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5202235 Enzymatic method for the synthesis and separation of peptides
195
PATENT ABSTRACTS A carrier for a biologically active component for immunoassay or enzymatic reaction, which comprises: a) a thermoplastic resin bead having an average diameter of from 0.05 to 20 ram, b) from I to 25% by weight, based on the bead, of a magnetically responsive powder bonded to the bead, and c) a polymer coated thereon in a thickness of from 2 to 30 mum, said polymer having a number average molecular weight of from 200 to 10,000 and having functional groups capable of binding, or being activated to bind, the biologically active component.
5200334 SOL-GEL
ENCAPSULATED ENZYME
Bruce S Dunn, Joan Valentine, Jeffrey I Zink, Lisa Ellerby, Fumito Nishida, Clinton Nishida, Stacey Yamanaka, Tokyo, assigned to The Regents of the University of California An active biological material encapsulated in a glass is formed using a sol-gel process. A metal alkoxide is mixed with water and exposed to ultrasonic energy at a pH< or = 2 to form a single phase solution which is then buffered to a pH between about 5 and 7. The buffered solution is then mixed with the active biological material and the resultant gel is aged and dried. The dried product is a transparent porous glass with substantiaUy all of the added active biological material encapsulated therein, the biological material retaining a high level of activity.
5200339 PROTEASES CAUSING ABNORMAL DEGRADATION OF AMYLOID BETA-PROTEIN PRECURSOR Carmela R Abraham A proteolytic factor is capable of cleaving betaprotein precursor at a site near the beta-protein N-terminus. Also, a method for treating Alzheimer's disease in a patient includes steps of reducing beta-protein precursor proteolysis outside the beta-protein domain at a site near the beta-protein N-terminus. Also, a method for purifying an enzyme from a sample includes steps of incubating the sample with a labelled substrate of the enzyme or with a labelled fragment of a substrate to which the enzyme binds,
treating the sample with a crosslinking agent to crosslink any enzyme-substrate complexes in the sample, and recovering labelled complexes. Also, a method for diagnosis in a subject of a disease characterized by accumulation of amyloid includes determining the level, in a sample of tissue or body fluid from the subject, of an AD proteolytic factor. Also, a method for screening for an agent useful in treatment of a disease characterized by accumulation of amyloid includes steps of incubating an AD protease with a peptide having an amino acid sequence corresponding to the sequence spanning the beta-protein N-terminus in the presence of a candidate agent, and determining the degradation of the peptide.
5201370 ENZYME BREAKER FOR GALACTOMANNAN BASED FRACTURING FLUID Robert M Tjon-Joe-Pin assigned to BJ Services Company A method of fracturing a subterranean formation in a well bore is shown in which a gellable fracturing fluid is first formed by blending together an aqueous fluid, a hydratable polymer, a suitable cross-linking agent for cross-linking the hydratable polymer to form a polymer gel and an enzyme breaker. The cross-linked polymer gel is pumped into the well bore under sufficient pressure to fracture the surrounding formation. The enzyme breaker is allowed to degrade the cross-linked polymer with time to reduce the viscosity of the fluid so that the fluid can be pumped from the formation back to the well surface. The particular enzyme breaker utilized has an activity in the range from about 2.0 to 11.0 and is effective to attack only specific linkages in the cross-linked polymer gel.
5202235 ENZYMATIC METHOD FOR THE SYNTHESIS AND SEPARATION OF PEPTIDES Guillermo A Iacobucci assigned to The CocaCola Company The present invention provides a method for the enzymatic synthesis ofpeptides accomplished by shifting the chemical equilibrium that exists in a reaction mixture between charged or ionized
196
PATENT ABSTRACTS
reacting amino acids and uncharged or nonionized peptide product in the presence of a proteolytic enzyme such as thermolysin. The equilibrium is shifted by diffusion of the uncharged peptide product across an ion-rejection membrane which removes the uncharged peptide from the reaction mixture and preferably the diffused uncharged peptide is quickly converted to a charged species that cannot back-diffuse into the reaction mixture so that the uncharged peptide is effectively pulled across the membrane.
