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4864019 Antibodies to inhibin and conjugates produced therefrom
PATENT ABSTRACTS purify biological materials of interest containing the receptor structure. 4863867 GAS PERMEABLE PACK
BIO-TEST
James Joyce, Armineh Khachatoorian assigned to Becton Dickinson and Company A disposable sterilization bio-test pack for determining the efficacy of sterilizing equipment comprises a porous base pad having a cavity for receiving the sterilization indicator. Porous pads are disposed on opposite sides ofthe base pad to retain the sterilization indicator within the cavity. The porous medium creates a restriction to the steam's access to the sterilization indicator in order to establish the efficacy of the sterilization equipment, 4863868 APPARATUS FOR DETECTING THE PRESENCE OF MICRO ORGANISM IN LIQUID
METHOD OF THIOACYLATION PEPTIDE SEQUENCING WITH ALCOHOLYSIS OF THIAZOLINONES Mark L Stolowitz, Beth Paape, Anne E DeVaux assigned to Bio-Affinity Systems Inc A sequencing method which exploits the thioacylation degradation of polypeptides and proteins is disclosed. The process involves reaction of the N-terminal amino acid of a polypeptide with an excess of a thioacylating reagent. After sufficient time to insure quantitative coupling and removal of excess reagent, the Nthioacyl polypeptide is subjected to cleavage by acid which affords a 2-substituted-5(4H) thiazolinone of the N-terminal amino acid. Subsequent addition of a large excess of an aliphatic primary or secondary alcohol, either directly to the cleavage acid or after its removal, yields the corresponding N-thioacyl amino acid ester, a stable compound suitable for chromatographic separation and subsequent detection by contemporary methods of high pressure liquid chromatography.
John E F Holley, Near Westerham, United Kingdom assigned to Prolitec Aktiengesellschaft An analyzer for detecting and measuring microorganisms in liquids comprising a probe with an orifice adapted to have liquid containing particles passed through it, and a means for passing cleansing liquid over the orifice is disclosed.
4864019 ANTIBODIES
TO INHIBIN AND
CONJUGATES PRODUCED THEREFROM Wylie W Vale, Jean E F Rivier, Joachi Spicss assigned to The Salk Institute for Biological Studies
4863869 ANTI-HUMAN IGM IMMUNOADSORBENT AND PROCESS FOR PRODUCING IMMUNOADSORBENT
417 4863870
SAID
Joseph P Balint assigned to Imre Cororation An immunoadsorbent material for removing IgM and IgM-eomplexes from biological fluids is prepared by covalently binding anti-IgM antibodies to a solid-phase silica matrix. It has been found that reacting hydroxy-derivatized silica in the presence of cyanogen bromide with the antiIgM antibodies provides a particularly stable, high-capacity immunoadsorbent. The immunoadsorbent material may be employed in a column for therapeutic treatment of various disorders, such as primary biliary cirrhosis,
A 32,000-Dalton protein with inhibin activity is isolated from porcine follicular fluid which is composed of two chains having molecular weights of about 18,000 and about 14,000 Daltons, which are bound together by disulfide bonding. Microsequencing revealed the NH2terminal portion of the 18 kD chain to be SerThr-Ala-Pro-Leu-Pro Trp-Pro-Trp-Ser-ProAla-Ala Leu-Arg-Leu-Leu-GIn-Ar g- Pro-ProGlu-Glu-Pro-Ala-Val and of the 14 kD chain to be Gly-Leu-Glu-Cys with the next 21 residues believed to be Asp-Gly-Ser-His-Asn-Leu-Asp Ser-Arg-GIn-Gln-Phe-Phe-Ile Asp-Phe-ArgLeu-ll e-G y-Trp. This 32 kD protein specifically inhibits basal secretion of FSH, but not of LH. Antibodies raised against a synthetic replicate of the N-terminal six residues of the 18 kD chain are effective to reduce the activity of highly
4 la
PATENT ABSTRACTS
purified 32 kD inhibin and may be administered to mammalian animals for the purpose of neutralizing endogenous inhibin and thereby increasing gonadotropin secretion. The antibodies are also useful in immunoassays to determine inhibin levels in mammalian fluids. To generate the antibodies, short chain peptides are synthesized which mimic portions of natural mammalian inhibin protein chains, the synthetic peptides are conjugated to large carrier molecules and the conjugates are used as inoculum to induce a mammalian immune system to produce inhibin-reactive antibodies.
An antibody-based bioassay for enzymeinducing chemicals is disclosed. The percent induction of an enzyme or class of related enzymes in living cells by an enzyme-inducing chemical in a sample is determined before and after the addition to the sample of an antibody to the chemical. The concentration of the chemical before and after the addition of the antibody is determined by comparison to the percent induction by known concentrations of the chemical. This permits the determination of the concentration of the chemical in the sample.
