CRYOBIOLOGY
17, 631-639
(1980)
Subject
Index’
for Volume
A
Bark and xylem, twigs: freezing patterns (peach), 17, 495 Bilayer liposome, freeze-thaw stability, 17, 508 Biological materials dry, determination of residual moisture, in final period of freeze-drying, 17, 585 Blood frozen, immunocompetence of residual lymphocytes (human), 17, 100 Bone mandible, cryosurgery: light and electron microscopy (rabbit), 17, 563 Bone marrow cells cadaver, cryopreservation, 17, 419 granulocyte precursors: survival measured by CFU-c (rat), 17, 262 cryopreservation, long term: viability after (human), 17, 424 erythropoietin-responsive cells, effect of cryopreservation (mouse), 17, 18 frozen: morphological, cytochemical, and culture study (human), 17, 429 hemopoietic stem cells, role of serum in cryopreservation and viability (mouse), 17, 516 Book reviews Cold Tolerant Microbes in Spoilage and the Environment, A. D. Russell and R. Fuller (Eds.). Reviewed by 0. Fennema and E. H. Marth, 17, 521 Fundamentals of Freeze-Drying, J. D. Mellor. Reviewed by D. Greiff, 17, 418 Bulb epidermis cells, nucleation sites for freezing and refreezing (onion), 17, 184 Burst-forming unit assay, after long-term marrow cryopreservation (human), 17, 424
Abstracts papers presented at 17th annual meeting of Society for Cryobiology, 17, 593 16th annual meeting of Society for Cryobiology [erratum to 16, 582 (1979)], 17, 318 Adenosinetriphosphatase Ca’+-dependent, activity: thylakoid membranes during freezing (spinach), 17, 486 Adenosine 5’-triphosphate effect on protection of functions of polymorphonuclear neutrophils during preservation (guinea pig), 17, 108, 112 in storage media, effect on skin preservation (human), 17, 46 Adrenal medulla: electron microscopy, effect of hibernation (hedgehog), 17, 74 Adrenalin, see Epinephrine Agar culture, frozen bone marrow (human), 17, 429 Albumin effect on protection of functions of polymorphonuclear neutrophils (guinea pig) during preservation (cattle), 17, 108, 112 Allograft skin, procurement and processing (human), 17, 33 Anions impermeable, effect on kidney cortex slice volume and ion distribution at hypothermia (dog), 17, 540 Anticoagulant interaction, in granulocyte preservation, 17, 187 Autoradiography frozen-thawed granulocytes (guinea pig), 17, 1 B Bacteria Escherichia coli, induction of A phage by freezedrying, 17, 181 Bactericidal activity frozen granulocytes (rat), 17, 262 Bactericidal capacity granulocyte concentrates for transfusion, effects of liquid and cryopreservation (dog), 17, 252 Bacteriophage A, induction in lysogenic bacteria by freeze-drying, 17, 181 ’ Boldface numbers indicate volume; lightface
17
C Cadaver bone marrow cells, cryopreservation, 17, 419 Calcium CaL+-ATPase, activity: thylakoid membranes during freezing (spinach), 17, 486 Cancer prostate: cryoimmunotherapy, assessment of candidacy (human), 17, 170
numbers indicate pagination. 631 Copyright AU rights
@ 1980 by Academic Ress, Inc. of reproduction in any form reserved.
