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Volume 13, Part 2, May 1999 Acknowledgments. We thank C. J. K. Wang for providing assistance in the cultural and taxonomic aspects of this study. We also thank H. Fan for technical assistance and J. D. Castello for reviewing the manuscript.
Ice Core From Stor a ge Facility
I
11111/1/ I \ \ \'
~ III
~~~~~ Section Selected
Surface T rea ted with UV Irradiation (middle covered with aluminum foil) /
Inner Cor e Separated Fr om Outer Shell Aseptica lly @ _20°C
--lJ
References Abyzov, S. S. G. (1993) Micro-organisms in the Antarctic Filtered Su bsa mple ice, in Antarctic Microbiology (ed. E. 1. Friedman), pp. .. (ta ken through same processes as Unfi lter ed 265-295. New York: John Wiley & Sons, Inc. with unfiltered sam ples below) Suh sample Bartholomew, J. W. & Paik, G. (1966) Isolation and Inn er Co re Melted identificationofobligatethermophilic sporeformingbacilli @ 22°C (Stored @ .20°C) from ocean basin cores.Journal of Bacteriology92:635-638. Catranis, C. & Starmer, W. T. (1991) Micro-organisms ]BACT ERIA AND FUN GUS ASSAYS entrapped in glacial ice. Antarctic Journal of the VIRUS ASSAYS / \ United States 26: 234-236. Cultures Cross, T. & Atwell, R. W. (1974) Recovery of viable thermo actinomycete endospores from deep mud cores, in Spore Research (eds. A. N. Barker, G. W. 8 Media (with replicates) Gould & J. Wold), pp. 11-20.London: Academic Press. Gest,H. & Mandelstam, J. (1987)Longevity ofmicro-organisms Direct PCR Amplifica tion, Sequ encing in natural environments. Microbiological Sciences4:69-71. Various From Melted Ice Lindahl, T. (1993) Instability and decay of the primary Incuhation T imes and structure of DNA. Nature 362: 709-715. Temp eratures Liu, Y., Ammirati, J. F. & Rogers, S. O. (1997) Phylogenetic Relationships of Dermocybe and Cortinarius species based on nuclear ribosomal DNA Extra ction, Examinations PCR Am plilication, internal transcribed spacers. Canadian Journal of '" Sequencing " Botany. 75: 519-532. Characterize Sequ ence Com pa r isons Ma,1. J., Fan, H., Catranis, C.,Rogers,S. 0., & Starmer, W. T. and/or Identify .. (DNA Database Searches and (1997) Isolation and characterization of fungi entrapped Ph ylogenetic Ana lyses) in glacial ice. Inoculum 48(3):MSA Abstracts, p.23. Reeh, N., Johnsen, S. J. & Dahl-Jensen, D. (1985) Dating Fig 2 Protocol for ice core isolation, culturing, and DNA sequencing. the Dye 3 deep ice core by flow model calculations, in Sneath, P. H. A. (1962) Longevity of micro-organisms. Greenland Ice Core: Geophysics, geochemistry, and the Nature 195: 643-646. Environment, Geophysical Monograph 33 (eds. C. C. Swofford, D. (1993) PAUP: phylogenetic analysis using Langway, Jr., H. Oeschger & W. Dansgaard), pp. 57parsimony, Version 3.1.1, University of Illinois, 65. Washington, D. C.: American Geophysical Union. Champaign, 11. Rogers, S. O. & Bendich, A. J. (1985) Extraction of DNA Taylor, K. C., Mayewski, P. A., Alley, R. B., Brook, E. J., from milligram amounts of fresh, herbarium and Gow, A. J., Grootes, P. M., Meese, D. A., Saltzman, E. mummified plant tissues.Plant MolecularBiology 5:69-76. S., Severinghaus, J. P., Twickler, M. S., White, J. W. Seaward, A. C., Cross, T. & Unsworth, B. A. (1976)Viable C., Whitlow, S., & Zielinski, G. A. (1997) The bacterial spores recovered from an archaeological Holocene-Younger dryas transition recorded at excavation. Nature 261: 407-408. Summit, Greenland. Science 278: 825-827. Shi, T., Reeves, R. H., Gilichinsky, D. A. & Friedman, E. Vishniac, H. S. (1996) Biodiversity of yeasts and filamentous 1. (1997) Characterization of viable bacteria from microfungi in terrestrial Antarctic ecosystems. Siberian permafrost by 16S rDNA sequencing. Biodiversity and Conservation 5:1365-1378. Microbial Ecology 33: 169-179.
