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Literatursammlung: Quantitative Auswertung von Dünnschicht-Chromatogrammen
Journal
of’Chrontatogruphy,
261 (1983) 187
Elsevier Science Publishers B.V., Amsterdam ~ Printed in the Netherlands CHROM. 15,838
Book
Review
Quantitative Auswertung VOW Diinnschicht-ChromatograPnPne*, by H. Jo& and H. Wimmer, G-1-T Verlag Ernst Giebeler, Darmstadt, 1st instalment, 1982, VIII + 96 pp., subscription price DM 82.00 per instalment (three instalments per year).
Literatursammlung;
In their preface the authors state only too correctly that “in discussions about TLC with workers in the field the question ‘But where can all this information be found?’ soon crops up. This happens every time with Participants in workshops. There is an obvious lack in the literature of praCtiCa1 introductions to the field”. They go on to say that in order to fill this gap they have decided to start this compilation in the form of a loose-leaf folder, which will be supplemented three times a year. In the first instalment, they supply five sheets on general topics and forty on specific determinations, mostly condensed from original papers which had appeared in the usual journals. The volume is being edited by one of the greatest authorities on quantitative TLC, namely Professor H. Jork, and one can only be grateful that he has undertaken the task. A quick perusal will show the reader that there are numerous quantitative TLC methods with an order of accuracy of f 5%, which is adequate for most purposes and rarely bettered by costly instrumental HPLC methods. This reviewer would like to raise two points. The first is that the authors feel that most chromatographers are unaware of these methods; this may hold true for beginners but if they regularly read the literature, they should not be. However, as most are convinced that instrumental chromatography is the modern way of life, they would not even take such TLC techniques into consideration. The second point is that no mention is made of paper chromatography. True, TLC experts will insist that paper chromatography is a poorer form of TLC, and this is sometimes, but certainly not always, true. But while paper chromatography has often less theoretical plates, it does permit work with larger volumes and thus more precise quantitation methods. The first example of such to come to mind is the masterly automatic methods devised by Professor Ian Bush for the determination of steroids in biological fluids, The second example is an argentometric determination of LiCl, NaCl and KCl, after separation on a paper sheet using pure methanol as solvent. Not to speak of the numerous calorimetric and polarographic determinations for transition metal ions. And what about the quantitative determination of serum proteins after separation by paper electrophoresis, which held its own as a diagnostic routine method for more than ten years? However, all this may seem to many chromatographers of historical interest Only. soy perhaP& alsO Professor Jerk may findthat nowadays instrumental is beau_ tiful and that there is little his compilation can do to change this, quantitative
of’Chrontatogruphy,
261 (1983) 187
Elsevier Science Publishers B.V., Amsterdam ~ Printed in the Netherlands CHROM. 15,838
Book
Review
Quantitative Auswertung VOW Diinnschicht-ChromatograPnPne*, by H. Jo& and H. Wimmer, G-1-T Verlag Ernst Giebeler, Darmstadt, 1st instalment, 1982, VIII + 96 pp., subscription price DM 82.00 per instalment (three instalments per year).
Literatursammlung;
In their preface the authors state only too correctly that “in discussions about TLC with workers in the field the question ‘But where can all this information be found?’ soon crops up. This happens every time with Participants in workshops. There is an obvious lack in the literature of praCtiCa1 introductions to the field”. They go on to say that in order to fill this gap they have decided to start this compilation in the form of a loose-leaf folder, which will be supplemented three times a year. In the first instalment, they supply five sheets on general topics and forty on specific determinations, mostly condensed from original papers which had appeared in the usual journals. The volume is being edited by one of the greatest authorities on quantitative TLC, namely Professor H. Jork, and one can only be grateful that he has undertaken the task. A quick perusal will show the reader that there are numerous quantitative TLC methods with an order of accuracy of f 5%, which is adequate for most purposes and rarely bettered by costly instrumental HPLC methods. This reviewer would like to raise two points. The first is that the authors feel that most chromatographers are unaware of these methods; this may hold true for beginners but if they regularly read the literature, they should not be. However, as most are convinced that instrumental chromatography is the modern way of life, they would not even take such TLC techniques into consideration. The second point is that no mention is made of paper chromatography. True, TLC experts will insist that paper chromatography is a poorer form of TLC, and this is sometimes, but certainly not always, true. But while paper chromatography has often less theoretical plates, it does permit work with larger volumes and thus more precise quantitation methods. The first example of such to come to mind is the masterly automatic methods devised by Professor Ian Bush for the determination of steroids in biological fluids, The second example is an argentometric determination of LiCl, NaCl and KCl, after separation on a paper sheet using pure methanol as solvent. Not to speak of the numerous calorimetric and polarographic determinations for transition metal ions. And what about the quantitative determination of serum proteins after separation by paper electrophoresis, which held its own as a diagnostic routine method for more than ten years? However, all this may seem to many chromatographers of historical interest Only. soy perhaP& alsO Professor Jerk may findthat nowadays instrumental is beau_ tiful and that there is little his compilation can do to change this, quantitative