632
SUBJECT
Carcinoma kidney epithelial cells, postfreeze viability in quaternary solutions (human), 17, 363 Cat endoesophageal cryovagotomy, 17, 161 Catecholamines storage, seasonal changes (hedgehog), 17, 74 Cattle albumin, effect on protection of functions of polymorphonuclear neutrophils (guinea pig) during preservation, 17, 108, 112 spermatozoa, evaluation of cryoprotective compounds, 17, 458 Cell suspensions, low-temperature preservation: osmotic lysis as damaging factor during, 17, 403 volume in hyper- and hypotonic solution, 17, 403 Centrifugation sperm, looped tails after (human), 17, 25 Chemotaxis granulocyte concentrates for transfusion, effects of liquid and cryopreservation (dog), 17, 252 in viability assays after granulocyte preservation, 17, 187 Chloroplast coupling factor, release and inactivation, effects of freezing (spinach), 17, 486 thylakoids, freezing injury (Spinach), 17, 486 Colony-forming unit assay, after long-term marrow cryopreservation (human), 17, 424 spleen, assay: viability test for transplantable hemopoietic tissue, 17, 516 Concanavalin A capping, lymphocytes: effect of cryopreservation (human), 17, 12 Cooling rate, effect on survival of lymphocyte subpopulations (mouse), 17, 439 Counterflow centrifugation-elutriation granulocyte isolation by (human), 17, 230, 243 Cryobiology kidney cortex, mechanisms of injury (rabbit), 17, 371 Cryoimmunotherapy prostate cancer, assessment of candidacy (human), 17, 170 Cryopreservation bone marrow cells, cadaver, 17, 419 hemopoietic stem cells, role of serum, 17, 516 erythropoietin-responsive cells in hematopoietic tissue (mouse), 17, 18 Ficoll-Hypaque isolated granulocytes (human), 17, 287 granulocytes factors affecting postthaw stability (human), 17, 297
INDEX
in viva distribution (guinea pig), 17, 1 for transfusion, effects on in vitro function (dog), 17, 252 for transfusion (human, rabbit), 17, 198 -induced changes in lymphocytes (human), 17, 12 kidney cortex (rabbit), 17, 371 metabolic changes during (dog), 17, 549 long-term, marrow viability after (human), 17, 424 lymphocytes decreased response in immune assays in sarcoma patients, 17, 93 subpopulations, microprocessor-controlled cooling system (mouse), 17, 439 platelets, survival and recovery (rat), 17, 589 skin graft, viability testing (swine), 17, 130 package format, cooling and warming rates, and storage efficiency (human), 17, 33 Cryoprotectants glycerol or dimethyl sulfoxide, skin viability after freezing (swine), 17, 130 Cryoprotection erythrocytes, and osmotic volume reduction (human), 17, 530 lymphocyte subpopulations, microprocessorcontrolled cooling system (mouse), 17, 439 semen, comparison of glycerol or egg yolk-citrate-glycerol (human), 17, 25 thylakoids, by sugars (spinach), 17, 486 Cryoprotective additives in granulocyte cryopreservation for transfusion (human, rabbit), 17, 198 Cryoprotective agents dual, kidney epithelial carcinoma cells in quaternary solutions (human), 17, 363 effects on liposome bilayers, stabilization to freezing and thawing, 17, 508 glucose, sucrose, and dimethyl sulfoxide: comparison of survival rates after cryopreservation (hemoprotozoan), 17, 410 in granulocyte preservation, 17, 187 potassium chloride, erythrocytes (human), 17, 530 Cryoprotective compound spermatozoa, evaluation (cattle), 17, 458 Cryosurgery bone, mandible: light and electron microscopy (rabbit), 17, 563 current flow and tissue temperature (dog), 17, 154 prostate (human), 17, 170 Cryotherapy prostatic cancer (human), 17, 170 Cryovagotomy endoesophageal (cat), 17, 161 Current flow, in cryosurgery (dog), 17, 154 Cytochemistry frozen bone marrow (human), 17, 429
““““-7
JVLWCLI
633
INDEX
D
E