~
~ /
+
Micros~ 1~
The BMS Homepage The following are recent additions and amendments to the BMS Homepage (underlined entries are links): • • • • •
Foray Box (References for Identifying Fungi), has been updated to January 1999. Library: Books, has been reformatted and updated to 1994by integrating a supplement. Below Ground Research at York describes their work on roots and mycorrhizas. The Warwickshire Fungus Survey has been added to Fungus Groups. Photographs of fungi: The Quoditch Moor Nature Research has a nice selection. RTM
•
Ice Core From Stor a ge Facility
I
11111/1/ I \ \ \'
~ III
~~~~~ Section Selected
Surface T rea ted with UV Irradiation (middle covered with aluminum foil) /
Inner Cor e Separated Fr om Outer Shell Aseptica lly @ _20°C
--lJ
References Abyzov, S. S. G. (1993) Micro-organisms in the Antarctic Filtered Su bsa mple ice, in Antarctic Microbiology (ed. E. 1. Friedman), pp. .. (ta ken through same processes as Unfi lter ed 265-295. New York: John Wiley & Sons, Inc. with unfiltered sam ples below) Suh sample Bartholomew, J. W. & Paik, G. (1966) Isolation and Inn er Co re Melted identificationofobligatethermophilic sporeformingbacilli @ 22°C (Stored @ .20°C) from ocean basin cores.Journal of Bacteriology92:635-638. Catranis, C. & Starmer, W. T. (1991) Micro-organisms ]BACT ERIA AND FUN GUS ASSAYS entrapped in glacial ice. Antarctic Journal of the VIRUS ASSAYS / \ United States 26: 234-236. Cultures Cross, T. & Atwell, R. W. (1974) Recovery of viable thermo actinomycete endospores from deep mud cores, in Spore Research (eds. A. N. Barker, G. W. 8 Media (with replicates) Gould & J. Wold), pp. 11-20.London: Academic Press. Gest,H. & Mandelstam, J. (1987)Longevity ofmicro-organisms Direct PCR Amplifica tion, Sequ encing in natural environments. Microbiological Sciences4:69-71. Various From Melted Ice Lindahl, T. (1993) Instability and decay of the primary Incuhation T imes and structure of DNA. Nature 362: 709-715. Temp eratures Liu, Y., Ammirati, J. F. & Rogers, S. O. (1997) Phylogenetic Relationships of Dermocybe and Cortinarius species based on nuclear ribosomal DNA Extra ction, Examinations PCR Am plilication, internal transcribed spacers. Canadian Journal of '" Sequencing " Botany. 75: 519-532. Characterize Sequ ence Com pa r isons Ma,1. J., Fan, H., Catranis, C.,Rogers,S. 0., & Starmer, W. T. and/or Identify .. (DNA Database Searches and (1997) Isolation and characterization of fungi entrapped Ph ylogenetic Ana lyses) in glacial ice. Inoculum 48(3):MSA Abstracts, p.23. Reeh, N., Johnsen, S. J. & Dahl-Jensen, D. (1985) Dating Fig 2 Protocol for ice core isolation, culturing, and DNA sequencing. the Dye 3 deep ice core by flow model calculations, in Sneath, P. H. A. (1962) Longevity of micro-organisms. Greenland Ice Core: Geophysics, geochemistry, and the Nature 195: 643-646. Environment, Geophysical Monograph 33 (eds. C. C. Swofford, D. (1993) PAUP: phylogenetic analysis using Langway, Jr., H. Oeschger & W. Dansgaard), pp. 57parsimony, Version 3.1.1, University of Illinois, 65. Washington, D. C.: American Geophysical Union. Champaign, 11. Rogers, S. O. & Bendich, A. J. (1985) Extraction of DNA Taylor, K. C., Mayewski, P. A., Alley, R. B., Brook, E. J., from milligram amounts of fresh, herbarium and Gow, A. J., Grootes, P. M., Meese, D. A., Saltzman, E. mummified plant tissues.Plant MolecularBiology 5:69-76. S., Severinghaus, J. P., Twickler, M. S., White, J. W. Seaward, A. C., Cross, T. & Unsworth, B. A. (1976)Viable C., Whitlow, S., & Zielinski, G. A. (1997) The bacterial spores recovered from an archaeological Holocene-Younger dryas transition recorded at excavation. Nature 261: 407-408. Summit, Greenland. Science 278: 825-827. Shi, T., Reeves, R. H., Gilichinsky, D. A. & Friedman, E. Vishniac, H. S. (1996) Biodiversity of yeasts and filamentous 1. (1997) Characterization of viable bacteria from microfungi in terrestrial Antarctic ecosystems. Siberian permafrost by 16S rDNA sequencing. Biodiversity and Conservation 5:1365-1378. Microbial Ecology 33: 169-179.
~
~ /
+
Micros~ 1~
The BMS Homepage The following are recent additions and amendments to the BMS Homepage (underlined entries are links): • • • • •
Foray Box (References for Identifying Fungi), has been updated to January 1999. Library: Books, has been reformatted and updated to 1994by integrating a supplement. Below Ground Research at York describes their work on roots and mycorrhizas. The Warwickshire Fungus Survey has been added to Fungus Groups. Photographs of fungi: The Quoditch Moor Nature Research has a nice selection. RTM
•