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A case of bullous pemphigoid which shows negative serum anti-BP180 antibody by commercial ELISA
Abstracts from the 41st Annual Meeting / Journal of Dermatological Science 86 (2017) e1–e95
However, the mechanisms underlying the improvement of these inflammatory skin diseases remain unknown. We investigated the anti-inflammatory effects of KI treatment by evaluating the histologic changes and the expression of genes encoding inflammatory cytokines in a mouse model of sodium dodecyl sulphate (SDS)induced inflammatory skin. Method: 5% of SDS + KI, which was dissolved in PBS, or PBS were applicate into the shaved back of BALB/C mice daily for 7 days. The back skin was removed on the two days after from final treatment. The component of skin thickness were measured from photographs obtained under a light microscope of hematoxylin & eosin-stained sections. The expressions of the genes encoding inflammatory cytokines (IL-1, TNF-aipha, IL-10, IL-17) in the skin samples were measured by real-time RT-PCR. Results: Marked reduction in the thickness of the epidermis was observed in the mice treated with SDS + KI treatment, competing with SDS treatment only. The gene expression levels of inflammatory cytokines were reduced by KI treatment. The variations of inflammatory cells are under investigation. http://dx.doi.org/10.1016/j.jdermsci.2017.02.047 P01-45[O1-27] The therapeutic potential and molecular mechanism of isoflavone extracts on anti-psoriatic activity Chi-Feng
Hung 1,∗ ,
Hsin-Ju
Li 2
1
School of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan 2 Department of Chemistry, Fu Jen Catholic University, New Taipei City, Taiwan Psoriasis is a common inflammatory disease, which may affect 1–3% population worldwide and generate increasing medical costs year by year. Typical psoriatic skin lesions are reddish thick scaly plaques which may occur on multiple skin sites of the whole body. Topical application of imiquimod (IMQ), a TLR7 agonist and potent immune activator, can induce and exacerbate psoriasis, a chronic inflammatory skin disorder. The past studies demonstrated that isoflavone extracts has an antioxidant effect, and may decrease inflammation and inflammatory pain. In the in vivo studies, we found that the topical application of isoflavone extracts before imiquimod treatment significantly decreased the value of transepidermal water loss (TEWL), erythema, the speed of blood flow, ear thickness and increased corneometer, and also attenuated epidermal hyperplasia and inflammatory cell infiltration. In the in vitro experiments, we found that isoflavone extracts can reduce IL-17A or TNF-alpha induced MAPK phosphorylation, NF-kappa B activation, CCL20 release, and inflammatory makers mRNA expression in normal human epidermal keratinocytes. These results indicate that isoflavone extracts has a great potential as an anti-psoriatic agent for the treatment of inflammatory skin diseases. http://dx.doi.org/10.1016/j.jdermsci.2017.02.048
e17
P01-46[O1-28] A case of bullous pemphigoid which shows negative serum anti-BP180 antibody by commercial ELISA Tie Duerna ∗ , Tokiko Yoshida, Yuko Chinuki, Eishin Morita The Department of Dermatology, Shimane University Faculty of Medicine, Izumo, Shimane, Japan A 93-year-old female patient, to whom diagnosis of bullous pemphigoid had been given 3 years ago, was referred to our department with new and tense blisters on her legs. Biopsies taken from her skin indicated subepidermal blisters with lymphocytes infiltration and without eosinophilic spongiosis. Direct immunofluorescence result indicated linear C3 deposition at the basement membrane zone. Indirect immunofluorescence using 1 M NaCl spilt human skin showed IgG binding to the epidermal side. While her anti-BP180 antibodies were negative by commercial ELISA, we confirmed her serum antibodies against BP180 by immunoblotting using normal epidermal extract. Whether this case is drug-induced pemphigoid or bullous pemphigoid with epitope spreading should be further considered. http://dx.doi.org/10.1016/j.jdermsci.2017.02.049 P01-47[O1-29] Lactobacillus pentosus GMNL-77 inhibits skin lesions in imiquimod-induced psoriasis-like mice Chieh-Shan Wu 1,∗ , Wen-Ho Chuo 2 , Yi-Hsing Chen 3 , Ya-Husan Chao 3 , Chi-Chen Lin 3,4 , Yi-Rong Li 4 , Wan-Hua Tsai 5 , Yu-Kuo Chen 6 1 Department of Dermatology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan 2 Department of Pharmacy, Tajen University, Pingtung, Taiwan 3 Institute of Biomedical Science, National Chung Hsing University, Taichung, Taiwan 4 Department of Medical Research and Education, Taichung Veterans General Hospital, Taichung, Taiwan 5 Research and Development Department, GenMont Biotech Incorportation, Taiwan 6 Deapartment of Food Science, National Pintung University of Science and Technology, Pintung, Taiwan Psoriasis, which is regarded as a T-cell mediated chronic inflammatory skin disease, is characterized by hyperproliferation and poor differentiation of epidermal keratinocytes. In this study, we aimed to determine the in vivo effect of a potentially probiotic strain, Lactobacillus pentosus (L. pentosus) GMNL-77, in imiquimod (IMQ)-induced epidermal hyperplasia and psoriasislike skin inflammation in BALB/c mice. Oral administration L. pentosus GMNL-77 significantly decreased erythematous scaling lesions. Real-time PCR showed that treatment with L. pentosus GMNL-77 significantly decreased the mRNA levels of proinflammatory cytokines, including TNF-alpha, IL-6, and the IL-23/IL-17A axis-associated cytokines, IL-23, IL-17A/F, and IL-22, in the skin of imiquimod-treated mice. In addition, we found that L. pentosus GMNL77 decreased the spleen weights of the IMQ-treated group and reduced the numbers of IL-17 and IL-22-producing CD4+ T cells in the spleen. In conclusion, the present study provides insight into