4865973 4865969 PRODUCTION OF MICROORGANISMS Jean Amen, Miche Cabau Versailles, France assigned to Eurozyme S A This invention relates to the production of the microorganisms. The operation is conducted in two stages. The first stage is conducted in the absence of ultrafiltration while the pH of the growth medium is maintained within the range of 6-7 by the addition of a neutralizing agent, Nutrient substratum and dilution water are added during the first stage to maintain the growth rate at a constant level, and the volume of growth medium is permitted to increase. When the amount of growth inhibiting agent produced in the fermentation reaches a predetermined maximum level, the second stage is initiated and the growth rate is maintained in a range of 0.10 to 0.50/hr, which is less than the growth rate in the first stage. By increasing the amount of dilution water the concentration of the growth inhibiting agent is maintained so as to achieve the desired moderate growth rate. The growth inhibiting agent is also continuously removed from the growth medium duringthe second stage and the volume of the growth medium is maintained substantially constant. The invention may be applied to the production of lactic bacteria, in particular for example in the cheese and wine making industries,
PROCESS FOR EXTRACTIVE FERMENTATION Fin Kollerup, Andrew J Daugulis, Kingston, Canada assigned to Queen's University at Kingston A process for producing a variety of chemical products, e.g., ethanol, by fermentation in which the product is removed from the fermentation medium as it is formed by liquid-liquid extraction using an extractant for the product which is immiscible with water. The extractant employed is chosen from the following groups: (A) double bond unsaturated aliphatic alcohols having 12 or more carbon atoms; (B) saturated branched chain aliphatic alcohols having 14 or more carbon atoms or mixtures thereof; (C) double bond unsaturated aliphatic acids having 12 or more carbon atoms; (D) aliphatic and aromatic mono, di- or tri-esters having 12 or more carbon atoms, other than dibutyl phthalate; (E) aliphatic noncyclic ketones and aliphatic aldehydes having 12 or more carbon atoms; and (F) mixtures of extractants from groups (A) to (E) above or mixtures of at least one of the above extractants and at least one other extractant. These extractants are non-toxic to microorganisms used for fermentation, have little tendency to form emulsions and are otherwise suited for the process. The liquid-liquid extraction may thus be carried out in situ in the fermentor, preferably in a continuous stirred tank fermentor.
4865972
4865980
ANTIBODY-BASED BIOASSAY FOR ENZYME-INDUCING CHEMICALS
MONOCLONAL ANTIBODIES F O R D N A - R N A HYBRID COMPLEXES AND THEIR USES
Kenneth W Hunter assigned to Westinghouse Electric Corp
W Dorse Stuart, Mark B Frank assigned to University of Hawaii
BIO-TEST
James Joyce, Armineh Khachatoorian assigned to Becton Dickinson and Company A disposable sterilization bio-test pack for determining the efficacy of sterilizing equipment comprises a porous base pad having a cavity for receiving the sterilization indicator. Porous pads are disposed on opposite sides ofthe base pad to retain the sterilization indicator within the cavity. The porous medium creates a restriction to the steam's access to the sterilization indicator in order to establish the efficacy of the sterilization equipment, 4863868 APPARATUS FOR DETECTING THE PRESENCE OF MICRO ORGANISM IN LIQUID
METHOD OF THIOACYLATION PEPTIDE SEQUENCING WITH ALCOHOLYSIS OF THIAZOLINONES Mark L Stolowitz, Beth Paape, Anne E DeVaux assigned to Bio-Affinity Systems Inc A sequencing method which exploits the thioacylation degradation of polypeptides and proteins is disclosed. The process involves reaction of the N-terminal amino acid of a polypeptide with an excess of a thioacylating reagent. After sufficient time to insure quantitative coupling and removal of excess reagent, the Nthioacyl polypeptide is subjected to cleavage by acid which affords a 2-substituted-5(4H) thiazolinone of the N-terminal amino acid. Subsequent addition of a large excess of an aliphatic primary or secondary alcohol, either directly to the cleavage acid or after its removal, yields the corresponding N-thioacyl amino acid ester, a stable compound suitable for chromatographic separation and subsequent detection by contemporary methods of high pressure liquid chromatography.
John E F Holley, Near Westerham, United Kingdom assigned to Prolitec Aktiengesellschaft An analyzer for detecting and measuring microorganisms in liquids comprising a probe with an orifice adapted to have liquid containing particles passed through it, and a means for passing cleansing liquid over the orifice is disclosed.
