g~an~~ 31 ~s~ogens and antibody s~c~~ci~es~ 497 and uterine ribonucleic acid in rats, 503 c~njugat~s~ urinary excretion in late pre~a~cy~ 3% determination by competitive protein binding, 72.5 specific receptors and mechanism of formative in the rat uterus, 445 studies of mecb~sm of action, in human e~dome~~~ $59 uterine receptor and interactions with DNA, 515 Estrone 3-g~ucuron~side, excretion in late pregnancy, 31 lfia-hydroxy, 16~-glucura~~side, urinary excretion in late pregnancy, 31 16u~hydroxy-3-sulFha~-l6~-glucuronoside~ urinary excretion in late pregnancy, 31 ra~o~~~u~s~y for, in human urine, 305 Foetal compliment ~neraloc~~costeroid receptors in the, 543 Foetal liver sterol and steroid synthesis from sodium a&ate by+ 693 Fohc acid effect of, on steroid Ilp- and 21-hydroxylations by domestic duck, 773 Gas chromatography characterization of contraceptive metabolites, by, 699 de~~~a~o~ of estrogen conjugates by* in urinary human ~~~cy~ 807 d~r~natio~ of ~e~~~~~erQ~~ by, in per~hera~ human plasma> 325 of corticosteroid metabohtes, 741 separation of biologically active steroids, 735 &Globulin steroid binding, in human plasma, 593 ~~ucoco~coid~s~ receptors in thymus ceh, 567 Glucose-~~hosphate d~ydr~ge~ase effects of CIs steroids, upon human placenta, 799 effects ofnew synthetic dehydroepiandrosterot conjugates upon human placenta, 903 effects of difierent steroids on, 493 G~~c~onos~de*~SA (bovine serum ~b~rn~n~ eomp~ex~ used as antigens for radi~i~unoassay of steroid conjugates, 275 Glycogenic enzymes induction of? by cortisol in rat liver, 791 Growth ~o~~~e effect on the binding of different steroids to &#obuhn, 493 xv
Human liver microsomes studies on I&-glucuronyltransferase, I Hydrocortisone (see Cortisol) 1$~~ydroxy-corticost~rone association to blood cells, 579 biosynthesis by the adrenal of duck, 661 1%Hydroxy-deoxycorticosterone secretion by the rat adrenal glands, 767 2-Hydroxyestrone 2-glucuronoside ~o~~~gat~o~ of, in rat bite, 43 16u-~ydro~y~strone 3-gfucuronoside, urinary excretion in late pregnancy, 31 3/3-Hydroxy-5-pregnen-20-one transformation to bufadienolides in toads, 1I I7/?-Hydroxy-Sa-androst-1-en-3-one metabolism of, in man, 933 21-~ydroxylase studies in the adrenals of ducks, 683 11,G-and 21-Hydroxylation by adrenals of domestic duck, effect of folic acid on, 773 3~-~ydroxysteroid dehydrogen~e deficiency of activity, in a pa~o~o~~~a~ case, 715 ~7~~H~dr~xysteroid dehydrogemtse effect of adenosine 3’5’-~ycli~~monophosphoric acid on, in rat testis, 781 Hyperpfastic e~dometr~~m binding of progesterone in, 631 Insulin vs. aldosterone, influences on transepithelial sodium transport, Isoelectric focusing application to steroid-protein interaction, 321
229
Kidney aldosterone binding in rat, and parotid, 209 effect of aldosterone on sodium and potassium transpo~ in rat, 151 effect of aldosterone on the extr~~to~hond~a~ adenine nucteotide in rat, 173 foe&i guinea pig, and mineraXocorticosteroid receptors, 543 role of aldosterone activity on @a* -I- K?J-ATPase, in rat, 181 Liver and steroid dehydrogenase activities in rabbit, 859 human foetal, bios~~esis of sterols and steroids, 693 oestriol, IGa-glucuronyltransferase in microsomes, in humans, 1 MaIe accessory sex organs
