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4977247 Immobilized protein G variants and the use thereof
PATENT ABSTRACTS
290
sample with a fluorescently labeled ligand immunologically complexed onto a solid phase supported antibody capable of exchanging the fluorescently labeled ligand with the analyte ligand in the sample and (2) measuring the fluorescence of the resulting liquid phase without measuring the fluorescence of the resulting solid phase and without separating the resulting solid phase from the resulting liquid phase.
4977080 CHEMILUMINESCENT METHODS AND A KIT THEREFOR INVOLVING A BETA-LACTAM
was characterized. Monocyte cytotoxicity inducing factor was found to be stable at pH 2 for one hour, unlike interferon-gamma, and was found to be more heat stable as well. Moreover, treatment of MCF with antisera to interferons gamma, alpha, or a combination of gamma and alpha failed to neutralize its biologic activity. MCF binds to Matrex Gel Red A. MCF eluted from this dye-ligand was found to have an apparent molecular weight of I 1,500 Daltons by gel filtration and 14,700 Daltons by SDSpolyacrylamide gel electrophoresis. MCF produced by hybridized Sezary cells appear to be neither interferon-gamma nor an altered molecular form of interferon-gamma, yet is a potent inducer of human monocyte cytotoxicity.
4977247 Dean Milbrath assigned to Minnesota Mining and Manufacturing Co Methods for the detection and measurement of compounds containing beta-lactam rings by chemiluminescence of the compounds in the presence of a chemiluminescent compound such as luminol, and for improving the sensitivity of assays using chemiluminescent reactions such as the luminol reaction utilizing compounds containing beta-lactam rings are described. A kit for conducting such chemiluminescent assays is also described.
IMMOBILIZED PROTEIN G VARIANTS AND THE USE THEREOF Stephen R Fahnestock, Timothy Lee, Marie H Wroble assigned to Genex Corporation Immobilized IgG binding proteins and the use thereof to effect affinity chromatography and separate the subclasses of IgG. Disclosed are cysteine-containing lgG binding proteins which have high binding capacity for human IgG and mouse monoclonal lgG.
4977245 METHODS AND COMPOSITIONS FOR INDUCING MONOCYTE CYTOTOXICITY C Michae Jones assigned to Board of Regents The University of Texas System The present disclosure relates to a new lymphokine molecule, referred to as Monocyte Cytotoxicity Inducing Factor (MCF), and its use as in cancer and other types of therapy. The disclosure further relates to the development of novel Sezary cell hybridomas which secrete MCF and thereby provide a ready source for MCF isolation and purification. Sezary'is Syndrome i a leukemic proliferation o f O K T 4 + lymphocytes. Sezary cells were isolated by differential centrifugation and fused to CEM.8aza.rC, an HGPRTase lacking clone of CEM. The hybrid cells were studied for their ability to produce soluble mediators of human monocyte cytotoxicity. The product of a single clone, FtF3, which bore the surface phenotype of Sezary cells,
4978608 DNA DETECTION
SYSTEM
Viola T Kung, Peter A Nagainis assigned to Molecular Devices Corporation Picogram amounts of DNA can be detected in a sample by the use of high affinity single-stranded DNA binding proteins. The assay is applicable not only to pure DNA samples but also to samples containing significant amounts of protein.
4978610 METHOD OF ASSAY EMPLOYING A MAGNETIC ELECTRODE Gordon C Forrest, Simon Rattle, Grenville Robinson, Hugh A O Hill, East Horsley, Surrey KT24 5DP, United Kingdom
290
sample with a fluorescently labeled ligand immunologically complexed onto a solid phase supported antibody capable of exchanging the fluorescently labeled ligand with the analyte ligand in the sample and (2) measuring the fluorescence of the resulting liquid phase without measuring the fluorescence of the resulting solid phase and without separating the resulting solid phase from the resulting liquid phase.
4977080 CHEMILUMINESCENT METHODS AND A KIT THEREFOR INVOLVING A BETA-LACTAM
was characterized. Monocyte cytotoxicity inducing factor was found to be stable at pH 2 for one hour, unlike interferon-gamma, and was found to be more heat stable as well. Moreover, treatment of MCF with antisera to interferons gamma, alpha, or a combination of gamma and alpha failed to neutralize its biologic activity. MCF binds to Matrex Gel Red A. MCF eluted from this dye-ligand was found to have an apparent molecular weight of I 1,500 Daltons by gel filtration and 14,700 Daltons by SDSpolyacrylamide gel electrophoresis. MCF produced by hybridized Sezary cells appear to be neither interferon-gamma nor an altered molecular form of interferon-gamma, yet is a potent inducer of human monocyte cytotoxicity.
4977247 Dean Milbrath assigned to Minnesota Mining and Manufacturing Co Methods for the detection and measurement of compounds containing beta-lactam rings by chemiluminescence of the compounds in the presence of a chemiluminescent compound such as luminol, and for improving the sensitivity of assays using chemiluminescent reactions such as the luminol reaction utilizing compounds containing beta-lactam rings are described. A kit for conducting such chemiluminescent assays is also described.
IMMOBILIZED PROTEIN G VARIANTS AND THE USE THEREOF Stephen R Fahnestock, Timothy Lee, Marie H Wroble assigned to Genex Corporation Immobilized IgG binding proteins and the use thereof to effect affinity chromatography and separate the subclasses of IgG. Disclosed are cysteine-containing lgG binding proteins which have high binding capacity for human IgG and mouse monoclonal lgG.
4977245 METHODS AND COMPOSITIONS FOR INDUCING MONOCYTE CYTOTOXICITY C Michae Jones assigned to Board of Regents The University of Texas System The present disclosure relates to a new lymphokine molecule, referred to as Monocyte Cytotoxicity Inducing Factor (MCF), and its use as in cancer and other types of therapy. The disclosure further relates to the development of novel Sezary cell hybridomas which secrete MCF and thereby provide a ready source for MCF isolation and purification. Sezary'is Syndrome i a leukemic proliferation o f O K T 4 + lymphocytes. Sezary cells were isolated by differential centrifugation and fused to CEM.8aza.rC, an HGPRTase lacking clone of CEM. The hybrid cells were studied for their ability to produce soluble mediators of human monocyte cytotoxicity. The product of a single clone, FtF3, which bore the surface phenotype of Sezary cells,
4978608 DNA DETECTION
SYSTEM
Viola T Kung, Peter A Nagainis assigned to Molecular Devices Corporation Picogram amounts of DNA can be detected in a sample by the use of high affinity single-stranded DNA binding proteins. The assay is applicable not only to pure DNA samples but also to samples containing significant amounts of protein.
4978610 METHOD OF ASSAY EMPLOYING A MAGNETIC ELECTRODE Gordon C Forrest, Simon Rattle, Grenville Robinson, Hugh A O Hill, East Horsley, Surrey KT24 5DP, United Kingdom