5202416 PROTEASE ABSORBENT FOR ISOLATING AND PURIFYING PROTEASES, A PROCESS FOR THE PREPARATION THEREFOR, AND THE METHOD FOR PURIFYING A PROTEASE Werne Stuber, ques Eric P Pcir/a/, Lahntal, Federal Republic Of Germany assigned to Behringwerke Aktiengesellschaft Peptide derivatives of the Formula I See Patent for Tabular Presentation PS in which Y is HN(CH2)n-CO with n = 1 to 8 or a bond, X is CN, CH2OH or CHO and R is a support or a hydrogen atom, a process for the preparation thereof, and the use thereof for affinity chromatography, especially for isolating and purifying proteases, are described.
set between 200 and 500 mV by the addition to an acid aqueous solution, which contains lignitic raw materials, of oxidizing agents and/or reducing agents and/or salts and/or phenolic compounds. The lignin degrading reaction and its attendant simultaneous bleaching effect is initiated by the addition of enzymes, microorganisms, animal or plant cells. Continuous stirring allows the reaction to be maintained for several hours at a value that fluctuates about a constant redox potential value, and a constant temperature.
5204245 EXTRACTION AND OTHER
OF THYMIDINE NUCLEOSIDES
James Doncheck, James R Millis, Paul E Swanson assigned to E I du Pont de Nemours and Company An enzymatic process for extracting a nucleoside, such as thymidine, from a biomass without substantial thymine production.
5204251 PROCESS OF ENZYMATIC 1NTERESTERIFICATION MAINTAINING A WATER CONTENT OF 30-300 PPM USING RHIZOPUS
5203964 PROCESS FOR PRODUCING CELLULOSE FROM LIGNIN CONTAINING RAW MATERIALS USING AN ENZYME OR MICROORGANISM WHILE MONITORING AND MAINTAINING THE REDOX POTENTIAL Hans-Peter Call, D 5132 Ubach Palenberg, Federal Republic Of Germany A process and an apparatus capable of removing and/or transforming lignin or its degradation products present in material containing lignocellulose. In the present process, a redox potential is
Susumu Kyotani, Isao Tsuiimura, Hideki Fukuda, D 5132Ubach~Palenberg, assigned to Kanegafuchi Kagaku Kogyo & Kabushiki Kaisha A process of an enzymatic interesterification in a low water content condition, which comprises subjecting a reaction liquid containing (a) a fat or oil and (b) a member selected from the group consisting of(i) another fat or oil, (ii) a fatty acid ester with a lower alcohol and (iii) a fatty acid to the action of a lipase in the form of dried Rhizopus cells while maintaining the water concentration between 5 and 1000 ppm in the reaction liquid during the reaction. In accordance with the process of the present invention, an interesterification of fat or oil can be carried out efficiently in a high yield.
PATENT ABSTRACTS A carrier for a biologically active component for immunoassay or enzymatic reaction, which comprises: a) a thermoplastic resin bead having an average diameter of from 0.05 to 20 ram, b) from I to 25% by weight, based on the bead, of a magnetically responsive powder bonded to the bead, and c) a polymer coated thereon in a thickness of from 2 to 30 mum, said polymer having a number average molecular weight of from 200 to 10,000 and having functional groups capable of binding, or being activated to bind, the biologically active component.
5200334 SOL-GEL
ENCAPSULATED ENZYME
Bruce S Dunn, Joan Valentine, Jeffrey I Zink, Lisa Ellerby, Fumito Nishida, Clinton Nishida, Stacey Yamanaka, Tokyo, assigned to The Regents of the University of California An active biological material encapsulated in a glass is formed using a sol-gel process. A metal alkoxide is mixed with water and exposed to ultrasonic energy at a pH< or = 2 to form a single phase solution which is then buffered to a pH between about 5 and 7. The buffered solution is then mixed with the active biological material and the resultant gel is aged and dried. The dried product is a transparent porous glass with substantiaUy all of the added active biological material encapsulated therein, the biological material retaining a high level of activity.
5200339 PROTEASES CAUSING ABNORMAL DEGRADATION OF AMYLOID BETA-PROTEIN PRECURSOR Carmela R Abraham A proteolytic factor is capable of cleaving betaprotein precursor at a site near the beta-protein N-terminus. Also, a method for treating Alzheimer's disease in a patient includes steps of reducing beta-protein precursor proteolysis outside the beta-protein domain at a site near the beta-protein N-terminus. Also, a method for purifying an enzyme from a sample includes steps of incubating the sample with a labelled substrate of the enzyme or with a labelled fragment of a substrate to which the enzyme binds,
treating the sample with a crosslinking agent to crosslink any enzyme-substrate complexes in the sample, and recovering labelled complexes. Also, a method for diagnosis in a subject of a disease characterized by accumulation of amyloid includes determining the level, in a sample of tissue or body fluid from the subject, of an AD proteolytic factor. Also, a method for screening for an agent useful in treatment of a disease characterized by accumulation of amyloid includes steps of incubating an AD protease with a peptide having an amino acid sequence corresponding to the sequence spanning the beta-protein N-terminus in the presence of a candidate agent, and determining the degradation of the peptide.