androgens and macromolecule interac~ons, of rat and man, 427 ~amma~ian intestine effect of atdosterone on, 143 xvi
Mass spectrometry
characterization of contraceptive metabolites by, 699 M~~~x~ro~sterone acetate elect of [3H]testosterone rnetab~~~~s in normal young men, 837 Menstrual cycle beading of progesterone in the human endometrium~ during, 631 Mesabolone (I?@-(1,’me~oxy-~yclohe~~loxy~“5~-androst-I-en-3-o~e~ metabolism of, in man, 933 Mesterolo~e effect of, upon ~on~ntmtion of testosterone in rat testis, 721 [2- i %I J-Mevalo~~ acid and biosynthesis of b~fadieoo~des in toads, I I M~ne~~I~co~ticosteroids receptors in the foetal compartment, 543 receptors for aldosterone, 219 Mouse effect of nu~~yn~rel on the uptake of [6,~-3H]-estradiol in the uterus of, 8% Myomet~ium effect of phosphol~ids on the su~ll~lar distribntio~ of progesterone in pregnant rat, 633 Neonatal rats ~ydrotestosteron~~3H] in the diencephalon of, 945 Norethisterone ft?u~thynyl-I7~-hyd~oxy-4-estren-3-one) metabolism in humans, 699 Norethynodrel effect of, on the uptake of [6,~-~H~~estradiol in the mouse uterus, 87X Oestriol see estriol Oestrogen see estrogen Oestradiol see estradiol I$-Uxygenated steroids biosynthesis of, by the Adonis
of domestic duck, 661
effect of, on progesterone subcellular distribution in pregnant rat myometrium, 633 Pituitary transformation of testosterone into dihydr~testosterone in anterior, 385 Placenta effects of CIS steroids, upon glu~ose~6-phosphate dehydroge~ase in humans, 799 elect of new synthetic deh~d~iandrosterone ~o~jngates upon glucose-6phosphate dehydrogen~~ in humans, 903 xvii
Plasma assay of testosterone by gas chromatography and competitive protein binding method in peripheral human, 325 determination of estrogens and androgens by competitive protein binding, 725 determination of testosterone in, by competitive protein binding method, 19 growth hormone and steroid binding /LB-globulinin human, 593 protein binding in peripheral human, 325 testosterone evaluation by radi~immu~oass~y, 333 effect of, on aldosterone and sodirrm transport, in frog skin, f2I effect of aldost~rone on transport in rat kidney, 151 effect of aldosterone, on the content of mammalian intestine, 143 Pregnancy and effect of phospholipids on the subceilular distribution of progesterone in rats, 633 and estetrol-(1,3,5(10)-estratrien-3, I&, 1tia, 17,%tetrol) evaluation in humans, 707 determjnation of urinary estriol conjugates in human, 807 urinary excretion of estrogen conjugates, 31 [20- *4~~-~r~gneno~one and biosynth~~s of bufad~e~o~ides, in toads, If association to bfood eelfs, 579 z&m binding of, in human e~d~metrjnrn during menstrual q&e, 631 measurement of, by competitive protein binding in human amniotic fluid, 919 r~~ioimmunoassay of, in plasma, 893 receptors, in target cell gename, 617 subcellular distribution of, in pregnant rat myometrium, 633 in
Prostate androgen binding to ma~romolec~Ies in the human, 471 receptors of androg~ns in the rat, 375 Proteins and androgen receptors in the prostate, 401 and steroids binding, studied by isoelectric fo~us~~~~ 321 binding to progesterone, in pregnant rat myometrium, 633 binding to corticosteroids and progesterone, and problems arising from blanks, 349 binding to testosterone, 19, 409 R&dins and steroid hydroxylations,
773