Desorption in freeze-drying, determination of residual moisture in dry biological materials, 17, 585 Dielectric properties kidney (dog), 17, 393 Dielectric temperature, see Temperature Differential thermal analysis study on cryoprotective action of hydroxyethyl starch, 17, 54 Dilution rapid, embryos (mouse), 17, 178 Dimethyl sulfoxide in cryopreservation of granulocyte concentrates for transfusion (dog), 17, 252 cryoprotection compared with glucose and sucrose, survival rates (hemoprotozoan), 17, 410 kidney cortex (rabbit), 17, 371 skin viability after freezing (swine), 17, 130 glycerol, ethylene glycerol in combination with glucose, ATP, or albumin: protection of neutrophil functions (guinea pig), 17, 112 granulocytes cryopreservation for transfusion (human, rabbit), 17, 198 frozen: viability (rat), 17, 262 perfusion: frozen kidney, microwave thawing (dog), 17, 393 toxicity, during freezing: kidney cortex (rabbit), 17, 371
Effective dielectric temperature, see Temperature E.% yolk, liposome bilayers: freeze-thaw stability, 17, 508 Egg yolk-citrate-glycerol cryoprotection for semen (human), 17, 25 Electrolyte intracellular, erythrocytes: relation to freezing injury (human), 17, 530 Electronic device with turbine, for determination of residual moisture of dry biological materials, 17, 585 Electron microscopy cryosurgery on bone (rabbit), 17, 563 evaluation of rapid freezing techniques, kidney slices (rat), 17, 571 Electrophoretic mobility technique, freezing patterns in twig bark and xylem (peach), 17, 495 Energy transfer between bilayer probes, 17, 508 Enzyme in vitro analysis, stored granulocytes (human), 17, 243 lipoxygenase, activity in linolenic acid oxidation at subfreezing temperatures (soybean), 17, 500 Epinephrine in adrenal medulla, effect of hibernation (hedgehog), 17, 74 Epithelium embryo, unprotected cell cultures: effect of hypertonicity on postthaw survival (human), 17, 120 Erythrocytes freezing injury, relationship to osmotic volume reduction (human), 17, 530 frozen in glycerol, ultrastructure (human), 17, 448 hydroxyethyl starch, minimum effective concentration, 17, 54 membrane damage, caused by intracellular ice (human), 17, 351 Erythropoietin-responsive cells bone marrow and fetal liver, effect of cryopreservation (mouse), 17, 18 Esophagus cryovagotomy (cat), 17, 161 Eutectic trough water-sodium chloride-hydroxyethyl starch system, 17,54
Dog frozen kidney, microwave thawing, 17, 393 granulocyte concentrates for transfusion, effects of liquid and cryopreservation on in vitro function, 17, 252 kidney cortex slice volume and ion distribution, effect of impermeable anions at hypothermia, 17, 540 cryopreservation: metabolic changes during, 17, 549 microwave illumination for thawing, 17, 465 mitochondria, effects of hypothermia, 17, 148 preservation by hypothermic perfusion, effect of substrate on energy metabolism, 17, 135 preservation, normothermic ex vivo perfusion, 17, 473 lung transplantation, effect of warm ischemia and hypothermic storage, 17, 389 skin, current flow and tissue temperature in cryosurgery, 17, 154 Dopamine p-hydroxylase, see Dopamine p-monooxygenase Dopamine /3-monooxygenase increase during hibernation (hedgehog), 17, 74 Drying, see Freeze-drying
F Ficoll - Hypaque isolation of granulocytes, before cryopreservation (human), 17, 287 Fluorescence-activated cell sorter surface immunoglobulin density on frozen-thawed mononuclear cells (human), 17, 523
634
SUBJECT