4864019 ANTIBODIES
TO INHIBIN AND
CONJUGATES PRODUCED THEREFROM Wylie W Vale, Jean E F Rivier, Joachi Spicss assigned to The Salk Institute for Biological Studies
4863869 ANTI-HUMAN IGM IMMUNOADSORBENT AND PROCESS FOR PRODUCING IMMUNOADSORBENT
417 4863870
SAID
Joseph P Balint assigned to Imre Cororation An immunoadsorbent material for removing IgM and IgM-eomplexes from biological fluids is prepared by covalently binding anti-IgM antibodies to a solid-phase silica matrix. It has been found that reacting hydroxy-derivatized silica in the presence of cyanogen bromide with the antiIgM antibodies provides a particularly stable, high-capacity immunoadsorbent. The immunoadsorbent material may be employed in a column for therapeutic treatment of various disorders, such as primary biliary cirrhosis,
A 32,000-Dalton protein with inhibin activity is isolated from porcine follicular fluid which is composed of two chains having molecular weights of about 18,000 and about 14,000 Daltons, which are bound together by disulfide bonding. Microsequencing revealed the NH2terminal portion of the 18 kD chain to be SerThr-Ala-Pro-Leu-Pro Trp-Pro-Trp-Ser-ProAla-Ala Leu-Arg-Leu-Leu-GIn-Ar g- Pro-ProGlu-Glu-Pro-Ala-Val and of the 14 kD chain to be Gly-Leu-Glu-Cys with the next 21 residues believed to be Asp-Gly-Ser-His-Asn-Leu-Asp Ser-Arg-GIn-Gln-Phe-Phe-Ile Asp-Phe-ArgLeu-ll e-G y-Trp. This 32 kD protein specifically inhibits basal secretion of FSH, but not of LH. Antibodies raised against a synthetic replicate of the N-terminal six residues of the 18 kD chain are effective to reduce the activity of highly
4 la
PATENT ABSTRACTS
purified 32 kD inhibin and may be administered to mammalian animals for the purpose of neutralizing endogenous inhibin and thereby increasing gonadotropin secretion. The antibodies are also useful in immunoassays to determine inhibin levels in mammalian fluids. To generate the antibodies, short chain peptides are synthesized which mimic portions of natural mammalian inhibin protein chains, the synthetic peptides are conjugated to large carrier molecules and the conjugates are used as inoculum to induce a mammalian immune system to produce inhibin-reactive antibodies.
An antibody-based bioassay for enzymeinducing chemicals is disclosed. The percent induction of an enzyme or class of related enzymes in living cells by an enzyme-inducing chemical in a sample is determined before and after the addition to the sample of an antibody to the chemical. The concentration of the chemical before and after the addition of the antibody is determined by comparison to the percent induction by known concentrations of the chemical. This permits the determination of the concentration of the chemical in the sample.
4865973 4865969 PRODUCTION OF MICROORGANISMS Jean Amen, Miche Cabau Versailles, France assigned to Eurozyme S A This invention relates to the production of the microorganisms. The operation is conducted in two stages. The first stage is conducted in the absence of ultrafiltration while the pH of the growth medium is maintained within the range of 6-7 by the addition of a neutralizing agent, Nutrient substratum and dilution water are added during the first stage to maintain the growth rate at a constant level, and the volume of growth medium is permitted to increase. When the amount of growth inhibiting agent produced in the fermentation reaches a predetermined maximum level, the second stage is initiated and the growth rate is maintained in a range of 0.10 to 0.50/hr, which is less than the growth rate in the first stage. By increasing the amount of dilution water the concentration of the growth inhibiting agent is maintained so as to achieve the desired moderate growth rate. The growth inhibiting agent is also continuously removed from the growth medium duringthe second stage and the volume of the growth medium is maintained substantially constant. The invention may be applied to the production of lactic bacteria, in particular for example in the cheese and wine making industries,
PROCESS FOR EXTRACTIVE FERMENTATION Fin Kollerup, Andrew J Daugulis, Kingston, Canada assigned to Queen's University at Kingston A process for producing a variety of chemical products, e.g., ethanol, by fermentation in which the product is removed from the fermentation medium as it is formed by liquid-liquid extraction using an extractant for the product which is immiscible with water. The extractant employed is chosen from the following groups: (A) double bond unsaturated aliphatic alcohols having 12 or more carbon atoms; (B) saturated branched chain aliphatic alcohols having 14 or more carbon atoms or mixtures thereof; (C) double bond unsaturated aliphatic acids having 12 or more carbon atoms; (D) aliphatic and aromatic mono, di- or tri-esters having 12 or more carbon atoms, other than dibutyl phthalate; (E) aliphatic noncyclic ketones and aliphatic aldehydes having 12 or more carbon atoms; and (F) mixtures of extractants from groups (A) to (E) above or mixtures of at least one of the above extractants and at least one other extractant. These extractants are non-toxic to microorganisms used for fermentation, have little tendency to form emulsions and are otherwise suited for the process. The liquid-liquid extraction may thus be carried out in situ in the fermentor, preferably in a continuous stirred tank fermentor.
4865972
4865980
ANTIBODY-BASED BIOASSAY FOR ENZYME-INDUCING CHEMICALS
MONOCLONAL ANTIBODIES F O R D N A - R N A HYBRID COMPLEXES AND THEIR USES
Kenneth W Hunter assigned to Westinghouse Electric Corp
W Dorse Stuart, Mark B Frank assigned to University of Hawaii