5201370 ENZYME BREAKER FOR GALACTOMANNAN BASED FRACTURING FLUID Robert M Tjon-Joe-Pin assigned to BJ Services Company A method of fracturing a subterranean formation in a well bore is shown in which a gellable fracturing fluid is first formed by blending together an aqueous fluid, a hydratable polymer, a suitable cross-linking agent for cross-linking the hydratable polymer to form a polymer gel and an enzyme breaker. The cross-linked polymer gel is pumped into the well bore under sufficient pressure to fracture the surrounding formation. The enzyme breaker is allowed to degrade the cross-linked polymer with time to reduce the viscosity of the fluid so that the fluid can be pumped from the formation back to the well surface. The particular enzyme breaker utilized has an activity in the range from about 2.0 to 11.0 and is effective to attack only specific linkages in the cross-linked polymer gel.
5202235 ENZYMATIC METHOD FOR THE SYNTHESIS AND SEPARATION OF PEPTIDES Guillermo A Iacobucci assigned to The CocaCola Company The present invention provides a method for the enzymatic synthesis ofpeptides accomplished by shifting the chemical equilibrium that exists in a reaction mixture between charged or ionized
196
PATENT ABSTRACTS
reacting amino acids and uncharged or nonionized peptide product in the presence of a proteolytic enzyme such as thermolysin. The equilibrium is shifted by diffusion of the uncharged peptide product across an ion-rejection membrane which removes the uncharged peptide from the reaction mixture and preferably the diffused uncharged peptide is quickly converted to a charged species that cannot back-diffuse into the reaction mixture so that the uncharged peptide is effectively pulled across the membrane.
5202416 PROTEASE ABSORBENT FOR ISOLATING AND PURIFYING PROTEASES, A PROCESS FOR THE PREPARATION THEREFOR, AND THE METHOD FOR PURIFYING A PROTEASE Werne Stuber, ques Eric P Pcir/a/, Lahntal, Federal Republic Of Germany assigned to Behringwerke Aktiengesellschaft Peptide derivatives of the Formula I See Patent for Tabular Presentation PS in which Y is HN(CH2)n-CO with n = 1 to 8 or a bond, X is CN, CH2OH or CHO and R is a support or a hydrogen atom, a process for the preparation thereof, and the use thereof for affinity chromatography, especially for isolating and purifying proteases, are described.
set between 200 and 500 mV by the addition to an acid aqueous solution, which contains lignitic raw materials, of oxidizing agents and/or reducing agents and/or salts and/or phenolic compounds. The lignin degrading reaction and its attendant simultaneous bleaching effect is initiated by the addition of enzymes, microorganisms, animal or plant cells. Continuous stirring allows the reaction to be maintained for several hours at a value that fluctuates about a constant redox potential value, and a constant temperature.
5204245 EXTRACTION AND OTHER
OF THYMIDINE NUCLEOSIDES
James Doncheck, James R Millis, Paul E Swanson assigned to E I du Pont de Nemours and Company An enzymatic process for extracting a nucleoside, such as thymidine, from a biomass without substantial thymine production.
5204251 PROCESS OF ENZYMATIC 1NTERESTERIFICATION MAINTAINING A WATER CONTENT OF 30-300 PPM USING RHIZOPUS
5203964 PROCESS FOR PRODUCING CELLULOSE FROM LIGNIN CONTAINING RAW MATERIALS USING AN ENZYME OR MICROORGANISM WHILE MONITORING AND MAINTAINING THE REDOX POTENTIAL Hans-Peter Call, D 5132 Ubach Palenberg, Federal Republic Of Germany A process and an apparatus capable of removing and/or transforming lignin or its degradation products present in material containing lignocellulose. In the present process, a redox potential is
Susumu Kyotani, Isao Tsuiimura, Hideki Fukuda, D 5132Ubach~Palenberg, assigned to Kanegafuchi Kagaku Kogyo & Kabushiki Kaisha A process of an enzymatic interesterification in a low water content condition, which comprises subjecting a reaction liquid containing (a) a fat or oil and (b) a member selected from the group consisting of(i) another fat or oil, (ii) a fatty acid ester with a lower alcohol and (iii) a fatty acid to the action of a lipase in the form of dried Rhizopus cells while maintaining the water concentration between 5 and 1000 ppm in the reaction liquid during the reaction. In accordance with the process of the present invention, an interesterification of fat or oil can be carried out efficiently in a high yield.