Freeze-drying in evaluation of rapid freezing techniques, kidney slices (rat), 17, 571 induction of A bacteriophage in lysogenic bacteria by, 17, 181 lymphocytes (human), 17, 319 and residual moisture of dry biological materials, 17, 585 Freeze-etching erythrocyte membrane damage caused by intracellular ice (human), 17, 351 Freeze-fixation for electron microscopy, kidney slices (rat), 17, 571 Freeze-fracture erythrocyte membrane damage caused by intracellular ice (human), 17, 351 replicas, erythrocytes frozen with glycerol under clinical conditions (human), 17, 448 Freeze-preservation granulocytes (human), 17, 311 Freeze-thaw stability, liposome bilayers, 17, 508 Freezing granulocytes effect of cooling rate (human), 17, 282 human, rabbit, 17, 198 injury erythrocyte membranes, caused by intracellular ice (human), 17, 351 erythrocytes, relationship to osmotic volume reduction (human), 17, 530 large unit, erythrocytes in glycerol for clinical use (human), 17, 448 to -196”C, skin graft viability testing (swine), 17, 130 patterns, twig bark and xylem (peach), 17, 495 rapid, techniques: evaluation with kidney slices (rat), 17, 571 rate, skin (human), 17, 33 repeated: nonwoody plant tissues, nucleation sites, 17, 184 two-step embryo preservation (mouse), 17, 178 granulocytes, viability (rat), 17, 262 Freezing microscope observation of repeated freezing of bulb epidermis cells (onion), 17, 184 Frozen bone marrow: morphological, cytochemical, and culture study (human), 17, 429 erythrocytes, in glycerol for clinical use (human), 17, 448 kidneys, microwave illumination for thawing (dog), 17, 465 organs, thermal stresses in, 17, 66, 522 samples, lipoxygenase activity in linolenic acid oxidation, 17, 500
INDEX Frozen-thawed mononuclear cells, surface immunoglobulin determination (human), 17, 523
density
G Glucose cryoprotection compared with sucrose and dimethyl sulfoxide, survival rates (hemoprotozoan), 17, 410 P-Glucuronidase as indicator of damage to lysosomes, 17, 198 Glutamic-oxaloacetic transaminase enzyme retention, measurement of spermatozoa membrane integrity (cattle), 17, 458 Glycerol cryoprotection, skin viability after freezing (swine), 17, 130 effect on lysosomes of granulocytes, 17, 198 erythrocytes frozen in, for clinical use: ultrastructure (human), 17, 448 in granulocyte cryopreservation for transfusion (human, rabbit), 17, 198 3H labeled: rate of flux, overy cells (hamster), 17, 338 toxicity to granulocytes (human), 17, 282 Glycerolization ovary cells (hamster), 17, 338 Graft skin, viability testing after freezing (swine), 17, 130 Granulocytes concentrates for transfusion, liquid and cryopreservation effects on in vitro function (dog), 17,252 cryopreservation distribution in vivo (guinea pig), 17, 1 factors affecting postthaw stability (human), 17, 297 after Ficoll-Hypaque isolation (human), 17, 287 phagocytosis by, in vitro (guinea pig), 17, 1 for transfusion (human, rabbit), 17, 198 frozen, viability (rat), 17, 262 function effect ofstorage conditions (human), 17, 222 after freeze-preservation (human, 17, 311 harvesting, by filtration leukapheresis (rat), 17, 213 injury at low temperatures (human), 17, 273 isolation by counterflow centrifugation (human), 17, 297 counterflow centrifugation-elutriation (human), 17, 230 preservation conference summary, 17, 187 cryogenic temperatures (-80°C) (guinea pig), 17, 112 by freezing, approaches (human), 17, 282 low temperature (4°C) (guinea pig), 17, 108 purified, liquid preservation (human), 17, 243 Guinea pig glycogen-induced exudates, polymorphonuclear neutrophils: preservation, 17, 108, 112
SUBJECT granulocytes, in viva distribution vation, 17, 1 Ii
after cryopreser-
