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5436143 Method for enzymatic synthesis of oligonucleotides
PATENT ABSTRACTS
The invention describes the preparation and use of fluorescent stains for nucleic acids derived from unsymmetrical cyanine dyes. In particular, the invention describes unsymmetrical cyanine dyes having a saturated or unsaturated cyclic substituent. The dyes of the invention possess superior fluorescent characteristics when complexed with nucleic acids, and have utility in any application which requires detection of nucleic acids. The presence of the cyclic substituent results in improved permeability in a wide range of living cells, resulting in improved detection of intracellular nucleic acids.
5436136 REPRESSIBLE YEAST PROMOTERS Hinnen Albert; Meyhack Bernd Basel, SWITZERLAND assigned to Ciba-Geigy Corporation Novel upstream activation sites of the yeast PHO5 gene are used to produce inducible yeast hybrid promoters. The yeast hybrid promoters can be used to control transcription of a polypeptide coding region foreign to yeast in a yeast expression vector.
5436143 METHOD FOR ENZYMATIC SYNTHESIS OF OLIGONUCLEOTIDES Hyman Edward D River Ridge, LA, UNITED STATES Enzymatic synthesis of oligonucleotides may be performed in a single vessel without intermediate purification, by the steps of: (a) combining a nucleotide primer sequence and a blocked nucleotide in the presence of a chain extending enzyme whereby a reaction mixture is formed containing the blocked nucleotide coupled to the nucleotide primer sequence at its 3' end; (b) inactivating the chain extending enzyme; (c) removing the blocking group from the primer-blocked nucleotide to form a primer-nucleotide product; and converting any unreacted blocked nucleotide to an unreactive
503
form which is substantially less active as a substrate for the chain extending enzyme than the blocked nucleotide.
5436144 PROCESS FOR PERFORMING PCR IN MAMMALIAN CELLS Stewart Carleton; Timm Earl A Orchard Park, NY, UNITED STATES assigned to Health Research lnc A process is provided for performing polymerase chain reactions inside of intact cells. Measurement of genetic parameters and observation of genetic properties while maintaining the integrity of the DNA or RNA in a cell is accomplished by passing a suspension of cells through a flow cytometer wherein the properties and parameters can be measured on a cell by cell basis.
5436147 HETEROBIFUNCTIONAL CROSSLINKED AGENTS FOR IMMOBILIZING MOLECULES ON PLASTIC SUBSTRATES Pegg Randall K; Saunders Mary S Amelia Island, FL, UNITED STATES assigned to Nucleic Assays Corporation Heterobifunctional crosslinking agents are synthesized that covalently link molecules such as enzymes, ceils, proteins and nucleic acids to a plastic snbstrate. The agents contain a central ring structure having a hydrophobic hydrocarbon chain that binds to a plastic substrate and distal to the hydrophobic chain one or more hydrophilic chainsterminating in a reactive group that covalently binds the molecule. Immobilized molecules are useful in diagnostic assays or bioreactors. Preferred heterobifunctional crosslinking agents have the following structures: (*See Patent for Chemical Structure*) NOOONOONOOONOO and NHOOONOO NHOOONOO.
The invention describes the preparation and use of fluorescent stains for nucleic acids derived from unsymmetrical cyanine dyes. In particular, the invention describes unsymmetrical cyanine dyes having a saturated or unsaturated cyclic substituent. The dyes of the invention possess superior fluorescent characteristics when complexed with nucleic acids, and have utility in any application which requires detection of nucleic acids. The presence of the cyclic substituent results in improved permeability in a wide range of living cells, resulting in improved detection of intracellular nucleic acids.
5436136 REPRESSIBLE YEAST PROMOTERS Hinnen Albert; Meyhack Bernd Basel, SWITZERLAND assigned to Ciba-Geigy Corporation Novel upstream activation sites of the yeast PHO5 gene are used to produce inducible yeast hybrid promoters. The yeast hybrid promoters can be used to control transcription of a polypeptide coding region foreign to yeast in a yeast expression vector.
5436143 METHOD FOR ENZYMATIC SYNTHESIS OF OLIGONUCLEOTIDES Hyman Edward D River Ridge, LA, UNITED STATES Enzymatic synthesis of oligonucleotides may be performed in a single vessel without intermediate purification, by the steps of: (a) combining a nucleotide primer sequence and a blocked nucleotide in the presence of a chain extending enzyme whereby a reaction mixture is formed containing the blocked nucleotide coupled to the nucleotide primer sequence at its 3' end; (b) inactivating the chain extending enzyme; (c) removing the blocking group from the primer-blocked nucleotide to form a primer-nucleotide product; and converting any unreacted blocked nucleotide to an unreactive
503
form which is substantially less active as a substrate for the chain extending enzyme than the blocked nucleotide.
5436144 PROCESS FOR PERFORMING PCR IN MAMMALIAN CELLS Stewart Carleton; Timm Earl A Orchard Park, NY, UNITED STATES assigned to Health Research lnc A process is provided for performing polymerase chain reactions inside of intact cells. Measurement of genetic parameters and observation of genetic properties while maintaining the integrity of the DNA or RNA in a cell is accomplished by passing a suspension of cells through a flow cytometer wherein the properties and parameters can be measured on a cell by cell basis.
5436147 HETEROBIFUNCTIONAL CROSSLINKED AGENTS FOR IMMOBILIZING MOLECULES ON PLASTIC SUBSTRATES Pegg Randall K; Saunders Mary S Amelia Island, FL, UNITED STATES assigned to Nucleic Assays Corporation Heterobifunctional crosslinking agents are synthesized that covalently link molecules such as enzymes, ceils, proteins and nucleic acids to a plastic snbstrate. The agents contain a central ring structure having a hydrophobic hydrocarbon chain that binds to a plastic substrate and distal to the hydrophobic chain one or more hydrophilic chainsterminating in a reactive group that covalently binds the molecule. Immobilized molecules are useful in diagnostic assays or bioreactors. Preferred heterobifunctional crosslinking agents have the following structures: (*See Patent for Chemical Structure*) NOOONOONOOONOO and NHOOONOO NHOOONOO.