Genome-wide analysis of Foxp3 target genes in human tongue squamous cell carcinoma cell

Oral Presentation Methods: The surgical approaches to tumor located in cranial base and endo–exocranial communication area in recent years were reviewed. In addition, respective advantages and disadvantages of these approaches were compared in the aspects of exposure of tumor, the protection of nerves and blood vessel postoperation complications and quality of life of patient. Results: Coronal incision with unilateral or bilateral frontal craniotomy is a suitable access to tumor at the base of anterior cranial fossa that involves the ethmoid sinus, sphenoid sinus, orbit and preglabellar area. Webber incision with maxillary swing approach provides the maximum direct exposure for anterior and middle skull base tumor that involves the base of orbit, sphenoid sinus and pterygopalatine fossa. The infratemporal fossa mastoid approach suits to lesions in the jugular foramen region. If the exocranial part of tumor is large and involves parapharyngeal, the mandibular swing approach should be considered, especially to benign tumor. The orbitozygomatic fronttemperal approach is suitable for the tumor extending prominently into the anterior and middle cranial base. Transoral trans-posterior wall pharynx is proper for tumor of clivus of the skull base and posterior pharyngeal wall. Conclusion: According to our study, choosing surgical approach as above can provide better tumor exposure protection of nerves and vessels improvement of quality of life and decrease the complications, especially when the tumor is large. http://dx.doi.org/10.1016/j.ijom.2015.08.666 Application of three transfer methods in computer-aided surgery technique in maxillary defect reconstruction J. Liang ∗ , X.F. Shan, J.W. Huang, K. Wang, Y. Zhang, L. Zhang, Z.G. Cai Department of Oral-Maxillofacial Surgery, School of Stomatology, Peking University, Beijing, China This study aims at proving the feasibility and comparing advantages and disadvantages of different CAS transfer methods in reconstruction of different classes of maxillary defects. 13 cases who received CAS assisted maxillary defect reconstruction surgery during March 2012–October 2014, were included in this study. Virtual surgical planning (VSP) conducted before surgery, transfer methods including: (1) individualized CAD/CAM implants, (2) 3D-printed models and (3) computer aided navigation surgery (CANS), were applied solely or in combination to convoy the VSP. CT scan were obtained in all patients pre- and post-operatively. The models of VSP and post-operation were introduced into Geomagic Qualify 12.0, deviation was calculated and shown in a color deviation map and a report. All patients achieved satisfying functional and aesthetical reconstruction outcome. All patients were able to take semi-fluid to normal food, with normal deglutition and speech function, no complication in eye movement and vision. Deviation between pre- and post-operative model surface under different tolerance is calculated, and indicates a trend that: (1) cases use CANS in reconstruction have smaller deviation than cases do not, (2) higher accuracy found in the cases using individualized CAD/CAM implants than those using 3D-printed model induced pre-formed Titanium Mesh. Conclusion: CAS is beneficial in maxillary defect reconstructions. CANS helps to control the safety, stability and accuracy of surgery, 3D printed model helps directly reflecting the VSP and implant shaping. Individualized CAD/CAM implant is beneficial in extensive defects reconstruction. Each transfer method has its advantages in convoying the VSP information,

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treatment suggestion is given based on the extent and anatomical characteristics of defect. http://dx.doi.org/10.1016/j.ijom.2015.08.667 Genome-wide analysis of Foxp3 target genes in human tongue squamous cell carcinoma cell Y. Liang ∗ , X. Lao, L. Liang, G. Liao Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, China Objective: The forkhead transcription factor Foxp3 is essential for differentiation and activation of Tregs. In the recent years, Foxp3 expressed in tumor cells (cancer cell-derived Foxp3) is of great interest, but its function and molecular mechanisms remain incompletely understood. In the present study, we aimed to illustrate the genome-wide target genes of cancer cell-derived Foxp3 in tongue squamous cell carcinoma (TSCC) cells to provide molecular basis for its functional research. Materials and methods: Dynamic nuclear translocation of Foxp3 in TSCC cells was detected by immunofluorescent staining. After Foxp3 down-regulation using siRNA, genome-wide analysis of Foxp3 target genes in TSCC cells were performed using a combination of ChIP-on-chip and whole-genome microarray assays. Results: Foxp3 in TSCC cells was transported into nucleus without exogenous stimuli. There were 3573 Foxp3-binding genes in TSCC cells. When Foxp3 was down-regulated, 599 genes were different-expressed in TSCC cells. Cross-referencing showed that 152 genes were identical in the ChIP-on-chip and expression profiling. About 12% Foxp3-binding genes in human TSCC cells were also Foxp3-biding genes in human Tregs. Bioinformatics analysis suggested that direct and indirect targets genes of Foxp3 in TSCC cells had distinct function compared from each other and from that in Tregs. Conclusions: Genome-wide analysis indicates that Foxp3 in TSCC cells has distinct biological functions comparing with that in Tregs. Cancer cell-derived Foxp3 directly regulate the transcription of genes that affect certain internal biological processes of TSCC cells, and indirectly influence the extracellular microenvironment. http://dx.doi.org/10.1016/j.ijom.2015.08.668 The effect of presurgical nasoalveolar molding (PNAM) on nasal symmetry in unilateral complete cleft lip/palate after primary cheiloplasty Z. Liang 1,∗ , J. Yao 1 , K.T.C. Philip 2 , C. Zheng 1 , J. Yang 1 1

The Second Hospital of Shenzhen, Shenzhen, Guangdong Province, China 2 Craniofacial Center, Chang Gung Memorial Hospital, Taipei, Taiwan Purpose: Nasal deformity is one of the most intractable problems in unilateral cleft lip/palate treatment. The purpose of this study was to assess the effect of presurgical nasoalveolar molding (PNAM) on the long-term nasal symmetry and shape in unilateral complete cleft lip/palate after the primary cheiloplasty. Methods: 84 complete unilateral cleft lip/palate patients in Chang Gung Craniofacial Center, Taiwan were divided into 2 groups: 42 patients in PNAM group and 42 patients in non PNAM