However, the mechanisms underlying the improvement of these inflammatory skin diseases remain unknown. We investigated the anti-inflammatory effects of KI treatment by evaluating the histologic changes and the expression of genes encoding inflammatory cytokines in a mouse model of sodium dodecyl sulphate (SDS)induced inflammatory skin. Method: 5% of SDS + KI, which was dissolved in PBS, or PBS were applicate into the shaved back of BALB/C mice daily for 7 days. The back skin was removed on the two days after from final treatment. The component of skin thickness were measured from photographs obtained under a light microscope of hematoxylin & eosin-stained sections. The expressions of the genes encoding inflammatory cytokines (IL-1, TNF-aipha, IL-10, IL-17) in the skin samples were measured by real-time RT-PCR. Results: Marked reduction in the thickness of the epidermis was observed in the mice treated with SDS + KI treatment, competing with SDS treatment only. The gene expression levels of inflammatory cytokines were reduced by KI treatment. The variations of inflammatory cells are under investigation. http://dx.doi.org/10.1016/j.jdermsci.2017.02.047 P01-45[O1-27] The therapeutic potential and molecular mechanism of isoflavone extracts on anti-psoriatic activity Chi-Feng
Hung 1,∗ ,
Hsin-Ju
Li 2
1
School of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan 2 Department of Chemistry, Fu Jen Catholic University, New Taipei City, Taiwan Psoriasis is a common inflammatory disease, which may affect 1–3% population worldwide and generate increasing medical costs year by year. Typical psoriatic skin lesions are reddish thick scaly plaques which may occur on multiple skin sites of the whole body. Topical application of imiquimod (IMQ), a TLR7 agonist and potent immune activator, can induce and exacerbate psoriasis, a chronic inflammatory skin disorder. The past studies demonstrated that isoflavone extracts has an antioxidant effect, and may decrease inflammation and inflammatory pain. In the in vivo studies, we found that the topical application of isoflavone extracts before imiquimod treatment significantly decreased the value of transepidermal water loss (TEWL), erythema, the speed of blood flow, ear thickness and increased corneometer, and also attenuated epidermal hyperplasia and inflammatory cell infiltration. In the in vitro experiments, we found that isoflavone extracts can reduce IL-17A or TNF-alpha induced MAPK phosphorylation, NF-kappa B activation, CCL20 release, and inflammatory makers mRNA expression in normal human epidermal keratinocytes. These results indicate that isoflavone extracts has a great potential as an anti-psoriatic agent for the treatment of inflammatory skin diseases. http://dx.doi.org/10.1016/j.jdermsci.2017.02.048
e17
P01-46[O1-28] A case of bullous pemphigoid which shows negative serum anti-BP180 antibody by commercial ELISA Tie Duerna ∗ , Tokiko Yoshida, Yuko Chinuki, Eishin Morita The Department of Dermatology, Shimane University Faculty of Medicine, Izumo, Shimane, Japan A 93-year-old female patient, to whom diagnosis of bullous pemphigoid had been given 3 years ago, was referred to our department with new and tense blisters on her legs. Biopsies taken from her skin indicated subepidermal blisters with lymphocytes infiltration and without eosinophilic spongiosis. Direct immunofluorescence result indicated linear C3 deposition at the basement membrane zone. Indirect immunofluorescence using 1 M NaCl spilt human skin showed IgG binding to the epidermal side. While her anti-BP180 antibodies were negative by commercial ELISA, we confirmed her serum antibodies against BP180 by immunoblotting using normal epidermal extract. Whether this case is drug-induced pemphigoid or bullous pemphigoid with epitope spreading should be further considered. http://dx.doi.org/10.1016/j.jdermsci.2017.02.049 P01-47[O1-29] Lactobacillus pentosus GMNL-77 inhibits skin lesions in imiquimod-induced psoriasis-like mice Chieh-Shan Wu 1,∗ , Wen-Ho Chuo 2 , Yi-Hsing Chen 3 , Ya-Husan Chao 3 , Chi-Chen Lin 3,4 , Yi-Rong Li 4 , Wan-Hua Tsai 5 , Yu-Kuo Chen 6 1 Department of Dermatology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan 2 Department of Pharmacy, Tajen University, Pingtung, Taiwan 3 Institute of Biomedical Science, National Chung Hsing University, Taichung, Taiwan 4 Department of Medical Research and Education, Taichung Veterans General Hospital, Taichung, Taiwan 5 Research and Development Department, GenMont Biotech Incorportation, Taiwan 6 Deapartment of Food Science, National Pintung University of Science and Technology, Pintung, Taiwan Psoriasis, which is regarded as a T-cell mediated chronic inflammatory skin disease, is characterized by hyperproliferation and poor differentiation of epidermal keratinocytes. In this study, we aimed to determine the in vivo effect of a potentially probiotic strain, Lactobacillus pentosus (L. pentosus) GMNL-77, in imiquimod (IMQ)-induced epidermal hyperplasia and psoriasislike skin inflammation in BALB/c mice. Oral administration L. pentosus GMNL-77 significantly decreased erythematous scaling lesions. Real-time PCR showed that treatment with L. pentosus GMNL-77 significantly decreased the mRNA levels of proinflammatory cytokines, including TNF-alpha, IL-6, and the IL-23/IL-17A axis-associated cytokines, IL-23, IL-17A/F, and IL-22, in the skin of imiquimod-treated mice. In addition, we found that L. pentosus GMNL77 decreased the spleen weights of the IMQ-treated group and reduced the numbers of IL-17 and IL-22-producing CD4+ T cells in the spleen. In conclusion, the present study provides insight into