Hamster Chinese, ovary cells: monolayer culture, glycerolization, 17, 338 Hedgehog Erinaceus europaeus, adrenal medulla: effect of hibernation on catecholamines, ATP, and dopamine P-hydroxylase, 17, 74 Hemopoietic stem cells bone marrow, role of serum in cyropreservation and viability (mouse), 17, 516 Hemoprotozoan Babesia rodhaini, survival rates after cryopreservation, 17, 410 Hibernation effect on catecholamines, ATP, and dopamine phydroxylase in adrenal medulla (hedgehog), 17, 74 Hydroxyethyl starch in cryopreservation of granulocytes concentrates for transfusion (dog), 17, 252 cryoprotective properties, differential thermal analysis, 17, 54 granulocytes, frozen: viability (rat), 17, 262 water-absorptive capacity, 17, 54 Hypertonicity effect on postthaw survival of cultured cells (human), 17, 120 Hypothermia cell swelling, control in kidney cortex slices by impermeable anions (dog), 17, 540 effects on kidney mitochondria (dog), 17, 148 Hypothermic storage effect on lung transplantation (dog), 17, 389 I Ice intracellular, erythrocyte membrane damage caused by (human), 17, 351 sublimation in vacua, lymphocyte drying by (human), 17, 319 Illumination microwave, for thawing frozen kidneys (dog), 17, 465 Immune assays decreased response of cryopreserved lymphocytes in sarcoma patients, 17, 93 Immunobiology lymphocytes, after freeze-drying (human), 17, 319 Immunocompetence residual lymphocytes in frozen blood (human), 17, 100 Immunoglobulin surface, density: frozen-thawed mononuclear cells (human), 17, 523 Injury granulocytes, at low temperatures (human), 17, 273
635
INDEX
Ischemia cold, effects on distribution of perfusate in preserved kidney (rat), 17, 478 warm, effect on lung transplantation (dog), 17, 389 J Jaw mandible, cryosurgery
on bone (rabbit), K
17, 563
Kidney cortex cryopreservation (rabbit), 17, 371 slice volume and ion distribution, effects of impermeable anions at hypothermia (dog), 17,540 slices, in evaluation of rapid freezing techniques (rat), 17, 571 cryopreservation, metabolic changes during (dog), 17, 549 distribution of perfusate, effects of cold ischemia (rat), 17, 478 epithelial carcinoma cells, postfreeze viability in quaternary solutions (human), 17, 363 frozen microwave illumination for thawing (dog), 17,465 microwave thawing (dog), 17, 393 mitochondria, effects of hypothermia (dog), 17, 148 preservation effect on polysomal messenger RNA (mouse), 17, 557 by hypothermic perfusion, effect of substrates on energy metabolism (dog), 17, 135 long-term (144 hr): normothermic ex viva perfusion (dog), 17, 473 L Leaf chloroplast coupling factor, effects of freezing (spinach), 17, 486 Lethal salt enrichment prevention by hydroxyethyl starch, 17, 54 Leukapheresis centrifuge or filtration, effect on granulocyte function (human), 17, 222 continuous flow centrifugation, granulocyte isolation by (human), 17, 230 filtration, kinetics of neutrophil-releasing activity of (rat), 17, 213 Leukemia chronic granulocytic, viability of cryopreserved marrow (human), 17, 424 Leukocytes polymorphonuclear frozen, viability (rat), 17, 262 in granulocyte preservation conference summary, 17, 187 Linolenic acid oxidation, with lipoxygenase in frozen samples, 17, 500 Liposome bilayers, egg yolk: freeze-thaw stability, 17, 508
636
SUBJECT INDEX
Lipoxygenase activity in linolenic acid oxidation in frozen samples (soybean), 17, 500 Liquid preservation granulocytes, effects on in vitro function (dog), 17, 252 purified granulocytes (human), 17, 243 Liver erythropoietin-responsive cells, fetus: effect of cryopreservation (mouse), 17, 18 Lung transplantation, effect of warm ischemia and hypothermic storage (dog), 17, 389 Lymphocytes B, frozen-thawed: surface immunoglobulin density (human), 17, 523 cryopreservation (human), 17, 523 cryopreserved: decreased response in immune assays in sarcoma patients, 17, 93 freeze-drying, immunobiology after (human), 17, 319 residual, in frozen blood: immunocompetence (human), 17, 100 spleen, effects of six cooling rates on viability and function (mouse), 17, 439 structure and functional alterations induced by cryopreservation (human), 17, 12 Lyophilization, see Freeze-drying Lysosomes as indicators of cryoinjury in granulocytes after cryopreservation, 17, 198 M
