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4703010 Electrolytic bioreactor assembly and method
90
PATENT ABSTRACTS
tivities of EPO of human or monkey species origins. Disclosed also are chemically synthesized polypeptides sharing the biochemical and immunological properties of EPO. Also disclosed are improved methods for the detection of specific single stranded polynucleotides in a heterologus cellular or viral sample prepared from, e.g., DNA present in a plasmid or viralborne cDNA or genomic DNA library.
infected cell culture is stained with a phenanthridium dye. Only infected cells absorb the dye. Dead cells are counted with a cytofluorograph indicating virion concentration. The two tests combined discriminate between teratogens and carcinogens. Toxins which are teratogens reduce the number of infective virus, whereas carcinogens not only reduce the virus number, but increase the number of mutants.
4703010 ELECTROLYTIC BIOREACTOR ASSEMBLY AND METHOD Stanley B Yunker, John M Radovich assigned to The Board of Regents for the University of Oklahoma An electrolytic bioreactor, assembly and method for growing organisms capable of oxidizing metal sulfides. The reactor comprises a vessel defilaing an anode chamber for housing an anode, and a cultivation chamber, for housing a cathode and a culture solution, a selective barrier between such chambers, which is impermeable to the organisms and to cations, and a receptacle defining a pH control chamber for receiving samples of culture solution from the cultivation chamber so that such samples are insulated from electric current within the cultivation chamber. Preferably, the capacity of the anode chamber is about 1~ to about 3~o of the cultivation chamber. In accordance with the method of the present invention during operation of the reactor assembly, the pH of the culture solution is controlled by circulating samples through the pH control chamber.
4704356 METHOD OF DIAGNOSING CARTILAGE TISSUE ABNORMALITIES Eugene Thonar assigned to Rush-PresbyterianSt Luke's Medical Center It is found that abnormal levels of keratan sulfate in the peripheral blood are indicative of abnormalities of cartilage or cartilage-like tissues. Specifically, elevated levels of keratan sulfate in the peripheral blood plasma or serum are indicative of osteoarthritis and either substantially complete absence of or very elevated levels of keratan sulfate in the peripheral blood are indicative of muscular dystrophy and related disorders. The level of keratan sulfate in the peripheral blood is determined by an immunoassay using a monoclonal antibody. Preferably the immunoassay employs a colorimetric reporter system.
4704357 47~3~ VIRION ASSAY METHOD FOR USE IN IN VITRO SCREENING OF TERATOGENS AND CARCINOGENS Stephen Keller assigned to University of Cincinnati A cytofluorograph is used to quantify poxvirus concentration. Virions producing a known cell bound antigen are added to a cell culture. The antigen is fluorescently labeled and the infected cells are counted cytofluorographically. Certain mutated virions do not produce the labeled antigen and are not counted. Alternately, the virion
I M M O R T A L I Z E D TLYMPHOCYTE CELL LINE FOR TESTING HTLV-III INACTIVATION Hiroak Mitsuya, Samuel Broder assigned to United States of America as represented by the Department of Health and Human Services This invention is an immortalized T-cell clone, designated ATH8, which is highly sensitive to the cytopathic effect of HTLV-II1. The ATH8 Tcell clone is used in mass screening systems to rapidly and easily determine the in vitro caPacity of new drugs or other agents to inactivate or inhibit HTLV-III or related cytopathic retroviruses.
PATENT ABSTRACTS
tivities of EPO of human or monkey species origins. Disclosed also are chemically synthesized polypeptides sharing the biochemical and immunological properties of EPO. Also disclosed are improved methods for the detection of specific single stranded polynucleotides in a heterologus cellular or viral sample prepared from, e.g., DNA present in a plasmid or viralborne cDNA or genomic DNA library.
infected cell culture is stained with a phenanthridium dye. Only infected cells absorb the dye. Dead cells are counted with a cytofluorograph indicating virion concentration. The two tests combined discriminate between teratogens and carcinogens. Toxins which are teratogens reduce the number of infective virus, whereas carcinogens not only reduce the virus number, but increase the number of mutants.
4703010 ELECTROLYTIC BIOREACTOR ASSEMBLY AND METHOD Stanley B Yunker, John M Radovich assigned to The Board of Regents for the University of Oklahoma An electrolytic bioreactor, assembly and method for growing organisms capable of oxidizing metal sulfides. The reactor comprises a vessel defilaing an anode chamber for housing an anode, and a cultivation chamber, for housing a cathode and a culture solution, a selective barrier between such chambers, which is impermeable to the organisms and to cations, and a receptacle defining a pH control chamber for receiving samples of culture solution from the cultivation chamber so that such samples are insulated from electric current within the cultivation chamber. Preferably, the capacity of the anode chamber is about 1~ to about 3~o of the cultivation chamber. In accordance with the method of the present invention during operation of the reactor assembly, the pH of the culture solution is controlled by circulating samples through the pH control chamber.
4704356 METHOD OF DIAGNOSING CARTILAGE TISSUE ABNORMALITIES Eugene Thonar assigned to Rush-PresbyterianSt Luke's Medical Center It is found that abnormal levels of keratan sulfate in the peripheral blood are indicative of abnormalities of cartilage or cartilage-like tissues. Specifically, elevated levels of keratan sulfate in the peripheral blood plasma or serum are indicative of osteoarthritis and either substantially complete absence of or very elevated levels of keratan sulfate in the peripheral blood are indicative of muscular dystrophy and related disorders. The level of keratan sulfate in the peripheral blood is determined by an immunoassay using a monoclonal antibody. Preferably the immunoassay employs a colorimetric reporter system.
4704357 47~3~ VIRION ASSAY METHOD FOR USE IN IN VITRO SCREENING OF TERATOGENS AND CARCINOGENS Stephen Keller assigned to University of Cincinnati A cytofluorograph is used to quantify poxvirus concentration. Virions producing a known cell bound antigen are added to a cell culture. The antigen is fluorescently labeled and the infected cells are counted cytofluorographically. Certain mutated virions do not produce the labeled antigen and are not counted. Alternately, the virion
I M M O R T A L I Z E D TLYMPHOCYTE CELL LINE FOR TESTING HTLV-III INACTIVATION Hiroak Mitsuya, Samuel Broder assigned to United States of America as represented by the Department of Health and Human Services This invention is an immortalized T-cell clone, designated ATH8, which is highly sensitive to the cytopathic effect of HTLV-II1. The ATH8 Tcell clone is used in mass screening systems to rapidly and easily determine the in vitro caPacity of new drugs or other agents to inactivate or inhibit HTLV-III or related cytopathic retroviruses.