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Antibacterial activity of Ludwigia adscendens
Fitoterapia 76 (2005) 473 – 475 www.elsevier.com/locate/fitote
Short report
Antibacterial activity of Ludwigia adscendens Firoj Ahmed*, M.S.T. Selim, J.A. Shilpi Pharmacy Discipline, Khulna University, Khulna-9208, Bangladesh Received 21 July 2004; accepted 26 April 2005
Abstract Methanolic extract of whole plants of Ludwigia adscendens was studied for its antibacterial activity. The extract showed a broad spectrum of antibacterial activity against all the bacteria tested except Stapylococcus aureus. D 2005 Elsevier B.V. All rights reserved. Keywords: Ludwigia adscendens; Antibacterial activity
1. Plant Ludwigia adscendens L. (Onagraceae), whole plant collected in November 2002 from the district of Natore was identified in the National Herbarium of Bangladesh (Voucher No. 29751).
2. Uses in traditional medicine and previously reported activity The whole plant is antiseptic and used as a poultice in ulcers. Extract of leaves and stem possess a strong antimicrobial activity and is used against various skin diseases. Flower
* Corresponding author. Tel.: +880 41 720171 3x252; fax: +880 41 731244. E-mail address: [email protected] (F. Ahmed). 0367-326X/$ - see front matter D 2005 Elsevier B.V. All rights reserved. doi:10.1016/j.fitote.2005.04.007
474
F. Ahmed et al. / Fitoterapia 76 (2005) 473–475
petals possesses antiinflammatory activity [1]. The plant is also used as emetic, laxative, anthelmintic and antidysenteric [2].
3. Previously isolated constituents Leaves and stems contain flavonoids, terpenes, triterpenoids, phenols, tannins, alkaloids and carbohydrates [3].
4. Tested material Methanolic extract.
5. Studied activity Antibacterial activity by disc diffusion method [4,5].
6. Used microorganisms Listed in Table 1.
7. Results Reported in Table 1.
Table 1 Antibacterial activity of L. adscendens methanolic extract Bacteria
Staphylococcus aureus Staphylococcus epidermis Streptococcus pyogenes Escherichia coli Salmonella typhi Shigella boydii Shigella dysenteriae Shigella flexneri Shigella sonii Vibrio cholerae (–): No inhibition.
Zone of inhibition (mm) Methanol extract 500 Ag/disc
Kanamycin (30 Ag/disc)
– 15 13 17 20 18 20 15 17 20
30 32 40 39 30 38 34 31 31 30
F. Ahmed et al. / Fitoterapia 76 (2005) 473–475
475
8. Conclusions The methanolic extract of L. adscendens showed a broad spectrum antibacterial activity against all the bacteria tested except S. aureus. This result supports the traditional use of this plant.
Acknowledgements The authors are grateful to Mr. Hasibur Rahman, Associate Professor, Biotechnology Discipline, Khulna University, Khulna-9208, Bangladesh.
References [1] Selim MST. Phytochemical and pharmacological screening of Ludwigia adscendens L. B. Pharm project report submitted to Pharmacy Discipline. Bangladesh: Khulna University; 2003. p. 16. [2] Kirtikar KR, Basu BD. 2nd ed. Indian medicinal plants, vol. III. India7 International Book Distributors; 1987. p. 1088. [3] Ghani A. Medicinal plants of Bangladesh: chemical constituents and uses. Dhaka7 Asiatic Society of Bangladesh; 1998. p. 220. [4] Bauer AW, Kirby WMM, Sherris JC, Turck M. Am J Clin Pathol 1966;45:492. [5] Ahmed F, Rahman GMS, Das AK. Khulna University Studies 2001;32:531.
Short report
Antibacterial activity of Ludwigia adscendens Firoj Ahmed*, M.S.T. Selim, J.A. Shilpi Pharmacy Discipline, Khulna University, Khulna-9208, Bangladesh Received 21 July 2004; accepted 26 April 2005
Abstract Methanolic extract of whole plants of Ludwigia adscendens was studied for its antibacterial activity. The extract showed a broad spectrum of antibacterial activity against all the bacteria tested except Stapylococcus aureus. D 2005 Elsevier B.V. All rights reserved. Keywords: Ludwigia adscendens; Antibacterial activity
1. Plant Ludwigia adscendens L. (Onagraceae), whole plant collected in November 2002 from the district of Natore was identified in the National Herbarium of Bangladesh (Voucher No. 29751).
2. Uses in traditional medicine and previously reported activity The whole plant is antiseptic and used as a poultice in ulcers. Extract of leaves and stem possess a strong antimicrobial activity and is used against various skin diseases. Flower
* Corresponding author. Tel.: +880 41 720171 3x252; fax: +880 41 731244. E-mail address: [email protected] (F. Ahmed). 0367-326X/$ - see front matter D 2005 Elsevier B.V. All rights reserved. doi:10.1016/j.fitote.2005.04.007
474
F. Ahmed et al. / Fitoterapia 76 (2005) 473–475
petals possesses antiinflammatory activity [1]. The plant is also used as emetic, laxative, anthelmintic and antidysenteric [2].
3. Previously isolated constituents Leaves and stems contain flavonoids, terpenes, triterpenoids, phenols, tannins, alkaloids and carbohydrates [3].
4. Tested material Methanolic extract.
5. Studied activity Antibacterial activity by disc diffusion method [4,5].
6. Used microorganisms Listed in Table 1.
7. Results Reported in Table 1.
Table 1 Antibacterial activity of L. adscendens methanolic extract Bacteria
Staphylococcus aureus Staphylococcus epidermis Streptococcus pyogenes Escherichia coli Salmonella typhi Shigella boydii Shigella dysenteriae Shigella flexneri Shigella sonii Vibrio cholerae (–): No inhibition.
Zone of inhibition (mm) Methanol extract 500 Ag/disc
Kanamycin (30 Ag/disc)
– 15 13 17 20 18 20 15 17 20
30 32 40 39 30 38 34 31 31 30
F. Ahmed et al. / Fitoterapia 76 (2005) 473–475
475
8. Conclusions The methanolic extract of L. adscendens showed a broad spectrum antibacterial activity against all the bacteria tested except S. aureus. This result supports the traditional use of this plant.
Acknowledgements The authors are grateful to Mr. Hasibur Rahman, Associate Professor, Biotechnology Discipline, Khulna University, Khulna-9208, Bangladesh.
References [1] Selim MST. Phytochemical and pharmacological screening of Ludwigia adscendens L. B. Pharm project report submitted to Pharmacy Discipline. Bangladesh: Khulna University; 2003. p. 16. [2] Kirtikar KR, Basu BD. 2nd ed. Indian medicinal plants, vol. III. India7 International Book Distributors; 1987. p. 1088. [3] Ghani A. Medicinal plants of Bangladesh: chemical constituents and uses. Dhaka7 Asiatic Society of Bangladesh; 1998. p. 220. [4] Bauer AW, Kirby WMM, Sherris JC, Turck M. Am J Clin Pathol 1966;45:492. [5] Ahmed F, Rahman GMS, Das AK. Khulna University Studies 2001;32:531.