Mandible cryosurgery on bone (rabbit), 17, 563 Marrow, see Bone marrow Membrane damage, erythrocytes: caused by intracellular ice (human), 17, 351 thylakoid, freezing injury (spinach), 17, 486 transport, integrity: kidney slices, effect of cryopreservation (dog), 17, 549 ultrastructure, erythrocytes: freeze-fracture and etching (human), 17, 351 Metabolism energy, hypothermic: in perfused kidneys, effects of substrate (dog), 17, 135 Microprocessor -controlled cooling system, cryoprotection of lymphocyte subpopulations (mouse), 17, 439 Microwave illumination, for thawing frozen kidneys (dog), 17, 465 thawing, frozen kidneys (dog), 17, 393 Mitochondria kidney, effects of hypothermia (dog), 17, 148
Moisture residual, determination in final period of freezedrying: dry biological materials, 17, 585 Mononuclear cells frozen-thawed, determination of surface immunoglobulin density (human), 17, 523 Motility post-thaw, sperm (human), 17, 25 progressive, measure of cryoprotective effect on spermatozoa (cattle), 17, 458 Mouse bone marrow and fetal liver, erythropoietin-responsive cells: effect of cryopreservation, 17, 18 hemopoietic stem cells, role of serum in cryopreservation and viability, 17, 516 embryo, preservation by two-step freezing, 17, 178 kidney preservation, effect on polysomal messenger RNA, 17, 557 lymphocyte subpopulations, cryoprotection using microprocessor-controlled cooling system, 17, 439 N
Natural killer activity lymphocytes, effect of cryopreservation (human), 17, 12 Neutrophils polymorphonuclear chemotaxis, trypan blue exclusion, phagocytosis, adhesiveness, cyanide-insensitive oxygen consumption (guinea pig), 17, 108, 112 from glycogen-induced exudates, preservation (guinea pig), 17, 108, 112 -releasing activity of filtration leukapheresis, kinetics (rats), 17, 213 Nitroblue tetrazolium in viability assays after granulocyte preservation, 17, 187 of frozen granulocytes (rat), 17, 262 Nitrogen liquid, vapor phase: long-term marrow cryopreservation (human), 17, 424 Noncontact measurement, effective dielectric temperature, 17, 465 Nucleation sites, for freezing and refreezing bulb epidermis cells (onion), 17, 184 0 Onion bulb epidermis cells, nucleation sites for freezing and refreezing, 17, 184 Organ cryopreservation, kidney (dog), 17, 393 frozen, mechanical damage from thermal stresses, 17, 66, 522
SUBJECT preservation, effect on polysomal messenger RNA (mouse), 17, 557 Osmolality effect on glycerol flux, ovary cells (hamster), 17,338 Osmotic lysis model, damaging factor during low-temperature preservation of cell suspensions, 17, 403 Osmotic stress and erythrocyte freezing injury (human), 17, 530 Ovary cells, glycerolization (hamster), 17, 338 Ovens microwave illumination for thawing kidneys, evaluation (dog), 17, 465 Oxidation linolenic acid with lipoxygenase in frozen samples, 17, 500 P Package geometry, for freezing transplantable skin (human), 17, 33 Peach twigs, bark and xylem: freezing patterns, 17, 495 Perfusate distribution in preserved kidney, effect of cold ischemia (rat), 17, 478 Perfusion dimethyl sulfoxide: frozen kidney, microwave thawing (dog), 17, 393 ex viva, normothermic: long-term kidney preservation (144 hr) (dog), 17, 473 hypothermic, kidney: effect of substrate on energy metabolism (dog), 17, 135 Peritoneum exudate, polymorphonuclear leukocytes: functional survival after freezing (rat), 17, 262 Phagocytosis granulocyte concentrates for transfusion, effects of liquid and cryopreservation (dog), 17, 252 test for viability after granulocyte cryopreservation (human, rabbit), 17, 198 in viability assays after granulocyte preservation, 17, I87 Physiological function in vitro analysis, stored ‘granulocytes (human), 17, 243 Phytohemagglutinin -stimulated blastogenic transformation: lymphocytes, effect of cryopreservation (human), 17, 12 Plasma postleukapheresis, physiologic properties (rat), 17, 213 survival and recovery after cryopreservation (rat), 17, 589 Polymorphonuclear neutrophils, see Neutrophils
