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4859603 Personal diagnostic kit
PATENT ABSTRACTS 4859584 CELL GROWTH RATE DETERMINATION BY MEASUREMENT OF CHANGES CYANINE DYE LEVELS IN PLASMA MEMBRANES
IN
Paul Horan, Bruce D Jensen, Sue Slezak assigned to SmithKline Beckman Corporation Methods for determining growth rate of cells growing in vivo and in vitro. Cells are labelled with cyanine dyes and changes in plasma membrane cyanine dye levels are used to determine growth rate. Cell growth rate determinations are utilized to monitor transplanted bone marrow cell engraftment and post-surgical corneal epirhea[ cell growth. The invented methods also are used to determine tumor cell sensitivity to cancer therapeutic agents, yeast sensitivity to antifungal agents, and bacteria sensitivity to antibacterial agents. 4859585 IN-VITRO METHODS FOR IDENTIFYING COMPOSITIONS WHICH ARE AGONISTS AND ANTAGONISTS OF ESTROGENS Carlos Sonnenschein, Ana Soto assigned to Trustees of Tufts College The present invention provides two general protocols by which a substance may be identified and characterized as an estrogen agonist and/or an estrogen antagonist. The protocols are invitro methods which utilize estrogen dependent cells in culture and a medium comprising an inhibitor endogenous to the sera of adult humans which is able to prevent the proliferation of cultured cells in-vitro. The methodology is rapid, reproducible, accurate and provides the major advantage of being able to screen large numbers of substances for their primary or secondary side effects in a large variety of medical and environmental applications. 4859586 DEVICE FOR CULTIVATING BACTERIA Eliahu Eisenberg, Reuven Padova, Avner Shenfeld, Tsvi Hirshfeld, Jacob Stein, Tel Aviv, Israel
409
There is provided a hermetically closed culture vessel in which miroorganisms are cultivated simultaneously or consecutively on a liquid cuiture medium and on one or more solid culture media. The culture vessel is transparent, and contains in its lower part a liquid culture medium. From the cap, closing the vessel, there extends downwardly an elongated support for the solid culture medium or media, which is essentiaUy parallel with the central axis of the vessel, which terminates above the liquid culture medium. The growth on the solid culture medium is conveniently inspected from the side of the culture vessel.
4859603 PERSONAL
DIAGNOSTIC
KIT
Charles M Dole, Gary L Webster assigned to Dole Associates Inc A personal, disposable hand held diagnostic kit, having a specimen support member such as a membrane, wafer on the like including a plurality of liquid receptacles with means for applying the liquids to said support member in sequence for relatively immediate observation of reaction and ultimate diagnosis.
4859766 PURIFIED
APPETITE-
REGULATING SUBSTANCES OF BIOLOGICAL ORGIN, THEIR ANTIBODIF.~ IMMUNOCOMPLEXES OF THE APPETITE-REGULATING SUBSTANCES FORMED WITH THE ANTIBODIES AND PROCESSES FOR PREPARING SAM[E Jozse Knoll, Sando Harmath, Janos Nagy, Berta Knoll, Budapest, Hungary assigned to Richter G-edeon Vegyeszeti The selective appetite-regulating substances satietin and satietin-D had up to the present been prepared from human or animal blood serum or plasma by ultrafiltration, gel chromatography, treatment with trichloroacetic acid, affinity chromatography and optionally treatment with a proteolytic enzyme. According to the invention, satietin and satietin-D are obtained in a purified form by using immunoabsorption.
IN
Paul Horan, Bruce D Jensen, Sue Slezak assigned to SmithKline Beckman Corporation Methods for determining growth rate of cells growing in vivo and in vitro. Cells are labelled with cyanine dyes and changes in plasma membrane cyanine dye levels are used to determine growth rate. Cell growth rate determinations are utilized to monitor transplanted bone marrow cell engraftment and post-surgical corneal epirhea[ cell growth. The invented methods also are used to determine tumor cell sensitivity to cancer therapeutic agents, yeast sensitivity to antifungal agents, and bacteria sensitivity to antibacterial agents. 4859585 IN-VITRO METHODS FOR IDENTIFYING COMPOSITIONS WHICH ARE AGONISTS AND ANTAGONISTS OF ESTROGENS Carlos Sonnenschein, Ana Soto assigned to Trustees of Tufts College The present invention provides two general protocols by which a substance may be identified and characterized as an estrogen agonist and/or an estrogen antagonist. The protocols are invitro methods which utilize estrogen dependent cells in culture and a medium comprising an inhibitor endogenous to the sera of adult humans which is able to prevent the proliferation of cultured cells in-vitro. The methodology is rapid, reproducible, accurate and provides the major advantage of being able to screen large numbers of substances for their primary or secondary side effects in a large variety of medical and environmental applications. 4859586 DEVICE FOR CULTIVATING BACTERIA Eliahu Eisenberg, Reuven Padova, Avner Shenfeld, Tsvi Hirshfeld, Jacob Stein, Tel Aviv, Israel
409
There is provided a hermetically closed culture vessel in which miroorganisms are cultivated simultaneously or consecutively on a liquid cuiture medium and on one or more solid culture media. The culture vessel is transparent, and contains in its lower part a liquid culture medium. From the cap, closing the vessel, there extends downwardly an elongated support for the solid culture medium or media, which is essentiaUy parallel with the central axis of the vessel, which terminates above the liquid culture medium. The growth on the solid culture medium is conveniently inspected from the side of the culture vessel.
4859603 PERSONAL
DIAGNOSTIC
KIT
Charles M Dole, Gary L Webster assigned to Dole Associates Inc A personal, disposable hand held diagnostic kit, having a specimen support member such as a membrane, wafer on the like including a plurality of liquid receptacles with means for applying the liquids to said support member in sequence for relatively immediate observation of reaction and ultimate diagnosis.
4859766 PURIFIED
APPETITE-
REGULATING SUBSTANCES OF BIOLOGICAL ORGIN, THEIR ANTIBODIF.~ IMMUNOCOMPLEXES OF THE APPETITE-REGULATING SUBSTANCES FORMED WITH THE ANTIBODIES AND PROCESSES FOR PREPARING SAM[E Jozse Knoll, Sando Harmath, Janos Nagy, Berta Knoll, Budapest, Hungary assigned to Richter G-edeon Vegyeszeti The selective appetite-regulating substances satietin and satietin-D had up to the present been prepared from human or animal blood serum or plasma by ultrafiltration, gel chromatography, treatment with trichloroacetic acid, affinity chromatography and optionally treatment with a proteolytic enzyme. According to the invention, satietin and satietin-D are obtained in a purified form by using immunoabsorption.