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Ubiquitin-like Protein Hub1 Is Required for pre-mRNA Splicing and Localization of an Essential Splicing Factor in Fission Yeast
Current Biology 16, 2488, December 19, 2006 ª2006 Elsevier Ltd All rights reserved
Erratum Ubiquitin-like Protein Hub1 Is Required for pre-mRNA Splicing and Localization of an Essential Splicing Factor in Fission Yeast Caroline R.M. Wilkinson,* Gunnar A.G. Dittmar, Melanie D. Ohi, Peter Uetz, Nic Jones, and Daniel Finley (Current Biology 14, 2283–2288; December 29, 2004) In this paper, the authors showed that the ubiquitin-like protein Hub1 is required for pre-mRNA splicing in S. pombe and that its loss also affects localization of Snu66 in this yeast. As pointed out in the paper, the splicing defects observed in the Sphub1-4 mutant precede loss of nuclear localization of SpSnu66, and thus although nuclear localization of Snu66 is dependent on Hub1 function, the Hub1-Snu66 interaction does not function solely to effect nuclear localization. In budding yeast, Snu66 was shown to be mislocalized in a hub1 null mutant (data shown in Figure S4 originally published with the paper online). While carrying out new experiments, the authors realized that the Schub1 null strain used in this experiment carried a nonfluorescent GFP tag on ScSnu66. This led to the misinterpretation that ScSnu66-GFP loses its nuclear localization upon loss of HUB1. Having remade the hub1D SNU66-GFP strain, the authors have now observed that in budding yeast, Snu66-GFP is localized in the nucleus in both the presence and absence of Hub1 (revised Figure S4, shown here). Although this aspect of Hub1 function does not, therefore, seem to be conserved between these two yeasts, the main findings of the paper remain intact, specifically that Hub1 is required for pre-mRNA splicing in S. pombe and that its loss also affects localization of Snu66 in this yeast. Figure S4 and its legend, shown here, have also been corrected in the Supplemental Data available online. The authors regret and apologize greatly for any inconvenience and confusion that this error may have caused to others.
Figure S4. Localization of Snu66-GFP in S. cerevisiae Cells expressing the Snu66-GFP fusion protein in wild-type cells ([A] yGD319, Snu66-GFP::kanMX4) and in the Dhub1 background ([B] yGD318, Snu66-GFP::kanMX4 hub1::natMX6). The upper panel shows the cells in phase contrast, and the lower panel shows the fluorescence GFP signal of the Snu66-GFP fusion.
*Correspondence: [email protected]
DOI: 10.1016/j.cub.2006.12.004
Erratum Ubiquitin-like Protein Hub1 Is Required for pre-mRNA Splicing and Localization of an Essential Splicing Factor in Fission Yeast Caroline R.M. Wilkinson,* Gunnar A.G. Dittmar, Melanie D. Ohi, Peter Uetz, Nic Jones, and Daniel Finley (Current Biology 14, 2283–2288; December 29, 2004) In this paper, the authors showed that the ubiquitin-like protein Hub1 is required for pre-mRNA splicing in S. pombe and that its loss also affects localization of Snu66 in this yeast. As pointed out in the paper, the splicing defects observed in the Sphub1-4 mutant precede loss of nuclear localization of SpSnu66, and thus although nuclear localization of Snu66 is dependent on Hub1 function, the Hub1-Snu66 interaction does not function solely to effect nuclear localization. In budding yeast, Snu66 was shown to be mislocalized in a hub1 null mutant (data shown in Figure S4 originally published with the paper online). While carrying out new experiments, the authors realized that the Schub1 null strain used in this experiment carried a nonfluorescent GFP tag on ScSnu66. This led to the misinterpretation that ScSnu66-GFP loses its nuclear localization upon loss of HUB1. Having remade the hub1D SNU66-GFP strain, the authors have now observed that in budding yeast, Snu66-GFP is localized in the nucleus in both the presence and absence of Hub1 (revised Figure S4, shown here). Although this aspect of Hub1 function does not, therefore, seem to be conserved between these two yeasts, the main findings of the paper remain intact, specifically that Hub1 is required for pre-mRNA splicing in S. pombe and that its loss also affects localization of Snu66 in this yeast. Figure S4 and its legend, shown here, have also been corrected in the Supplemental Data available online. The authors regret and apologize greatly for any inconvenience and confusion that this error may have caused to others.
Figure S4. Localization of Snu66-GFP in S. cerevisiae Cells expressing the Snu66-GFP fusion protein in wild-type cells ([A] yGD319, Snu66-GFP::kanMX4) and in the Dhub1 background ([B] yGD318, Snu66-GFP::kanMX4 hub1::natMX6). The upper panel shows the cells in phase contrast, and the lower panel shows the fluorescence GFP signal of the Snu66-GFP fusion.
*Correspondence: [email protected]
DOI: 10.1016/j.cub.2006.12.004