637
INDEX
Polyribosome messenger RNA, effect of organ preservation (mouse), 17, 557 Polysome, see Polyribosome Postleukapheresis plasma physiologic properties (rat), 17, 213 Postthaw survival of unprotected cell cultures, effect of hypertonicity (human), 17, 120 Potassium relationship to erythrocyte cell injury (human), 17, 530 Preservation, see also Cryopreservation embryos, by two-step freezing (mouse), 17, 178 granulocytes conference summary, 17, 187 by freezing (human), 17, 282 kidney effect on polysomal messenger RNA (mouse), 17, 557 long-term (144 hr): combination of standard machine and normothermic ex viva perfusion (dog), 17, 473 liquid granulocytes, effects on in vitro function (dog), 17, 252 purified granulocytes (human), 17, 243 low-temperature, cell suspensions: osmotic lysis as damaging factor during, 17, 403 skin, metabolic integrity (human), 17, 46 Prophage induction, by freeze-drying (bacteria), 17, 181 Prostate cancer: cryoimmunotherapy, assessment of candi* dacy (human), 17, 170 Protection method, cell suspensions during low-temperature preservation, 17, 403 Proteins membrane, stabilizing effect in liposome bilayers, 17, 508 synthesis, kidney slices: effect of cryopreservation (dog), 17, 549 cl Quatemary solutions postfreeze viability of kidney epithelial cells in (human), 17, 363
carcinoma
n Rabbit cryosurgery on bone, 17, 563 granulocytes cryopreserved in dimethyl sulfoxide, phagocytic activity, 17, 198 kidney cortex, frozen with dimethyl sulfoxide: solution effects injury, 17, 371
638
SUBJECT
skin, refrigerated storage: evaluation of media and viability, 17, 125 Radioautography, see Autoradiography Rat distribution of perfusate in preserved kidney, effects of cold ischemia, 17, 478 frozen granulocytes and granulocyte precursors, viability, 17, 262 kidney cortex slices, in evaluation of rapid freezing techniques, 17, 571 platelets, survival and recovery after cryopreservation, 17, 589 postleukapheresis plasma, physiologic properties, 17, 213 Recovery platelets, after cryopreservation (rat), 17, 589 Red blood cells, see Erythrocytes RNA messenger, polysome: effect of organ preservation (mouse), 17, 557 Rotor modified, for granulocyte isolation by counterflow centrifugation-elutriation (human), 17, 230 S Salt intracellular, concentration: erythrocytes, relationship with cell injury (human), 17, 530 Sarcoma cryopreserved lymphocytes: decreased response in immune assays (human), 17, 93 Semen liquid nitrogen cryoprotection, comparison of glycerol or egg yolk-citrate-glycerol (human), 17, 25 Separation chamber enlarged, for granulocyte isolation by counterflow centrifugation-elutriation (human), 17, 230 Serum role on cryopreservation and viability of bone marrow hemopoietic stem cells (mouse), 17, 516 Shock thermal, erythrocytes: relationship between cell injury and osmotic volume reduction (human), 17, 530 Skin banking: package format, cooling and warming rates, and storage efficiency (human), 17, 33 freezing damage (human), 17, 46 frozen, current flow and tissue temperature (dog), 17, 154 graft, viability testing after freezing (swine), 17, 130 preservation at -3 and - 196”C, metabolic changes (human), 17, 46 refrigerated, evaluation of storage conditions (rabbit), 17, 125 Solution