Rabbit(s) steroid dehydrogenase a~i~viti~s in the liver of, 859 ~orticosterune in the aqueous humour of, 39 _I. XWU
Radioimmunoassay evaluation of estriol and estetrol in pregnancy urine by, 707 of aldosterone in urine and plasma, 289 of plasma progesterone, 893 of testosterone in plasma, 333 studies between steroid and macromolecules of estrone and estradiol urine, 305 theoretical model and mathematical consideration for the radioligand,
in human 255
Rat(s) bile and conjugation of 2-hydroxyestrone, 43 body fat and effect of estrogenic steroids on, 889 bone marrow cells, androgen metabolism and actions at the molecular level, 803 ~3H]-dihydrotestosterone in the diencephalon of neonatal, 945 effect of mesterolone upon testosterone on, 721 effect of phospholipids in the myometrium of, during pregnancy, 633 interactions between androgens and macromolecules in male sex organs of, 427 kidney and effect of aldosterone in the regulation of the (Na+ + K+), 181 kidney and parotid aldosterone binding in, 209 kidney and effect of ddosterone on adenine nucleotide system in, 173 liver, effect of cortisol in glycogenic enzymes, 791 studies on mechanisms of corticosterone secretion, 907 testis and effect of adenosine 3’5’-cyclic-monophosphoric acid on 17,%hydroxysteroid dehydrogenase, 781 transformation of [4J4C]-testosterone in foetal testis, 749 Ribonucleic acid effect of estrogens on synthesis, on the rat uterus, 445, 503 synthesis and effect of testosterone in rat bone marrow cells, 803
SC 19886 an anti-aldosterone Serum determination
agent, effect of, on sodium
of aldosterone
transport,
by radioimmunoassay,
201
201
Skin aldosterone transport in, of the toad, 111 effect of polyene antibiotics on sodium transport Sodium effect of effect of 137 effect of effect of transport, transport transport transport
aldosterone aldosterone
and aldosterone,
in frog,
on, transport in epitheiia, 557 on content, in the epithelial cells of toad urinary
aldosterone on, and transport of, in rat kidney, 151 aldosterone, on the content of mammalian intestine, action of aldosterone on, 167 and the site of aldosterone effect on, 237 and effect of aldosterone vs. insuhn, 229 and effect of polyene antibiotics on, 121 xix
143
121
bladder,
m
urine determination of aldosterone by radioimmunoassay, 289 human ~F~~~~~ emx&tion of estrogen ~njug~tes in, 31 human pregnancy, identi~ca~on of 5~-androstan~3~,1,7~-diol in, 819 human pregnancy, determination of estrone and estradiol-17fl in, 305 human pregnancy, dete~ination of estriol conjugates in, 807 uterus mouse, e&et of no~th~~~d~~ in the, 871 Fat, enzymic changes in the, 937 Ventral prostate binding of r3~~-~~rot~ron~a~ta~ in cytosol fictions, formation of Sa-dibydrotestosterone receptors, 401
xxi
427
AUTHQR Aakvaag, A. Adferereutz, H. Ahrn~~~ J. A~~ad~~a,N. S. A~~rna~~~E. Ar~~ti~sk~ya~ S, V. As&y, R, Attramadal, A.
375 19,807,943 31 333 26X 791 459 375 289 If 813 219 151
XNDEX Falconi,
c,
Eanestil, D. D. Farooq, A. Fassorra, C. Fazekas, A. 6. Feldman, a. ~~r~~r~~, W. Floch, H, H. Floreasa, E. Franks, D, J. Futlder. s. w.
Caletti, F, Gardi, R. x43 Gel&, C. 567 G~~~~ha~~~ M. E” Ciargi, E. P. 493 ,199, 903,925 325 Godden, u. 819 Gore& T. 837 Gower, D* B. 767 Grant, 3. K. %S9 Grenier, J, 593 Gras, E. 6. Gsrrpide, B 681 G~sta~ss~~, f. A.
