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5436143 Method for enzymatic synthesis of oligonucleotides
PATENT ABSTRACTS
5434074 CYTOMEGALOVIRUS PROTEINASE Gibson DWade;WelchAnthonyR Baitimore, MD, UNITED STATES A herpes virus proteinase has been found to be encoded by a member of a family of four nested genes in simian cytomegalovirus. Another member of the nested genes encodes the assembly protein precursor, which is a substrate for the proteinase. Homologous genes are found in other herpes viruses. Cleavage sites recognized by the proteinase are identified in cytomegalovirus and are found to be highly conserved in other herpes viruses. Substrates, inhibitors, assay kits, and methods of assaying are provided which rely on the proteinase and its activity.
5434248 PEPTIDYL DERIVATIVES AS INHIBITORS OF INTERLEUKIN-1 BETA CONVERTING ENZYME Chapman Kevin T; Bull Herb; MacCoss Malcolm; Thoruberry Nancy; Weidner Jeffrey; Mjalli Adnan M Scotch Plains, assigned to Merck & Co Inc Novel peptidyl derivatives of formula I are found to be potent inhibitors of interleukin-I beta converting enzyme (ICE). Compounds of formula I may be useful in the treatment of inflammatory or immune-based diseases of the lung and airways; central nervous system and surrounding membranes; the eyes and ears; joints, bones, and connective tissues; cardiovascular system including the pericardium; the gastrointestinal and urogenital systems; the skin and mucosal membranes. Compounds of formula I are also useful in treating the complications of infection (e.g., gram negative shock) and tumors in which IL 1 functions as an autocrine growth factor or as a mediator of cachexia. (*See Patent for Chemical Structure*)
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5435809 METHOD OF OBTAINING COLOR EFFECTS ON FABRIC OR GARMENTS USING FOAM CARRIERS AND CELLULASE ENZYMES Hoist L Thomas; Bownds Casey; Craven Richard; Waddell Robert Charlotte, NC, UNITED STATES assigned to Dexter Chemical Corp A method for the treatment of denim fabrics or garments to achieve a worn or distressed look wherein treatment comprises contacting a plurality of denim fabrics or garments with an effective amount of a foam composition which contains an effective amount of a cellulase enzyme. The enzyme is capable of altering the color depth of the dye in the fabric materials. The garments or fabrics are vignrously tumbled so that they repeatedly contact and rub against one another so that abrasive rubbing contact between the garments or fabrics is ensured, which imparts a worn or distressed look to the fabrics or garments.
5436143 METHOD FOR ENZYMATIC SYNTHESIS OF OLIGONUCLEOTIDES Hyman Edward D River Ridge, LA, UNITED STATES Enzymatic synthesis ofoligonucleotides may be performed in a single vessel without intermediate purification, by the steps of: (a) combining a nucleotide primer sequence and a blocked nucleotide in the presence of a chain extending enzyme whereby a reaction mixture is formed containing the blocked nucleotide coupled to the nucleotide primer sequence at its 3' end, (b) inactivating the chain extending enzyme, (c) removing the blocking group fi'om the primer-blocked nucleotide to form a primeronucleofide product; and converting any unreacted blocked nucleotide to an unreactive form which is substantially less active as a substrate for the chain extending enzyme than the blocked nucleotide.
5434074 CYTOMEGALOVIRUS PROTEINASE Gibson DWade;WelchAnthonyR Baitimore, MD, UNITED STATES A herpes virus proteinase has been found to be encoded by a member of a family of four nested genes in simian cytomegalovirus. Another member of the nested genes encodes the assembly protein precursor, which is a substrate for the proteinase. Homologous genes are found in other herpes viruses. Cleavage sites recognized by the proteinase are identified in cytomegalovirus and are found to be highly conserved in other herpes viruses. Substrates, inhibitors, assay kits, and methods of assaying are provided which rely on the proteinase and its activity.
5434248 PEPTIDYL DERIVATIVES AS INHIBITORS OF INTERLEUKIN-1 BETA CONVERTING ENZYME Chapman Kevin T; Bull Herb; MacCoss Malcolm; Thoruberry Nancy; Weidner Jeffrey; Mjalli Adnan M Scotch Plains, assigned to Merck & Co Inc Novel peptidyl derivatives of formula I are found to be potent inhibitors of interleukin-I beta converting enzyme (ICE). Compounds of formula I may be useful in the treatment of inflammatory or immune-based diseases of the lung and airways; central nervous system and surrounding membranes; the eyes and ears; joints, bones, and connective tissues; cardiovascular system including the pericardium; the gastrointestinal and urogenital systems; the skin and mucosal membranes. Compounds of formula I are also useful in treating the complications of infection (e.g., gram negative shock) and tumors in which IL 1 functions as an autocrine growth factor or as a mediator of cachexia. (*See Patent for Chemical Structure*)
5 61
5435809 METHOD OF OBTAINING COLOR EFFECTS ON FABRIC OR GARMENTS USING FOAM CARRIERS AND CELLULASE ENZYMES Hoist L Thomas; Bownds Casey; Craven Richard; Waddell Robert Charlotte, NC, UNITED STATES assigned to Dexter Chemical Corp A method for the treatment of denim fabrics or garments to achieve a worn or distressed look wherein treatment comprises contacting a plurality of denim fabrics or garments with an effective amount of a foam composition which contains an effective amount of a cellulase enzyme. The enzyme is capable of altering the color depth of the dye in the fabric materials. The garments or fabrics are vignrously tumbled so that they repeatedly contact and rub against one another so that abrasive rubbing contact between the garments or fabrics is ensured, which imparts a worn or distressed look to the fabrics or garments.
5436143 METHOD FOR ENZYMATIC SYNTHESIS OF OLIGONUCLEOTIDES Hyman Edward D River Ridge, LA, UNITED STATES Enzymatic synthesis ofoligonucleotides may be performed in a single vessel without intermediate purification, by the steps of: (a) combining a nucleotide primer sequence and a blocked nucleotide in the presence of a chain extending enzyme whereby a reaction mixture is formed containing the blocked nucleotide coupled to the nucleotide primer sequence at its 3' end, (b) inactivating the chain extending enzyme, (c) removing the blocking group fi'om the primer-blocked nucleotide to form a primeronucleofide product; and converting any unreacted blocked nucleotide to an unreactive form which is substantially less active as a substrate for the chain extending enzyme than the blocked nucleotide.