INDEX effects, in injury: analysis, kidney cortex (rabbit), 17, 371 Soybean lipoxygenase activity in linolenic acid oxidation in frozen samples, 17, 500 Spermatozoa cryoprotective compounds, evaluation (cattle), 17, 458 postthaw motility and ultrastructure damage (human), 17, 25 Spinach Spinacia olerucea, leafi chloroplast coupling factor, effects of freezing, 17, 486 Spleen lymphocytes, subpopulations: cryoprotection, using microprocessor-controlled cooling system (mouse), 17, 439 Stability freeze-thaw, liposome bilayers, 17, 508 postthaw, factors affecting: granulocytes (human), 17, 297 Stem cells hemopoietic, bone marrow: role of serum in cryopreservation and viability (mouse), 17, 516 Storage conditions, effect on granulocyte function (human), 17, 222 efficiency, transplantable skin (human), 17, 33 hypothermic, effect on lung transplantation (dog), 17, 389 refrigerated skin: evaluation of viability and storage media (rabbit), 17, 125 warm vs cold, kidney: effect on polysomal messenger RNA (mouse), 17, 557 Stresses thermal, in frozen organs, 17, 66, 522 Substrate supply, effect on perfused kidneys (dog), 17, 135 Sucrose cryoprotection compared with glucose and dimethyl sulfoxide, survival rates (hemoprotozoan), 17, 410 Sulfate radioactive, in skin graft viability testing after freezing (swine), 17, 130 Surface markers lymphocytes, effects of cryopreservation (human), 17, 12 Survival functional, granulocytes and granulocyte precursors (rat), 17, 262 platelets, after cryopreservation (rat), 17, 589 postthaw, unprotected cell cultures: effect of hypertonicity (human), 17, 120 rates, after cryopreservation (hemoprotozoan), 17, 410 Swine skin, cryopreservation: graft viability, 17, 130
SUBJECT T
Temperature effective dielectric, noncontact measurement: microwave illumination for thawing kidneys (dog), 17, 465 low, injury to granulocytes (human), 17, 273 Thawing electromagnetic, platelets (rat), 17, 589 frozen kidneys (dog), 17, 465 rate, frozen kidney (dog), 17, 393 uniformity, kidney (dog), 17, 393 Thermal stresses in frozen organs, 17, 66, 522 Thermocouple analysis, as evaluation of rapid freezing techniques, kidney slices (rat), 17, 571 Thermography evaluation of ovens: microwave illumination for thawing kidneys (dog), 17, 465 Thylakoids chloroplast, freezing injury (spinach), 17, 486 Transformation blastogenic, stimulated by phytohemagglutinin and allogeneic mononuclear cells: lymphocytes, effect of cryopreservation (human), 17, 12 Transfusion granulocyte concentrates for, effects of liquid and cryopreservation on in vitro function (dog), 17, 252 Transplantation lung, effect of warm ischemia and hypothermic storage (dog), 17, 389 Turbine in electronic device, for determination of residual moisture in dry biological materials, 17, 585 Twig bark and xylem, freezing patterns (peach), 17, 495 U
Ultrastructure erythrocytes, frozen with glycerol for clinical use (human), 17, 448
639
INDEX
kidney cortex slices, in evaluation of rapid freezing techniques (rat), 17, 571 V
Vagus nerve cryovagotomy, endoesophageal (cat), 17, 161 Valinomycin and erythrocyte permeability to potassium (human), 17, 530 Vascular resistance preserved kidney, effects of cold ischemia (rat), 17, 478 Viability assays, after granulocyte preservation, 17, 187 bone marrow after long-term cryopreservation (human), 17, 424 frozen granulocytes and granulocyte precursors (rat), 17, 262 functional, after granulocyte preservation, 17, 187 granulocytes, after freeze-perservation (human), 17, 311 postfreeze, kidney epithelial carcinoma cells in quaternary solutions (human), 17, 363 refrigerated skin (rabbit), 17, 125 stem cells, after cryopreservation: role of serum (mouse),
17, 516
Viscosity effect on glycerol flux, ovary cells (hamster), 17,338 Volume kidney cortex slice, effect of impermeable anions at hypothermia (dog), 17, 540 reduction, osmotic: erythrocytes, relationship with cell injury (human), 17, 530 w Warming rapid, embryos (mouse), 17, 178 Water-sodium chloride-hydroxyethyl system, phase diagram, 17, 54
starch
X Xylem twigs, freezing patterns (peach), 17, 495