837 305 2s5 741 945 773 837 333, 893 725 229, 557
Haksar, A. Hallahan, c. Hampl, a. Handier, J. S. Hanni, S. Hansen, L* Hansson, V. Naukkamaa, M, ~~~~str~rn,B. Neyns, W. Ndzbauer, Me Hrrme, J. R. ~~~ni~~ E. C WorninE;, M. G. Hviid Larsen, E. Xmai, S. Inaba, M,
893
393 871 721 485,661,773 203 877 819 893 643 209,219 831, 933 831, 889,933 543 707 471 767 445 45 471,803 305 11 707 877 847 567 39 137 16i 151 427 631,633 877 593 579,767 643 699 699 111 907 907
907 631 943
Kelhe, A. E. Kimsey, J. Kirsten, E. K&en, R. Kniewafd, Z. Koide, S. S. Kolanowski, J.
31, 275, 357 201 173 173 385 583 349
Laatikainen, T. Lanthier, A. Laumas, v. Laumas, K. R. Lea, 0. A. Leaf, A. Leblanc, H. Lehoux, J.-G. Liang, T. Liao, S. Ludens, J. H. Luisi, M. Lunenfeld, B. Luukkaineo, T.
715 661 871 871 409 237,247 661,683,773 661, 683,773 401 401 193 721 781 631,633
MacDonald, P. C. Ma&night, A. D. C. Madani, C. Makino, I. Mansfield, M. D. Mar&, J. Marocchi, A. Martini, L. Massa, R. Mehdi, A. Z. Menzies, J. A. Mertvetsov, N. P. Mohia, S. Mosher, K. M. Min~eli, J. Mueller, J. R. Mulder, E. Munck, A.
459 237 741 715 333 859 725 385 385 865 43 791 445 567 803 735 601 567
Nakao, T. Newman, G. B. Nielsen, R.
903 289 121
Obenberger, J. Oertel, G. W. G’Malley, B. W. Orloff, J.
39 493, 799, 903, 925 617 137
Pasqualini, J. R. Pelletier, C. Perheentupa, J. Peron, F. G. Piyaaena, R. Pizarro, M. A. Porter, G. A,
Porte, A. M. Preston, A. S.
11 137
Rao, G. S. Tao, M. L. Reponen, A. Riley, W. J. Robertson, D. M. Robidoux, W. F. Robin, M. Robinette, J. A. Rodbard, D. Rogers, A. W. Ros, A, Roaner, W. Rossi, G. L. Rousseau, G.
1 1 943 275,357 275, 357 847 693 735 255 945 741 531 889 219
Salganik, R. I. Salibian, A. Samuel, V. K. Sandor, T. Segal, S. J. Scholler, R. Schoormans, W. Schrader, W. T. Schwarz, B. Shepherd, R. E. Siiteri, P. K. Sjovai, J. Slater, J. D. H. Smals, A. 6. H. Smith, E. R. Snart, R. S. So&!, H. Sommerville, I. F. Spat, A. Stansfield, D. A. Starka, L. Stillwell, R. N. S&a, K. F. Stupnicka, E. Sulimovici, S. Sumida, C.
791
Tagatz, G. E. Tanaka, S. Telegdy, G. Tikkanen, M. J. Toft, P. Taft, D. Tomkins, G. M. Traikov, H. Tuohimaa, P. J. Tveter, K. J. T~~xx&o, J. L.
543 919 715 847 289 349 201 Unhjem, 0. ..I xxm
173 275 485,661,683, 773, 865 503 305 151 617 459 937 459 715 289 325 357 129,937 843 333, 893 755 643 39 699 409 385 781 543 707 445 693 807 43 515 219 767 503 375,427 401 375,427
Van Baelen, H. Van der Molen, H. J. Verdonck, L. Ve~euIen, A. Vibko, R. Votite, 6. L. Watanabe, H. Weniger, J.-P. Wichmann, K. Wiederholt, M.
321 325, 601 421 421 715 161 43 749 633 151
Wilkinson, G. H. Wira, C.
643 567
Yannone, M. E. Young, D. A. Youngiai, E. V. Youssefnejad~an
735 567 919 893
Zeis, A. Zlatikis, A.
749 699
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